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Effect of Lycopene on Altered Kidney Antioxidant Enzymes Activity and Functions in Streptozotocin-Induced Diabetic Wistar Rats

Received: 22 December 2014     Accepted: 5 January 2015     Published: 12 January 2015
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Abstract

The present study assessed the effects of lycopene on kidney antioxidant enzymes activities and functions in streptozotocin-induced diabetic Wistar rats.Diabetes was induced in animals by single intra-peritoneal injection of streptozotocin. Thereafter the animals were randomly assigned into the following groups: Group I and II (Normal control + olive oil and Diabetic control + olive oil)while Group III to VI were treated with (10, 20 and 40 mg/kg of lycopene and 2 mg/kg glibenclamide) respectively. Alltreatments were givenonce daily orally for four weeks. Results obtained showed that blood glucose was significantly (P < 0.05) reduced. MDAconcentration was reduced in kidney tissue, with increased activities of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase) in diabetic animals administered with lycopene when compared with diabetic control group.There was significant (P < 0.05) increase in the level of serum sodium ion and reduction in serum urea level in diabetic rats treated with lycopene when compared with the diabetic control group. Histological findings showed improved renal architecture as reflected by reduced glomerular and tubular necrosisin all treated groups when compared with control group. It can be concluded that lycopene protects against diabetes-induced kidney damage through elevation of endogenous antioxidant enzymes and improved renal dysfunction in diabetic animals.

Published in Cell Biology (Volume 3, Issue 1)
DOI 10.11648/j.cb.20150301.11
Page(s) 1-13
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Diabetes Mellitus, Lycopene, Streptozotocin, Electrolytes, Antioxidants, Kidney

