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Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin

Received: 13 December 2015     Accepted: 27 April 2016     Published: 23 May 2016
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Abstract

Simplifying and improving our understanding of the protocols for inducing or synchronizing estrus is important for improving management of dairy cattle. This study evaluated the estrus response and conception rate of crossbred (Holstein Friesian X Zebu) dairy cows (n=75) and heifers (n=47) assigned to a 10-day controlled internal drug release (CIDR) device – prostaglandin F2-alpha (PGF2α) – equine chorionic gonadotrophin (eCG) based estrus synchronization protocol. Animals were assigned to three groups (a) anestrus (n=62), (b) repeat-breeders (n=11) and (c) silent estrus (n=46), according to their reproductive history, per rectal palpation, and progesterone concentrations determined by On-Farm enzyme-linked immunosorbent assays (ELISA) in milk (lactating cows) or serum (dry cows and heifers). For each animal, a CIDR device (containing 1.38g progesterone) was inserted into the vagina and allowed to remain in-situ for 10 days. On Day 8 after device insertion, a dose of 500µg of PGF2α was administered intramuscularly (IM). On Day 10 (device removal), 500 IU of eCG was given IM. Animals were inseminated or mated 48 and 72 hrs post device removal. Pregnancy was assessed 20-24 days post insemination by measurement of progesterone in milk/serum and pregnancy was confirmed by per rectal palpation and/or ultrasonography 44-90 days post insemination. Overall estrus response and conception rates were 97.5% and 78.3%, respectively, with no significant differences in parity, pre-treatment reproductive status and farming system (smallholder vs organized commercial farms). Conception rate after induced/synchronized estrus was 82.9% and 72.1% in cows and heifers, respectively, with an overall calving rate of 94.4%. In conclusion, the present study has shown high estrus response and conception rate in crossbred dairy cattle using this CIDR - PGF2α - eCG based estrus synchronization protocol. Application of this protocol is highly recommended to enhance fertility of dairy cattle in the study area, and this could readily be applied to other regions.

Published in Animal and Veterinary Sciences (Volume 4, Issue 3)
DOI 10.11648/j.avs.20160403.12
Page(s) 39-46
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Cattle, CIDR, eCG, Estrus, Fertility, PGF2α, Synchronization

References
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Cite This Article
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    Alemselam Birhanu Mekonnin, A. Forbes Howie, Christopher R. Harlow, Goitom Gidey, Desalew Tadesse Tegegne, et al. (2016). Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin. Animal and Veterinary Sciences, 4(3), 39-46. https://doi.org/10.11648/j.avs.20160403.12

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    ACS Style

    Alemselam Birhanu Mekonnin; A. Forbes Howie; Christopher R. Harlow; Goitom Gidey; Desalew Tadesse Tegegne, et al. Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin. Anim. Vet. Sci. 2016, 4(3), 39-46. doi: 10.11648/j.avs.20160403.12

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    Alemselam Birhanu Mekonnin, A. Forbes Howie, Christopher R. Harlow, Goitom Gidey, Desalew Tadesse Tegegne, et al. Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin. Anim Vet Sci. 2016;4(3):39-46. doi: 10.11648/j.avs.20160403.12

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  • @article{10.11648/j.avs.20160403.12,
      author = {Alemselam Birhanu Mekonnin and A. Forbes Howie and Christopher R. Harlow and Goitom Gidey and Desalew Tadesse Tegegne and Gidena Desta and Tadesse Gugsa and Berihu Gebrekidan and Gebregiorgis Ashebir and Simon C. Riley},
      title = {Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin},
      journal = {Animal and Veterinary Sciences},
      volume = {4},
      number = {3},
      pages = {39-46},
      doi = {10.11648/j.avs.20160403.12},
      url = {https://doi.org/10.11648/j.avs.20160403.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20160403.12},
      abstract = {Simplifying and improving our understanding of the protocols for inducing or synchronizing estrus is important for improving management of dairy cattle. This study evaluated the estrus response and conception rate of crossbred (Holstein Friesian X Zebu) dairy cows (n=75) and heifers (n=47) assigned to a 10-day controlled internal drug release (CIDR) device – prostaglandin F2-alpha (PGF2α) – equine chorionic gonadotrophin (eCG) based estrus synchronization protocol. Animals were assigned to three groups (a) anestrus (n=62), (b) repeat-breeders (n=11) and (c) silent estrus (n=46), according to their reproductive history, per rectal palpation, and progesterone concentrations determined by On-Farm enzyme-linked immunosorbent assays (ELISA) in milk (lactating cows) or serum (dry cows and heifers). For each animal, a CIDR device (containing 1.38g progesterone) was inserted into the vagina and allowed to remain in-situ for 10 days. On Day 8 after device insertion, a dose of 500µg of PGF2α was administered intramuscularly (IM). On Day 10 (device removal), 500 IU of eCG was given IM. Animals were inseminated or mated 48 and 72 hrs post device removal. Pregnancy was assessed 20-24 days post insemination by measurement of progesterone in milk/serum and pregnancy was confirmed by per rectal palpation and/or ultrasonography 44-90 days post insemination. Overall estrus response and conception rates were 97.5% and 78.3%, respectively, with no significant differences in parity, pre-treatment reproductive status and farming system (smallholder vs organized commercial farms). Conception rate after induced/synchronized estrus was 82.9% and 72.1% in cows and heifers, respectively, with an overall calving rate of 94.4%. In conclusion, the present study has shown high estrus response and conception rate in crossbred dairy cattle using this CIDR - PGF2α - eCG based estrus synchronization protocol. Application of this protocol is highly recommended to enhance fertility of dairy cattle in the study area, and this could readily be applied to other regions.},
     year = {2016}
    }
    

