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Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice

Received: 8 September 2014     Accepted: 20 September 2014     Published: 30 September 2014
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Abstract

Plant phytoestrogens interfere with normal estrogen-regulated functions like steroid synthesis and gonad physiology and morphology. Much evidence has been obtained by using high dose treatments or in vitro exposure to phytoestrogens but little is known about low, dietary level concentrations of these compounds, particularly coumestrol. In order to explore the possible effects on gonads and serum progesterone of oral 10, 20 or 40 μg/Kg body weight dose coumestrol were administered to three experimental groups and compared to a vehicle-only control group (n=5 animals per group) for two weeks and a similar period for treatment recovery. After treatment, testes and blood were obtained and processed for testis and sperm morphology alterations, and steroid hormone evaluation, respectively. Coumestrol treatment induces a significant dose-dependent testis volume decrease and a decrease in 17β hydroxysteroid dehydrogenase activity causing a progesterone increase in response to phytoestrogen concentration. These alterations impair the normal sperm production with an increase in abnormal head and tail shapes. These data strongly suggest a deleterious effect of oral, low concentration phytoestrogen content in adult male diets.

Published in Cell Biology (Volume 2, Issue 4)
DOI 10.11648/j.cb.20140204.12
Page(s) 36-40
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Phytoestrogens, Coumestrol, Testis Alterations, Serum Progesterone, Mice

References
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Cite This Article
  • APA Style

    Hector Serrano, Guillermo Mora-Ramiro, Sheila Peña-Corona, Pablo León-Ortíz, Arturo Salame-Mendez, et al. (2014). Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice. Cell Biology, 2(4), 36-40. https://doi.org/10.11648/j.cb.20140204.12

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    ACS Style

    Hector Serrano; Guillermo Mora-Ramiro; Sheila Peña-Corona; Pablo León-Ortíz; Arturo Salame-Mendez, et al. Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice. Cell Biol. 2014, 2(4), 36-40. doi: 10.11648/j.cb.20140204.12

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    AMA Style

    Hector Serrano, Guillermo Mora-Ramiro, Sheila Peña-Corona, Pablo León-Ortíz, Arturo Salame-Mendez, et al. Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice. Cell Biol. 2014;2(4):36-40. doi: 10.11648/j.cb.20140204.12

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  • @article{10.11648/j.cb.20140204.12,
      author = {Hector Serrano and Guillermo Mora-Ramiro and Sheila Peña-Corona and Pablo León-Ortíz and Arturo Salame-Mendez and Enrique Mendieta-Márques and José Luis Gómez-Olivares and María Dolores García-Suárez},
      title = {Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice},
      journal = {Cell Biology},
      volume = {2},
      number = {4},
      pages = {36-40},
      doi = {10.11648/j.cb.20140204.12},
      url = {https://doi.org/10.11648/j.cb.20140204.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20140204.12},
      abstract = {Plant phytoestrogens interfere with normal estrogen-regulated functions like steroid synthesis and gonad physiology and morphology. Much evidence has been obtained by using high dose treatments or in vitro exposure to phytoestrogens but little is known about low, dietary level concentrations of these compounds, particularly coumestrol. In order to explore the possible effects on gonads and serum progesterone of oral 10, 20 or 40 μg/Kg body weight dose coumestrol were administered to three experimental groups and compared to a vehicle-only control group (n=5 animals per group) for two weeks and a similar period for treatment recovery. After treatment, testes and blood were obtained and processed for testis and  sperm morphology alterations, and steroid hormone evaluation, respectively. Coumestrol treatment induces a significant dose-dependent testis volume decrease and a decrease in 17β hydroxysteroid dehydrogenase activity causing a progesterone increase in response to phytoestrogen concentration. These alterations impair the normal sperm production with an increase in abnormal head and tail shapes. These data strongly suggest a deleterious effect of oral, low concentration phytoestrogen content in adult male diets.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice
    AU  - Hector Serrano
    AU  - Guillermo Mora-Ramiro
    AU  - Sheila Peña-Corona
    AU  - Pablo León-Ortíz
    AU  - Arturo Salame-Mendez
    AU  - Enrique Mendieta-Márques
    AU  - José Luis Gómez-Olivares
    AU  - María Dolores García-Suárez
    Y1  - 2014/09/30
    PY  - 2014
    N1  - https://doi.org/10.11648/j.cb.20140204.12
    DO  - 10.11648/j.cb.20140204.12
    T2  - Cell Biology
    JF  - Cell Biology
    JO  - Cell Biology
    SP  - 36
    EP  - 40
    PB  - Science Publishing Group
    SN  - 2330-0183
    UR  - https://doi.org/10.11648/j.cb.20140204.12
    AB  - Plant phytoestrogens interfere with normal estrogen-regulated functions like steroid synthesis and gonad physiology and morphology. Much evidence has been obtained by using high dose treatments or in vitro exposure to phytoestrogens but little is known about low, dietary level concentrations of these compounds, particularly coumestrol. In order to explore the possible effects on gonads and serum progesterone of oral 10, 20 or 40 μg/Kg body weight dose coumestrol were administered to three experimental groups and compared to a vehicle-only control group (n=5 animals per group) for two weeks and a similar period for treatment recovery. After treatment, testes and blood were obtained and processed for testis and  sperm morphology alterations, and steroid hormone evaluation, respectively. Coumestrol treatment induces a significant dose-dependent testis volume decrease and a decrease in 17β hydroxysteroid dehydrogenase activity causing a progesterone increase in response to phytoestrogen concentration. These alterations impair the normal sperm production with an increase in abnormal head and tail shapes. These data strongly suggest a deleterious effect of oral, low concentration phytoestrogen content in adult male diets.
    VL  - 2
    IS  - 4
    ER  - 

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Author Information
  • Dept. of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico

  • Dept. of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico

  • Dept. of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico

  • Depts. of Biology of Reproduction, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico

  • Dept. of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico

  • Dept. of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico

  • Depts. of Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico

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