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Shigella Serogroups, Entro-Hemoragic E. coli and Their Antibiogram Pattern Among Food Handlers in Food-Handling Establishments in Southern Ethiopia

Received: 8 February 2017     Accepted: 18 February 2017     Published: 11 March 2017
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Abstract

Food-borne illnesses have a dramatic impact both in developing and developed countries. Food handling personnel take part in the transmission of pathogenic food born bacteria in the community. Shigella and E. coli 157:H7 are more significant and well-recognized foodborne pathogens for reasons of their severe consequences of all age groups, high antibiotic resistance and their low infectious dose. Accordingly, food-handlers employing in meal serving facilities could be potential sources of infections of these enteric bacterial pathogens. Community based cross-sectional study was carried out from July 2014 to June 2015 to assess the sero-group and antimicrobial resistance pattern of enteric bacterial pathogens in Wolaita Sodo town among 398 food-handlers working in selected food handling establishments. Pre-tested structured questionnaire was used to collect Socio-demographic characteristics and associated factors. Stool specimens were collected by a clean, dry, wide-mouthed container. Stool culture was done using differential, selective and enrichment medium. Analytical Profile Index 20E biochemical panel was used for identification and differentiation of members of enteric bacterial pathogens. Antibiotic susceptibility testing was done by single disk diffusion technique. Data entry and analysis were done using SPSS version 20. 11 Shigella species and 24 E. coli O157:H7 isolates were detected. S. flexneri, S. sonnei, S. dysenteriae, and S. boydii isolates were isolated. A significant proportion of Ampicillin and Amoxicillin were noticed for all enteric bacterial pathogens. Multidrug resistances prevalence of 72.7%, and 58.3% were observed for Shigella, and E. coli O157: H7 respectively. Raw meat eating habit, hand washing after toilet and hand washing after touching dirty materials have shown significant association with enteric bacterial pathogens prevalence. Shigella and E. coli species were identified from fecal specimen. Significant proportion of multidrug resistances was detected in Shigella and E. coli O157: H7 respectively. Thus screening of food handles is important in order to prevent the transmission of enteric bacterial pathogens and treatment needs to be based on accurate laboratory detection of etiologic agents to mitigate the spread of drug resistant strains.

Published in American Journal of Life Sciences (Volume 5, Issue 2)
DOI 10.11648/j.ajls.20170502.12
Page(s) 46-51
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

E. coli O157:H7, Shigella, Food Handlers, Antibiotics

References
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[14] Aklilu, A., Kahase, D., and Dessalegn, M. (2015): Prevalence of intestinal parasites, salmonella and shigella among apparently health food handlers of Addis Ababa University student’s cafeteria, Addis Ababa, Ethiopia. BMC Research Notes. 8: 17.
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[16] Andualem, T. D., Tiruneh, M. W., Moges, F. Y., Muluye, D. F., Ayalew G. G (2014): Prevalence and antimicrobial susceptibility patterns of Shigella and Salmonella Species among patients with diarrhea attending Gondar town health institutions, Northwest Ethiopia. Science Journal of Public Health. 2 (5): 469-75.
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    Fiseha Wadillo Wada, Tesfahun Molla Bobe, Habtamu Azene Tekle, Tamene Naba Yaya. (2017). Shigella Serogroups, Entro-Hemoragic E. coli and Their Antibiogram Pattern Among Food Handlers in Food-Handling Establishments in Southern Ethiopia. American Journal of Life Sciences, 5(2), 46-51. https://doi.org/10.11648/j.ajls.20170502.12

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    ACS Style

    Fiseha Wadillo Wada; Tesfahun Molla Bobe; Habtamu Azene Tekle; Tamene Naba Yaya. Shigella Serogroups, Entro-Hemoragic E. coli and Their Antibiogram Pattern Among Food Handlers in Food-Handling Establishments in Southern Ethiopia. Am. J. Life Sci. 2017, 5(2), 46-51. doi: 10.11648/j.ajls.20170502.12

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    AMA Style

    Fiseha Wadillo Wada, Tesfahun Molla Bobe, Habtamu Azene Tekle, Tamene Naba Yaya. Shigella Serogroups, Entro-Hemoragic E. coli and Their Antibiogram Pattern Among Food Handlers in Food-Handling Establishments in Southern Ethiopia. Am J Life Sci. 2017;5(2):46-51. doi: 10.11648/j.ajls.20170502.12

