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Characterization of Synthesized NANO-encapsulated Drug for Bone Loss on Hind Limb Suspension Rat Model by NMR and Micro-CT

Received: 17 April 2013     Published: 14 June 2014
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Abstract

A formulation of nano-encapsulated enantiomer of (+) promethazine with desired release rate has been synthesized for establish a localized drug delivery system. It was tested on a hind limb suspension (HLS) disuse rat model, and by using a non-destructive Nuclear Magnetic Resonance (NMR) relaxation technique, and micro computed tomography (Micro-CT) analysis technique to qualitatively evaluate the effectiveness of the new bone formations as well as to compare the current commercial anti-bone loss drug Alendeonate. Our studies suggest that nano-encapsulated (+) promethazine in controlled release formulations conjugating bone-targeting functional groups are effective in promoting bone growth in a disuse rat model

Published in Advances in Bioscience and Bioengineering (Volume 1, Issue 1)
DOI 10.11648/j.abb.20130101.11
Page(s) 1-7
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

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Copyright © The Author(s), 2014. Published by Science Publishing Group

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Keywords

NANO-Encapsulation, Bone, NMR, Micro-CT

References
[1] B. L. Riggs and L. J. Melton, III. The prevention and treatment of osteoporosis. N.Engl.J.Med. 327:620-627,8-27-1992.
[2] B. L. Riggs and L. J. Melton, III. The worldwide problem of osteoporosis: insights afforded by epidemiology. Bone. 17:505S-511S,1995.
[3] Grigoriev, V. S. Oganov, A. V. Bakulin, V. V. Poliakov, L. I. Voronin, V. V. Morgun, V. S. Shnaider, L. V. Murashko, V. E. Novikov, A. LeBlanc, and L. Shackelford. Clinical and Psychological Evaluation of Bone Changes Among Astronauts after Long Term Space Flights (Russian). Aviakosmicheskaia I Ekologicheskaia Meditsina. 32:21-25,1998.
[4] LeBlanc, V. Schneider, and L. Shackelford. Bone Mineral and Lean Tissue Loss after Long Duration Spaceflight. Trans.Amer.Soc.Bone Min.Res. 11S:567-1996.
[5] LeBlanc, L. Shackelford, A. Feiveson, and V. Oganov. Bone Loss in Space: Shuttle/Mir Experience and Bed-Rest Counter Measure Program. 1:17-1999.
[6] S. B. Arnaud. J. S. Harper, and M. Navidi. Mineral distribution in rat skeletons after exposure to a microgravity model.J.Gravit.Physiol. 2:115-116,1995.
[7] H. Uludag, T. Gao, G. R. Wohl, D. Kantoci, and R. F. Zernicke. Bone affinity of a bisphosphonate-conjugated protein in vivo. Biotechnol.Prog. 16:1115-1118,2000.
[8] H. Uludag and J. Yang. Targeting systemically administered proteins to bone by bisphosphonate conjugation. Biotechnol.Prog. 18:604-611,2002.
[9] J. E. Wright, L. Zhao, P. Choi, and H. Uludag. Simulating hydroxyapatite binding of bone-seeking bisphosphonates. Adv.Exp.Med.Biol. 553:139-148,2004.
[10] S.A. Gittens, G. Bansal, C. Kucharski, M. Borden, and H. Uludag. Imparting mineral affinity to fetuin by bisphosphonate conjugation: a comparison of three bisphosphonate conjugation schemes. Mol.Pharm. 2:392-406,2005
[11] S. Zhang, J. E. Wright, G. Bansal, P. Cho, and H. Uludag. Cleavage of disulfide-linked fetuin-bisphosphonate conjugates with three physiological thiols. Biomacromolecules. 6:2800-2808,2005.
[12] J. E. Wright, S. A. Gittens, G. Bansal, P. I. Kitov, D. Sindrey, C. Kucharski, and H. Uludag. A comparison of mineral affinity of bisphosphonate-protein conjugates constructed with disulfide and thioether linkages. Biomaterials. 27:769-784,2006.
[13] T.J. Hall, H. Nyugen, M. Schaeublin, M. Michalsky, and M. Missbach. Phenothiazines are potent inhibitors of osteoclastic bone resorption. Gen.Pharmacol. 27:845-848,1996.
[14] E. J. Boland, US patent application 2006/0258650 A1. Phenothiazine enantiomers as agents for the prevention of bone loss.2006
[15] D. P. Gallegos, Munn, K., Smith, D. M., and Stermer, D. L. A NMR Technique for the Analysis of Pore Structure: Application to Materials with Well-Defined Pore Structure, J. Colloid Interface Sci 119 127.1987
[16] C.L.Glaves and Smith D.M. Membrane Pore Structure Analysis Via NMR Spin-Lattice Relaxation Experiments, J. Member. Sci. 46 167. 1989
[17] M.M. Chui, Philips, R.J., and McCarthy, M.Measurement of the Porous Microstructure of Hydrogels by Nuclear Magnetic Resonance, J. Colloid Interface Sci. 174 336.1995
[18] W. E. Kenyon. Petrophysical Principles of Applications of NMR Logging, The Log Analyst, Mar.-Apr. 21.1997
[19] X. Wang, and Ni, Q. 2003. Determination of Cortical Bone Porosity and Pore size Distribution Using a Field NMR Approach, J. Orthop. Res. 21(2): 312-319.
[20] Q. Ni, King, J.D. and Wang, X.Characterization of Human Bone Structure Changes By Low Field NMR, Measurement Science and Technology, 15, 58 – 66. 2004.
[21] P. Fantazzini, Bortolotti, V., Brown, R.J.S., Garavaglia, C., Viola, R., Giavaresi, G.Two 1H-nuclear magnetic resonance methods to measure internal porosity of bone trabeculae: By solid-liquid signal separation and by longitudinal relaxation. Journal of Applied Physics Vol 95, 339-343. 2004.
[22] Q. Ni, and Nicolella, D.P. The Characterization of Human Cortical Bone Microdamage by Nuclear Magnetic Resonance. Measurement Science and Technology, 16, 659-668.2005
[23] Q. Ni, Nyman, J., Wang, X., De Los Santos, A. and Nicolella, D.Assessment of Water Distribution Changes in Human Cortical Bones by Nuclear Magnetic Resonance. Measurement Science and Technology, 18, 715-723.2007
[24] E. R. Morey-Holton, B. P. Halloran, L. P. Garetto, and S. B. Doty. Animal housing influences the response of bone to spaceflight in juvenile rats. J.Appl.Physiol. 88:1303-1309,2000
[25] H.Y. Carr, and Purcell, E. M. Effects of Diffusion on Free Precession in Nuclear Magnetic Resonance Experiments. Phys. Rev. 904, No.3 630. 1954
[26] S. Meiboom, and Gill, D. Modified Spin-Echo Method for Measuring Nuclear Relaxation Times. Rev. Sci. Inst. 29, 688. 1958
[27] K.R. Broenstein and C.E. Tarr. Importance of Classical Difussion in NMR Studies of Water in Biological Cells. Phys Rev. A 19, 2446, 1979.
[28] J. Kluge, F. Fusaro, N. Casas, M. Mazzotti, G. Muhrer. Production of PLGA micro- and nanocomposites by supercritical fluid extraction of emulsions: I. Encapsulation of lysozyme. The Journal of Supercritical Fluids, Volume 50, Issue 3, 327-335, 2009.
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  • APA Style

