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Isolation of Fungal Pathogens of Stored Maize, Zea mays L., in Seka Chokersa District of Jimma Zone, Ethiopia

Received: 1 April 2021     Accepted: 19 May 2021     Published: 27 May 2021
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Abstract

Maize, the most common stable food in Africa, is attacked by different pests during its production and post-harvest. Fungi are usually responsible for the deterioration of maize seedlings and grains right in the field or in storage. Storage fungi usually invade grain or seed during storage and are generally not present in large quantities before harvest in the field. They cause quality loss through their production of toxic byproducts which has high risk for human and animal health. The most common storage fungi are species of Aspergillus and Penicillium. This short study was conducted with an objective of isolating and identifying the major fungi genera associated with stored maize grain in Seka Chokersa district of Jimma Zone in 2017. Samples were taken from farmers traditional storage containers. For isolation, the fungi were grown on PDA medium and identified morphologically using a dissecting microscope. Accordingly, common stored cereal grain fungi genera Aspergillus, Fusarium and Penicillium were morphologically identified off the samples collected to their genus level. Among these fungi, Aspergillus spp. was identified with high frequency (86.6%) followed by Fusarium spp. (53.3%) and Penicillium spp. (40%). Aspergillus, Fusarium and Penicillium are among the top fungi genera deteriorating stored cereal grain which have high health risk for both human and animal.

Published in American Journal of Life Sciences (Volume 9, Issue 3)
DOI 10.11648/j.ajls.20210903.11
Page(s) 42-44
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Stored Cereal Grain, Fungal Pathogen, Fungi Genera, Aflatoxin

References
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[2] CSA (Central Statistical Agency) (2012). The Federal Democratic Republic of Ethiopia Agricultural Sample Survey 2011 / 2012. Volume I, Report on Area andF Production of Major Crops (Private Peasant Holdings, Meher Season).
[3] Tsedaley B and Adugna G (2016). Detection of fungi infecting maize (Zea mays L.) seeds in different storages around Jimma, South-Western Ethiopia. Journal of Plant Pathology & Microbiology, 7 (3), p. 338.
[4] Demissie G, Teshome A, Abakemal D, Tadesse A (2008). Cooking oils and “Triplex” in the control of Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) in farm-sored maize. Journal of Sored Products Research, 44 (2), pp. 173-178.
[5] Uzma S and Shahida A (2007). The screening of seven medicinal plants for artificial activity against seed borne fungi of maize seeds. Pakistan Journal of Botany, 39 (1): 285-292.
[6] Castlellarie C, Marcos Valle F, Mutti J, Cardoso L and Bartosik R (2010). Toxigenic fungi in corn (maize) stored in hermetic plastic bags. Julius-Kuhn-Archiv, (425), pp. 501-504.
[7] Richard JL (2007). Some major mycotoxins and their mycotoxicoses: An overview. Int J Food Microbial 119: 3-10.
[8] Charity A., Amienyo J and Dauda T (2010). Effect of relative humidity on spore determination and growth of Aspergillus flavus. Nigerian Journal of Botany, 23 (1): 1-6.
[9] Suleiman MN and Omafe OM (2013). Activity of three medicinal plants on fungi isolated from stored maize seeds (Zea mays L.). Global Journal of Medicinal Plant Research, 1 (1), pp. 77-81.
[10] Sode OJ, Mazoud F and Troude F (1995). Economics of grain storage. In: Jayas DS, White NDG, White WE. (eds.). Stored-grain Ecosystems. Marcel Dekker. pp. 101-122.
[11] Ominski KH, Marquardt RR, Sinha RN and Abramson D (1994). Ecological aspects of growth and mycotoxin production by storage fungi. Mycotoxins in grains. Compounds other than Aflatoxin. Eagen Press, USA. pp. 287-305.
[12] Udoh JM, Cardwell KF and IkotunT (2000). Storage structures and aflatoxin content of maize in five agro-ecological zones of Nigeria. Journal of Stored Products Research 36 (2), 187-201.
[13] Marasas W, Burgess LW, Anelich RY, Lamprecht SC van Schalkwyk DJ (1988). Survey of Fusarium species associated with plant debris in South African soils. South African Journal of Botany, 54 (1), pp. 63-71.
[14] Hassan FF, Al-Jibouri MH and Hashim AKJ (2014). Isolation and Identification of Fungal Propagation in Stored Maize and detection of aflatoxin B1 Using TLC and ELISA Technique. Iraqi Journal of Science, 55 (2B), pp. 634-642.
[15] Dudoiu R, Cristea S, Lupo C, Popa D and Opera M (2016). Micoflora associated with Maize grains during storage period. AgroLife Scientific Journal, 5 (1), pp. 63-68.
[16] Dubale B, Solomon A, Geremew B, Sethumadhava RG and Waktole S (2014). Mycoflora of grain Maize (Zea mays L.) stored in traditional storage containers (Gombisa and Sacks) in selected woredas of Jimma zone, Ethiopia. African journal of food, agriculture, nutrition and development, 14 (2).
[17] Garcia-Diaz M, Gil-Serna J, Vazquez C, Botia MN and Patino B (2020). A Comprehensive Study on the Occurrence of Mycotoxins and Their Producing Fungi during the Maize Production Cycle in Spain. Micoorganisms, 8 (1), p. 141.
[18] Adetunji, M, Atanda O., Ezekiel CN, Sulyok M, Warth B, Beltrán E, Krska R, Obadina O, Bakare A. and Chilaka CA (2014). Fungal and bacterial metabolites of stored maize (Zea mays, L.) from five agro-ecological zones of Nigeria. Mycotoxin research, 30 (2), pp. 89-102.
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  • APA Style

