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Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants

Received: 7 May 2018     Accepted: 7 August 2018     Published: 15 September 2018
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Abstract

This study evaluated that grafting as an effective measure on resistance disease in continuous cropping system has a remarkable impact on improving the microbial community population and structure conditions in the rhizosphere of eggplant, which lead to a highly suppressive on the disease incidence. Verticillium dahliae, microbial community and soil enzyme activities in the rhizosphere of both grafted and self-rooted eggplants investigations were conducted in different eggplant growth stages in pot trials to determine the effects of grafting on the soil microbiological properties. In addition, an assessment of the bio-control effects of antagonistic microbes isolated from rhizosphere of grafted eggplants on the incidence of Verticillium wilt was performed. Grafted eggplants exhibited strong resistance (nearly 90%) with a lower pathological incident index. Amounts of Verticillium dahliae in the rhizosphere of grafted eggplants were lower before the fruiting stage compared to non-grafted self-rooting eggplants. Although each general classification of microbes (bacteria, actinomyces and fungi) was reduced over all growth stages by grafting, the ranges of bacterial and actinomycete reduction in the rhizosphere of grafted eggplants were lower compared to self-rooted eggplants. Experimental evidence indicated that grafting increased the relative ratios of Bacteria to Fungi and Actinomycete to Fungi. In terms of functional microbes, amounts of azotobacteria, ammonifying and nitrifying bacteria were significantly higher in grafted eggplants than self-rooted eggplants, especially after floral initiation. Compared to Control, grafting leaded to a significantly positive impacts on invertase, protein enzyme, urease, peroxidase and catalase activities in the rhizosphere of eggplants. Using antagonistic strains have showed strong suppression of Verticillium dahliae growth to study the prevention and curative effects on Verticillium wilt in pot trials.

Published in American Journal of Agriculture and Forestry (Volume 6, Issue 5)
DOI 10.11648/j.ajaf.20180605.13
Page(s) 132-140
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Grafting Eggplants, Verticillium Wilt, Verticillium dahliae, Continuous Cropping, Soil Microorganism

References
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    Yuling Yin, Shaochun Luo, Yunpeng Li, Jinsong Zhou, Yongping Tang, et al. (2018). Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants. American Journal of Agriculture and Forestry, 6(5), 132-140. https://doi.org/10.11648/j.ajaf.20180605.13

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    ACS Style

    Yuling Yin; Shaochun Luo; Yunpeng Li; Jinsong Zhou; Yongping Tang, et al. Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants. Am. J. Agric. For. 2018, 6(5), 132-140. doi: 10.11648/j.ajaf.20180605.13

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    AMA Style

    Yuling Yin, Shaochun Luo, Yunpeng Li, Jinsong Zhou, Yongping Tang, et al. Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants. Am J Agric For. 2018;6(5):132-140. doi: 10.11648/j.ajaf.20180605.13

