Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors
International Journal of Clinical Oncology and Cancer Research
Volume 3, Issue 4, August 2018, Pages: 49-54
Received: Aug. 24, 2018;
Accepted: Sep. 14, 2018;
Published: Oct. 17, 2018
Views 1505 Downloads 118
Mohammad Sahajadul Alam, Department of Surgical Oncology, National Institute of Cancer Research and Hospital, Dhaka, Bangladesh
M. Mizanur Rahman, Department of Surgical Oncology, National Institute of Cancer Research and Hospital, Dhaka, Bangladesh
Md. Monowar Hossain, Department of Surgical Oncology, Khulna Medical College, Khulna, Bangladesh
Mohammed Abu Kawsar Sarker, Department of Surgery, Aichi Medical College, Dhaka, Bangladesh
Md. Monzurul Islam, Department of Surgical Oncology, National Institute of Cancer Research and Hospital, Dhaka, Bangladesh
Rokeya Begum, Centre for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka, Bangladesh
Most Umme Habiba, Centre for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka, Bangladesh
Gazi Nurun Nahar Sultana, Centre for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka, Bangladesh
Follow on us
Like all cancers, Gastric cancer (GC) is also known to be of genetic origin. Genes are mutated in every steps of cell division, among them hMLH1 is a DNA mismatch gene which plays important role during carcinogenesis. This study was chalked out to find out the status of mutated hMLH1 Gene and its clinicopathological relevance among Bangladeshi gastric cancer patients. It was a cross sectional study conducted during January 2015 to April 2016. Tissue extracted from gastrectomy specimen of carcinoma stomach patients sent to specified laboratory. In the laboratory after DNA extraction, PCR, sequencing and analysis was done. In this study out of 19, exons 7 and 8 and introns 8 and 9 were studied after primer designing for hMLH1 gene mutation. After analysis, mutated gene were matched with clinicopathological parameters like age, sex, location of tumour, types and grade of tumour to see their relevance. Out of 45 patients, mutation was found in 15 patients (33.3%). Most gene alteration was found in elderly patients, more in male. Antral growth had more (80.0%) mutation and in ulceroproliferative type (66.7%) (p < .005). Mutation was common in intestinal type (73.3%) of GC. hMLH1 gene mutation found more in the moderately differentiated carcinoma patients. Factors like age, sex, morphology, Lauren’s type, grading, personal habits etc. might play pivotal role in the development of gene mutation. Multi-centre large study are required to extract more relevant information in this regard.
Hmlh1 Gene Mutation, Gastric Cancer, Clinicopathological Relevance
To cite this article
Mohammad Sahajadul Alam,
M. Mizanur Rahman,
Md. Monowar Hossain,
Mohammed Abu Kawsar Sarker,
Md. Monzurul Islam,
Most Umme Habiba,
Gazi Nurun Nahar Sultana,
Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors, International Journal of Clinical Oncology and Cancer Research.
Vol. 3, No. 4,
2018, pp. 49-54.
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Ferlay J, Soerjomataram I, Ervik M, Dikshit R, Eser S, Mathers C, et al. Cancer incidence and mortality worldwide: Sources, methods and major Patterns in GLOBOCAN 2012. Int. J. Cancer.136:359-386.
Orditura M, Galizia G, Sforza V, Gambardella V, Fabozzi A, Laterza MM, et al. Treatment of gastric cancer . World Journal of Gastroenterology. 2014 20 (7): 1635–49.
Cabebe EC, Mehta VK, Fisher G, Talavera F, Movsas M, McKenna R, et al. Gastric Cancer. Medscape Reference. WebMD. Archived from the original on 7 April 2014. Retrieved 4 April 2014.
Mathias U, Cheng Z, Sunjae L, Evelina S, Linn F, Gholamreza B, Rui B, Muhammad A, Zhengtao L (2017-08-18). A pathology atlas of the human cancer transcriptome. Science. 357 (6352):
Tahara E. Genetic pathways of two types of gastric cancer. IARC Scientific Publications 2004. 157: 327–349.
Bandla S, Pennathur, Luketich AJD. Comparative genomics of esophageal adenocarcinoma and squamous cell carcinoma. Annals of Thoracic Surgery 2012. 93:1101–1106
Smith MG, Hold GL, Tahara E & El-Omar EM. Cellular and molecular aspect of gastric cancer. World Journal of Gastroenterology 2006. 12: 2979–299.
Ong CAJ, Lao-Sirieix P & Fitzgerald. Biomarkers in Barrett's esophagus and esophageal adenocarcinoma: predictors of progression and prognosis. World Journal of Gastroenterology 2010. 16:5669–5681.
Hanahan D, Weinberg R. The Hallmarks of Cancer. Cell 2000. 100: 57-70.
Nagini S. Carcinoma of the stomach: A review of epidemiology, pathogenesis, molecular genetics and chemoprevention. World J Gastrointestinal Oncology 2012. 4:156-169.
Lauren P. The two histological main types of gastric carcinoma: Diffuse and so-called intestinal-type carcinoma, An attempt at a histo-clinical classification. Acta Pathol Microbiol Scand 1965. 64:31–49.
Tamura G. Alterations of tumour suppressor and tumour-related genes in the development and progression of gastric cancer. World J Gastroenterology 2006. 12: 192–8.
Wenxian Z, Binshuang X, Lifeng W, Nong X, Qiong H, Yaping W, et. al. The MLH1 2101C>A (Q701K) variant increases the risk of gastric cancer in Chinese males. BMC Gastroenterology 2011. 11:133.
Available at https://ghr.nlm.nih.gov/gene/MLH1 accessed on 22.02.2018
Xian-Qiu X, Wei-Da G, Shi-Zhi W, Zheng-Dong Z, Xiao-Ping R, Feng R et.al. Polymorphisms of mismatch repair gene hMLH1 and hMSH2 and risk of gastric cancer in a Chinese population. Oncology Letters 2012. 3: 591-598.
Ping L, Xiao-Yong Z, Yun S, Dao-Fu Z. Microsatellite instability in gastric cancer and pre-cancerous Lesions. World Journal of Gastroenterology 2005; 11: 4904-4907.
Wirtz HC, Muller W, Noguchi T, Scheven M, Ruschoff J & Gabbert HE, et al. Prognostic value and clinicopathological profile of microsatellite instability in gastric cancer. Clin Cancer Res 1998. 4:1749-1754.
Hudler P, Voulk K, Liovic M, Repse S, Juvan R, Komel R. Mutations in the hMLH1 gene in Slovenian patients with gastric carcinoma. Clin Genet 2004: 65: 405–411.
Peltomaki P. DNA mismatch repair and cancer. Mutat Res 2001: 488: 77–85.