| Peer-Reviewed

LC-MS and NMR Based Structural Characterization and Isotopic Abundance Ratio Analysis of Magnesium Gluconate Treated with the Consciousness Energy Healing

Received: 24 February 2017     Accepted: 8 March 2017     Published: 1 April 2017
Views:       Downloads:
Abstract

Magnesium gluconate is widely used pharmaceutical/nutraceutical compound for the prevention and treatment of magnesium deficiency diseases. The present study was designed to explore the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® - Energy of Consciousness Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The LC-MS analysis of both the control and Biofield Energy Treated samples indicated the presence of mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.52 min and fragmentation pattern of both samples were almost identical. The relative peak intensities of the fragment ions were significantly altered in the treated sample compared to the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra of the control and treated samples were found same. The percentage change in the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) was significantly decreased in the treated sample by 48.87% compared to the control sample. Subsequently, the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) in the treated sample was significantly increased by 29.18% compared with the control sample. In summary, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly altered compared with the control sample. Thus, The Trivedi Effect® Treated magnesium gluconate might be helpful to design the novel potent enzyme inhibitors using its kinetic isotope effects. Consequently, The Trivedi Effect® Treated magnesium gluconate would be valuable for designing better pharmaceutical and/or nutraceutical formulations through its altered physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections.

Published in Advances in Bioscience and Bioengineering (Volume 5, Issue 2)
DOI 10.11648/j.abb.20170502.11
Page(s) 22-31
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Magnesium Gluconate, The Trivedi Effect®, Consciousness Energy Healing Treatment, Biofield Energy Healing Treatment, Isotope Effects, Biofield Energy Healers, LC-MS, Isotopic Abundance

