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Phytochemical Composition, Biological Activities of Croton lobatus L. Leaves, Hydrolysis Effect on Activities and Chemical Composition

Received: 5 February 2020     Accepted: 27 February 2020     Published: 10 March 2020
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Abstract

Croton lobatus L. is medicinal plant widely used in traditional medicine, but little known in literature for its biological properties. Aims were to study its phytochemical composition and some biological properties and influence of acid hydrolysis on biological activities and chemical composition of Croton lobatus L. extracts. Successive extractions with solvents of increasing polarity (cyclohexane, ethyl acetate, and methanol) were carried out on powder of Croton lobatus L. leaves. Evaluation of xanthine oxidase, acetylcholinesterase, 5-lipoxygenase and α-amylase inhibitions, antioxidant activity was performed. Acid hydrolysis effect was evaluated by comparison of chemical composition and xanthine oxidase, α-amylase inhibition activities of hydrolyzed and unhydrolyzed extracts. Results showed that at 50 mg/L Croton lobatus L. has very small antioxidant activity (3.4 - 4.9%), small inhibitory activity of 5-lipoxygenase (6.3-6.9%), inhibitory activity of acetylcholinesterase between (2.7 - 31.3%), moderate inhibitory activity of α-amylase (52.8 - 64.3%), moderate inhibitory activity of xanthine oxidase (22.2 - 62.6%). Croton lobatus L. could be used in diabetes and gout treatments, given results of α-amylase inhibition and xanthine oxidase. Acid hydrolysis has negative effect on process of xanthine oxidase inhibition and on chemical composition.

Published in American Journal of Applied Chemistry (Volume 8, Issue 1)
DOI 10.11648/j.ajac.20200801.13
Page(s) 13-22
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Croton lobatus L., Antioxidant, Acetylcholinesterase, Xanthine Oxidase, α-amylase, 5-Lipoxygenase, Inhibition, Acid Hydrolysis