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  • @article{10.11648/j.ajaf.20180605.13,
      author = {Yuling Yin and Shaochun Luo and Yunpeng Li and Jinsong Zhou and Yongping Tang and Yuan Liu},
      title = {Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants},
      journal = {American Journal of Agriculture and Forestry},
      volume = {6},
      number = {5},
      pages = {132-140},
      doi = {10.11648/j.ajaf.20180605.13},
      url = {https://doi.org/10.11648/j.ajaf.20180605.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20180605.13},
      abstract = {This study evaluated that grafting as an effective measure on resistance disease in continuous cropping system has a remarkable impact on improving the microbial community population and structure conditions in the rhizosphere of eggplant, which lead to a highly suppressive on the disease incidence. Verticillium dahliae, microbial community and soil enzyme activities in the rhizosphere of both grafted and self-rooted eggplants investigations were conducted in different eggplant growth stages in pot trials to determine the effects of grafting on the soil microbiological properties. In addition, an assessment of the bio-control effects of antagonistic microbes isolated from rhizosphere of grafted eggplants on the incidence of Verticillium wilt was performed. Grafted eggplants exhibited strong resistance (nearly 90%) with a lower pathological incident index. Amounts of Verticillium dahliae in the rhizosphere of grafted eggplants were lower before the fruiting stage compared to non-grafted self-rooting eggplants. Although each general classification of microbes (bacteria, actinomyces and fungi) was reduced over all growth stages by grafting, the ranges of bacterial and actinomycete reduction in the rhizosphere of grafted eggplants were lower compared to self-rooted eggplants. Experimental evidence indicated that grafting increased the relative ratios of Bacteria to Fungi and Actinomycete to Fungi. In terms of functional microbes, amounts of azotobacteria, ammonifying and nitrifying bacteria were significantly higher in grafted eggplants than self-rooted eggplants, especially after floral initiation. Compared to Control, grafting leaded to a significantly positive impacts on invertase, protein enzyme, urease, peroxidase and catalase activities in the rhizosphere of eggplants. Using antagonistic strains have showed strong suppression of Verticillium dahliae growth to study the prevention and curative effects on Verticillium wilt in pot trials.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants
    AU  - Yuling Yin
    AU  - Shaochun Luo
    AU  - Yunpeng Li
    AU  - Jinsong Zhou
    AU  - Yongping Tang
    AU  - Yuan Liu
    Y1  - 2018/09/15
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ajaf.20180605.13
    DO  - 10.11648/j.ajaf.20180605.13
    T2  - American Journal of Agriculture and Forestry
    JF  - American Journal of Agriculture and Forestry
    JO  - American Journal of Agriculture and Forestry
    SP  - 132
    EP  - 140
    PB  - Science Publishing Group
    SN  - 2330-8591
    UR  - https://doi.org/10.11648/j.ajaf.20180605.13
    AB  - This study evaluated that grafting as an effective measure on resistance disease in continuous cropping system has a remarkable impact on improving the microbial community population and structure conditions in the rhizosphere of eggplant, which lead to a highly suppressive on the disease incidence. Verticillium dahliae, microbial community and soil enzyme activities in the rhizosphere of both grafted and self-rooted eggplants investigations were conducted in different eggplant growth stages in pot trials to determine the effects of grafting on the soil microbiological properties. In addition, an assessment of the bio-control effects of antagonistic microbes isolated from rhizosphere of grafted eggplants on the incidence of Verticillium wilt was performed. Grafted eggplants exhibited strong resistance (nearly 90%) with a lower pathological incident index. Amounts of Verticillium dahliae in the rhizosphere of grafted eggplants were lower before the fruiting stage compared to non-grafted self-rooting eggplants. Although each general classification of microbes (bacteria, actinomyces and fungi) was reduced over all growth stages by grafting, the ranges of bacterial and actinomycete reduction in the rhizosphere of grafted eggplants were lower compared to self-rooted eggplants. Experimental evidence indicated that grafting increased the relative ratios of Bacteria to Fungi and Actinomycete to Fungi. In terms of functional microbes, amounts of azotobacteria, ammonifying and nitrifying bacteria were significantly higher in grafted eggplants than self-rooted eggplants, especially after floral initiation. Compared to Control, grafting leaded to a significantly positive impacts on invertase, protein enzyme, urease, peroxidase and catalase activities in the rhizosphere of eggplants. Using antagonistic strains have showed strong suppression of Verticillium dahliae growth to study the prevention and curative effects on Verticillium wilt in pot trials.
    VL  - 6
    IS  - 5
    ER  - 

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Author Information
  • Institute of Vegetables and Flowers, Jiangxi Academy of Agricultural Sciences, Nanchang, China

  • Institute of Vegetables and Flowers, Jiangxi Academy of Agricultural Sciences, Nanchang, China

  • College of Agricultural Resources and Environment, Heilongjiang University, Harbin, China

  • Institute of Vegetables and Flowers, Jiangxi Academy of Agricultural Sciences, Nanchang, China

  • Institute of Vegetables and Flowers, Jiangxi Academy of Agricultural Sciences, Nanchang, China

  • Liaoning Saline or Alkaline Land Utilization and Research Institute, Liaoning Academy of Agricultural Sciences, Panjin, China

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