| Peer-Reviewed

In Vitro Culture of Turkish Origanum sipyleum L.

Received: 20 April 2017     Accepted: 21 April 2017     Published: 17 July 2017
Views:       Downloads:
Abstract

Origanum sipyleum L. is a species that is endemic to Turkey with known medicinal and aromatic properties and with a long historical use in Turkish folk medicine. Micropropagation serves as one possible method to clone and protect O. sipyleum. In this study, nodes, which served as the explants, were cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of BA (0.1, 0.5, 1.0 mgL-1) and GA3 (0.1, 0.2 mgL-1). Shoots were transferred to MS medium containing different concentrations of IBA (0, 0.5, 1, 1.5, 2, 2.5, 3 mgL-1) for root induction. Shoot regeneration, rooting, survival and acclimatization were possible. As many as 85% of nodes formed an average of 6 shoots/node on MS medium supplemented with 0.5 mgL-1 BA and 0.2 mgL-1 GA3. Rooting was most successful in 62.5% of shoots on MS medium with 1.5 or 2.5 mgL-1 IBA. Plantlets derived from all IBA combinations were transferred to autoclaved peat and perlite (1:1, v/v) and acclimatized in a greenhouse, with 79.1% survival. This protocol represents the first comprehensive in vitro regeneration protocol for endemic (Turkish) O. sipyleum.

Published in American Journal of Plant Biology (Volume 2, Issue 3-1)

This article belongs to the Special Issue Plant Molecular Biology and Biotechnology

DOI 10.11648/j.ajpb.s.2017020301.16
Page(s) 32-36
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

