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Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid

Received: 5 November 2014     Accepted: 18 November 2014     Published: 21 November 2014
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Abstract

Acquiring new genetic traits by lateral gene transfer is a bacterial strategy for environmental adaptations. We previously showed that Escherichia coli laterally transmits nonconjugative plasmids in cocultures that contain strains with or without the plasmid. Using a pMB1-derived plasmid and the Keio collection, a comprehensive library of E. coli knockout mutants for nonessential genes, we recently screened for genes responsible for promoting or repressing cell-to-cell plasmid transfer in recipient cells. In this study, we used a pSC101-derived plasmid, instead of a pMB1-derived plasmid, to screen for repressing genes and identified 29 “transfer-up” mutants. Among these, four mutants are common to those previously screened using a pMB1-derived plasmid. Although the roles of the 29 gene products in plasmid transfer mechanism remain uncertain, it is interesting that 28 of the 29 screened genes map to two limited regions on the E. coli chromosome: 18 genes at 34.25–35.31 min and 10 genes at 12.62–13.35 min. Because these two regions commonly contain termination (Ter) sites for DNA replication (TerC: 34.64 min and TerH: 12.91 min), it is possible that chromosomal mutations around specific Ter sites may affect plasmid acquisition in the recipient cells.

Published in American Journal of Life Sciences (Volume 2, Issue 6)
DOI 10.11648/j.ajls.20140206.13
Page(s) 345-350
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Lateral Gene Transfer, Keio Collection, pSC101-Derived Plasmid, Ter Site, Escherichia coli

References
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    Yuka Shibata, Akiko Matsumoto, Mutsumi Horino, Akiko Hirabayashi, Kozue Shirota, et al. (2014). Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid. American Journal of Life Sciences, 2(6), 345-350. https://doi.org/10.11648/j.ajls.20140206.13

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    ACS Style

    Yuka Shibata; Akiko Matsumoto; Mutsumi Horino; Akiko Hirabayashi; Kozue Shirota, et al. Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid. Am. J. Life Sci. 2014, 2(6), 345-350. doi: 10.11648/j.ajls.20140206.13

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    AMA Style

    Yuka Shibata, Akiko Matsumoto, Mutsumi Horino, Akiko Hirabayashi, Kozue Shirota, et al. Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid. Am J Life Sci. 2014;2(6):345-350. doi: 10.11648/j.ajls.20140206.13

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  • @article{10.11648/j.ajls.20140206.13,
      author = {Yuka Shibata and Akiko Matsumoto and Mutsumi Horino and Akiko Hirabayashi and Kozue Shirota and Chinatsu Kawano and Sumio Maeda},
      title = {Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid},
      journal = {American Journal of Life Sciences},
      volume = {2},
      number = {6},
      pages = {345-350},
      doi = {10.11648/j.ajls.20140206.13},
      url = {https://doi.org/10.11648/j.ajls.20140206.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140206.13},
      abstract = {Acquiring new genetic traits by lateral gene transfer is a bacterial strategy for environmental adaptations. We previously showed that Escherichia coli laterally transmits nonconjugative plasmids in cocultures that contain strains with or without the plasmid. Using a pMB1-derived plasmid and the Keio collection, a comprehensive library of E. coli knockout mutants for nonessential genes, we recently screened for genes responsible for promoting or repressing cell-to-cell plasmid transfer in recipient cells. In this study, we used a pSC101-derived plasmid, instead of a pMB1-derived plasmid, to screen for repressing genes and identified 29 “transfer-up” mutants. Among these, four mutants are common to those previously screened using a pMB1-derived plasmid. Although the roles of the 29 gene products in plasmid transfer mechanism remain uncertain, it is interesting that 28 of the 29 screened genes map to two limited regions on the E. coli chromosome: 18 genes at 34.25–35.31 min and 10 genes at 12.62–13.35 min. Because these two regions commonly contain termination (Ter) sites for DNA replication (TerC: 34.64 min and TerH: 12.91 min), it is possible that chromosomal mutations around specific Ter sites may affect plasmid acquisition in the recipient cells.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid
    AU  - Yuka Shibata
    AU  - Akiko Matsumoto
    AU  - Mutsumi Horino
    AU  - Akiko Hirabayashi
    AU  - Kozue Shirota
    AU  - Chinatsu Kawano
    AU  - Sumio Maeda
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    DO  - 10.11648/j.ajls.20140206.13
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 345
    EP  - 350
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20140206.13
    AB  - Acquiring new genetic traits by lateral gene transfer is a bacterial strategy for environmental adaptations. We previously showed that Escherichia coli laterally transmits nonconjugative plasmids in cocultures that contain strains with or without the plasmid. Using a pMB1-derived plasmid and the Keio collection, a comprehensive library of E. coli knockout mutants for nonessential genes, we recently screened for genes responsible for promoting or repressing cell-to-cell plasmid transfer in recipient cells. In this study, we used a pSC101-derived plasmid, instead of a pMB1-derived plasmid, to screen for repressing genes and identified 29 “transfer-up” mutants. Among these, four mutants are common to those previously screened using a pMB1-derived plasmid. Although the roles of the 29 gene products in plasmid transfer mechanism remain uncertain, it is interesting that 28 of the 29 screened genes map to two limited regions on the E. coli chromosome: 18 genes at 34.25–35.31 min and 10 genes at 12.62–13.35 min. Because these two regions commonly contain termination (Ter) sites for DNA replication (TerC: 34.64 min and TerH: 12.91 min), it is possible that chromosomal mutations around specific Ter sites may affect plasmid acquisition in the recipient cells.
    VL  - 2
    IS  - 6
    ER  - 

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Author Information
  • Graduate School of Humanities and Sciences, Nara Women's University, Nara, Japan

  • Graduate School of Humanities and Sciences, Nara Women's University, Nara, Japan

  • Faculty of Human Life and Environment, Nara Women's University, Nara, Japan

  • Faculty of Human Life and Environment, Nara Women's University, Nara, Japan

  • Faculty of Human Life and Environment, Nara Women's University, Nara, Japan

  • Faculty of Human Life and Environment, Nara Women's University, Nara, Japan

  • Graduate School of Humanities and Sciences, Nara Women's University, Nara, Japan

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