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    T1  - Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin
    AU  - Alemselam Birhanu Mekonnin
    AU  - A. Forbes Howie
    AU  - Christopher R. Harlow
    AU  - Goitom Gidey
    AU  - Desalew Tadesse Tegegne
    AU  - Gidena Desta
    AU  - Tadesse Gugsa
    AU  - Berihu Gebrekidan
    AU  - Gebregiorgis Ashebir
    AU  - Simon C. Riley
    Y1  - 2016/05/23
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    N1  - https://doi.org/10.11648/j.avs.20160403.12
    DO  - 10.11648/j.avs.20160403.12
    T2  - Animal and Veterinary Sciences
    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
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    EP  - 46
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    UR  - https://doi.org/10.11648/j.avs.20160403.12
    AB  - Simplifying and improving our understanding of the protocols for inducing or synchronizing estrus is important for improving management of dairy cattle. This study evaluated the estrus response and conception rate of crossbred (Holstein Friesian X Zebu) dairy cows (n=75) and heifers (n=47) assigned to a 10-day controlled internal drug release (CIDR) device – prostaglandin F2-alpha (PGF2α) – equine chorionic gonadotrophin (eCG) based estrus synchronization protocol. Animals were assigned to three groups (a) anestrus (n=62), (b) repeat-breeders (n=11) and (c) silent estrus (n=46), according to their reproductive history, per rectal palpation, and progesterone concentrations determined by On-Farm enzyme-linked immunosorbent assays (ELISA) in milk (lactating cows) or serum (dry cows and heifers). For each animal, a CIDR device (containing 1.38g progesterone) was inserted into the vagina and allowed to remain in-situ for 10 days. On Day 8 after device insertion, a dose of 500µg of PGF2α was administered intramuscularly (IM). On Day 10 (device removal), 500 IU of eCG was given IM. Animals were inseminated or mated 48 and 72 hrs post device removal. Pregnancy was assessed 20-24 days post insemination by measurement of progesterone in milk/serum and pregnancy was confirmed by per rectal palpation and/or ultrasonography 44-90 days post insemination. Overall estrus response and conception rates were 97.5% and 78.3%, respectively, with no significant differences in parity, pre-treatment reproductive status and farming system (smallholder vs organized commercial farms). Conception rate after induced/synchronized estrus was 82.9% and 72.1% in cows and heifers, respectively, with an overall calving rate of 94.4%. In conclusion, the present study has shown high estrus response and conception rate in crossbred dairy cattle using this CIDR - PGF2α - eCG based estrus synchronization protocol. Application of this protocol is highly recommended to enhance fertility of dairy cattle in the study area, and this could readily be applied to other regions.
    VL  - 4
    IS  - 3
    ER  - 

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Author Information
  • MRC Centre for Reproductive Health, The Queen’s Medical Research Institute College of Medicine and Veterinary Medicine, University of Edinburgh, Edinburgh, United Kingdom

  • MRC Centre for Reproductive Health, The Queen’s Medical Research Institute College of Medicine and Veterinary Medicine, University of Edinburgh, Edinburgh, United Kingdom

  • MRC Centre for Reproductive Health, The Queen’s Medical Research Institute College of Medicine and Veterinary Medicine, University of Edinburgh, Edinburgh, United Kingdom

  • College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia

  • College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia

  • College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia

  • Tigray Regional State Agriculture Bureau, Tigray, Mekelle, Ethiopia

  • College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia

  • College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia

  • MRC Centre for Reproductive Health, The Queen’s Medical Research Institute College of Medicine and Veterinary Medicine, University of Edinburgh, Edinburgh, United Kingdom

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