References
[1] Ramachandran S, Fontanille P, Pandey A, Larroche C (2006) Gluconic acid: Properties, applications and microbial production. Food Technol Biotechnol 44: 185-195.
[2] Heaton FW (1990) Role of magnesium in enzyme systems in metal ions in biological systems, In: Sigel H, Sigel A (Eds.), Volume 26: Compendium on magnesium and its role in biology, nutrition and physiology, Marcel Dekker Inc., New York.
[3] Garfinkel L, Garfinkel D (1985) Magnesium regulation of the glycolytic pathway and the enzymes involved. Magnesium 4:60-72.
[4] Swaminathan R (2003) Magnesium metabolism and its disorders. Clin Biochem Rev 24:47-66.
[5] Gröber U, Schmidt J, Kisters K (2015) Magnesium in prevention and therapy. Nutrients 7:8199-8226.
[6] William JH, Danziger J (2016) Magnesium deficiency and proton-pump inhibitor use: A clinical review. J Clin Pharmacol 56:660-668.
[7] Guerrera MP, Volpe SL, Mao JJ (2009) Therapeutic uses of magnesium. Am Fam Physician 80:157-162.
[8] Coudray C, Rambeau M, Feillet-Coudray C, Gueux E, Tressol JC, Mazur A, Rayssiguier Y (2005) Study of magnesium bioavailability from ten organic and inorganic Mg salts in Mg-depleted rats using a stable isotope approach. Magnes Res 18:215-223.
[9] Fleming TE, Mansmann Jr HC (1999) Methods and compositions for the prevention and treatment of diabetes mellitus. United States Patent 5871769, 1-10.
[10] Fleming TE, Mansmann Jr HC (1999) Methods and compositions for the prevention and treatment of immunological disorders, inflammatory diseases and infections. United States Patent 5939394, 1-11.
[11] Weglicki WB (2000) Intravenous magnesium gluconate for treatment of conditions caused by excessive oxidative stress due to free radical distribution. United States Patent 6100297, 1-6.
[12] Turner RJ, Dasilva KW, O'Connor C, van den Heuvel C, Vink R (2004) Magnesium gluconate offers no more protection than magnesium sulphate following diffuse trau-matic braininjury in rats. J Am Coll Nutr 23: 541S-544S.
[13] Martin RW, Martin JN Jr, Pryor JA, Gaddy DK, Wiser WL, Morrison JC (1988) Comparison of oral ritodrine and magnesium gluconate for ambulatory tocolysis. Am J Obstet Gynecol 158:1440-1445.
[14] Lee KH, Chung SH, Song JH, Yoon JS, Lee J, Jung MJ, Kim JH (2013) Cosmetic compositions for skin-tightening and method of skin-tightening using the same. United States Patent 8580741 B2.
[15] Stenger VJ (1999) Bioenergetic fields. Sci Rev Alternative Med 3.
[16] Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of biofield treatment on ginseng and organic blueberry yield. Agrivita 35:22-29.
[17] Hammerschlag R, Jain S, Baldwin AL, Gronowicz G, Lutgendor SK, Oschman JL, Yount GL (2012) Biofield research: A roundtable discussion of scientific and methodological issues. J Altern Complement Med 18: 1081-1086.
[18] Rubik B (2002) The biofield hypothesis: Its biophysical basis and role in medicine. J Altern Complement Med 8:703-717.
[19] Koithan M (2009) Introducing complementary and alternative therapies. J Nurse Pract 5:18-20.
[20] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Morphological characterization, quality, yield and DNA fingerprinting of biofield energy treated alphonso mango (Mangifera indica L.). Journal of Food and Nutrition Sciences 3:245-250.
[21] Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Agronomic characteristics, growth analysis, and yield response of biofield treated mustard, cowpea, horse gram, and groundnuts. International Journal of Genetics and Genomics 3:74-80.
[22] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Effect of biofield treated energized water on the growth and health status in chicken (Gallus gallus domesticus). Poult Fish Wildl Sci 3:140.
[23] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Antibiogram of biofield-treated Shigella boydii: Global burden of infections. Science Journal of Clinical Medicine 4:121-126.
[24] Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) An effect of biofield treatment on multidrug-resistant Burkholderia cepacia: A multihost pathogen. J Trop Dis 3:167.
[25] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The potential impact of biofield treatment on human brain tumor cells: A time-lapse video microscopy. J Integr Oncol 4:141.