BA, GA3, IBA, MS Medium, Tissue Culture

References
[1] B. T. Ünal, A. Guvensen, A. E. Dereboylu, M. Öztürk, ''Variations in the proline and total protein contents in Origanum sipyleum L. from different altitudes of Spil mountain Turkey''. Pak. J. Bot., 45, 571-576, 2013.
[2] M. Kürşat, I. Emre, O. Yilmaz, P. Erecevit, ''Antioxidant and antimicrobial activity in the seeds of Origanum vulgare L. subsp. gracile (C. Koch) Ietswaart and Origanum acutidens (Hand.-Mazz.) Ietswaart from Turkey'', Grasas y acites, 62 (4), 410-417, 2011.
[3] T. Aşkun, G. Tümen, F. Satil, O. Yalçin, S. Modanlioğlu, ''Antimycobacterial activity some different Lamiaceae plant extracts containing flavonoids and other phenolic compounds,'' (In: Cardona PJ), editors Understanding Tuberculosis - New Approaches to Fighting Against Drug Resistance, InTech, Rijeka, Croatia, 309-336, 2012.
[4] M. U. Yildirim, ''Micropropagation of Origanum acutidens (Hand.-Mazz.) Ietswaart using stem node explants,'' Scientific World J., 1-3, 2013.
[5] M. Skoula, P. Gotsiou, G. Naxakis, C. B. Johnson, ''A chemosystematic investigation on the mono and sesquiterpenoids in the genus Origanum (Labiatae),'' Phytochemistry. 52 (4), 649-657, 1999.
[6] S. Zein, S. Awada, S. Rachidi, A. Hajj, E. Krivoruschko, H. Kanaan, ''Chemical analysis of essential oil from Lebanese wild and cultivated Origanum syriacum l. (Lamiaceae) before and after flowering,'' J. Med. Plants Res., 5, 379-387, 2011.
[7] K. H. C. Başer, T. Özek, M. Kürkçüoğlu, ''Composition of the essential oil of Origanum sipyleum L. of Turkish origin,'' J. Essential Oil Res., 4, 139-142, 1992.
[8] M. Shahwan, T. Al-Qirim, A. Badder, '' Short-term feeding effect of Origanum syriacum crude extract on immobilization stress induced antioxidant defence changes in rat,'' J. Biol. Sci., 12 (7), 421-425, 2012.
[9] P. H. Davis, ''Flora of Turkey and the East Aegean Islands (7)''. Edinburgh: Edinburgh University Press., 400-461, 1982.
[10] A. E. Oluk and A. Çakir, ''Micropropagation of Origanum sipyleum L., an endemic medicinal herb of Turkey,'' African J. Biotechnol., 8 (21), 5769-5772, 2009.
[11] T. Murashige, F. Skoog, ''A revised medium for rapid growth and bio assays with tobacco tissue cultures,'' Physiol. Plant., 15 (3), 473-497, 1962.
[12] D. Özkum, ''In vitro shoot regeneration of oregano (Origanum minutiflorum O. Schwarz & Davis),'' Hacettepe J. Biol. Chem., 35 (2), 97-100, 2007.
[13] O. L. Gamborg, R. Miller, K. Ojima, ''Nutrient requirements of suspension cultures of soybean root cells,'' Exp. Cell Res. 50 (1), 151-158, 1968.
[14] G. N. Iconomou-Petrovich, I. Nianiou-Obeidat, ''Micropropagation of Origanum vulgare subsp. hirtum (Mt. Taygetos)''. In: Tsekos I, Moustakas M, (editors) Progress in Botanical Research, Springer, Berlin, 509-512, 1998.
[15] D. Leelavathi and N. Kuppan, ''Callus induction and regeneration of multiple shoots from in vitro apical bud explant of Origanum vulgare, an important medicinal plant,'' Int. J. Res. Pharm. Chem., 898-903, 2013.
[16] I. Morone-Fortunato, P. Avato, ''Plant development and synthesis of essential oils in micropropagated and mycorrhiza inoculated plants of Origanum vulgare L. ssp. hirtum,'' Plant Cell Tiss. Organ Cult., 93 (2), 139-149, 2008.
[17] V. Lattanzio, A. Cardinali, C. Ruta, I. M. Fortunato, V. M. Lattanzi, V. Linsalata, N. Cicco, ''Relationship of secondary metabolism to growth in oregano (Origanum vulgare L.) shoot cultures under nutritional stress,'' Env. Exp. Bot., 65 (1), 54-62, 2009.
[18] K. Ueno and K. Shetty, ''Prevention of hyperhydricity in oregano shoot cultures is sustained through multiple subcultures by selected polysaccharide-producing soil bacteria without re-inoculation,'' Applied Microbiol. Biotechnol., 50 (1), 119-124, 1998.
[19] M. E. Goleniowski, C. Flamarique, P. Bima, ''Micropropagation of oregano (Origanum vulgare × applii) from meristem tips,'' In Vitro Cell. Dev. Biol. Plant. 39: 125-128, 2003.
[20] O. Socorro, I. Tarrega, F. Rivas, ''Essential oils from wild and micropropagated plants of Origanum bastetanum,'' Phytochemistry, 48 (8), 1347-1349, 1998.
[21] R. M. Arafeh, S. M. Mahmoud, A. R. Shibli, ''In vitro seed propagation of wild Syrian marjoram (Origanum syriacum),'' Adv. Hort. Sci., 17 (4), 241-244, 2003.
[22] K. Shetty, O. F. Curtis, R. E. Levin, Witkowsky, W. Ang, ''Prevention of vitrification associated with in vitro shoot culture of oregano (Origanum vulgare) by Pseudomonas spp.,'' J. Plant Physiol., 147 (3), 447-451, 1995.
[23] R. M. Arafeh, A. R. Shibli, M. Al-Mahmoud, M. A. Shatnawi, ''Callusing, cell suspension culture and secondary metabolites production in Persian oregano (Origanum vulgare L.) and Arabian oregano (O. syriacum L.),'' Jordan J. Agric. Sci., 2 (3), 274-281, 2006.
Cite This Article
  • APA Style

    Başar Sevindik, Tolga İzgü, Özhan Şimşek, Mehmet Tütüncü, Pembe Çürük, et al. (2017). In Vitro Culture of Turkish Origanum sipyleum L.. American Journal of Plant Biology, 2(3-1), 32-36. https://doi.org/10.11648/j.ajpb.s.2017020301.16

    Copy | Download

    ACS Style

    Başar Sevindik; Tolga İzgü; Özhan Şimşek; Mehmet Tütüncü; Pembe Çürük, et al. In Vitro Culture of Turkish Origanum sipyleum L.. Am. J. Plant Biol. 2017, 2(3-1), 32-36. doi: 10.11648/j.ajpb.s.2017020301.16

    Copy | Download

    AMA Style

    Başar Sevindik, Tolga İzgü, Özhan Şimşek, Mehmet Tütüncü, Pembe Çürük, et al. In Vitro Culture of Turkish Origanum sipyleum L.. Am J Plant Biol. 2017;2(3-1):32-36. doi: 10.11648/j.ajpb.s.2017020301.16

