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D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (Rattus Novergicus) Exposed to X-Ray

Received: 12 March 2017     Accepted: 27 May 2017     Published: 18 July 2017
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Abstract

Oxidative stress mechanisms are involved in xenobiotic-induced testicular dysfunctions which consequently lead to male infertility, however, antioxidants work like a defense system, disarming free radicals. Therefore, this study is aimed at investigating the maintenance of testicular integrity using D-Riboce-L-cysteine on X–ray induced testicular damage in adult wistar rats (Rattus Novergicus). A total of 20 male rats were randomly selected into Five (5) groups of Four (4) animal each. Control animals received only water while treated animals include: animals induced with X–ray only; around the pelvic and perineum region at about 95 kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD), animals treated with D-Riboce-L-cysteine at 30 mg/kg body weight of D-Riboce-L-cysteine before exposed to 95 kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD) of X-ray, animals treated with 95kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD) of X-ray per animal before receiving 30 mg/kg body weight of D-Riboce-L-cysteine and animals treated with 30 mg/kg body weight of D-Riboce-L-cysteine only. After 21 days of treatments, the animals were sacrificed, and the testes were excised following abdominal incision, fixed in Bouin’s fluids for histological observations and right testis was homogenized in 5% sucrose solution for determination of enzymes of carbohydrates metabolism. Sperm was obtained from the caudal part of the epididymis for analysis of sperm characteristics. Reduced sperm count, abnormal morphology and significant (p < 0.05) higher non motile sperm characterized the animals expose to X-ray. However, sperm characteristics was maintained in control animals (p < 0.05) and animals treated with D-Riboce-L-cysteine only. Reduced activities in enzyme of carbohydrate metabolism (G-6-PDH) and significant increase in the level of lipid peroxidation shown by the activities of MDA in the X-ray treated groups compared to animals treated with D-Riboce-L-cysteine (p < 0.05) and the control animals. Abnormal widening of the interstitial space, loss of the basal laminal, degeneration in spermatogonia with vacuolation. Loss of germinal epithelium of the seminiferous tubules were also observed in animals expose to X-ray. Exposure to X-ray disrupts spermatogenesis by disruption and depletion of the spermatids and spermatogonia population, which caused increase in testicular tissue damage and consequently, altered the sperm characteristics. D-Riboce-L-cysteine clearly demonstrated maintenance of testicular integrity and enhance sperm characteristics; indication of fertility enhancing ability.

Published in Cell Biology (Volume 5, Issue 4)
DOI 10.11648/j.cb.20170504.12
Page(s) 38-44
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

X-Ray, Testes, Free Radicals, Antioxidants and Wistar Rats

References
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    Dare Babatunde Joseph, Olayemi Olamide Samuel, Falana Benedict Abiola, Duru Francis I. Ogueri, Osinubi Abraham A. A. (2017). D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (Rattus Novergicus) Exposed to X-Ray. Cell Biology, 5(4), 38-44. https://doi.org/10.11648/j.cb.20170504.12

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    Dare Babatunde Joseph; Olayemi Olamide Samuel; Falana Benedict Abiola; Duru Francis I. Ogueri; Osinubi Abraham A. A. D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (Rattus Novergicus) Exposed to X-Ray. Cell Biol. 2017, 5(4), 38-44. doi: 10.11648/j.cb.20170504.12

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    AMA Style

    Dare Babatunde Joseph, Olayemi Olamide Samuel, Falana Benedict Abiola, Duru Francis I. Ogueri, Osinubi Abraham A. A. D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (Rattus Novergicus) Exposed to X-Ray. Cell Biol. 2017;5(4):38-44. doi: 10.11648/j.cb.20170504.12

