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A Multicenter, Randomized Field Trial on the Efficacy and Safety of VEPURED®, A New Vaccine Against Edema Disease in Pigs

Received: 11 December 2018     Accepted: 2 January 2019     Published: 31 January 2019
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Abstract

The aim of this study was to evaluate under field conditions the efficacy and safety of Vepured, a new recombinant vaccine against Edema Disease in pigs. The study was conducted on five commercial farrow-to-finish pig farms, which had historical records of clinical signs and presented F18-positive E.coli producing VT2e. The study was designed as a multicenter, randomized, placebo-controlled, blinded field trial comparing Vepured vaccine to a placebo (phosphate-buffered saline). Animals, at the age of 2-3 days, were administered intramuscularly with 1mL of Vepured (n=945) or with 1mL of phosphate-buffered saline (n=824). After product administration, animals were followed-up until slaughter. During this period, several efficacy and safety parameters were evaluated. On each farm, animals from both groups were held in the same unit and subjected to the same husbandry practices throughout the study. Clinical outbreaks of edema disease were only reported on four farms. On these farms, vaccination with Vepured resulted in a statistically significant reduction in both the mortality rate and the occurrence of clinical signs related to the disease. A statistically significantly higher mean growth performance was also reported in the vaccinated group compared to the placebo group. In the farm without clinical outbreak of edema disease differences were also observed in growth performance in favor of the vaccinated group. No systemic reactions were observed during or immediately after vaccination with Vepured. Only mild transient local reactions, and slight clinically non-relevant temperature increases were reported in some animals. The results obtained in this study demonstrate that vaccination with Vepured is safe and efficacious against naturally occurring edema disease infection.

Published in Animal and Veterinary Sciences (Volume 6, Issue 6)
DOI 10.11648/j.avs.20180606.11
Page(s) 95-101
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Edema Disease, Vaccine, Verotoxin 2e (VT2e), Vepured, Pig

References
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[2] Casanova N. A., Redondo L. M., Dailoff G. C., Arenas D. and Fernández Miyakawa M. E. 2018. Overview of the role of Shiga toxins in porcine edema disease pathogenesis. Toxicon. 15: 148-154.
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[4] Clugston R. E., Nielsen N. O. and Smith D. L. T. 1974. Experimental edema disease of swine (E. coli enterotoxemia) III. Pathology and pathogenesis. Can J Comp Med, 38: 34-43.
[5] MacLeod D. L., Gyles C. L. and Wilcock B. P. 1991. Reproduction of oedema disease of swine with purified Shiga-like toxin-II variant. Vet Pathol, 28: 66–73.
[6] Bosworth B. T., Samuel J. E., Moon H. W., O'brien A. D., Gordon V. M. and Whipp S. C. 1996. Vaccination with genetically modified Shiga-like toxin IIe prevents oedema disease in swine. Infect Immun, 64: 55-60.
[7] Bosworth B. T., Green R. A. and Morrison R. B. 1994. Oedema disease: A search for a genetic link. J Swine Health Prod, 2(3): 19-22.
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[12] Won G., John Hwa L. 2017. Potent immune responses induced by a Salmonella ghost delivery system that expresses the recombinant Stx2eB, FedF, and FedA proteins of the Escherichia coli-producing F18 and Shiga toxin in a murine model and evaluation of its protective effect as a porcine vaccine candidate. Vet Q. 37(1): 81-90.
[13] Toshio S., Takeshi M., Eiji T., Yumiko K., Sou-Ichi M., Ko K., Kazutoshi S. and Takashi H. 2013. Evaluation of Recombinant Forms of the Shiga Toxin Variant Stx2eB Subunit and Non-Toxic Mutant Stx2e as Vaccine Candidates against Porcine Edema Disease. J Vet Med Sci. 75(10): 1309–1315.
[14] Oanh T. K., Nguyen V. K., de Greve H. and Goddeeris B. M. 2012. Protection of piglets against oedema disease by maternal immunization with Stx2e toxoid. Infect Immun, 80: 469–73.
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Cite This Article
  • APA Style

    Eva Perozo, Joaquim Mallorquí, Ainhoa Puig, David Sabaté, Laura Ferrer-Soler, et al. (2019). A Multicenter, Randomized Field Trial on the Efficacy and Safety of VEPURED®, A New Vaccine Against Edema Disease in Pigs. Animal and Veterinary Sciences, 6(6), 95-101. https://doi.org/10.11648/j.avs.20180606.11

