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Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors

Received: 18 October 2014     Accepted: 3 November 2014     Published: 28 February 2015
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Abstract

Growth and propagation of infectious bursal disease virus (IBDV) in chicken embryo is time consuming and costly, and an appropriate serological test is required to detect and identify IBDV strains, therefore, a suitable cell lines in which different IBDV strains can grow well has been needed. The aim of the present work was to study the growth of different IBDV strains in cell lines from lymphoid leukosis tumors using histopathological staining, indirect immunofluorescence, immunohistochemistry and transmission electron microscopic examination. In conclusion, cell lines from origin of lymphoid leukosis tumors; LSCC-BK3 and LSCC-CU10 are suitable for growth and propagation of different IBDV strains. IBDV strains resulted into histopathological lesions of variable severity differ according to the patho-type of IBDV and time after virus inoculation. Indirect immunofluorescent test could be used for detection and differentiation of IBDV strains inoculated into cell lines from origin of lymphoid leukosis tumors. Trials of immunohistochemistry technique for detection of different IBDV strains in cell lines, were unsuccessful. Using transmission electron microscopy, IBDV particles could be detected in all infected cell lines.

Published in Animal and Veterinary Sciences (Volume 3, Issue 2)
DOI 10.11648/j.avs.20150302.13
Page(s) 46-50
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Infectious Bursal Disease Virus, Cell Lines, Histopathological Staining, Immunohistochemistry, Immunofluorescence

References
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Cite This Article
  • APA Style

    Ahmed Hassan, Mostafa Shahata, Elrefaie Refaie, Ragab Ibrahim, Jun Sasaki, et al. (2015). Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors. Animal and Veterinary Sciences, 3(2), 46-50. https://doi.org/10.11648/j.avs.20150302.13

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    ACS Style

    Ahmed Hassan; Mostafa Shahata; Elrefaie Refaie; Ragab Ibrahim; Jun Sasaki, et al. Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors. Anim. Vet. Sci. 2015, 3(2), 46-50. doi: 10.11648/j.avs.20150302.13

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    AMA Style

    Ahmed Hassan, Mostafa Shahata, Elrefaie Refaie, Ragab Ibrahim, Jun Sasaki, et al. Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors. Anim Vet Sci. 2015;3(2):46-50. doi: 10.11648/j.avs.20150302.13

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  • @article{10.11648/j.avs.20150302.13,
      author = {Ahmed Hassan and Mostafa Shahata and Elrefaie Refaie and Ragab Ibrahim and Jun Sasaki and Masanubu Goryo},
      title = {Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors},
      journal = {Animal and Veterinary Sciences},
      volume = {3},
      number = {2},
      pages = {46-50},
      doi = {10.11648/j.avs.20150302.13},
      url = {https://doi.org/10.11648/j.avs.20150302.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20150302.13},
      abstract = {Growth and propagation of infectious bursal disease virus (IBDV) in chicken embryo is time consuming and costly, and an appropriate serological test is required to detect and identify IBDV strains, therefore, a suitable cell lines in which different IBDV strains can grow well has been needed. The aim of the present work was to study the growth of different IBDV strains in cell lines from lymphoid leukosis tumors using histopathological staining, indirect immunofluorescence, immunohistochemistry and transmission electron microscopic examination. In conclusion, cell lines from origin of lymphoid leukosis tumors; LSCC-BK3 and LSCC-CU10 are suitable for growth and propagation of different IBDV strains. IBDV strains resulted into histopathological lesions of variable severity differ according to the patho-type of IBDV and time after virus inoculation. Indirect immunofluorescent test could be used for detection and differentiation of IBDV strains inoculated into cell lines from origin of lymphoid leukosis tumors. Trials of immunohistochemistry technique for detection of different IBDV strains in cell lines, were unsuccessful. Using transmission electron microscopy, IBDV particles could be detected in all infected cell lines.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors
    AU  - Ahmed Hassan
    AU  - Mostafa Shahata
    AU  - Elrefaie Refaie
    AU  - Ragab Ibrahim
    AU  - Jun Sasaki
    AU  - Masanubu Goryo
    Y1  - 2015/02/28
    PY  - 2015
    N1  - https://doi.org/10.11648/j.avs.20150302.13
    DO  - 10.11648/j.avs.20150302.13
    T2  - Animal and Veterinary Sciences
    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
    SP  - 46
    EP  - 50
    PB  - Science Publishing Group
    SN  - 2328-5850
    UR  - https://doi.org/10.11648/j.avs.20150302.13
    AB  - Growth and propagation of infectious bursal disease virus (IBDV) in chicken embryo is time consuming and costly, and an appropriate serological test is required to detect and identify IBDV strains, therefore, a suitable cell lines in which different IBDV strains can grow well has been needed. The aim of the present work was to study the growth of different IBDV strains in cell lines from lymphoid leukosis tumors using histopathological staining, indirect immunofluorescence, immunohistochemistry and transmission electron microscopic examination. In conclusion, cell lines from origin of lymphoid leukosis tumors; LSCC-BK3 and LSCC-CU10 are suitable for growth and propagation of different IBDV strains. IBDV strains resulted into histopathological lesions of variable severity differ according to the patho-type of IBDV and time after virus inoculation. Indirect immunofluorescent test could be used for detection and differentiation of IBDV strains inoculated into cell lines from origin of lymphoid leukosis tumors. Trials of immunohistochemistry technique for detection of different IBDV strains in cell lines, were unsuccessful. Using transmission electron microscopy, IBDV particles could be detected in all infected cell lines.
    VL  - 3
    IS  - 2
    ER  - 

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Author Information
  • Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, 3-18-8, Ueda, Morioka, 020-8550, Japan

  • Department of Poultry diseases, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt

  • Department of Poultry diseases, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt

  • Department of Poultry diseases, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt

  • Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, 3-18-8, Ueda, Morioka, 020-8550, Japan

  • Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, 3-18-8, Ueda, Morioka, 020-8550, Japan

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