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  • @article{10.11648/j.ajls.20170502.12,
      author = {Fiseha Wadillo Wada and Tesfahun Molla Bobe and Habtamu Azene Tekle and Tamene Naba Yaya},
      title = {Shigella Serogroups, Entro-Hemoragic E. coli and Their Antibiogram Pattern Among Food Handlers in Food-Handling Establishments in Southern Ethiopia},
      journal = {American Journal of Life Sciences},
      volume = {5},
      number = {2},
      pages = {46-51},
      doi = {10.11648/j.ajls.20170502.12},
      url = {https://doi.org/10.11648/j.ajls.20170502.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20170502.12},
      abstract = {Food-borne illnesses have a dramatic impact both in developing and developed countries. Food handling personnel take part in the transmission of pathogenic food born bacteria in the community. Shigella and E. coli 157:H7 are more significant and well-recognized foodborne pathogens for reasons of their severe consequences of all age groups, high antibiotic resistance and their low infectious dose. Accordingly, food-handlers employing in meal serving facilities could be potential sources of infections of these enteric bacterial pathogens. Community based cross-sectional study was carried out from July 2014 to June 2015 to assess the sero-group and antimicrobial resistance pattern of enteric bacterial pathogens in Wolaita Sodo town among 398 food-handlers working in selected food handling establishments. Pre-tested structured questionnaire was used to collect Socio-demographic characteristics and associated factors. Stool specimens were collected by a clean, dry, wide-mouthed container. Stool culture was done using differential, selective and enrichment medium. Analytical Profile Index 20E biochemical panel was used for identification and differentiation of members of enteric bacterial pathogens. Antibiotic susceptibility testing was done by single disk diffusion technique. Data entry and analysis were done using SPSS version 20. 11 Shigella species and 24 E. coli O157:H7 isolates were detected. S. flexneri, S. sonnei, S. dysenteriae, and S. boydii isolates were isolated. A significant proportion of Ampicillin and Amoxicillin were noticed for all enteric bacterial pathogens. Multidrug resistances prevalence of 72.7%, and 58.3% were observed for Shigella, and E. coli O157: H7 respectively. Raw meat eating habit, hand washing after toilet and hand washing after touching dirty materials have shown significant association with enteric bacterial pathogens prevalence. Shigella and E. coli species were identified from fecal specimen. Significant proportion of multidrug resistances was detected in Shigella and E. coli O157: H7 respectively. Thus screening of food handles is important in order to prevent the transmission of enteric bacterial pathogens and treatment needs to be based on accurate laboratory detection of etiologic agents to mitigate the spread of drug resistant strains.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Shigella Serogroups, Entro-Hemoragic E. coli and Their Antibiogram Pattern Among Food Handlers in Food-Handling Establishments in Southern Ethiopia
    AU  - Fiseha Wadillo Wada
    AU  - Tesfahun Molla Bobe
    AU  - Habtamu Azene Tekle
    AU  - Tamene Naba Yaya
    Y1  - 2017/03/11
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ajls.20170502.12
    DO  - 10.11648/j.ajls.20170502.12
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 46
    EP  - 51
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20170502.12
    AB  - Food-borne illnesses have a dramatic impact both in developing and developed countries. Food handling personnel take part in the transmission of pathogenic food born bacteria in the community. Shigella and E. coli 157:H7 are more significant and well-recognized foodborne pathogens for reasons of their severe consequences of all age groups, high antibiotic resistance and their low infectious dose. Accordingly, food-handlers employing in meal serving facilities could be potential sources of infections of these enteric bacterial pathogens. Community based cross-sectional study was carried out from July 2014 to June 2015 to assess the sero-group and antimicrobial resistance pattern of enteric bacterial pathogens in Wolaita Sodo town among 398 food-handlers working in selected food handling establishments. Pre-tested structured questionnaire was used to collect Socio-demographic characteristics and associated factors. Stool specimens were collected by a clean, dry, wide-mouthed container. Stool culture was done using differential, selective and enrichment medium. Analytical Profile Index 20E biochemical panel was used for identification and differentiation of members of enteric bacterial pathogens. Antibiotic susceptibility testing was done by single disk diffusion technique. Data entry and analysis were done using SPSS version 20. 11 Shigella species and 24 E. coli O157:H7 isolates were detected. S. flexneri, S. sonnei, S. dysenteriae, and S. boydii isolates were isolated. A significant proportion of Ampicillin and Amoxicillin were noticed for all enteric bacterial pathogens. Multidrug resistances prevalence of 72.7%, and 58.3% were observed for Shigella, and E. coli O157: H7 respectively. Raw meat eating habit, hand washing after toilet and hand washing after touching dirty materials have shown significant association with enteric bacterial pathogens prevalence. Shigella and E. coli species were identified from fecal specimen. Significant proportion of multidrug resistances was detected in Shigella and E. coli O157: H7 respectively. Thus screening of food handles is important in order to prevent the transmission of enteric bacterial pathogens and treatment needs to be based on accurate laboratory detection of etiologic agents to mitigate the spread of drug resistant strains.
    VL  - 5
    IS  - 2
    ER  - 

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Author Information
  • School of Medicine, College of Health Sciences and Medicine, Wolaita Sodo University, Wolaita Sodo, Ethiopia

  • School of Medicine, College of Health Sciences and Medicine, Wolaita Sodo University, Wolaita Sodo, Ethiopia

  • School of Medicine, College of Health Sciences and Medicine, Wolaita Sodo University, Wolaita Sodo, Ethiopia

  • School of Medicine, College of Health Sciences and Medicine, Wolaita Sodo University, Wolaita Sodo, Ethiopia

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