References
[1] Akoègninou A., Van der Burg W. J., Van der Maesen L. J. G. Backhuys Flore analytique du Bénin, 2006, 1034 pages.
[2] Adjanohoun E. J, Adjakidje V., Ahyi, M. R. A., Aké assi, L., Akoegninou, A., D’Almeida, J., Apovo, F., Boukef, K., Chadare, M., Cusset, G., Dramane, K., Eyme, J., Gassita, J.-N., Gbaguidi, N., Goudote, E., Guinko, P., Houngnon, P., Lo, I., Keita, A., Kiniffo, H., Kone-Bamba, D., Musampa Nseyya, A., Saadou, M., Sodogandji, T., De Souza, S., Tchabi, A., Zinsou Dossa, C. & Zohoun, T. Contribution aux études ethnobotaniques et floristiques en République Populaire du Bénin. Médecine traditionnelle et pharmacopée, Agence de Coopération Culturelle et Technique, 1989, Paris.
[3] Attioua B. K., Harisolo R., Boti J. B., Adiko V. A., Tonzibo F. Z., Djakoure L. A. Isolation and identification of alkaloids from Croton lobatus, Int. J. Pharm. Sci. Rev. Res., 2012, 13 (2), 1-4.
[4] Weniger B., Lagnika L., Ndjakou B. L., Vonthron C., L’ethnopharmacologie et la recherche de molécules antipaludéennes dans la biodiversité ivoirienne, béninoise et camerounaise. Ethnopharmacologia 2008, 41, 62-70.
[5] Lagnika L., Weniger B., Vonthron-Senecheaub C., Sanni A.; Antiprotozoal activities of compounds isolated from Croton lobatus L. Afr. J. Infect. Dis. 2009, 3 (1): 1 – 5.
[6] Lagnika L., Anago E. and Sanni A., Screening for antibacterial, antioxidant activity and toxicity of some medicinal plants used in Benin folkloric medicine, Journal of Medicinal Plants Research 2011, 5 (5), 773-777.
[7] Chodaton-Zinsou M. D. Assogba F. M., Gbaguidi F., Moudachirou M., Chemical composition and in vitro investigation of biological activities of Hemizygia bracteosa (Benth) Briq leaves, J. Pharmacognosy Phytother, 2018, 11-20.
[8] Wach A., Pyrzynska K., Biesaga M., Quercetin content in some food and herbal samples, Food chemistry, 2007 (100) 699-704.
[9] Deore S. L., S. S. Khadabadi B. A., Baviskar S. S., Khadabadi R. A., Khangenbam U. S., Koli N. P., Daga P. A., Gadbail P. A. Jain In vitro Antioxidant activity and Phenolic Content of Croton caudatum, Inter. J. Chem Tech Research, 2009, 1 (2), 174-176.
[10] Satya K., Srinivas K., Srilakshmi B. J., Lakshmi C. M., Krishna S. A, Phytochemical and pharmacoligical evaluation of Euphorbiaceae family plant leaves Acalypha indica, Croton bonplandianum Baill, Mintage J. Pharm. Med. Sci., 2015, 4 (3), 17-22.
[11] Muhammad Naeem Qaisar, Bashir Ahmad Chaudary, Muhammad Uzair, Sajid Nawaz Hussain Evaluation of Antioxidant and Cytotoxic Capacity of Croton bonplandianum. Baill, Am. J. Plant Sci., 2013, 4, 1709-1712.
[12] José-Mirabeau O. Ramos, Cliomar A. Santos, Danielle G. Santana, Darlisson A. Santos, Péricles B. Alves, Sara M. Thomazzi, Chemical constituents and potential anti inflammatory activity of the essential oil from the leaves of Croton argyrophyllus, Rev. Bras. Farmacogn. Braz. J. Pharmacogn. 2013, 23 (4), 644-650.
[13] Wilmer F. S. P., Luis F. O. G., Claudia C. P. J., Walter M. A., John J. M. A., Phytochemical screening, free radical scavenging, and anti-inflammatory activity of Croton leptostachyus Kunth leaf extracts, Asian J. Pharm. Clin. Res, 2015, 8, 197-201.
[14] Elufioye T. O. Obuoror E. M., Sennuga A. T., Agbedahunsi J. M., Adesanya S. A.,, Acetylcholinesterase and butyrylcholinesterase inhibitory activity of som selected Nigerian medicinal plants, Brazilian journal of pharmacognosy 2010, 20 (4), 472-477.
[15] Bakthir H. Awadh Ali N. A., Arnold N., Teichert A. Wessjohann L., Anticholinesterase activity of endemic plant extracts from Soqotra, Afr. J. Tradit. Complement. Altern. Med. 2011, 8 (3): 296-299.
[16] Myung-Hee K., Sung-Hoon J., Hae-Dong J., Mee Sook L., Young-In K., Antioxidant activity and α-glucosidase inhibitory potential of onion (Allium cepa L.) extracts. Food Sci Biotechnol., 2010, 19, 159–64.
[17] Owen, P. L.; Johns, T. Xanthine oxidase inhibitory activity of northeastern North American plant remedies used for gout. J. Ethnopharmacol. 1999, 64, 149-160.
[18] Umamaheswari, M., AsokKumar, K., Somasundaram, A., Sivashanmugam, T., Subhadradevi, V.; Ravi, T. K. Xanthine oxidase inhibitory activity of some Indian medical plants. J. Ethnopharmacol., 2007, 109, 547–551.
[19] Hoorn D. E. C. V. Nijiveldt R. J., Leeuwen P. A. M. V. Hofman Z., M’Rabet L., Bont D. B. A. D., Norren K. V., Accurate prediction of xanthine oxidase inhibition based on the structure of flavonoids, Eur. J. Pharmacol 2002, 451: 111-118.
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  • APA Style

    Marthe Dominique Chodaton-Zinsou, Fidèle Mahoudo Assogba, Eléonore Yayi-Ladékan, Fernand Gbaguidi, Mansourou Moudachirou, et al. (2020). Phytochemical Composition, Biological Activities of Croton lobatus L. Leaves, Hydrolysis Effect on Activities and Chemical Composition. American Journal of Applied Chemistry, 8(1), 13-22. https://doi.org/10.11648/j.ajac.20200801.13

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    ACS Style

    Marthe Dominique Chodaton-Zinsou; Fidèle Mahoudo Assogba; Eléonore Yayi-Ladékan; Fernand Gbaguidi; Mansourou Moudachirou, et al. Phytochemical Composition, Biological Activities of Croton lobatus L. Leaves, Hydrolysis Effect on Activities and Chemical Composition. Am. J. Appl. Chem. 2020, 8(1), 13-22. doi: 10.11648/j.ajac.20200801.13

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    AMA Style

    Marthe Dominique Chodaton-Zinsou, Fidèle Mahoudo Assogba, Eléonore Yayi-Ladékan, Fernand Gbaguidi, Mansourou Moudachirou, et al. Phytochemical Composition, Biological Activities of Croton lobatus L. Leaves, Hydrolysis Effect on Activities and Chemical Composition. Am J Appl Chem. 2020;8(1):13-22. doi: 10.11648/j.ajac.20200801.13

