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The Determination of Caffeine Level of Wolaita Zone, Ethiopia Coffee Using UV-visible Spectrophotometer

Received: 15 February 2016     Accepted: 24 February 2016     Published: 23 March 2016
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Abstract

Coffee is an important commodity culturally, commercially and economically in the world. Because of its consumption in most countries in the world, it is important to investigate the exact amounts of its chemical constituents. Caffeine is one of the main components of coffee that affect the quality of coffee. In this research, rapid, simple, accurate, reproducible and cheap UV-Vis spectrometer was used for the quantification of caffeine in coffee and dichloromethane was used as a solvent for the coffee samples taken from Wolaita zone of five woredas. The results of this research show that the amount of caffeine of the coffee samples ranged from (450.13 to 579.60 (mg/L). The amounts of caffeine in Bolso Bomba coffee is the greatest, of all the other coffee samples studied in Wolaita Zone. On the other hand, Kindo Koisha and that of Bolso sore coffee has the least caffeine content compared to the other coffee samples measured. The caffeine content of Sodo Zuria coffee and Humbo coffee samples has an average amounts of caffeine.

Published in American Journal of Applied Chemistry (Volume 4, Issue 2)
DOI 10.11648/j.ajac.20160402.14
Page(s) 59-63
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Coffee, Caffeine, UV-visible Spectrophotometer

References
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[2] Nebesny, E., & Grazyna, B., Evaluation of sensory attributes of coffee brews from Robusta coffee roasted under different conditions. Eur. Food Res, 2006; 224, 159–165.
[3] Abrar S., & Nugussie M., Evaluation of coffee quality of Tigray Regional State, Ethiopia (Case study Raya Azebo District). Prudence Journal of Agricultural Research, 2013; 1(2), 3-6.
[4] Behailu W., Abrar S., Nugussie M., &Solomon E., Coffee processing and quality research in Ethiopia, 2008.
[5] Wanyika H. N., Gatebe E. G., Gitu L. M., Ngumba E. K., & Maritim C. W. Determination of caffeine content of tea and instant coffee brands found in the Kenyan market African. Journal of Food Science, 2010; 4(6), 353–358.
[6] Ogah C. O., & Obebe O.T, Caffeine content of cocoa and coffee beverages in Lagos, Nigeria. Journal of innovative research in engineering and sciences, 2012; 3(1), 404-411.
[7] Kapil K., Sumit K., & Yoti M., Estimation of caffeine in different beverages by Ultraviolet Spectroscopy. Int. J. of Pharm. & Life Sci. (IJPLS), 2011; 2(11), 1214-1215.
[8] Forshed J., Erlandsson B., & Jacobsson P., Quantification of aldehydes impurities in poloxamer by 1H NMR spectroscopy. Analytica Chimica Acta, 2005; 552, 160-165.
[9] Tadelech A., & Gholap A. V., Characterization of caffeine and determination of caffeine in tea leave using Uv-visible spectrometer. African Journal of Pure and Applied Chemistry. 2011; 5(1), 1-8.
[10] Kalra K., Kumar S., & Maithani J., Estimation of Caffeine in different beverages by Ultraviolet Spectroscopy. International Journal of Pharmacy and Life Sciences, 2011; 2 (11), 1214-1215.
[11] Smith AP., Caffeine at Work. Hum Psychopharmacology, 2005; 20(6), 441.
[12] Rogers P., & Dernoncourt C., Regular caffeine consumption: a balance of adverse and beneficial effects for mood and psychomotor performance. Pharmacol Biochem Behav, 1998; 59(4), 1039-45.
[13] Stanley D., Passmore R., & Brock J., Human nutrition and dietetics. Longman publishers, 1979; 2 (11): 200.
[14] Mei M., Mohammed I., Badawi A., & Abdalla A., Determination of Caffeine in Some Sudanese Beverages by High Performance Liquid Chromatography. Pakistan Journal of Nutrition, 2012; 11 (4), 336-342.
[15] Thompson R., & Keene K., The pros and cons of caffeine. The Psychologist (The British Psychological Society), 2004; 17 (12), 698–701.
[16] Bibby scientific. The quantitative determination of caffeine in beverages and soft drinks using UV wavelength spectroscopy.jenwayhelp@bibby-scientific.com. www.jenway.com
Cite This Article
  • APA Style

    Zewdu Tadesse Wondimkun, Abdisa Gebisa Jebessa, Liboro Hundito Molloro, Tesfaye Haile. (2016). The Determination of Caffeine Level of Wolaita Zone, Ethiopia Coffee Using UV-visible Spectrophotometer. American Journal of Applied Chemistry, 4(2), 59-63. https://doi.org/10.11648/j.ajac.20160402.14

