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Bioremediation Potentials of Heavy Metal Tolerant Bacteria Isolated from Petroleum Refinery Effluent

Received: 8 February 2016     Accepted: 24 February 2016     Published: 30 March 2016
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Abstract

Five heavy metals tolerant bacteria were isolated from petroleum refinery effluent and identified as Pseudomonas aeruginosa, Staphylococcus aureus, E. coli, Proteus vulgaris and Klebsiella pneumoniae. Each isolate was inoculated into different concentrations of cadmium, chromium, nickel and zinc to determine its maximum tolerance for each heavy metal. All five isolates had low maximum tolerance concentration for cadmium (0.9 mg/L) when compared to chromium (5 mg/L), nickel (5 mg/L) and zinc (7 mg/L). Mixed culture consortium (MCC) remediated high percentage of cadmium (100%), chromium (33.4%), nickel (73.9%), and zinc (90.1%) from the petroleum refinery effluent than pure culture isolates. Among the pure culture isolates Pseudomonas aeruginosa (Cd (100%), Cr (23.1%), Ni (64.3%) & Zn (53.9%) yielded high values for the reduction of heavy metals in the refinery effluent when compared to Staphylococcus aureus, E.coli, Proteus vulgaris and Klebsiella pneumoniae. The isolated bacteria were effective for the remediation of heavy metals from petroleum refinery effluent.

Published in American Journal of Environmental Protection (Volume 5, Issue 2)
DOI 10.11648/j.ajep.20160502.12
Page(s) 29-34
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Bioremediation, Heavy Metal, Bacteria, Refinery, Petroleum

References
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  • APA Style

    Enimie Endurance Oaikhena, Dominic Bawa Makaije, Samuel Dangmwan Denwe, Muhammad Muktar Namadi, Ali Ahmed Haroun. (2016). Bioremediation Potentials of Heavy Metal Tolerant Bacteria Isolated from Petroleum Refinery Effluent. American Journal of Environmental Protection, 5(2), 29-34. https://doi.org/10.11648/j.ajep.20160502.12

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    ACS Style

    Enimie Endurance Oaikhena; Dominic Bawa Makaije; Samuel Dangmwan Denwe; Muhammad Muktar Namadi; Ali Ahmed Haroun. Bioremediation Potentials of Heavy Metal Tolerant Bacteria Isolated from Petroleum Refinery Effluent. Am. J. Environ. Prot. 2016, 5(2), 29-34. doi: 10.11648/j.ajep.20160502.12

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    AMA Style

    Enimie Endurance Oaikhena, Dominic Bawa Makaije, Samuel Dangmwan Denwe, Muhammad Muktar Namadi, Ali Ahmed Haroun. Bioremediation Potentials of Heavy Metal Tolerant Bacteria Isolated from Petroleum Refinery Effluent. Am J Environ Prot. 2016;5(2):29-34. doi: 10.11648/j.ajep.20160502.12

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  • @article{10.11648/j.ajep.20160502.12,
      author = {Enimie Endurance Oaikhena and Dominic Bawa Makaije and Samuel Dangmwan Denwe and Muhammad Muktar Namadi and Ali Ahmed Haroun},
      title = {Bioremediation Potentials of Heavy Metal Tolerant Bacteria Isolated from Petroleum Refinery Effluent},
      journal = {American Journal of Environmental Protection},
      volume = {5},
      number = {2},
      pages = {29-34},
      doi = {10.11648/j.ajep.20160502.12},
      url = {https://doi.org/10.11648/j.ajep.20160502.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajep.20160502.12},
      abstract = {Five heavy metals tolerant bacteria were isolated from petroleum refinery effluent and identified as Pseudomonas aeruginosa, Staphylococcus aureus, E. coli, Proteus vulgaris and Klebsiella pneumoniae. Each isolate was inoculated into different concentrations of cadmium, chromium, nickel and zinc to determine its maximum tolerance for each heavy metal. All five isolates had low maximum tolerance concentration for cadmium (0.9 mg/L) when compared to chromium (5 mg/L), nickel (5 mg/L) and zinc (7 mg/L). Mixed culture consortium (MCC) remediated high percentage of cadmium (100%), chromium (33.4%), nickel (73.9%), and zinc (90.1%) from the petroleum refinery effluent than pure culture isolates. Among the pure culture isolates Pseudomonas aeruginosa (Cd (100%), Cr (23.1%), Ni (64.3%) & Zn (53.9%) yielded high values for the reduction of heavy metals in the refinery effluent when compared to Staphylococcus aureus, E.coli, Proteus vulgaris and Klebsiella pneumoniae. The isolated bacteria were effective for the remediation of heavy metals from petroleum refinery effluent.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Bioremediation Potentials of Heavy Metal Tolerant Bacteria Isolated from Petroleum Refinery Effluent
    AU  - Enimie Endurance Oaikhena
    AU  - Dominic Bawa Makaije
    AU  - Samuel Dangmwan Denwe
    AU  - Muhammad Muktar Namadi
    AU  - Ali Ahmed Haroun
    Y1  - 2016/03/30
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ajep.20160502.12
    DO  - 10.11648/j.ajep.20160502.12
    T2  - American Journal of Environmental Protection
    JF  - American Journal of Environmental Protection
    JO  - American Journal of Environmental Protection
    SP  - 29
    EP  - 34
    PB  - Science Publishing Group
    SN  - 2328-5699
    UR  - https://doi.org/10.11648/j.ajep.20160502.12
    AB  - Five heavy metals tolerant bacteria were isolated from petroleum refinery effluent and identified as Pseudomonas aeruginosa, Staphylococcus aureus, E. coli, Proteus vulgaris and Klebsiella pneumoniae. Each isolate was inoculated into different concentrations of cadmium, chromium, nickel and zinc to determine its maximum tolerance for each heavy metal. All five isolates had low maximum tolerance concentration for cadmium (0.9 mg/L) when compared to chromium (5 mg/L), nickel (5 mg/L) and zinc (7 mg/L). Mixed culture consortium (MCC) remediated high percentage of cadmium (100%), chromium (33.4%), nickel (73.9%), and zinc (90.1%) from the petroleum refinery effluent than pure culture isolates. Among the pure culture isolates Pseudomonas aeruginosa (Cd (100%), Cr (23.1%), Ni (64.3%) & Zn (53.9%) yielded high values for the reduction of heavy metals in the refinery effluent when compared to Staphylococcus aureus, E.coli, Proteus vulgaris and Klebsiella pneumoniae. The isolated bacteria were effective for the remediation of heavy metals from petroleum refinery effluent.
    VL  - 5
    IS  - 2
    ER  - 

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Author Information
  • Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria

  • Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria

  • Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria

  • Department of Chemistry, Nigerian Defence Academy, Kaduna, Nigeria

  • Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria

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