References
[1] Rossing P, Diabetic nephropathy: Worldwide epidemic and effects of currenttreatment on natural history. Current Diabetes Reports2006;6:479-83
[2] Zelmanovitz T, Gerchman F, Balthazar A, Thomazelli F, Matos J, and Canani L,Diabetic Nephropathy. Diabetology and Metabolic Syndrome2009;1:10-26
[3] Djordjević V, Hypertension and nephropathy in diabetes mellitus: What is inherited and what is acquired? Nephrology Dialysis Transplantation2001;16(suppl 6):92-93.
[4] Kalant N,Diabetic glomerulosclerosis: Current status. Canadian Medical AssociationJournal1978;119:146-53
[5] Nangaku M, Mechanisms of Tubulointerstitial Injury in the Kidney: Final Common Pathways to End-stage Renal Failure. Internal Medicine2004;43:9-17.
[6] Forbes JM, Coughlan MT,and Cooper ME, Oxidative Stress as a Major Culprit in Kidney Disease in Diabetes. Diabetes2008;57:1446-54.
[7] Ceriello A, Dello- Russo P, Amstad P,and Cerutti P, High glucose inducesantioxidant enzymes in human endothelial cells in culture; Evidence linkinghyperglycemia and oxidative stress. Diabetes1996;45:471-477.
[8] Hodgkinson AD, Bartlett T, Oates PJ, Millward BA, andDemaine AG. The Response of Antioxidant Genes to Hyperglycemia Is Abnormal in Patients With Type 1Diabetes and Diabetic Nephropathy. Diabetes2003;52:846-51.
[9] Maritim AC, Sanders RA Watkins JB, Diabetes, oxidative stress, andantioxidants: A review. Journal of Biochemical and Molecular Toxicology 2003b;17:24-38.
[10] Sih MT, Arpeeta S, Judy BD, Oxidative Stress and Novel AntioxidantApproachesto Reduce Diabetic Complications, Oxidative Stress and Diseases, Dr. VolodymyrLushchak (Ed.), InTech, Available from: http://www.intechopen.com/books/oxidativestress-anddiseases/oxidative-stress-and-novel-antioxidant-approaches-to-reduce-diabetic complications, 2012.
[11] Kakimoto M, Inoguchi T, Sonta T, Yu HY, Imamura M,Etoh T. et al.Accumulation of 8- hydroxy-2'-deoxyguanosine and mitochondrial DNA deletion inkidney of diabetic rats. Diabetes2002;51:1588-95
[12] Fenercioglu AK, Saler T, Genc E, Sabuncu H, andAltuntas Y, The effects of polyphenol-containing antioxidants on oxidative stress and lipid peroxidation in Type 2 diabetes mellitus without complications. Journal of Endocrinology andInvestigation2010; 33(2):118-124.
[13] Neri S, Calvagno S, Mauceri B, Misseri M, Tsami A, Vecchio C, Mastrosimone G, Di Pino, A, Maiorca D, Judica A, Romano G, Rizzotto A, and Signorelli S,Effects of antioxidants on postprandial oxidative stress and endothelial dysfunction in subjects with impaired glucose tolerance and type 2 diabetes. European journal of Nutrition2010; 49 (7):409-416.
[14] TrevithickJ, Massel D, Robertson JM, Tomany S, and Wall R. Model study of AREDS antioxidant supplementation of AMD compared to Visudyne: a dominant strategy? Ophthalmic Epidemiology2004; 11(5):337-346.
[15] KantoffP, Prevention, Complementary Therapies, and New Scientific Developments in the Field of Prostate Cancer. Rev. Urol.2006; 8(Suppl 2), S9-S14.
[16] Sesso HD, Liu S, Gaziano JM, and Buring JE, Dietary Lycopene, Tomato-Based Food Products and Cardiovascular Disease in Women. Journal ofNutrition2003; 133, 2336- 2341.
[17] Mozaffarieh M, Sacu S andWedrich A, The Role of the Carotenoids, Lutein and Zeaxanthin, in Protecting Against Age-related Macular Degeneration: A Review Basedon Controversial Evidence.Nutr. J. 2003; 2: 20.
[18] Carlier C, Coste J, Etchepare M, Périquet B, and Amédée-Manesme O,A Randomised Controlled Trial to Test Equivalence Between RetinylPalmitate and BetaCarotene for Vitamin A Deficiency. BMJ 1993; 307(6912):1106-1110.
[19] Sevim ÇY,Fatmagül Y,and Ebubekir C,Effect of Lycopene Application in Rats with Experimental Diabetes Using Lipoprotein, Paraoxonase and Cytokines Journal of MembraneBiology 2013; 246: 621–626
