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LC-MS Analysis of Phenolic Acids, Flavonoids and Betanin from Spineless Opuntia ficus-indica Fruits

Received: 16 February 2017     Accepted: 11 March 2017     Published: 1 April 2017
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Abstract

Opuntia ficus-indica belongs to the Cactaceae family and is widespread in semi-arid and arid regions. Cactus pears are known for their health promoting properties which are due to a variety of bioactive molecules. This study aims to identify and quantify phenolic acids, flavonoids and betanin from spineless Opuntia ficus-indica fruits. Fresh mature samples were crushed, and extracted with 50% aqueous ethanol. The identification process was carried out using a Shimadzu high performance liquid chromagraph equipped with a quadrupole mass spectrum. Quantification was made using calibration curves of analytical standards. 9 phenolic acids, 1 flavan-3-ol, 2 flavanones, 3 flavonols, 6 flavones and 2 betacyanins were identified. Important levels of betanin, quinic acid, cirsiliol, acacetin, transcinnamic acid, rutin and naringin were calculated. Our results indicated that Opuntia ficus-indica fruits are an important dietary source of phenolic compounds and betalains with high antioxidant capacity.

Published in Cell Biology (Volume 5, Issue 2)
DOI 10.11648/j.cb.20170502.12
Page(s) 17-28
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Opuntia ficus-indica, Cactus Pear, Polyphenols, Betanin

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Cite This Article
  • APA Style

    Imen Belhadj Slimen, Mahmoud Mabrouk, Chaabane Hanène, Taha Najar, Manef Abderrabba. (2017). LC-MS Analysis of Phenolic Acids, Flavonoids and Betanin from Spineless Opuntia ficus-indica Fruits. Cell Biology, 5(2), 17-28. https://doi.org/10.11648/j.cb.20170502.12

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    ACS Style

    Imen Belhadj Slimen; Mahmoud Mabrouk; Chaabane Hanène; Taha Najar; Manef Abderrabba. LC-MS Analysis of Phenolic Acids, Flavonoids and Betanin from Spineless Opuntia ficus-indica Fruits. Cell Biol. 2017, 5(2), 17-28. doi: 10.11648/j.cb.20170502.12

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    AMA Style

    Imen Belhadj Slimen, Mahmoud Mabrouk, Chaabane Hanène, Taha Najar, Manef Abderrabba. LC-MS Analysis of Phenolic Acids, Flavonoids and Betanin from Spineless Opuntia ficus-indica Fruits. Cell Biol. 2017;5(2):17-28. doi: 10.11648/j.cb.20170502.12

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  • @article{10.11648/j.cb.20170502.12,
      author = {Imen Belhadj Slimen and Mahmoud Mabrouk and Chaabane Hanène and Taha Najar and Manef Abderrabba},
      title = {LC-MS Analysis of Phenolic Acids, Flavonoids and Betanin from Spineless Opuntia ficus-indica Fruits},
      journal = {Cell Biology},
      volume = {5},
      number = {2},
      pages = {17-28},
      doi = {10.11648/j.cb.20170502.12},
      url = {https://doi.org/10.11648/j.cb.20170502.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20170502.12},
      abstract = {Opuntia ficus-indica belongs to the Cactaceae family and is widespread in semi-arid and arid regions. Cactus pears are known for their health promoting properties which are due to a variety of bioactive molecules. This study aims to identify and quantify phenolic acids, flavonoids and betanin from spineless Opuntia ficus-indica fruits. Fresh mature samples were crushed, and extracted with 50% aqueous ethanol. The identification process was carried out using a Shimadzu high performance liquid chromagraph equipped with a quadrupole mass spectrum. Quantification was made using calibration curves of analytical standards. 9 phenolic acids, 1 flavan-3-ol, 2 flavanones, 3 flavonols, 6 flavones and 2 betacyanins were identified. Important levels of betanin, quinic acid, cirsiliol, acacetin, transcinnamic acid, rutin and naringin were calculated. Our results indicated that Opuntia ficus-indica fruits are an important dietary source of phenolic compounds and betalains with high antioxidant capacity.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - LC-MS Analysis of Phenolic Acids, Flavonoids and Betanin from Spineless Opuntia ficus-indica Fruits
    AU  - Imen Belhadj Slimen
    AU  - Mahmoud Mabrouk
    AU  - Chaabane Hanène
    AU  - Taha Najar
    AU  - Manef Abderrabba
    Y1  - 2017/04/01
    PY  - 2017
    N1  - https://doi.org/10.11648/j.cb.20170502.12
    DO  - 10.11648/j.cb.20170502.12
    T2  - Cell Biology
    JF  - Cell Biology
    JO  - Cell Biology
    SP  - 17
    EP  - 28
    PB  - Science Publishing Group
    SN  - 2330-0183
    UR  - https://doi.org/10.11648/j.cb.20170502.12
    AB  - Opuntia ficus-indica belongs to the Cactaceae family and is widespread in semi-arid and arid regions. Cactus pears are known for their health promoting properties which are due to a variety of bioactive molecules. This study aims to identify and quantify phenolic acids, flavonoids and betanin from spineless Opuntia ficus-indica fruits. Fresh mature samples were crushed, and extracted with 50% aqueous ethanol. The identification process was carried out using a Shimadzu high performance liquid chromagraph equipped with a quadrupole mass spectrum. Quantification was made using calibration curves of analytical standards. 9 phenolic acids, 1 flavan-3-ol, 2 flavanones, 3 flavonols, 6 flavones and 2 betacyanins were identified. Important levels of betanin, quinic acid, cirsiliol, acacetin, transcinnamic acid, rutin and naringin were calculated. Our results indicated that Opuntia ficus-indica fruits are an important dietary source of phenolic compounds and betalains with high antioxidant capacity.
    VL  - 5
    IS  - 2
    ER  - 

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Author Information
  • Department of Animal, Food and Halieutic Resources, National Agronomic Institute of Tunisia, Mahragen City, Tunisia

  • Central Laboratory, Institute of Arid Regions, Medenine, Tunisia

  • Laboratory of Bioagressors and Integrated Protection in Agriculture, National Institute of Agronomy of Tunisia, Mahragen City, Tunisia

  • Department of Animal, Food and Halieutic Resources, National Agronomic Institute of Tunisia, Mahragen City, Tunisia

  • Laboratory of Materials, Molecules and Applications, Preparatory Institute for Scientific and Technical Studies, La Marsa, Tunisia

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