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    ACS Style

    Eva Perozo; Joaquim Mallorquí; Ainhoa Puig; David Sabaté; Laura Ferrer-Soler, et al. A Multicenter, Randomized Field Trial on the Efficacy and Safety of VEPURED®, A New Vaccine Against Edema Disease in Pigs. Anim. Vet. Sci. 2019, 6(6), 95-101. doi: 10.11648/j.avs.20180606.11

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    AMA Style

    Eva Perozo, Joaquim Mallorquí, Ainhoa Puig, David Sabaté, Laura Ferrer-Soler, et al. A Multicenter, Randomized Field Trial on the Efficacy and Safety of VEPURED®, A New Vaccine Against Edema Disease in Pigs. Anim Vet Sci. 2019;6(6):95-101. doi: 10.11648/j.avs.20180606.11

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  • @article{10.11648/j.avs.20180606.11,
      author = {Eva Perozo and Joaquim Mallorquí and Ainhoa Puig and David Sabaté and Laura Ferrer-Soler and Ricard March},
      title = {A Multicenter, Randomized Field Trial on the Efficacy and Safety of VEPURED®, A New Vaccine Against Edema Disease in Pigs},
      journal = {Animal and Veterinary Sciences},
      volume = {6},
      number = {6},
      pages = {95-101},
      doi = {10.11648/j.avs.20180606.11},
      url = {https://doi.org/10.11648/j.avs.20180606.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20180606.11},
      abstract = {The aim of this study was to evaluate under field conditions the efficacy and safety of Vepured, a new recombinant vaccine against Edema Disease in pigs. The study was conducted on five commercial farrow-to-finish pig farms, which had historical records of clinical signs and presented F18-positive E.coli producing VT2e. The study was designed as a multicenter, randomized, placebo-controlled, blinded field trial comparing Vepured vaccine to a placebo (phosphate-buffered saline). Animals, at the age of 2-3 days, were administered intramuscularly with 1mL of Vepured (n=945) or with 1mL of phosphate-buffered saline (n=824). After product administration, animals were followed-up until slaughter. During this period, several efficacy and safety parameters were evaluated. On each farm, animals from both groups were held in the same unit and subjected to the same husbandry practices throughout the study. Clinical outbreaks of edema disease were only reported on four farms. On these farms, vaccination with Vepured resulted in a statistically significant reduction in both the mortality rate and the occurrence of clinical signs related to the disease. A statistically significantly higher mean growth performance was also reported in the vaccinated group compared to the placebo group. In the farm without clinical outbreak of edema disease differences were also observed in growth performance in favor of the vaccinated group. No systemic reactions were observed during or immediately after vaccination with Vepured. Only mild transient local reactions, and slight clinically non-relevant temperature increases were reported in some animals. The results obtained in this study demonstrate that vaccination with Vepured is safe and efficacious against naturally occurring edema disease infection.},
     year = {2019}
    }
    

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    AU  - Eva Perozo
    AU  - Joaquim Mallorquí
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    AB  - The aim of this study was to evaluate under field conditions the efficacy and safety of Vepured, a new recombinant vaccine against Edema Disease in pigs. The study was conducted on five commercial farrow-to-finish pig farms, which had historical records of clinical signs and presented F18-positive E.coli producing VT2e. The study was designed as a multicenter, randomized, placebo-controlled, blinded field trial comparing Vepured vaccine to a placebo (phosphate-buffered saline). Animals, at the age of 2-3 days, were administered intramuscularly with 1mL of Vepured (n=945) or with 1mL of phosphate-buffered saline (n=824). After product administration, animals were followed-up until slaughter. During this period, several efficacy and safety parameters were evaluated. On each farm, animals from both groups were held in the same unit and subjected to the same husbandry practices throughout the study. Clinical outbreaks of edema disease were only reported on four farms. On these farms, vaccination with Vepured resulted in a statistically significant reduction in both the mortality rate and the occurrence of clinical signs related to the disease. A statistically significantly higher mean growth performance was also reported in the vaccinated group compared to the placebo group. In the farm without clinical outbreak of edema disease differences were also observed in growth performance in favor of the vaccinated group. No systemic reactions were observed during or immediately after vaccination with Vepured. Only mild transient local reactions, and slight clinically non-relevant temperature increases were reported in some animals. The results obtained in this study demonstrate that vaccination with Vepured is safe and efficacious against naturally occurring edema disease infection.
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Author Information
  • Research and Development Department, HIPRA, Amer, Spain

  • Research and Development Department, HIPRA, Amer, Spain

  • Research and Development Department, HIPRA, Amer, Spain

  • Research and Development Department, HIPRA, Amer, Spain

  • Research and Development Department, HIPRA, Amer, Spain

  • Research and Development Department, HIPRA, Amer, Spain

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