[20] Rao AV, and Agrawal S,Role of lycopene as antioxidant carotenoid in the preventionof chronic diseases, a review. Nutrition Research1999; 19: 305-323.
[21] Bramley P M, Regulation of carotenoid formation during tomato fruit ripening and development. Journal of Experimental Botany2002; 53 (377): 2107-2113.
[22] Betty K, Charlotta T, Mary CH,and McKeon T A, Fatty acid and carotenoidcomposition of Gac (MomordicacochinchinensisSpreng) fruit. Journal of Agricultureand Food Chemistry2004; 52:274-279.
[23] [23] Ogundeji T, Ayo J O, Aluwong T, and Mohammed A,Behavioural and haematological studies on effects of lycopene in Wistar rats subjected to psychological stress. Journal of Neuroscience and Behavioral Health2013;5(2):30-35.
[24] [24] MohammedA, Tanko Y, Okasha M A, Magaji R A, and Yaro AH, Effects ofaqueous leaves extract of Ocimumgratissimum on bloodglucose levels of streptozocininduced diabetic wistar rats. African Journal of Biotechnology 2007;6:2087-2090.
[25] [25] Beach EF, and Turner JJ. An enzymatic method for glucose determination uptake in bodyfluids. Clinical Chemistry1958; 4:462-468.
[26] [26] Placer ZA,Cushmann L, and Johnson BC,Estimation of products of lipid peroxidation in biochemical systems. Analytical Biochemistry 1996; 16: 359-364.
[27] [27] Suzuki K, Measurement of Mn-SOD and Cu, Zn-SOD. In: Taniguchi, N., Gutteridge, J. (Eds.), Experimental Protocols for Reactive Oxygen and Nitrogen Species. Oxford University Press, U.K. 2000; Pp. 91–95.
[28] [28] Aebi H, Catalase in vitro.Methods in Enzymology 1984; 105, 121– 126.
[29] [29] Flohe LA,and Gunzler WA, Assays for glutathione peroxidase. Methods inEnzymology 1984;105:114– 120.
[30] [30] Tietz NW, Prude EL, and Sirgard-Anderson O,Textbook of clinical chemistry. Edited by Burtis C.A. AshwoodE.R.andBruns DE. WB Saunders Company, Philadelphia, London 1994; Pp 1354 – 1374.
[31] [31] Vogel A I, A Textbook of Quantitative Inorganic Analysis. LongmanGroup Ltd. London. 3rd Edition, 1960; Pp 882-885.
[32] [32] Segal M A, A rapid electrotitimetricmethod for determining CO2 combining power in plasma or serum. American Journal of Clinical Pathology 1955;25(10): 1212-1216.
[33] [33] Schales O, Schales S, A simple and accurate method for the determination of chloride in biological fluids.Journal of Biochemistry1941; 140, 879-884.
[34] [34] Krishna D, Rao S, Satyanarayana ML, Serum insulin levels and lipid profiles of streptozotocin induced diabetic wistar rats. Journal of Indian Veterinary Association, Kerala2012; 10 (2):22-26.
[35] [35] Szkudelski T, The mechanism of alloxan and streptozotocin action in β-cells of the rat pancreas. Physiological Research 2001;50(6):537-546.
[36] [36] Duzguner V, Kucukgul A, Erdogan S, Celik S, andSahinK, Effect of lycopene administration on plasma glucose, oxidative stress and body weight in streptozotocin diabetic rats. Journal of Applied Animal Research 2008; 33: 17–20.
[37] [37] Kuhad A, Sethi R, Chopra S,Lycopene attenuates diabetes-associated cognitive decline in rats. Life Science 2008; 83(3-4):128-134.
[38] [38] Ali MM,and Agha FC, Amelioration of streptozotocin-induced diabetes mellitus, oxidative stress and dyslipidemia in rats by tomato extract lycopene. Scandinavian Journal of Clinical and Laboratory Investigation 2009; 69(3): 371-379.
[39] [39] Aydin M, and Celik S,Effects of lycopene on plasma glucose, insulin levels, oxidative stress, and body weights of streptozotocin-induced diabetic rats. Turkish Journal of Medical Sciences2012; 42 (Sup.2): 1406-1413.
[40] [40] Giacco F, and Brownlee M, Oxidative stressand diabetic complications. Circulation Research2010; 107(9):1058- 1070.
