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Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation

Received: 4 January 2021     Accepted: 18 January 2021     Published: 25 January 2021
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Abstract

Sirtuin involves in cellular processes to adapt to starvation in the endocrine and metabolic systems. We examined Sir2B function during early Dictyostelium development upon starvation. GFP-tagged Sir2B was expressed and the immunoreactivity was detected a punctate pattern near cell surface. Sir2B mRNA was expressed in vegetative cells and peaked at 2 h after starvation. Ectopic expression of Sir2B enhanced aggregate formation with increased expressions of cell adhesion molecules such as cadA, csaA, TgrB1, and TgrC1 at 8h compared to those in control cells. Sir2B null-mutant cells (Sir2B KO cells) were generated and they formed aggregates smaller than those of wild-type cells after 24 h. Because cell-cell adhesion affects aggregation size, cell cohesion assay of Sir2B KO cells showed cell-cell adhesion impairment at 8 and 10 h during development, and the expressions of four cell adhesion molecules were reduced in Sir2B KO cells compared to wild-type cells until 8 h after starvation. When RNAi-mediated cadA or csaA knockdown cells (cadA KD or csaA KD cells) were generated, csaA KD cells formed smaller aggregates and affected the expressions of other cell adhesion molecules to reduce, but cadA KD cells did not show to affect aggregation size and cell adhesion molecules expressions apparently. These results suggest that Sir2B is involved in csaA expression and affects other cell adhesion molecules during early development upon starvation.

Published in Cell Biology (Volume 9, Issue 1)
DOI 10.11648/j.cb.20210901.11
Page(s) 1-6
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Sirtuin, Sir2B, Cell Adhesion, Development

References
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  • APA Style

    Hideo Taniura, Shuhei Soeda, Ai Fujii, Mio Morioka, Minori Nakamura, et al. (2021). Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation. Cell Biology, 9(1), 1-6. https://doi.org/10.11648/j.cb.20210901.11

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    ACS Style

    Hideo Taniura; Shuhei Soeda; Ai Fujii; Mio Morioka; Minori Nakamura, et al. Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation. Cell Biol. 2021, 9(1), 1-6. doi: 10.11648/j.cb.20210901.11

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    AMA Style

    Hideo Taniura, Shuhei Soeda, Ai Fujii, Mio Morioka, Minori Nakamura, et al. Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation. Cell Biol. 2021;9(1):1-6. doi: 10.11648/j.cb.20210901.11

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  • @article{10.11648/j.cb.20210901.11,
      author = {Hideo Taniura and Shuhei Soeda and Ai Fujii and Mio Morioka and Minori Nakamura and Yui Sano},
      title = {Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation},
      journal = {Cell Biology},
      volume = {9},
      number = {1},
      pages = {1-6},
      doi = {10.11648/j.cb.20210901.11},
      url = {https://doi.org/10.11648/j.cb.20210901.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20210901.11},
      abstract = {Sirtuin involves in cellular processes to adapt to starvation in the endocrine and metabolic systems. We examined Sir2B function during early Dictyostelium development upon starvation. GFP-tagged Sir2B was expressed and the immunoreactivity was detected a punctate pattern near cell surface. Sir2B mRNA was expressed in vegetative cells and peaked at 2 h after starvation. Ectopic expression of Sir2B enhanced aggregate formation with increased expressions of cell adhesion molecules such as cadA, csaA, TgrB1, and TgrC1 at 8h compared to those in control cells. Sir2B null-mutant cells (Sir2B KO cells) were generated and they formed aggregates smaller than those of wild-type cells after 24 h. Because cell-cell adhesion affects aggregation size, cell cohesion assay of Sir2B KO cells showed cell-cell adhesion impairment at 8 and 10 h during development, and the expressions of four cell adhesion molecules were reduced in Sir2B KO cells compared to wild-type cells until 8 h after starvation. When RNAi-mediated cadA or csaA knockdown cells (cadA KD or csaA KD cells) were generated, csaA KD cells formed smaller aggregates and affected the expressions of other cell adhesion molecules to reduce, but cadA KD cells did not show to affect aggregation size and cell adhesion molecules expressions apparently. These results suggest that Sir2B is involved in csaA expression and affects other cell adhesion molecules during early development upon starvation.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation
    AU  - Hideo Taniura
    AU  - Shuhei Soeda
    AU  - Ai Fujii
    AU  - Mio Morioka
    AU  - Minori Nakamura
    AU  - Yui Sano
    Y1  - 2021/01/25
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    N1  - https://doi.org/10.11648/j.cb.20210901.11
    DO  - 10.11648/j.cb.20210901.11
    T2  - Cell Biology
    JF  - Cell Biology
    JO  - Cell Biology
    SP  - 1
    EP  - 6
    PB  - Science Publishing Group
    SN  - 2330-0183
    UR  - https://doi.org/10.11648/j.cb.20210901.11
    AB  - Sirtuin involves in cellular processes to adapt to starvation in the endocrine and metabolic systems. We examined Sir2B function during early Dictyostelium development upon starvation. GFP-tagged Sir2B was expressed and the immunoreactivity was detected a punctate pattern near cell surface. Sir2B mRNA was expressed in vegetative cells and peaked at 2 h after starvation. Ectopic expression of Sir2B enhanced aggregate formation with increased expressions of cell adhesion molecules such as cadA, csaA, TgrB1, and TgrC1 at 8h compared to those in control cells. Sir2B null-mutant cells (Sir2B KO cells) were generated and they formed aggregates smaller than those of wild-type cells after 24 h. Because cell-cell adhesion affects aggregation size, cell cohesion assay of Sir2B KO cells showed cell-cell adhesion impairment at 8 and 10 h during development, and the expressions of four cell adhesion molecules were reduced in Sir2B KO cells compared to wild-type cells until 8 h after starvation. When RNAi-mediated cadA or csaA knockdown cells (cadA KD or csaA KD cells) were generated, csaA KD cells formed smaller aggregates and affected the expressions of other cell adhesion molecules to reduce, but cadA KD cells did not show to affect aggregation size and cell adhesion molecules expressions apparently. These results suggest that Sir2B is involved in csaA expression and affects other cell adhesion molecules during early development upon starvation.
    VL  - 9
    IS  - 1
    ER  - 

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Author Information
  • Laboratory of Neurochemistry, College of Pharmacy, Ritsumeikan University, Kusatsu, Japan

  • Laboratory of Neurochemistry, College of Pharmacy, Ritsumeikan University, Kusatsu, Japan

  • Laboratory of Neurochemistry, College of Pharmacy, Ritsumeikan University, Kusatsu, Japan

  • Laboratory of Neurochemistry, College of Pharmacy, Ritsumeikan University, Kusatsu, Japan

  • Laboratory of Neurochemistry, College of Pharmacy, Ritsumeikan University, Kusatsu, Japan

  • Laboratory of Neurochemistry, College of Pharmacy, Ritsumeikan University, Kusatsu, Japan

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