    Qingwen Ni, Hong Dixon, Gloria Gutierrez, Long Bi, Yi-Xian Qin. (2014). Characterization of Synthesized NANO-encapsulated Drug for Bone Loss on Hind Limb Suspension Rat Model by NMR and Micro-CT. Advances in Bioscience and Bioengineering, 1(1), 1-7. https://doi.org/10.11648/j.abb.20130101.11

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    ACS Style

    Qingwen Ni; Hong Dixon; Gloria Gutierrez; Long Bi; Yi-Xian Qin. Characterization of Synthesized NANO-encapsulated Drug for Bone Loss on Hind Limb Suspension Rat Model by NMR and Micro-CT. Adv. BioSci. Bioeng. 2014, 1(1), 1-7. doi: 10.11648/j.abb.20130101.11

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    AMA Style

    Qingwen Ni, Hong Dixon, Gloria Gutierrez, Long Bi, Yi-Xian Qin. Characterization of Synthesized NANO-encapsulated Drug for Bone Loss on Hind Limb Suspension Rat Model by NMR and Micro-CT. Adv BioSci Bioeng. 2014;1(1):1-7. doi: 10.11648/j.abb.20130101.11

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  • @article{10.11648/j.abb.20130101.11,
      author = {Qingwen Ni and Hong Dixon and Gloria Gutierrez and Long Bi and Yi-Xian Qin},
      title = {Characterization of Synthesized NANO-encapsulated Drug for Bone Loss on Hind Limb Suspension Rat Model by NMR and Micro-CT},
      journal = {Advances in Bioscience and Bioengineering},
      volume = {1},
      number = {1},
      pages = {1-7},
      doi = {10.11648/j.abb.20130101.11},
      url = {https://doi.org/10.11648/j.abb.20130101.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20130101.11},
      abstract = {A formulation of nano-encapsulated enantiomer of (+) promethazine with desired release rate has been synthesized for establish a localized drug delivery system. It was tested on a hind limb suspension (HLS) disuse rat model, and by using a non-destructive Nuclear Magnetic Resonance (NMR) relaxation technique, and micro computed tomography (Micro-CT) analysis technique to qualitatively evaluate the effectiveness of the new bone formations as well as to compare the current commercial anti-bone loss drug Alendeonate. Our studies suggest that nano-encapsulated (+) promethazine in controlled release formulations conjugating bone-targeting functional groups are effective in promoting bone growth in a disuse rat model},
     year = {2014}
    }
    

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    AU  - Qingwen Ni
    AU  - Hong Dixon
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    DO  - 10.11648/j.abb.20130101.11
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    AB  - A formulation of nano-encapsulated enantiomer of (+) promethazine with desired release rate has been synthesized for establish a localized drug delivery system. It was tested on a hind limb suspension (HLS) disuse rat model, and by using a non-destructive Nuclear Magnetic Resonance (NMR) relaxation technique, and micro computed tomography (Micro-CT) analysis technique to qualitatively evaluate the effectiveness of the new bone formations as well as to compare the current commercial anti-bone loss drug Alendeonate. Our studies suggest that nano-encapsulated (+) promethazine in controlled release formulations conjugating bone-targeting functional groups are effective in promoting bone growth in a disuse rat model
    VL  - 1
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Author Information
  • Southwest Research Institute, San Antonio, TX, USA

  • Southwest Research Institute, San Antonio, TX, USA

  • Southwest Research Institute, San Antonio, TX, USA

  • Southwest Research Institute, San Antonio, TX, USA

  • State University of New York at Stony Brook, Stony Brook NY, USA

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