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  • @article{10.11648/j.cb.20170504.12,
      author = {Dare Babatunde Joseph and Olayemi Olamide Samuel and Falana Benedict Abiola and Duru Francis I. Ogueri and Osinubi Abraham A. A.},
      title = {D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (Rattus Novergicus) Exposed to X-Ray},
      journal = {Cell Biology},
      volume = {5},
      number = {4},
      pages = {38-44},
      doi = {10.11648/j.cb.20170504.12},
      url = {https://doi.org/10.11648/j.cb.20170504.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20170504.12},
      abstract = {Oxidative stress mechanisms are involved in xenobiotic-induced testicular dysfunctions which consequently lead to male infertility, however, antioxidants work like a defense system, disarming free radicals. Therefore, this study is aimed at investigating the maintenance of testicular integrity using D-Riboce-L-cysteine on X–ray induced testicular damage in adult wistar rats (Rattus Novergicus). A total of 20 male rats were randomly selected into Five (5) groups of Four (4) animal each. Control animals received only water while treated animals include: animals induced with X–ray only; around the pelvic and perineum region at about 95 kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD), animals treated with D-Riboce-L-cysteine at 30 mg/kg body weight of D-Riboce-L-cysteine before exposed to 95 kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD) of X-ray, animals treated with 95kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD) of X-ray per animal before receiving 30 mg/kg body weight of D-Riboce-L-cysteine and animals treated with 30 mg/kg body weight of D-Riboce-L-cysteine only. After 21 days of treatments, the animals were sacrificed, and the testes were excised following abdominal incision, fixed in Bouin’s fluids for histological observations and right testis was homogenized in 5% sucrose solution for determination of enzymes of carbohydrates metabolism. Sperm was obtained from the caudal part of the epididymis for analysis of sperm characteristics. Reduced sperm count, abnormal morphology and significant (p < 0.05) higher non motile sperm characterized the animals expose to X-ray. However, sperm characteristics was maintained in control animals (p < 0.05) and animals treated with D-Riboce-L-cysteine only. Reduced activities in enzyme of carbohydrate metabolism (G-6-PDH) and significant increase in the level of lipid peroxidation shown by the activities of MDA in the X-ray treated groups compared to animals treated with D-Riboce-L-cysteine (p < 0.05) and the control animals. Abnormal widening of the interstitial space, loss of the basal laminal, degeneration in spermatogonia with vacuolation. Loss of germinal epithelium of the seminiferous tubules were also observed in animals expose to X-ray. Exposure to X-ray disrupts spermatogenesis by disruption and depletion of the spermatids and spermatogonia population, which caused increase in testicular tissue damage and consequently, altered the sperm characteristics. D-Riboce-L-cysteine clearly demonstrated maintenance of testicular integrity and enhance sperm characteristics; indication of fertility enhancing ability.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - D-Riboce-L-Cystein Maintained Testicular Integrity in Rats Model (Rattus Novergicus) Exposed to X-Ray
    AU  - Dare Babatunde Joseph
    AU  - Olayemi Olamide Samuel
    AU  - Falana Benedict Abiola
    AU  - Duru Francis I. Ogueri
    AU  - Osinubi Abraham A. A.
    Y1  - 2017/07/18
    PY  - 2017
    N1  - https://doi.org/10.11648/j.cb.20170504.12
    DO  - 10.11648/j.cb.20170504.12
    T2  - Cell Biology
    JF  - Cell Biology
    JO  - Cell Biology
    SP  - 38
    EP  - 44
    PB  - Science Publishing Group
    SN  - 2330-0183
    UR  - https://doi.org/10.11648/j.cb.20170504.12
    AB  - Oxidative stress mechanisms are involved in xenobiotic-induced testicular dysfunctions which consequently lead to male infertility, however, antioxidants work like a defense system, disarming free radicals. Therefore, this study is aimed at investigating the maintenance of testicular integrity using D-Riboce-L-cysteine on X–ray induced testicular damage in adult wistar rats (Rattus Novergicus). A total of 20 male rats were randomly selected into Five (5) groups of Four (4) animal each. Control animals received only water while treated animals include: animals induced with X–ray only; around the pelvic and perineum region at about 95 kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD), animals treated with D-Riboce-L-cysteine at 30 mg/kg body weight of D-Riboce-L-cysteine before exposed to 95 kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD) of X-ray, animals treated with 95kv, 12.5 milliampere-seconds (mA.s), 50 focal field distance (FFD) of X-ray per animal before receiving 30 mg/kg body weight of D-Riboce-L-cysteine and animals treated with 30 mg/kg body weight of D-Riboce-L-cysteine only. After 21 days of treatments, the animals were sacrificed, and the testes were excised following abdominal incision, fixed in Bouin’s fluids for histological observations and right testis was homogenized in 5% sucrose solution for determination of enzymes of carbohydrates metabolism. Sperm was obtained from the caudal part of the epididymis for analysis of sperm characteristics. Reduced sperm count, abnormal morphology and significant (p < 0.05) higher non motile sperm characterized the animals expose to X-ray. However, sperm characteristics was maintained in control animals (p < 0.05) and animals treated with D-Riboce-L-cysteine only. Reduced activities in enzyme of carbohydrate metabolism (G-6-PDH) and significant increase in the level of lipid peroxidation shown by the activities of MDA in the X-ray treated groups compared to animals treated with D-Riboce-L-cysteine (p < 0.05) and the control animals. Abnormal widening of the interstitial space, loss of the basal laminal, degeneration in spermatogonia with vacuolation. Loss of germinal epithelium of the seminiferous tubules were also observed in animals expose to X-ray. Exposure to X-ray disrupts spermatogenesis by disruption and depletion of the spermatids and spermatogonia population, which caused increase in testicular tissue damage and consequently, altered the sperm characteristics. D-Riboce-L-cysteine clearly demonstrated maintenance of testicular integrity and enhance sperm characteristics; indication of fertility enhancing ability.
    VL  - 5
    IS  - 4
    ER  - 

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Author Information
  • Anatomy Department, Osun State University, Osogbo, Nigeria

  • Anatomy Department, Osun State University, Osogbo, Nigeria

  • Anatomy Department, Osun State University, Osogbo, Nigeria

  • Anatomy Department, University of Lagos, Lagos, Nigeria

  • Anatomy Department, University of Lagos, Lagos, Nigeria

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