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Cloning and Expression Analysis of GmCYP78A5 Promoter

Received: 21 April 2019     Accepted: 17 June 2019     Published: 4 July 2019
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Abstract

CYP78A5 promoter was isolated from soybean (Glycine max L. Merrill) plant by using PCR technology. DNA sequence alignment indicated that the amplified fragment (1650bp) was 99.21% homologous to the correspondent regions of the reported sequences. Bioinformatics analysis showed that the GmCYP78A5 promoter contains a lot of inducible or tissue-specific expression elements. RT-PCR results indicated that the gene GmCYP78A5 highly expressed in immature seed, weekly expressed in stem of soybean, but no expressed in root, leaf and flower. To further study the tissue expression patterns of GmCYP78A5 gene, the promoter of the gene GmCYP78A5 was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into tobacco (Nicotiana tabacum) by Agrobacterium-meditated method. The expression of the GUS gene in the transgenic tobacco plants indicated that the GmCYP78A5 promoter could drive the GUS reporter gene to express highly in the leaf, stem, sepal, pedicel, seeds of the transgenic tobacco plants, demonstrating that the expression patterns of the GmCYP78A5 promoters in soybean and tobacco were inconsistent.

Published in American Journal of Plant Biology (Volume 4, Issue 1)
DOI 10.11648/j.ajpb.20190401.12
Page(s) 7-11
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Soybean, GmCYP78A5 Promoter, Tissue Specific Expression, GUS Assay

References
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[2] Anastasiou E, Kenz S, Gerstung M, MacLean D, Timmer J, Fleck C, and Lenhard M (2007). Control of Plant Organ Size by KLUH/CYP78A5-Dependent Intercellular Signaling. Developmental Cell, 13: 843-856.
[3] Eriksson S, Stransfeld L, Adamski N M, Breuninger H, Lenhard M (2010). Local maternal control of seed size by KLUH/CYP78A5-dependent growth signaling. Curr Biol, 20 (6):527-32.
[4] Zondlo S C and Irish V F (1999). CYP78A5 encodes a cyptochrome P450 that marks the shoot apical meristem boundary in Arabidopsis. Plant J, 19 (3): 259-268.
[5] Anastasiou E, and Lenhard M (2007). Growing up to one's standard. Curr. Opin. Plant Biol, 10 (1): 63-69.
[6] Adamski N M, Anastasiou E, Eriksson S, O'Neill C M, and Lenhard M (2009). Local maternal control of seed size by KLUH/CYP78A5-dependent growth signaling. Proc. Natl. Acad. Sci. USA, 106 (47): 20115-20120.
[7] Zhong M, Huang G. T, Bai L. P, Zhang L, Ma H, Zhang L. J, and Guo Z. H (2011). New strategy to reconstruct Agrobacterium-mediated plant transgenic expression vectors. Acta Agriculturae Boreali Sinica, 26 (1): 41-46.
[8] Wei J, Mao W H., Lin Y J, and Chen H (2012). Isolation and characterization of a novel rice constitutive promoter. Journal of Huazhong Agricultural University, 31 (2): 139-146.
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[10] Zhao SY, Wu Y R, and Xia G. M (2002). Introduction of a simple and effective method for plant total RNA isolation. Hereditas, 24 (3): 337-338.
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[14] Jefferson R A (1987). Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol. Biol. Rep, 5: 384-405.
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Cite This Article
  • APA Style

    Xiaofeng Chen, Qiuli Du, Chunmei Zhao, Zhaoyong Lv, Ren-Gao Xue. (2019). Cloning and Expression Analysis of GmCYP78A5 Promoter. American Journal of Plant Biology, 4(1), 7-11. https://doi.org/10.11648/j.ajpb.20190401.12

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    ACS Style

    Xiaofeng Chen; Qiuli Du; Chunmei Zhao; Zhaoyong Lv; Ren-Gao Xue. Cloning and Expression Analysis of GmCYP78A5 Promoter. Am. J. Plant Biol. 2019, 4(1), 7-11. doi: 10.11648/j.ajpb.20190401.12