[41] [41] Tsuruta R, Fujita M, Ono T, Koda Y, Koga Y, Yamamoto T, Nanba M, Shitara M, Kasaoka S, Maruyama I, Yuasa M, and Maekawa T, Hyperglycemia enhances excessive superoxide anion radical generation, oxidative stress, early inflammation, and endothelial injury in forebrain ischemia/reperfusion rats. Brain research2010; 1309:155-163.
[42] [42] Bose KSC, and Agrawal B K,Effect of Long Term Supplementation of Tomatoes (Cooked) on Levels of Antioxidant Enzymes, Lipid Peroxidation Rate, Lipid Profile and GlycatedHaemoglobin in Type 2 Diabetes Mellitus. West Indian Medical Journal 2006; 55(4): 274-278.
[43] [43] Atessahin A, Yilmaz S, Karahan I, Ceribas A O, and Karauglu A, Effects oflycopene against cisplant-induced nephrotoxicity and oxidative stress in rats. Toxicology2005; 212: 116-123.
[44] [44] Malomo SO, Toxicological implication of ceftriaxone administration in rats. Nigerian JournalBiochemistry and Molecular Biology2000; 15(1): 33-38.
[45] [45] Yakubu MT, Salau I O and Muhammad N O, Phosphatase activities in selected rat tissues following repeated administration of ranitidine. Nigerian Journal of Biochemistry and Molecular Biology2003b;18(1): 21-24.
[46] [46] Akanji M A andYakubu M T,α- Tocopherol protects against metabisulphite –induced tissue damage in rats. Nigerian Journal of Biochemistry and Molecular Biology2000; 15: 179-183.
[47] [47] Bukan N, Sancak B, Yavuz O, Tutkin CF, Ozcelikay T, andAltan N, Lipid peroxidation and Scavenging Levels in the Liver of streptozotocin-induced Diabetic rats.Indian Journal of Biochemistry and Biophysics2003; 40: 447-450.
[48] [48] Siddiqui MH, Al-Whaibi MH, and Basalah MO, Role of nitric oxide in tolerance ofplants to abiotic stress.Protoplasma2011; 248: 447-455
[49] [49] Erejuwa OO, Oxidative Stress in diabetes mellitus: is there a role for hypoglycaemicdrugs and/or antioxidants? Oxidative stress and diseases, Volodymyr I. Lushchak and Dmytro V. Gospodaryov (Ed.), ISBN: 978-953-51-0552-7, 2012; InTech, Available from:http://www.intechopen.com/books/oxidative-stress-and-diseases/oxidative-stress in diabetes
[50] [50] Al-Faris NA, Al-Sawadi AD, and Alokail MS, Effect of Samh seedssupplementation (MesembryanthemumforsskaleiHochst) on liver enzymes and lipid profiles of streptozotocin (STZ)-induced diabetic Wistar rats. Saudi Journal of Biological Sciences 2010; 17:23-28.
[51] [51] Kumawat M, Pahwa MB, Gahlant VS, and Singh N, Status of antioxidant enzymes and lipid peroxidation in type 2 diabetes mellitus with microvascular complications. The Open Endocrinology Journal2009; 3:12-15. Asian Pacific Journal of Tropical Disease,
[52] [52] Tirgar P, Jadav P, Sheth D, Desai T, Tirgar PR, Jadav PD, and Sheth MDB,Therapeutic role of anti-oxidant properties of Emblicaofficinalis(amla) in streptozotocin induced type 1 diabetic rats. Pharmacologyonline2010; 1:728-743.
[53] [53] ShuklaK, Dikshit P, Tyagi MK, Shukla R,and Gambhir JK, Ameliorative effect of Withaniacoagulans on dyslipidaemia and oxidative stress in nicotinamide-streptozotocin induced diabetes mellitus. Food and Chemical Toxicology, 2012.50: 3595-3599.
[54] [54] Kumar R, Kar B, Dolai N, Bala A, and Haldar PK, Evaluation of antihyperglycaemic andantioxidant properties of StreblusasperLour against streptozotocin–induced diabetes in rats. Asian Pacific Journal of Tropical Disease 2012; 2(2): 139-143.
[55] [55] Singh U, Singh S, and Kochhar A, Therapeutic potential of antidiabeticnutraceuticals.Phytopharmacology, 2012;2(2):144-169.
[56] [56] Kedziora-Kornatowska KZ, Luciak M, and Paszkowski J, Lipid peroxidation and activities of antioxidant enzymes in the diabetic kidney: Effect of treatment with angiotensin convertase inhibitors. International Union of Biochemistry and Molecular Biology Life2000; 49: 303–307.
