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Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract

Received: 19 September 2020     Accepted: 28 September 2020     Published: 12 October 2020
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Abstract

Dalbergia latifolia commonly known as the Indian rosewood, commonly found in association with Teak trees population in and around Western Ghat areas of Tamil Nadu and Kerala in India. From the heartwood of roots and trunk many phytochemical contents were investigated. A wide spectrum of flavanoids namely, flavones, isoflavones, neoflavones and chalcones have been obtained. Specifically, one of the flavonoids compound chalcone compound-Isoliquiritigenin (ILTG, 2',4',4'-trihydroxychalcone) shows various pharmacological properties, including cytotoxic activities. This study revealed the cytotoxic effect of Isoliquiritigenin, efficiently controls the invasive capacity of breast cancer cells MCF10A human mammary epithelial cell line through uplifting G1-phase cell cycle, even arrest at low concentration, also accelerate the extracellular signal regulated kinase signaling pathway to topup the proteins combined with apoptosis and arrest cancer. The cytotoxicity invitro of isoliquitrigenin against MCF10A ATCC cells was investigated by MTT assay. Apoptosis was evaluated by microscopy and flow cytometry. Isoliquiritigenin exhibited the most potent anticancer activity against MCF 10A ATCC with an IC 50 of 10.00 µg/ml. The methanolic extract expressed cytotoxic effect with an IC50 value of 20 µg/ml. The compounds were also tested for their toxicity on normal human cell lines-LO2 and were found to be nontoxic. Administering the wood extract approaches may help reduce side effects in patients under conventional chemotherapy. These potential molecule may exerts their co-active effects through various pathways and helpful for the development of novel drugs; also conservation of these important germplasm is mandatory.

Published in American Journal of Agriculture and Forestry (Volume 8, Issue 5)
DOI 10.11648/j.ajaf.20200805.14
Page(s) 208-213
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Dalbergia latifolia, Isoliquiritigenin, Breast Cancer Cell Line, Cytotoxicity, Conservation

References
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Cite This Article
  • APA Style

    Thangamani Dhandapani, KR Sasidharan, Girija Shanmugam, Vimaladevi Sadanandam, Ragunathan Vasudevan, et al. (2020). Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract. American Journal of Agriculture and Forestry, 8(5), 208-213. https://doi.org/10.11648/j.ajaf.20200805.14

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    ACS Style

    Thangamani Dhandapani; KR Sasidharan; Girija Shanmugam; Vimaladevi Sadanandam; Ragunathan Vasudevan, et al. Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract. Am. J. Agric. For. 2020, 8(5), 208-213. doi: 10.11648/j.ajaf.20200805.14

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    AMA Style

    Thangamani Dhandapani, KR Sasidharan, Girija Shanmugam, Vimaladevi Sadanandam, Ragunathan Vasudevan, et al. Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract. Am J Agric For. 2020;8(5):208-213. doi: 10.11648/j.ajaf.20200805.14