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    AMA Style

    Xiaofeng Chen, Qiuli Du, Chunmei Zhao, Zhaoyong Lv, Ren-Gao Xue. Cloning and Expression Analysis of GmCYP78A5 Promoter. Am J Plant Biol. 2019;4(1):7-11. doi: 10.11648/j.ajpb.20190401.12

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  • @article{10.11648/j.ajpb.20190401.12,
      author = {Xiaofeng Chen and Qiuli Du and Chunmei Zhao and Zhaoyong Lv and Ren-Gao Xue},
      title = {Cloning and Expression Analysis of GmCYP78A5 Promoter},
      journal = {American Journal of Plant Biology},
      volume = {4},
      number = {1},
      pages = {7-11},
      doi = {10.11648/j.ajpb.20190401.12},
      url = {https://doi.org/10.11648/j.ajpb.20190401.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.20190401.12},
      abstract = {CYP78A5 promoter was isolated from soybean (Glycine max L. Merrill) plant by using PCR technology. DNA sequence alignment indicated that the amplified fragment (1650bp) was 99.21% homologous to the correspondent regions of the reported sequences. Bioinformatics analysis showed that the GmCYP78A5 promoter contains a lot of inducible or tissue-specific expression elements. RT-PCR results indicated that the gene GmCYP78A5 highly expressed in immature seed, weekly expressed in stem of soybean, but no expressed in root, leaf and flower. To further study the tissue expression patterns of GmCYP78A5 gene, the promoter of the gene GmCYP78A5 was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into tobacco (Nicotiana tabacum) by Agrobacterium-meditated method. The expression of the GUS gene in the transgenic tobacco plants indicated that the GmCYP78A5 promoter could drive the GUS reporter gene to express highly in the leaf, stem, sepal, pedicel, seeds of the transgenic tobacco plants, demonstrating that the expression patterns of the GmCYP78A5 promoters in soybean and tobacco were inconsistent.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Cloning and Expression Analysis of GmCYP78A5 Promoter
    AU  - Xiaofeng Chen
    AU  - Qiuli Du
    AU  - Chunmei Zhao
    AU  - Zhaoyong Lv
    AU  - Ren-Gao Xue
    Y1  - 2019/07/04
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ajpb.20190401.12
    DO  - 10.11648/j.ajpb.20190401.12
    T2  - American Journal of Plant Biology
    JF  - American Journal of Plant Biology
    JO  - American Journal of Plant Biology
    SP  - 7
    EP  - 11
    PB  - Science Publishing Group
    SN  - 2578-8337
    UR  - https://doi.org/10.11648/j.ajpb.20190401.12
    AB  - CYP78A5 promoter was isolated from soybean (Glycine max L. Merrill) plant by using PCR technology. DNA sequence alignment indicated that the amplified fragment (1650bp) was 99.21% homologous to the correspondent regions of the reported sequences. Bioinformatics analysis showed that the GmCYP78A5 promoter contains a lot of inducible or tissue-specific expression elements. RT-PCR results indicated that the gene GmCYP78A5 highly expressed in immature seed, weekly expressed in stem of soybean, but no expressed in root, leaf and flower. To further study the tissue expression patterns of GmCYP78A5 gene, the promoter of the gene GmCYP78A5 was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into tobacco (Nicotiana tabacum) by Agrobacterium-meditated method. The expression of the GUS gene in the transgenic tobacco plants indicated that the GmCYP78A5 promoter could drive the GUS reporter gene to express highly in the leaf, stem, sepal, pedicel, seeds of the transgenic tobacco plants, demonstrating that the expression patterns of the GmCYP78A5 promoters in soybean and tobacco were inconsistent.
    VL  - 4
    IS  - 1
    ER  - 

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Author Information
  • College of Life Sciences, Qingdao Agricultural University, Qingdao, China

  • Quancheng College, University of Jinan, Penglai, China

  • College of Life Sciences, Qingdao Agricultural University, Qingdao, China

  • College of Life Sciences, Qingdao Agricultural University, Qingdao, China

  • College of Life Sciences, Qingdao Agricultural University, Qingdao, China

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