    Copy | Download

  • @article{10.11648/j.ajpb.s.2017020301.16,
      author = {Başar Sevindik and Tolga İzgü and Özhan Şimşek and Mehmet Tütüncü and Pembe Çürük and Özer Yılmaz and Gönül Kaynak and Yıldız Aka Kaçar and Jaime A. Teixeira da Silva and Yeşim Yalçın Mendi},
      title = {In Vitro Culture of Turkish Origanum sipyleum L.},
      journal = {American Journal of Plant Biology},
      volume = {2},
      number = {3-1},
      pages = {32-36},
      doi = {10.11648/j.ajpb.s.2017020301.16},
      url = {https://doi.org/10.11648/j.ajpb.s.2017020301.16},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.s.2017020301.16},
      abstract = {Origanum sipyleum L. is a species that is endemic to Turkey with known medicinal and aromatic properties and with a long historical use in Turkish folk medicine. Micropropagation serves as one possible method to clone and protect O. sipyleum. In this study, nodes, which served as the explants, were cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of BA (0.1, 0.5, 1.0 mgL-1) and GA3 (0.1, 0.2 mgL-1). Shoots were transferred to MS medium containing different concentrations of IBA (0, 0.5, 1, 1.5, 2, 2.5, 3 mgL-1) for root induction. Shoot regeneration, rooting, survival and acclimatization were possible. As many as 85% of nodes formed an average of 6 shoots/node on MS medium supplemented with 0.5 mgL-1 BA and 0.2 mgL-1 GA3. Rooting was most successful in 62.5% of shoots on MS medium with 1.5 or 2.5 mgL-1 IBA. Plantlets derived from all IBA combinations were transferred to autoclaved peat and perlite (1:1, v/v) and acclimatized in a greenhouse, with 79.1% survival. This protocol represents the first comprehensive in vitro regeneration protocol for endemic (Turkish) O. sipyleum.},
     year = {2017}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - In Vitro Culture of Turkish Origanum sipyleum L.
    AU  - Başar Sevindik
    AU  - Tolga İzgü
    AU  - Özhan Şimşek
    AU  - Mehmet Tütüncü
    AU  - Pembe Çürük
    AU  - Özer Yılmaz
    AU  - Gönül Kaynak
    AU  - Yıldız Aka Kaçar
    AU  - Jaime A. Teixeira da Silva
    AU  - Yeşim Yalçın Mendi
    Y1  - 2017/07/17
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ajpb.s.2017020301.16
    DO  - 10.11648/j.ajpb.s.2017020301.16
    T2  - American Journal of Plant Biology
    JF  - American Journal of Plant Biology
    JO  - American Journal of Plant Biology
    SP  - 32
    EP  - 36
    PB  - Science Publishing Group
    SN  - 2578-8337
    UR  - https://doi.org/10.11648/j.ajpb.s.2017020301.16
    AB  - Origanum sipyleum L. is a species that is endemic to Turkey with known medicinal and aromatic properties and with a long historical use in Turkish folk medicine. Micropropagation serves as one possible method to clone and protect O. sipyleum. In this study, nodes, which served as the explants, were cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of BA (0.1, 0.5, 1.0 mgL-1) and GA3 (0.1, 0.2 mgL-1). Shoots were transferred to MS medium containing different concentrations of IBA (0, 0.5, 1, 1.5, 2, 2.5, 3 mgL-1) for root induction. Shoot regeneration, rooting, survival and acclimatization were possible. As many as 85% of nodes formed an average of 6 shoots/node on MS medium supplemented with 0.5 mgL-1 BA and 0.2 mgL-1 GA3. Rooting was most successful in 62.5% of shoots on MS medium with 1.5 or 2.5 mgL-1 IBA. Plantlets derived from all IBA combinations were transferred to autoclaved peat and perlite (1:1, v/v) and acclimatized in a greenhouse, with 79.1% survival. This protocol represents the first comprehensive in vitro regeneration protocol for endemic (Turkish) O. sipyleum.
    VL  - 2
    IS  - 3-1
    ER  - 

    Copy | Download

Author Information
  • Department of Horticulture, Faculty of Agriculture, University of Ondokuz May?s Atakum, Samsun, Turkey

  • Miki-cho Post Office, Ikenobe, Kagawa-ken, Japan

  • Sections