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  • @article{10.11648/j.ajaf.20200805.14,
      author = {Thangamani Dhandapani and KR Sasidharan and Girija Shanmugam and Vimaladevi Sadanandam and Ragunathan Vasudevan and Suryanarayanan Lalitha and Ravichandran Anandalakshmi},
      title = {Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract},
      journal = {American Journal of Agriculture and Forestry},
      volume = {8},
      number = {5},
      pages = {208-213},
      doi = {10.11648/j.ajaf.20200805.14},
      url = {https://doi.org/10.11648/j.ajaf.20200805.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20200805.14},
      abstract = {Dalbergia latifolia commonly known as the Indian rosewood, commonly found in association with Teak trees population in and around Western Ghat areas of Tamil Nadu and Kerala in India. From the heartwood of roots and trunk many phytochemical contents were investigated. A wide spectrum of flavanoids namely, flavones, isoflavones, neoflavones and chalcones have been obtained. Specifically, one of the flavonoids compound chalcone compound-Isoliquiritigenin (ILTG, 2',4',4'-trihydroxychalcone) shows various pharmacological properties, including cytotoxic activities. This study revealed the cytotoxic effect of Isoliquiritigenin, efficiently controls the invasive capacity of breast cancer cells MCF10A human mammary epithelial cell line through uplifting G1-phase cell cycle, even arrest at low concentration, also accelerate the extracellular signal regulated kinase signaling pathway to topup the proteins combined with apoptosis and arrest cancer. The cytotoxicity invitro of isoliquitrigenin against MCF10A ATCC cells was investigated by MTT assay. Apoptosis was evaluated by microscopy and flow cytometry. Isoliquiritigenin exhibited the most potent anticancer activity against MCF 10A ATCC with an IC 50 of 10.00 µg/ml. The methanolic extract expressed cytotoxic effect with an IC50 value of 20 µg/ml. The compounds were also tested for their toxicity on normal human cell lines-LO2 and were found to be nontoxic. Administering the wood extract approaches may help reduce side effects in patients under conventional chemotherapy. These potential molecule may exerts their co-active effects through various pathways and helpful for the development of novel drugs; also conservation of these important germplasm is mandatory.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract
    AU  - Thangamani Dhandapani
    AU  - KR Sasidharan
    AU  - Girija Shanmugam
    AU  - Vimaladevi Sadanandam
    AU  - Ragunathan Vasudevan
    AU  - Suryanarayanan Lalitha
    AU  - Ravichandran Anandalakshmi
    Y1  - 2020/10/12
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ajaf.20200805.14
    DO  - 10.11648/j.ajaf.20200805.14
    T2  - American Journal of Agriculture and Forestry
    JF  - American Journal of Agriculture and Forestry
    JO  - American Journal of Agriculture and Forestry
    SP  - 208
    EP  - 213
    PB  - Science Publishing Group
    SN  - 2330-8591
    UR  - https://doi.org/10.11648/j.ajaf.20200805.14
    AB  - Dalbergia latifolia commonly known as the Indian rosewood, commonly found in association with Teak trees population in and around Western Ghat areas of Tamil Nadu and Kerala in India. From the heartwood of roots and trunk many phytochemical contents were investigated. A wide spectrum of flavanoids namely, flavones, isoflavones, neoflavones and chalcones have been obtained. Specifically, one of the flavonoids compound chalcone compound-Isoliquiritigenin (ILTG, 2',4',4'-trihydroxychalcone) shows various pharmacological properties, including cytotoxic activities. This study revealed the cytotoxic effect of Isoliquiritigenin, efficiently controls the invasive capacity of breast cancer cells MCF10A human mammary epithelial cell line through uplifting G1-phase cell cycle, even arrest at low concentration, also accelerate the extracellular signal regulated kinase signaling pathway to topup the proteins combined with apoptosis and arrest cancer. The cytotoxicity invitro of isoliquitrigenin against MCF10A ATCC cells was investigated by MTT assay. Apoptosis was evaluated by microscopy and flow cytometry. Isoliquiritigenin exhibited the most potent anticancer activity against MCF 10A ATCC with an IC 50 of 10.00 µg/ml. The methanolic extract expressed cytotoxic effect with an IC50 value of 20 µg/ml. The compounds were also tested for their toxicity on normal human cell lines-LO2 and were found to be nontoxic. Administering the wood extract approaches may help reduce side effects in patients under conventional chemotherapy. These potential molecule may exerts their co-active effects through various pathways and helpful for the development of novel drugs; also conservation of these important germplasm is mandatory.
    VL  - 8
    IS  - 5
    ER  - 

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Author Information
  • Forest Genetic Resource Management Division, Institute of Forest Genetics and Tree Breeding, Coimbatore, India

  • Forest Ecology and Climate Change Division, Institute of Forest Genetics and Tree Breeding, Coimbatore, India

  • Department of Biotechnology, Bharathiar University, Coimbatore, India

  • Germplasm Conservation Division, ICAR-NBPGR, New Delhi, India

  • Forest Genetic Resource Management Division, Institute of Forest Genetics and Tree Breeding, Coimbatore, India

  • Forest Genetic Resource Management Division, Institute of Forest Genetics and Tree Breeding, Coimbatore, India

  • Forest Genetic Resource Management Division, Institute of Forest Genetics and Tree Breeding, Coimbatore, India

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