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Phytochemical Screening, Tyrosinase Inhibitory Effects and Kinetics of Cam Wood Dye Extracts

Received: 5 February 2016     Accepted: 18 February 2016     Published: 28 April 2016
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Abstract

Tyrosinase is a key enzyme in melanin biosynthesis, as it catalyzes the oxidation of o-diphenols to o-quinones. Cam wood dye was extracted using methanol, acetone and dichloromethane; while extracts obtained were screened for Phytochemicals as well as their tyrosinase inhibitory effects and kinetic studies. Phytochemical screening of the three extracts showed the presence of total phlobatanins in all extracts except methanolic which had minute presence. Cardiac glycosides and flavonoids were also observed in the dichloromethane extracts and methanolic extract respectively. The methanolic extracts had best enzyme inhibition (84.1%) at highest concentration considered of 400µg/ml as compared to the dichloromethane and acetone extracts with peak values of 58.5 and 51.5% respectively. The enzyme kinetics analysis of substrate showed same inhibition type for three extracts which was non-competitive and its mechanism irreversible. The Michealis-Mentens constants for the three extracts were determined to be 0.344, 0.355 and 0.214mM, for acetone, dichloromethane and methanolic extracts respectively while the values of Vmax/Km shows inhibiting extracts followed the order: methanol extract > acetone extract > dichloromethame extract. The result therefore showed that methanolic extracts of Cam wood dye was the most effective in tyrosinase inhibition.

Published in Advances in Biochemistry (Volume 4, Issue 2)
DOI 10.11648/j.ab.20160402.12
Page(s) 16-20
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Cam Wood Dye, Tyrosinase, Inhibition, Phytochemicals

References
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    David Morakinyo Sanni, Oluwasegun Victor Omotoyinbo. (2016). Phytochemical Screening, Tyrosinase Inhibitory Effects and Kinetics of Cam Wood Dye Extracts. Advances in Biochemistry, 4(2), 16-20. https://doi.org/10.11648/j.ab.20160402.12

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    ACS Style

    David Morakinyo Sanni; Oluwasegun Victor Omotoyinbo. Phytochemical Screening, Tyrosinase Inhibitory Effects and Kinetics of Cam Wood Dye Extracts. Adv. Biochem. 2016, 4(2), 16-20. doi: 10.11648/j.ab.20160402.12

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    AMA Style

    David Morakinyo Sanni, Oluwasegun Victor Omotoyinbo. Phytochemical Screening, Tyrosinase Inhibitory Effects and Kinetics of Cam Wood Dye Extracts. Adv Biochem. 2016;4(2):16-20. doi: 10.11648/j.ab.20160402.12

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  • @article{10.11648/j.ab.20160402.12,
      author = {David Morakinyo Sanni and Oluwasegun Victor Omotoyinbo},
      title = {Phytochemical Screening, Tyrosinase Inhibitory Effects and Kinetics of Cam Wood Dye Extracts},
      journal = {Advances in Biochemistry},
      volume = {4},
      number = {2},
      pages = {16-20},
      doi = {10.11648/j.ab.20160402.12},
      url = {https://doi.org/10.11648/j.ab.20160402.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20160402.12},
      abstract = {Tyrosinase is a key enzyme in melanin biosynthesis, as it catalyzes the oxidation of o-diphenols to o-quinones. Cam wood dye was extracted using methanol, acetone and dichloromethane; while extracts obtained were screened for Phytochemicals as well as their tyrosinase inhibitory effects and kinetic studies. Phytochemical screening of the three extracts showed the presence of total phlobatanins in all extracts except methanolic which had minute presence. Cardiac glycosides and flavonoids were also observed in the dichloromethane extracts and methanolic extract respectively. The methanolic extracts had best enzyme inhibition (84.1%) at highest concentration considered of 400µg/ml as compared to the dichloromethane and acetone extracts with peak values of 58.5 and 51.5% respectively. The enzyme kinetics analysis of substrate showed same inhibition type for three extracts which was non-competitive and its mechanism irreversible. The Michealis-Mentens constants for the three extracts were determined to be 0.344, 0.355 and 0.214mM, for acetone, dichloromethane and methanolic extracts respectively while the values of Vmax/Km shows inhibiting extracts followed the order: methanol extract > acetone extract > dichloromethame extract. The result therefore showed that methanolic extracts of Cam wood dye was the most effective in tyrosinase inhibition.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Phytochemical Screening, Tyrosinase Inhibitory Effects and Kinetics of Cam Wood Dye Extracts
    AU  - David Morakinyo Sanni
    AU  - Oluwasegun Victor Omotoyinbo
    Y1  - 2016/04/28
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ab.20160402.12
    DO  - 10.11648/j.ab.20160402.12
    T2  - Advances in Biochemistry
    JF  - Advances in Biochemistry
    JO  - Advances in Biochemistry
    SP  - 16
    EP  - 20
    PB  - Science Publishing Group
    SN  - 2329-0862
    UR  - https://doi.org/10.11648/j.ab.20160402.12
    AB  - Tyrosinase is a key enzyme in melanin biosynthesis, as it catalyzes the oxidation of o-diphenols to o-quinones. Cam wood dye was extracted using methanol, acetone and dichloromethane; while extracts obtained were screened for Phytochemicals as well as their tyrosinase inhibitory effects and kinetic studies. Phytochemical screening of the three extracts showed the presence of total phlobatanins in all extracts except methanolic which had minute presence. Cardiac glycosides and flavonoids were also observed in the dichloromethane extracts and methanolic extract respectively. The methanolic extracts had best enzyme inhibition (84.1%) at highest concentration considered of 400µg/ml as compared to the dichloromethane and acetone extracts with peak values of 58.5 and 51.5% respectively. The enzyme kinetics analysis of substrate showed same inhibition type for three extracts which was non-competitive and its mechanism irreversible. The Michealis-Mentens constants for the three extracts were determined to be 0.344, 0.355 and 0.214mM, for acetone, dichloromethane and methanolic extracts respectively while the values of Vmax/Km shows inhibiting extracts followed the order: methanol extract > acetone extract > dichloromethame extract. The result therefore showed that methanolic extracts of Cam wood dye was the most effective in tyrosinase inhibition.
    VL  - 4
    IS  - 2
    ER  - 

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Author Information
  • Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria

  • Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria

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