[57] [57] Onyeka CA, Aligwekwe AU, Nwakanma AA, Bakare AA, and Ofoego UC,Effects of Ethanolic Root Bark Extract of Chrysophyllumalbidumon Serum Superoxide Dismutase, Catalase and Malondialdehyde in Rat. International Journal of PharmaSciences and Research2012; 3(3):347-351.
[58] [58] Rauscher FM, Sanders RA, and Watkins JB,Effects of coenzyme Q10 treatment on antioxidant pathways in normal and streptozotocin-induced diabetic rats. Journal of Biochemistry and Molecular Toxicology2001; 15: 41–46
[59] [59] Kim BJ, Hood BL, Aragon RA, Hardwick JP, Conrads TP, Veenstra TD, Song BJ. Increased oxidation and degradation of cytosolic proteins in alcohol-exposed mouse liver and hepatoma cells. Proteomics2006; 6: 1250-1260.
[60] [60] Onyema OO, Farombi EO, Emerole GO, Ukoha AI andOnyeze GO, Effect ofvitamin E on monosodium glutamate induced hepatotoxicity and oxidative stress in rats.Indian Journal of Biochemistry Biophysics2006; 43: 20-24.
[61] [61] Erejuwa OO, Sulaiman SA, Wahab MS, Sirajudeen KNS, Salleh MS andGurtu S, Effect of Glibenclamide alone versus Glibenclamide and Honey on Oxidative Stress in Pancreas of Streptozotocin-Induced Diabetic Rats. International Journal of Applied Research in Natural Products2011;4(2): 1-10.
[62] [62] Moussa SA. Oxidative Stress in Diabetes Mellitus. Romanian Journal of Biophysics2008; 18(3): 225–236.
[63] [63] Seren S, Leberman R, Bayraktar UD, Health EH, Sahin K, Andic F, andKucuk, O,Lycopene in cancer prevention and treatment. American Journal of Therpeutics 2008; 15:66-81.
[64] [64] Dauqan E,Sani H A, Abdullah A, and Kasim ZM, effect of four different vegetable oils (red palm olein, palm olein, corn oil, coconut oil) on antioxidant enzymes activity of rat liver. Pakistan Journal of Biological Sciences 2011; 14: 399-403.
[65] [65] Bhoomika A D andVaghela NR, Supercritical fluid extraction of lycopene from tomatos by using CO2 as a solvent: A review. Journal of Chemical and Pharmaceutical Research2013; 5(4):188-191
[66] [66] Eteng MU, Ibekwe HA, Essien AD, and Onyeama HP, Effects of Catharanthusroseus on Electrolyte Derangement induced by Chlopropamide (Diabinese)R on Normoglycemic Albino Wistar Rat. Bio- Research2008;6(2): 364-366.
[67] [67] Item JA, Patrick EE, Godwin EE, andIme FA, Effects of Co-administration of Extracts of Vernonia Amygdalina and AzadirachtaIndicaon Serum Electrolyte Profile of Diabetic and non Diabetic Rats. Australian Journal of Basic and Applied Sciences 2009; 3(3):2974-2978.
[68] [68] Tanko Y, Ismail AS,Mohammed KA, Eze ED, Jimoh A, Sada NM, Muhammad A, and Mohammed A,Ameliorative Effects of Magnesium and Copper Sulphateson Blood glucose and Serum Electrolytes Levels in Fructose-induced Diabetic Wistar Rats. Journal of Applied Pharmaceutical Science2013; 3(07): 160-163.
[69] [69] Madsen TE, Muhlestein JB, Carlquist JF, Horne BD, Bair TL, Jackson JD, Lappe, JM, Pearson RR, and Anderson JL, Serum uric acid independently predicts mortality in patients with significant, angiographically defined coronary disease. American Journal of Nephrology2005; 25: 45-49.
[70] [70] Sapna SL, Yogesh S, Amit S, Ekta AA, and Alok ML,Hyperuricemia, High Serum Urea and Hypoproteinemia are the Risk Factor for Diabetes. Asian Journal of Medical Sciences 2009; 1(2): 33-34.
[71] [71] Trujillo, J., Chirino, Y.I, Molina-Jijon, E., Anderica-Romero, A.C., Tapia, E. and PedrazaChaverri, J,Renoprotective effect of the antioxidant curcumin: Recent findings.Redox Biology2013; 1(1):448–456.
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    Eze Ejike Daniel, Aliyu Mohammed, Yusuf Tanko, Abubakar Ahmed. (2015). Effect of Lycopene on Altered Kidney Antioxidant Enzymes Activity and Functions in Streptozotocin-Induced Diabetic Wistar Rats. Cell Biology, 3(1), 1-13. https://doi.org/10.11648/j.cb.20150301.11

