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Antisickling, Antioxidant and Antibacterial Activities of Afromomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels

Received: 24 August 2017     Accepted: 20 September 2017     Published: 17 October 2017
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Abstract

In accordance with statistics from the World Health Organization, nearly 80% of populations depend on traditional medicine for primary health care. In order to scientifically validate the phyto-therapeutic wealth of the Democratic Republic of the Congo, three plants were chosen namely: Afromomum alboviolaceum, Annona senegalensis and Mondia whitei. The objective of our study was to assess the antisickling, antioxidant and antibacterial activities of the ethanolic extracts and the dichloromethane fractions of these three plants. Sickle cell blood was supplied from Centre de Médecine Mixte and Anémie SS of Yolo Sud, Kinshasa. Three bacterial strains were used including Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 8739 and Pseudomonas aeroginosa ATCC 9027. The ethanolic extracts of A. alboviolaceum and A. senegalensis and dichloromethane fractions of A. alboviolaceum and M. whitei showed IC50 values < 100 μg/mL in the ABTS test. Only the dichloromethane fraction of A. senengalensis showed an IC50 value of less than 100 μg/mL for the DPPH test. The inhibitory concentration 50 (IC50) values obtained from the ABTS test are lower than those of the DPPH test. All tested extracts possess a high antisickling activity and only soluble dichloromethane extracts are active vis-à-vis Staphylococcus aureus (MIC = 31.5 μg/mL). These results constitute a scientific evidence validating the use of these three medicinal plants for the management of sickle-cell anemia in the Democratic Republic of the Congo.

Published in American Journal of Laboratory Medicine (Volume 2, Issue 4)
DOI 10.11648/j.ajlm.20170204.13
Page(s) 52-59
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Antisickling Activity, Antioxidant Activity, Antibacterial Activity, Inhibitory Concentration, ABTS, DPPH

References
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Cite This Article
  • APA Style

    Gédéon Bongo, Clément Inkoto, Colette Masengo, Claudine Tshiama, Emmanuel Lengbiye, et al. (2017). Antisickling, Antioxidant and Antibacterial Activities of Afromomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels. American Journal of Laboratory Medicine, 2(4), 52-59. https://doi.org/10.11648/j.ajlm.20170204.13

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    ACS Style

    Gédéon Bongo; Clément Inkoto; Colette Masengo; Claudine Tshiama; Emmanuel Lengbiye, et al. Antisickling, Antioxidant and Antibacterial Activities of Afromomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels. Am. J. Lab. Med. 2017, 2(4), 52-59. doi: 10.11648/j.ajlm.20170204.13

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    AMA Style

    Gédéon Bongo, Clément Inkoto, Colette Masengo, Claudine Tshiama, Emmanuel Lengbiye, et al. Antisickling, Antioxidant and Antibacterial Activities of Afromomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels. Am J Lab Med. 2017;2(4):52-59. doi: 10.11648/j.ajlm.20170204.13

