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Effect of Gonadal Hormones on Colonic Contractile Activity in Albino Rats

Received: 3 March 2017     Accepted: 27 March 2017     Published: 17 April 2017
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Abstract

Sex differences were reported in Irritable Bowel Syndrome. We aimed to demonstrate the effect of gonadal hormones on colonic contractile activity. In this study, we performed in vitro measurements of colonic contractile activity in longitudinal smooth muscle strips of female and male Albino rats. After administration of a gonadal hormone (estrogen (EST), progesterone (PROG) and testosterone (TEST)) or ethanol (ETH) solution as control, stimulation with acetylcholine (ACh) or inhibition with norepinephrine (NE) was performed. We found that increasing doses of EST resulted in a stepwise inhibition of SCCA in both sexes and inhibited the ACh effect in females. Similarly, high dose PROG inhibited SCCA in females. TEST inhibited SCCA in males but not Ach induced contractions. TEST reduced the inhibitory effect of NE in female rats. In conclusion, exposure of colonic smooth muscle strips to gonadal hormones led to sex-dependent changes in SCCA and modified the response of smooth muscle strips to both pro-contractile and anti-contractile neurotransmitters.

Published in American Journal of Internal Medicine (Volume 5, Issue 2)
DOI 10.11648/j.ajim.20170502.13
Page(s) 26-36
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Colonic Smooth Muscle, Estrogen, Bowel Disorders, Gonadal Hormones, Irritable Bowel Syndrome, Motility

References
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  • APA Style

    Mohamad Yosof Rezk. (2017). Effect of Gonadal Hormones on Colonic Contractile Activity in Albino Rats. American Journal of Internal Medicine, 5(2), 26-36. https://doi.org/10.11648/j.ajim.20170502.13

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    ACS Style

    Mohamad Yosof Rezk. Effect of Gonadal Hormones on Colonic Contractile Activity in Albino Rats. Am. J. Intern. Med. 2017, 5(2), 26-36. doi: 10.11648/j.ajim.20170502.13

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    AMA Style

    Mohamad Yosof Rezk. Effect of Gonadal Hormones on Colonic Contractile Activity in Albino Rats. Am J Intern Med. 2017;5(2):26-36. doi: 10.11648/j.ajim.20170502.13

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  • @article{10.11648/j.ajim.20170502.13,
      author = {Mohamad Yosof Rezk},
      title = {Effect of Gonadal Hormones on Colonic Contractile Activity in Albino Rats},
      journal = {American Journal of Internal Medicine},
      volume = {5},
      number = {2},
      pages = {26-36},
      doi = {10.11648/j.ajim.20170502.13},
      url = {https://doi.org/10.11648/j.ajim.20170502.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajim.20170502.13},
      abstract = {Sex differences were reported in Irritable Bowel Syndrome. We aimed to demonstrate the effect of gonadal hormones on colonic contractile activity. In this study, we performed in vitro measurements of colonic contractile activity in longitudinal smooth muscle strips of female and male Albino rats. After administration of a gonadal hormone (estrogen (EST), progesterone (PROG) and testosterone (TEST)) or ethanol (ETH) solution as control, stimulation with acetylcholine (ACh) or inhibition with norepinephrine (NE) was performed. We found that increasing doses of EST resulted in a stepwise inhibition of SCCA in both sexes and inhibited the ACh effect in females. Similarly, high dose PROG inhibited SCCA in females. TEST inhibited SCCA in males but not Ach induced contractions. TEST reduced the inhibitory effect of NE in female rats. In conclusion, exposure of colonic smooth muscle strips to gonadal hormones led to sex-dependent changes in SCCA and modified the response of smooth muscle strips to both pro-contractile and anti-contractile neurotransmitters.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Effect of Gonadal Hormones on Colonic Contractile Activity in Albino Rats
    AU  - Mohamad Yosof Rezk
    Y1  - 2017/04/17
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ajim.20170502.13
    DO  - 10.11648/j.ajim.20170502.13
    T2  - American Journal of Internal Medicine
    JF  - American Journal of Internal Medicine
    JO  - American Journal of Internal Medicine
    SP  - 26
    EP  - 36
    PB  - Science Publishing Group
    SN  - 2330-4324
    UR  - https://doi.org/10.11648/j.ajim.20170502.13
    AB  - Sex differences were reported in Irritable Bowel Syndrome. We aimed to demonstrate the effect of gonadal hormones on colonic contractile activity. In this study, we performed in vitro measurements of colonic contractile activity in longitudinal smooth muscle strips of female and male Albino rats. After administration of a gonadal hormone (estrogen (EST), progesterone (PROG) and testosterone (TEST)) or ethanol (ETH) solution as control, stimulation with acetylcholine (ACh) or inhibition with norepinephrine (NE) was performed. We found that increasing doses of EST resulted in a stepwise inhibition of SCCA in both sexes and inhibited the ACh effect in females. Similarly, high dose PROG inhibited SCCA in females. TEST inhibited SCCA in males but not Ach induced contractions. TEST reduced the inhibitory effect of NE in female rats. In conclusion, exposure of colonic smooth muscle strips to gonadal hormones led to sex-dependent changes in SCCA and modified the response of smooth muscle strips to both pro-contractile and anti-contractile neurotransmitters.
    VL  - 5
    IS  - 2
    ER  - 

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Author Information
  • Physiology Unit, Basic Medical Sciences Department, College of Medicine and Medical Sciences in Unaizah, Qassim University, Unaizah, KSA

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