[26] Trivedi MK, Branton A, Trivedi D, Nayak G, Mishra RK, Jana S (2015) Physicochemical evaluation of biofield treated peptone and malmgren modified terrestrial orchid medium. American Journal of Bioscience and Bioengineering 3:169-177.
[27] Trivedi MK, Branton A, Trivedi D, Nayak G, Singh R, Jana S (2015) Physicochemical characterization of biofield treated orchid maintenance/replate medium. Journal of Plant Sciences 3:285-293.
[28] Trivedi MK, Nayak G, Tallapragada RM, Patil S, Latiyal O, Jana S (2015) Effect of biofield treatment on structural and morphological properties of silicon carbide. J Powder Metall Min 4:132.
[29] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Evaluation of atomic, physical and thermal properties of tellurium powder: Impact of biofield energy treatment. J Electr Electron Syst 4:162.
[30] Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Spectroscopic characterization of chloramphenicol and tetracycline: An impact of biofield. Pharm Anal Acta 6:395.
[31] Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Spectroscopic characterization of biofield treated metronidazole and tinidazole. Med chem 5:340-344.
[32] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Potential impact of biofield treatment on atomic and physical characteristics of magnesium. Vitam Miner 3:129.
[33] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Mishra RK, Jana S (2015) Biofield treatment: A potential strategy for modification of physical and thermal properties of gluten hydrolysate and ipomoea macroelements. J Nutr Food Sci 5:414.
[34] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Mishra RK, Jana S (2015) Characterization of physical, spectroscopic and thermal properties of biofield treated biphenyl. American Journal of Chemical Engineering. 3:58-65.
[35] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Mishra RK, Jana S (2015) Characterization of physical and thermal properties of biofield treated neopentyl glycol. Pharm Anal Chem Open Access 6:101.
[36] MK, Branton A, Trivedi D, Nayak G, Saikia G, Jana S (2015) Mass Spectrometry Analysis of isotopic abundance of 13C, 2H, or 15N in biofield energy treated aminopyridine derivatives. American Journal of Physical Chemistry 4:65-70.
[37] Trivedi MK, Branton A, Trivedi D, Nayak G, Saikia G, Jana S (2015) Quantitative determination of isotopic abundance ratio of 13C, 2H, and 18O in biofield energy treated ortho and meta toluic acid isomers. American Journal of Applied Chemistry 3:217-223.
[38] Trivedi MK, Branton A, Trivedi D, Nayak G, Sethi KK, Jana S (2016) Isotopic abundance ratio analysis of biofield energy treated indole using gas chromatography-mass spectrometry. Science Journal of Chemistry 4:41-48.
[39] Trivedi MK, Branton A, Trivedi D, Nayak G, Panda P, Jana S (2016) Evaluation of the isotopic abundance ratio in biofield energy treated resorcinol using gas chromatography-mass spectrometry technique. Pharm Anal Acta 7:481.
[40] Schellekens RC, Stellaard F, Woerdenbag HJ, Frijlink HW, Kosterink JG (2011) Applications of stable isotopes in clinical pharmacology. Br J Clin Pharmacol 72:879-897.
[41] Muccio Z, Jackson GP (2009) Isotope ratio mass spectrometry. Analyst 134: 13-222.
[42] Vanhaecke F, Kyser K (2012) Isotopic composition of the elements In Isotopic Analysis: Fundamentals and applications using ICP-MS (1stedn), Edited by Vanhaecke F, Degryse P. Wiley-VCH GmbH & Co. KGaA, Weinheim.
[43] Smith RM (2004) Understanding Mass Spectra: A Basic Approach, Second Edition, John Wiley & Sons, Inc, ISBN 0-471-42949-X.
[44] Meija J, Coplen TB, Berglund M, Brand WA, De Bievre P, Groning M, Holden NE, Irrgeher J, Loss RD, Walczyk T, Prohaska T (2016) Isotopic compositions of the elements 2013 (IUPAC technical Report). Pure Appl Chem 88:293-306.
[45] Asperger S (2003) Chemical Kinetics and Inorganic Reaction Mechanisms Springer science + Business media, New York.
[46] Trivedi MK, Mohan TRR (2016) Biofield energy signals, energy transmission and neutrinos. American Journal of Modern Physics 5:172-176.
[47] Cleland WW (2003) The use of isotope effects to determine enzyme mechanisms. J Biol Chem 278:51975-51984.
[48] Nikolic VD, Illic DP, Nikolic LB, Stanojevic LP, Cakic MD, Tacic AD, Ilic-Stojanovic SS (2014) The synthesis and characterization of iron (II) gluconate. Advanced technologies 3:16-24.
Cite This Article
  • APA Style

