Single molecule atomic resolution real time electron microscopic movie imagine is an emerging new tool for obtaining dynamic structural information on molecule and molecular assemblies. This method provide a hitherto inaccessible pos-sibility to in situ observe the time evolution of chemical events at various temperatures from the beginning till the end, as demonstrated for kinetics study of cycloaddition of fullerene molecules, which was found to occur via an excited State or via radical cation depending on the temperature. One unique feature of this methodology is that, by observing directly the reaction events, one can obtain information on the frequency of events unperturbed by molecular diffusion. With the obtained experimental proof of what the quantum mechanical transition State theory predicted, in that isolated molecules behave as if all their accessible states were occupied in a random order.

SMART -EM Imaging, Molecular Movies, Quantum Mechanical Transition