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    Eze Ejike Daniel; Aliyu Mohammed; Yusuf Tanko; Abubakar Ahmed. Effect of Lycopene on Altered Kidney Antioxidant Enzymes Activity and Functions in Streptozotocin-Induced Diabetic Wistar Rats. Cell Biol. 2015, 3(1), 1-13. doi: 10.11648/j.cb.20150301.11

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    AMA Style

    Eze Ejike Daniel, Aliyu Mohammed, Yusuf Tanko, Abubakar Ahmed. Effect of Lycopene on Altered Kidney Antioxidant Enzymes Activity and Functions in Streptozotocin-Induced Diabetic Wistar Rats. Cell Biol. 2015;3(1):1-13. doi: 10.11648/j.cb.20150301.11

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  • @article{10.11648/j.cb.20150301.11,
      author = {Eze Ejike Daniel and Aliyu Mohammed and Yusuf Tanko and Abubakar Ahmed},
      title = {Effect of Lycopene on Altered Kidney Antioxidant Enzymes Activity and Functions in Streptozotocin-Induced Diabetic Wistar Rats},
      journal = {Cell Biology},
      volume = {3},
      number = {1},
      pages = {1-13},
      doi = {10.11648/j.cb.20150301.11},
      url = {https://doi.org/10.11648/j.cb.20150301.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20150301.11},
      abstract = {The present study assessed the effects of lycopene on kidney antioxidant enzymes activities and functions in streptozotocin-induced diabetic Wistar rats.Diabetes was induced in animals by single intra-peritoneal injection of streptozotocin. Thereafter the animals were randomly assigned into the following groups: Group I and II (Normal control + olive oil and Diabetic control + olive oil)while Group III to VI were treated with (10, 20 and 40 mg/kg of lycopene and 2 mg/kg glibenclamide) respectively. Alltreatments were givenonce daily orally for four weeks. Results obtained showed that blood glucose was significantly (P < 0.05) reduced. MDAconcentration was reduced in kidney tissue, with increased activities of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase) in diabetic animals administered with lycopene when compared with diabetic control group.There was significant (P < 0.05) increase in the level of serum sodium ion and reduction in serum urea level in diabetic rats treated with lycopene when compared with the diabetic control group. Histological findings showed improved renal architecture as reflected by reduced glomerular and tubular necrosisin all treated groups when compared with control group. It can be concluded that lycopene protects against diabetes-induced kidney damage through elevation of endogenous antioxidant enzymes and improved renal dysfunction in diabetic animals.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Effect of Lycopene on Altered Kidney Antioxidant Enzymes Activity and Functions in Streptozotocin-Induced Diabetic Wistar Rats
    AU  - Eze Ejike Daniel
    AU  - Aliyu Mohammed
    AU  - Yusuf Tanko
    AU  - Abubakar Ahmed
    Y1  - 2015/01/12
    PY  - 2015
    N1  - https://doi.org/10.11648/j.cb.20150301.11
    DO  - 10.11648/j.cb.20150301.11
    T2  - Cell Biology
    JF  - Cell Biology
    JO  - Cell Biology
    SP  - 1
    EP  - 13
    PB  - Science Publishing Group
    SN  - 2330-0183
    UR  - https://doi.org/10.11648/j.cb.20150301.11
    AB  - The present study assessed the effects of lycopene on kidney antioxidant enzymes activities and functions in streptozotocin-induced diabetic Wistar rats.Diabetes was induced in animals by single intra-peritoneal injection of streptozotocin. Thereafter the animals were randomly assigned into the following groups: Group I and II (Normal control + olive oil and Diabetic control + olive oil)while Group III to VI were treated with (10, 20 and 40 mg/kg of lycopene and 2 mg/kg glibenclamide) respectively. Alltreatments were givenonce daily orally for four weeks. Results obtained showed that blood glucose was significantly (P < 0.05) reduced. MDAconcentration was reduced in kidney tissue, with increased activities of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase) in diabetic animals administered with lycopene when compared with diabetic control group.There was significant (P < 0.05) increase in the level of serum sodium ion and reduction in serum urea level in diabetic rats treated with lycopene when compared with the diabetic control group. Histological findings showed improved renal architecture as reflected by reduced glomerular and tubular necrosisin all treated groups when compared with control group. It can be concluded that lycopene protects against diabetes-induced kidney damage through elevation of endogenous antioxidant enzymes and improved renal dysfunction in diabetic animals.
    VL  - 3
    IS  - 1
    ER  - 

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Author Information
  • Department of Physiology, Faculty of Basic Medical Sciences, Bingham University, Karu, Nasarawa State, Nigeria

  • Department of Human Physiology, Faculty of Medicine, Ahmadu Bello University, Zaria, Nigeria

  • Department of Human Physiology, Faculty of Medicine, Ahmadu Bello University, Zaria, Nigeria

  • Department of Pharmacognosy and Drug Development, Ahmadu Bello University, Zaria, Nigeria

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