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  • @article{10.11648/j.ajlm.20170204.13,
      author = {Gédéon Bongo and Clément Inkoto and Colette Masengo and Claudine Tshiama and Emmanuel Lengbiye and Ruphin Djolu and Mutwale Kapepula and Kabamba Ngombe and Théophile Mbemba and Dorothée Tshilanda and Pius Mpiana and Koto-te-Nyiwa Ngbolua},
      title = {Antisickling, Antioxidant and Antibacterial Activities of Afromomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels},
      journal = {American Journal of Laboratory Medicine},
      volume = {2},
      number = {4},
      pages = {52-59},
      doi = {10.11648/j.ajlm.20170204.13},
      url = {https://doi.org/10.11648/j.ajlm.20170204.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20170204.13},
      abstract = {In accordance with statistics from the World Health Organization, nearly 80% of populations depend on traditional medicine for primary health care. In order to scientifically validate the phyto-therapeutic wealth of the Democratic Republic of the Congo, three plants were chosen namely: Afromomum alboviolaceum, Annona senegalensis and Mondia whitei. The objective of our study was to assess the antisickling, antioxidant and antibacterial activities of the ethanolic extracts and the dichloromethane fractions of these three plants. Sickle cell blood was supplied from Centre de Médecine Mixte and Anémie SS of Yolo Sud, Kinshasa. Three bacterial strains were used including Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 8739 and Pseudomonas aeroginosa ATCC 9027. The ethanolic extracts of A. alboviolaceum and A. senegalensis and dichloromethane fractions of A. alboviolaceum and M. whitei showed IC50 values < 100 μg/mL in the ABTS test. Only the dichloromethane fraction of A. senengalensis showed an IC50 value of less than 100 μg/mL for the DPPH test. The inhibitory concentration 50 (IC50) values obtained from the ABTS test are lower than those of the DPPH test. All tested extracts possess a high antisickling activity and only soluble dichloromethane extracts are active vis-à-vis Staphylococcus aureus (MIC = 31.5 μg/mL). These results constitute a scientific evidence validating the use of these three medicinal plants for the management of sickle-cell anemia in the Democratic Republic of the Congo.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Antisickling, Antioxidant and Antibacterial Activities of Afromomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels
    AU  - Gédéon Bongo
    AU  - Clément Inkoto
    AU  - Colette Masengo
    AU  - Claudine Tshiama
    AU  - Emmanuel Lengbiye
    AU  - Ruphin Djolu
    AU  - Mutwale Kapepula
    AU  - Kabamba Ngombe
    AU  - Théophile Mbemba
    AU  - Dorothée Tshilanda
    AU  - Pius Mpiana
    AU  - Koto-te-Nyiwa Ngbolua
    Y1  - 2017/10/17
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ajlm.20170204.13
    DO  - 10.11648/j.ajlm.20170204.13
    T2  - American Journal of Laboratory Medicine
    JF  - American Journal of Laboratory Medicine
    JO  - American Journal of Laboratory Medicine
    SP  - 52
    EP  - 59
    PB  - Science Publishing Group
    SN  - 2575-386X
    UR  - https://doi.org/10.11648/j.ajlm.20170204.13
    AB  - In accordance with statistics from the World Health Organization, nearly 80% of populations depend on traditional medicine for primary health care. In order to scientifically validate the phyto-therapeutic wealth of the Democratic Republic of the Congo, three plants were chosen namely: Afromomum alboviolaceum, Annona senegalensis and Mondia whitei. The objective of our study was to assess the antisickling, antioxidant and antibacterial activities of the ethanolic extracts and the dichloromethane fractions of these three plants. Sickle cell blood was supplied from Centre de Médecine Mixte and Anémie SS of Yolo Sud, Kinshasa. Three bacterial strains were used including Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 8739 and Pseudomonas aeroginosa ATCC 9027. The ethanolic extracts of A. alboviolaceum and A. senegalensis and dichloromethane fractions of A. alboviolaceum and M. whitei showed IC50 values < 100 μg/mL in the ABTS test. Only the dichloromethane fraction of A. senengalensis showed an IC50 value of less than 100 μg/mL for the DPPH test. The inhibitory concentration 50 (IC50) values obtained from the ABTS test are lower than those of the DPPH test. All tested extracts possess a high antisickling activity and only soluble dichloromethane extracts are active vis-à-vis Staphylococcus aureus (MIC = 31.5 μg/mL). These results constitute a scientific evidence validating the use of these three medicinal plants for the management of sickle-cell anemia in the Democratic Republic of the Congo.
    VL  - 2
    IS  - 4
    ER  - 

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Author Information
  • Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Department of Environmental Sciences, University of Gbadolite, Nord-Ubangi, Democratic Republic of the Congo

  • Enseignement et Administration en Soins Infirmiers, Section Sciences Infirmières, Institut Supérieur des Techniques Médicales, Kinshasa, Democratic Republic of the Congo

  • Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Department of Environmental Sciences, University of Gbadolite, Nord-Ubangi, Democratic Republic of the Congo

  • Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Department of Chemistry, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Department of Chemistry, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

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