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Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae)

Received: 22 January 2021     Accepted: 30 January 2021     Published: 10 February 2021
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Abstract

Cordia myxa is a Cameroonian pharmacopoeia traditional plant. It belongs to the Boraginaceae family, which different parts are used in the treatment of various bacterial infections. The phytochemical screening carried out on the Cordia myxa species indicates the presence of alkaloids, triterpenes, steroids, carbohydrates, flavonoids and saponins. Our investigations on the roots of this plant allowed us to isolate two triterpenes from the ethyl acetate extract, which crystallize in the Hex/AcEt [2/8] solvent system; (23R) campesta-9 (11), 24 (30) diene-18,23-diol 3-O-α-rhamnopyranoside (compound 1) and alphitolic acid (compound 2). The isolation and characterization of these compounds were carried out respectively by silica gel chromatography and by NMR (1D, 2D) spectral analysis. Compound 1and 2 were tested on the in vitro growth of E.coli and Salmonella typhi using the Muller-Hinton solid medium disc method. The results revealed that these compounds have a dose-dependent antibacterial activity on Salmonella typhi. However, compound 2 had a better antibacterial potential on the Salmonella strain (18.6±0.6mm, 30mg/ml) with the MIC=0.25mg/ml than the compound 1 (14.5±0.4mm, 30mg/ml,) with the MIC=0.44mg/ml. Few classes of antibiotics have been tested, to look for those with the best results and compare the different doses of our compounds. The two compounds did not have any effect on E. coli.

Published in American Journal of Chemical and Biochemical Engineering (Volume 5, Issue 1)
DOI 10.11648/j.ajcbe.20210501.11
Page(s) 1-7
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Cordia myxa, Antibacterial Effect, Salmonella typhi, Triterpene

References
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    Dabole Bernard, Matcheme Mathieu, Moussa Djaouda, Abdou Tchoukoua, Wakayansam Bouba Romeo, et al. (2021). Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae). American Journal of Chemical and Biochemical Engineering, 5(1), 1-7. https://doi.org/10.11648/j.ajcbe.20210501.11

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    ACS Style

    Dabole Bernard; Matcheme Mathieu; Moussa Djaouda; Abdou Tchoukoua; Wakayansam Bouba Romeo, et al. Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae). Am. J. Chem. Biochem. Eng. 2021, 5(1), 1-7. doi: 10.11648/j.ajcbe.20210501.11

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    AMA Style

    Dabole Bernard, Matcheme Mathieu, Moussa Djaouda, Abdou Tchoukoua, Wakayansam Bouba Romeo, et al. Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae). Am J Chem Biochem Eng. 2021;5(1):1-7. doi: 10.11648/j.ajcbe.20210501.11

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  • @article{10.11648/j.ajcbe.20210501.11,
      author = {Dabole Bernard and Matcheme Mathieu and Moussa Djaouda and Abdou Tchoukoua and Wakayansam Bouba Romeo and Meli Alain and Loura Benoit},
      title = {Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae)},
      journal = {American Journal of Chemical and Biochemical Engineering},
      volume = {5},
      number = {1},
      pages = {1-7},
      doi = {10.11648/j.ajcbe.20210501.11},
      url = {https://doi.org/10.11648/j.ajcbe.20210501.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20210501.11},
      abstract = {Cordia myxa is a Cameroonian pharmacopoeia traditional plant. It belongs to the Boraginaceae family, which different parts are used in the treatment of various bacterial infections. The phytochemical screening carried out on the Cordia myxa species indicates the presence of alkaloids, triterpenes, steroids, carbohydrates, flavonoids and saponins. Our investigations on the roots of this plant allowed us to isolate two triterpenes from the ethyl acetate extract, which crystallize in the Hex/AcEt [2/8] solvent system; (23R) campesta-9 (11), 24 (30) diene-18,23-diol 3-O-α-rhamnopyranoside (compound 1) and alphitolic acid (compound 2). The isolation and characterization of these compounds were carried out respectively by silica gel chromatography and by NMR (1D, 2D) spectral analysis. Compound 1and 2 were tested on the in vitro growth of E.coli and Salmonella typhi using the Muller-Hinton solid medium disc method. The results revealed that these compounds have a dose-dependent antibacterial activity on Salmonella typhi. However, compound 2 had a better antibacterial potential on the Salmonella strain (18.6±0.6mm, 30mg/ml) with the MIC=0.25mg/ml than the compound 1 (14.5±0.4mm, 30mg/ml,) with the MIC=0.44mg/ml. Few classes of antibiotics have been tested, to look for those with the best results and compare the different doses of our compounds. The two compounds did not have any effect on E. coli.},
     year = {2021}
    }
    

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    T1  - Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae)
    AU  - Dabole Bernard
    AU  - Matcheme Mathieu
    AU  - Moussa Djaouda
    AU  - Abdou Tchoukoua
    AU  - Wakayansam Bouba Romeo
    AU  - Meli Alain
    AU  - Loura Benoit
    Y1  - 2021/02/10
    PY  - 2021
    N1  - https://doi.org/10.11648/j.ajcbe.20210501.11
    DO  - 10.11648/j.ajcbe.20210501.11
    T2  - American Journal of Chemical and Biochemical Engineering
    JF  - American Journal of Chemical and Biochemical Engineering
    JO  - American Journal of Chemical and Biochemical Engineering
    SP  - 1
    EP  - 7
    PB  - Science Publishing Group
    SN  - 2639-9989
    UR  - https://doi.org/10.11648/j.ajcbe.20210501.11
    AB  - Cordia myxa is a Cameroonian pharmacopoeia traditional plant. It belongs to the Boraginaceae family, which different parts are used in the treatment of various bacterial infections. The phytochemical screening carried out on the Cordia myxa species indicates the presence of alkaloids, triterpenes, steroids, carbohydrates, flavonoids and saponins. Our investigations on the roots of this plant allowed us to isolate two triterpenes from the ethyl acetate extract, which crystallize in the Hex/AcEt [2/8] solvent system; (23R) campesta-9 (11), 24 (30) diene-18,23-diol 3-O-α-rhamnopyranoside (compound 1) and alphitolic acid (compound 2). The isolation and characterization of these compounds were carried out respectively by silica gel chromatography and by NMR (1D, 2D) spectral analysis. Compound 1and 2 were tested on the in vitro growth of E.coli and Salmonella typhi using the Muller-Hinton solid medium disc method. The results revealed that these compounds have a dose-dependent antibacterial activity on Salmonella typhi. However, compound 2 had a better antibacterial potential on the Salmonella strain (18.6±0.6mm, 30mg/ml) with the MIC=0.25mg/ml than the compound 1 (14.5±0.4mm, 30mg/ml,) with the MIC=0.44mg/ml. Few classes of antibiotics have been tested, to look for those with the best results and compare the different doses of our compounds. The two compounds did not have any effect on E. coli.
    VL  - 5
    IS  - 1
    ER  - 

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Author Information
  • Department of Chemistry, Faculty of science, University of Maroua, Maroua, Cameroon

  • Department of Chemistry, Faculty of science, University of Maroua, Maroua, Cameroon

  • Department of Life and Earth Sciences, Higher Teachers’ Training College, University of Maroua, Maroua, Cameroon

  • Department of Chemistry, Faculty of science, University of Maroua, Maroua, Cameroon

  • Department of Biological Sciences, Faculty of Science, University of Maroua, Maroua, Cameroon

  • Department of Chemistry, Higher Teachers’ Training College, University of Maroua, Maroua, Cameroon

  • Department of Life and Earth Sciences, Higher Teachers’ Training College, University of Maroua, Maroua, Cameroon

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