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  • @article{10.11648/j.ajac.20200801.13,
      author = {Marthe Dominique Chodaton-Zinsou and Fidèle Mahoudo Assogba and Eléonore Yayi-Ladékan and Fernand Gbaguidi and Mansourou Moudachirou and Joachim Djimon Gbénou},
      title = {Phytochemical Composition, Biological Activities of Croton lobatus L. Leaves, Hydrolysis Effect on Activities and Chemical Composition},
      journal = {American Journal of Applied Chemistry},
      volume = {8},
      number = {1},
      pages = {13-22},
      doi = {10.11648/j.ajac.20200801.13},
      url = {https://doi.org/10.11648/j.ajac.20200801.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajac.20200801.13},
      abstract = {Croton lobatus L. is medicinal plant widely used in traditional medicine, but little known in literature for its biological properties. Aims were to study its phytochemical composition and some biological properties and influence of acid hydrolysis on biological activities and chemical composition of Croton lobatus L. extracts. Successive extractions with solvents of increasing polarity (cyclohexane, ethyl acetate, and methanol) were carried out on powder of Croton lobatus L. leaves. Evaluation of xanthine oxidase, acetylcholinesterase, 5-lipoxygenase and α-amylase inhibitions, antioxidant activity was performed. Acid hydrolysis effect was evaluated by comparison of chemical composition and xanthine oxidase, α-amylase inhibition activities of hydrolyzed and unhydrolyzed extracts. Results showed that at 50 mg/L Croton lobatus L. has very small antioxidant activity (3.4 - 4.9%), small inhibitory activity of 5-lipoxygenase (6.3-6.9%), inhibitory activity of acetylcholinesterase between (2.7 - 31.3%), moderate inhibitory activity of α-amylase (52.8 - 64.3%), moderate inhibitory activity of xanthine oxidase (22.2 - 62.6%). Croton lobatus L. could be used in diabetes and gout treatments, given results of α-amylase inhibition and xanthine oxidase. Acid hydrolysis has negative effect on process of xanthine oxidase inhibition and on chemical composition.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Phytochemical Composition, Biological Activities of Croton lobatus L. Leaves, Hydrolysis Effect on Activities and Chemical Composition
    AU  - Marthe Dominique Chodaton-Zinsou
    AU  - Fidèle Mahoudo Assogba
    AU  - Eléonore Yayi-Ladékan
    AU  - Fernand Gbaguidi
    AU  - Mansourou Moudachirou
    AU  - Joachim Djimon Gbénou
    Y1  - 2020/03/10
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ajac.20200801.13
    DO  - 10.11648/j.ajac.20200801.13
    T2  - American Journal of Applied Chemistry
    JF  - American Journal of Applied Chemistry
    JO  - American Journal of Applied Chemistry
    SP  - 13
    EP  - 22
    PB  - Science Publishing Group
    SN  - 2330-8745
    UR  - https://doi.org/10.11648/j.ajac.20200801.13
    AB  - Croton lobatus L. is medicinal plant widely used in traditional medicine, but little known in literature for its biological properties. Aims were to study its phytochemical composition and some biological properties and influence of acid hydrolysis on biological activities and chemical composition of Croton lobatus L. extracts. Successive extractions with solvents of increasing polarity (cyclohexane, ethyl acetate, and methanol) were carried out on powder of Croton lobatus L. leaves. Evaluation of xanthine oxidase, acetylcholinesterase, 5-lipoxygenase and α-amylase inhibitions, antioxidant activity was performed. Acid hydrolysis effect was evaluated by comparison of chemical composition and xanthine oxidase, α-amylase inhibition activities of hydrolyzed and unhydrolyzed extracts. Results showed that at 50 mg/L Croton lobatus L. has very small antioxidant activity (3.4 - 4.9%), small inhibitory activity of 5-lipoxygenase (6.3-6.9%), inhibitory activity of acetylcholinesterase between (2.7 - 31.3%), moderate inhibitory activity of α-amylase (52.8 - 64.3%), moderate inhibitory activity of xanthine oxidase (22.2 - 62.6%). Croton lobatus L. could be used in diabetes and gout treatments, given results of α-amylase inhibition and xanthine oxidase. Acid hydrolysis has negative effect on process of xanthine oxidase inhibition and on chemical composition.
    VL  - 8
    IS  - 1
    ER  - 

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Author Information
  • Faculty of Sciences and Technics, University of Abomey-Calavi, Abomey-Calavi, Benin

  • Faculty of Sciences and Technics, University of Abomey-Calavi, Abomey-Calavi, Benin

  • Faculty of Sciences and Technics, University of Abomey-Calavi, Abomey-Calavi, Benin

  • Faculty of Sciences and Technics, University of Abomey-Calavi, Abomey-Calavi, Benin

  • Faculty of Sciences and Technics, University of Abomey-Calavi, Abomey-Calavi, Benin

  • Faculty of Sciences and Technics, University of Abomey-Calavi, Abomey-Calavi, Benin

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