    Garuma Nemera Roge. (2021). Isolation of Fungal Pathogens of Stored Maize, Zea mays L., in Seka Chokersa District of Jimma Zone, Ethiopia. American Journal of Life Sciences, 9(3), 42-44. https://doi.org/10.11648/j.ajls.20210903.11

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    ACS Style

    Garuma Nemera Roge. Isolation of Fungal Pathogens of Stored Maize, Zea mays L., in Seka Chokersa District of Jimma Zone, Ethiopia. Am. J. Life Sci. 2021, 9(3), 42-44. doi: 10.11648/j.ajls.20210903.11

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    AMA Style

    Garuma Nemera Roge. Isolation of Fungal Pathogens of Stored Maize, Zea mays L., in Seka Chokersa District of Jimma Zone, Ethiopia. Am J Life Sci. 2021;9(3):42-44. doi: 10.11648/j.ajls.20210903.11

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  • @article{10.11648/j.ajls.20210903.11,
      author = {Garuma Nemera Roge},
      title = {Isolation of Fungal Pathogens of Stored Maize, Zea mays L., in Seka Chokersa District of Jimma Zone, Ethiopia},
      journal = {American Journal of Life Sciences},
      volume = {9},
      number = {3},
      pages = {42-44},
      doi = {10.11648/j.ajls.20210903.11},
      url = {https://doi.org/10.11648/j.ajls.20210903.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20210903.11},
      abstract = {Maize, the most common stable food in Africa, is attacked by different pests during its production and post-harvest. Fungi are usually responsible for the deterioration of maize seedlings and grains right in the field or in storage. Storage fungi usually invade grain or seed during storage and are generally not present in large quantities before harvest in the field. They cause quality loss through their production of toxic byproducts which has high risk for human and animal health. The most common storage fungi are species of Aspergillus and Penicillium. This short study was conducted with an objective of isolating and identifying the major fungi genera associated with stored maize grain in Seka Chokersa district of Jimma Zone in 2017. Samples were taken from farmers traditional storage containers. For isolation, the fungi were grown on PDA medium and identified morphologically using a dissecting microscope. Accordingly, common stored cereal grain fungi genera Aspergillus, Fusarium and Penicillium were morphologically identified off the samples collected to their genus level. Among these fungi, Aspergillus spp. was identified with high frequency (86.6%) followed by Fusarium spp. (53.3%) and Penicillium spp. (40%). Aspergillus, Fusarium and Penicillium are among the top fungi genera deteriorating stored cereal grain which have high health risk for both human and animal.},
     year = {2021}
    }
    

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    T1  - Isolation of Fungal Pathogens of Stored Maize, Zea mays L., in Seka Chokersa District of Jimma Zone, Ethiopia
    AU  - Garuma Nemera Roge
    Y1  - 2021/05/27
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    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
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    AB  - Maize, the most common stable food in Africa, is attacked by different pests during its production and post-harvest. Fungi are usually responsible for the deterioration of maize seedlings and grains right in the field or in storage. Storage fungi usually invade grain or seed during storage and are generally not present in large quantities before harvest in the field. They cause quality loss through their production of toxic byproducts which has high risk for human and animal health. The most common storage fungi are species of Aspergillus and Penicillium. This short study was conducted with an objective of isolating and identifying the major fungi genera associated with stored maize grain in Seka Chokersa district of Jimma Zone in 2017. Samples were taken from farmers traditional storage containers. For isolation, the fungi were grown on PDA medium and identified morphologically using a dissecting microscope. Accordingly, common stored cereal grain fungi genera Aspergillus, Fusarium and Penicillium were morphologically identified off the samples collected to their genus level. Among these fungi, Aspergillus spp. was identified with high frequency (86.6%) followed by Fusarium spp. (53.3%) and Penicillium spp. (40%). Aspergillus, Fusarium and Penicillium are among the top fungi genera deteriorating stored cereal grain which have high health risk for both human and animal.
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Author Information
  • Holetta Agricultural Research Center, Holetta, Ethiopia

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