    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, et al. (2017). LC-MS and NMR Based Structural Characterization and Isotopic Abundance Ratio Analysis of Magnesium Gluconate Treated with the Consciousness Energy Healing. Advances in Bioscience and Bioengineering, 5(2), 22-31. https://doi.org/10.11648/j.abb.20170502.11

    Copy | Download

    ACS Style

    Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; William Dean Plikerd, et al. LC-MS and NMR Based Structural Characterization and Isotopic Abundance Ratio Analysis of Magnesium Gluconate Treated with the Consciousness Energy Healing. Adv. BioSci. Bioeng. 2017, 5(2), 22-31. doi: 10.11648/j.abb.20170502.11

    Copy | Download

    AMA Style

    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, et al. LC-MS and NMR Based Structural Characterization and Isotopic Abundance Ratio Analysis of Magnesium Gluconate Treated with the Consciousness Energy Healing. Adv BioSci Bioeng. 2017;5(2):22-31. doi: 10.11648/j.abb.20170502.11

    Copy | Download

  • @article{10.11648/j.abb.20170502.11,
      author = {Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and William Dean Plikerd and Peter L. Surguy and Robert John Kock and Rolando Baptista Piedad and Russell Phillip Callas and Sakina A. Ansari and Sandra Lee Barrett and Sara Friedman and Steven Lee Christie and Su-Mei Chen Liu and Susan Elizabeth Starling and Susan Jones and Susan Mardis Allen and Susanne Kathrin Wasmus and Terry Ann Benczik and Thomas Charles Slade and Thomas Orban and Victoria L. Vannes and Victoria Margot Schlosser and Yusif Sarkis Yamin Albino and Parthasarathi Panda and Kalyan Kumar Sethi and Snehasis Jana},
      title = {LC-MS and NMR Based Structural Characterization and Isotopic Abundance Ratio Analysis of Magnesium Gluconate Treated with the Consciousness Energy Healing},
      journal = {Advances in Bioscience and Bioengineering},
      volume = {5},
      number = {2},
      pages = {22-31},
      doi = {10.11648/j.abb.20170502.11},
      url = {https://doi.org/10.11648/j.abb.20170502.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20170502.11},
      abstract = {Magnesium gluconate is widely used pharmaceutical/nutraceutical compound for the prevention and treatment of magnesium deficiency diseases. The present study was designed to explore the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® - Energy of Consciousness Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The LC-MS analysis of both the control and Biofield Energy Treated samples indicated the presence of mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.52 min and fragmentation pattern of both samples were almost identical. The relative peak intensities of the fragment ions were significantly altered in the treated sample compared to the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra of the control and treated samples were found same. The percentage change in the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) was significantly decreased in the treated sample by 48.87% compared to the control sample. Subsequently, the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) in the treated sample was significantly increased by 29.18% compared with the control sample. In summary, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly altered compared with the control sample. Thus, The Trivedi Effect® Treated magnesium gluconate might be helpful to design the novel potent enzyme inhibitors using its kinetic isotope effects. Consequently, The Trivedi Effect® Treated magnesium gluconate would be valuable for designing better pharmaceutical and/or nutraceutical formulations through its altered physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections.},
     year = {2017}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - LC-MS and NMR Based Structural Characterization and Isotopic Abundance Ratio Analysis of Magnesium Gluconate Treated with the Consciousness Energy Healing
    AU  - Mahendra Kumar Trivedi
    AU  - Alice Branton
    AU  - Dahryn Trivedi
    AU  - Gopal Nayak
    AU  - William Dean Plikerd
    AU  - Peter L. Surguy
    AU  - Robert John Kock
    AU  - Rolando Baptista Piedad
    AU  - Russell Phillip Callas
    AU  - Sakina A. Ansari
    AU  - Sandra Lee Barrett
    AU  - Sara Friedman
    AU  - Steven Lee Christie
    AU  - Su-Mei Chen Liu
    AU  - Susan Elizabeth Starling
    AU  - Susan Jones
    AU  - Susan Mardis Allen
    AU  - Susanne Kathrin Wasmus
    AU  - Terry Ann Benczik
    AU  - Thomas Charles Slade
    AU  - Thomas Orban
    AU  - Victoria L. Vannes
    AU  - Victoria Margot Schlosser
    AU  - Yusif Sarkis Yamin Albino
    AU  - Parthasarathi Panda
    AU  - Kalyan Kumar Sethi
    AU  - Snehasis Jana
    Y1  - 2017/04/01
    PY  - 2017
    N1  - https://doi.org/10.11648/j.abb.20170502.11
    DO  - 10.11648/j.abb.20170502.11
    T2  - Advances in Bioscience and Bioengineering
    JF  - Advances in Bioscience and Bioengineering
    JO  - Advances in Bioscience and Bioengineering
    SP  - 22
    EP  - 31
    PB  - Science Publishing Group
    SN  - 2330-4162
    UR  - https://doi.org/10.11648/j.abb.20170502.11
    AB  - Magnesium gluconate is widely used pharmaceutical/nutraceutical compound for the prevention and treatment of magnesium deficiency diseases. The present study was designed to explore the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® - Energy of Consciousness Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The LC-MS analysis of both the control and Biofield Energy Treated samples indicated the presence of mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.52 min and fragmentation pattern of both samples were almost identical. The relative peak intensities of the fragment ions were significantly altered in the treated sample compared to the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra of the control and treated samples were found same. The percentage change in the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) was significantly decreased in the treated sample by 48.87% compared to the control sample. Subsequently, the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) in the treated sample was significantly increased by 29.18% compared with the control sample. In summary, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly altered compared with the control sample. Thus, The Trivedi Effect® Treated magnesium gluconate might be helpful to design the novel potent enzyme inhibitors using its kinetic isotope effects. Consequently, The Trivedi Effect® Treated magnesium gluconate would be valuable for designing better pharmaceutical and/or nutraceutical formulations through its altered physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections.
    VL  - 5
    IS  - 2
    ER  - 

    Copy | Download

Author Information
  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

  • Sections