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    ACS Style

    Zewdu Tadesse Wondimkun; Abdisa Gebisa Jebessa; Liboro Hundito Molloro; Tesfaye Haile. The Determination of Caffeine Level of Wolaita Zone, Ethiopia Coffee Using UV-visible Spectrophotometer. Am. J. Appl. Chem. 2016, 4(2), 59-63. doi: 10.11648/j.ajac.20160402.14

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    AMA Style

    Zewdu Tadesse Wondimkun, Abdisa Gebisa Jebessa, Liboro Hundito Molloro, Tesfaye Haile. The Determination of Caffeine Level of Wolaita Zone, Ethiopia Coffee Using UV-visible Spectrophotometer. Am J Appl Chem. 2016;4(2):59-63. doi: 10.11648/j.ajac.20160402.14

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  • @article{10.11648/j.ajac.20160402.14,
      author = {Zewdu Tadesse Wondimkun and Abdisa Gebisa Jebessa and Liboro Hundito Molloro and Tesfaye Haile},
      title = {The Determination of Caffeine Level of Wolaita Zone, Ethiopia Coffee Using UV-visible Spectrophotometer},
      journal = {American Journal of Applied Chemistry},
      volume = {4},
      number = {2},
      pages = {59-63},
      doi = {10.11648/j.ajac.20160402.14},
      url = {https://doi.org/10.11648/j.ajac.20160402.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajac.20160402.14},
      abstract = {Coffee is an important commodity culturally, commercially and economically in the world. Because of its consumption in most countries in the world, it is important to investigate the exact amounts of its chemical constituents. Caffeine is one of the main components of coffee that affect the quality of coffee. In this research, rapid, simple, accurate, reproducible and cheap UV-Vis spectrometer was used for the quantification of caffeine in coffee and dichloromethane was used as a solvent for the coffee samples taken from Wolaita zone of five woredas. The results of this research show that the amount of caffeine of the coffee samples ranged from (450.13 to 579.60 (mg/L). The amounts of caffeine in Bolso Bomba coffee is the greatest, of all the other coffee samples studied in Wolaita Zone. On the other hand, Kindo Koisha and that of Bolso sore coffee has the least caffeine content compared to the other coffee samples measured. The caffeine content of Sodo Zuria coffee and Humbo coffee samples has an average amounts of caffeine.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - The Determination of Caffeine Level of Wolaita Zone, Ethiopia Coffee Using UV-visible Spectrophotometer
    AU  - Zewdu Tadesse Wondimkun
    AU  - Abdisa Gebisa Jebessa
    AU  - Liboro Hundito Molloro
    AU  - Tesfaye Haile
    Y1  - 2016/03/23
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ajac.20160402.14
    DO  - 10.11648/j.ajac.20160402.14
    T2  - American Journal of Applied Chemistry
    JF  - American Journal of Applied Chemistry
    JO  - American Journal of Applied Chemistry
    SP  - 59
    EP  - 63
    PB  - Science Publishing Group
    SN  - 2330-8745
    UR  - https://doi.org/10.11648/j.ajac.20160402.14
    AB  - Coffee is an important commodity culturally, commercially and economically in the world. Because of its consumption in most countries in the world, it is important to investigate the exact amounts of its chemical constituents. Caffeine is one of the main components of coffee that affect the quality of coffee. In this research, rapid, simple, accurate, reproducible and cheap UV-Vis spectrometer was used for the quantification of caffeine in coffee and dichloromethane was used as a solvent for the coffee samples taken from Wolaita zone of five woredas. The results of this research show that the amount of caffeine of the coffee samples ranged from (450.13 to 579.60 (mg/L). The amounts of caffeine in Bolso Bomba coffee is the greatest, of all the other coffee samples studied in Wolaita Zone. On the other hand, Kindo Koisha and that of Bolso sore coffee has the least caffeine content compared to the other coffee samples measured. The caffeine content of Sodo Zuria coffee and Humbo coffee samples has an average amounts of caffeine.
    VL  - 4
    IS  - 2
    ER  - 

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Author Information
  • Department of Chemistry, College of Natural and Computational Science, Debre Berhan University, Debre Berhan, Ethiopia

  • Department of Chemistry, College of Natural and Computational Science, Debre Berhan University, Debre Berhan, Ethiopia

  • Department of Chemistry, College of Natural and Computational Science, Debre Berhan University, Debre Berhan, Ethiopia

  • Department of Chemistry, College of Natural and Computational Science, Wolaita Sodo University, Wolaita Sodo, Ethiopia

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