The Role of Morus Nigra Extract and Its Active Compounds as Drug Candidate on Human Colorectal Adenocarcinoma Cell Line HT-29
International Journal of Clinical Oncology and Cancer Research
Volume 2, Issue 1, February 2017, Pages: 10-14
Received: Jan. 15, 2017;
Accepted: Feb. 3, 2017;
Published: Mar. 1, 2017
Views 1364 Downloads 85
Ece Çakıroğlu, Department of Basic Oncology, Institute of Oncology, Dokuz Eylul University, Izmir, Turkey
Tuğba Uysal, Department of Basic Oncology, Institute of Oncology, Dokuz Eylul University, Izmir, Turkey
Gizem Çalıbaşı Koçal, Department of Basic Oncology, Institute of Oncology, Dokuz Eylul University, Izmir, Turkey; Personalized Medicine and Pharmacogenomics/Genomics Research Centre-BIFAGEM, Dokuz Eylul University, Izmir, Turkey
Fatih Aygenli, Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey
Gülin Baran, Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey
Yasemin Baskın, Department of Basic Oncology, Institute of Oncology, Dokuz Eylul University, Izmir, Turkey; Personalized Medicine and Pharmacogenomics/Genomics Research Centre-BIFAGEM, Dokuz Eylul University, Izmir, Turkey; Department of Medical Informatics and Biostatistics, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey
Follow on us
According to laboratory-based in vitro researches, there are numerous medicinal plants and natural compounds that indicate potential as an anticancer agent. Morus nigra (M.nigra) (black mulberry) and its active components are strong candidates to be anticancer agents. The purpose of the present study was to investigate antiproliferative and antimigratory effects of M.nigra extract, its leptin Morniga G (MorG) and one of its component Chalcone 4 hydrate; and their synergistic effect in combination with cetuximab application on colorectal adenocarcinoma cell line HT-29. The antiproliferative effect was determined by impedance based proliferation assay following exposure to M.nigra extract (10%, 1%, 0.1%), MorG (0.5, 5, 50 µM) and Chalcone 4 hydrate (0.5, 5, 50 µM) for 48 hours. The antimigratory effect of M.nigra extract and its coponents, was investigated by a wound-healing assay for 48 h. According to results, Morus nigra extract and its leptin MorG reduced cell viability. After 48 hours of 200 µg/ml Cetuximab exposure with M.nigra extract and MorG at different concentrations, a significant decrease on the cell viability was detected when compared to Cetuximab application. MorG can be suggested as a potantial conjugate for targeted drug. However, futher studies are required to fully understand its mechanisms of action.
Morus Nigra, Morniga G, Chalcone 4 Hydrate, Colorectal Adenocarcinoma, HT-29
To cite this article
Gizem Çalıbaşı Koçal,
The Role of Morus Nigra Extract and Its Active Compounds as Drug Candidate on Human Colorectal Adenocarcinoma Cell Line HT-29, International Journal of Clinical Oncology and Cancer Research.
Vol. 2, No. 1,
2017, pp. 10-14.
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Gallagher DJ, Kemeny N (2010). Metastatic colorectal cancer: from improved survival to potential cure. Oncology 78: 237–248.
Hosseini A, Ghorbani A (2015). Cancer therapy with phytochemicals: evidence from clinical studies. Avicenna J Phytomed 5 (2): 84-97.
Minko T (2004). Drug targeting to the colon with lectins and neoglycoconjugates. Adv Drug Deliv Rev 56 (4): 491-509.
L.-L. Fu, X. Zhao, H.-L. Xu, X. Wen, S.-Y. Wang, Liu B, Bao JK, Wei YQ (2012). Identification of microRNA-regulated autophagic pathways in plant lectin induced cancer cell death. Cell Prolif. 445: 477–485.
Singh T, Wu JH, Peumans WJ, Rouge P, Van Damme EJ, Wu AM (2007). Recognition profile of Morus nigra agglutinin (Morniga G) expressed by monomeric ligands, simple clusters and mammalian polyvalent glycotopes. Mol Immunol 44: 451–462.
Wei H, Zhu JJ, Liu XQ, Feng WH, Wang ZM, Yan LH (2016). Review of bioactive compounds from root barks of Morus plants (Sang-Bai-Pi) and their pharmacological effects. Cogent Chemistry 2: 1212320.
Qi Z, Liu M, Liu Y, Zhang M, Yang G (2014). Tetramethoxychalcone, a chalcone derivative, suppresses proliferation, blocks cell cycle progression, and induces apoptosis of human ovarian cancer cells. PLoS One. 9 (9): e106206.
Drutovic D, Chripkova M, Pilatova M, Kruzliak P, Perjesi P, Sarissky M, Lupi M, Damia G, Broggini M, Mojzis J (2014). Benzylidenetetralones, cyclic chalcone analogues, induce cell cycle arrest and apoptosis in HCT116 colorectal cancer cells. Tumour Biol. 35 (10): 9967-9975.
Sigmaaldrich.com. (2016). Chalcone 4 hydrate ≥98% (HPLC) | Sigma-Aldrich. [online] Available at: http://www.sigmaaldrich.com/catalog/product/sigma/c7870?lang=en®ion=TR.
Kryczek I, Wei S, Keller E, Liu R, Zou W (2007). Stroma-derived factor (SDF-1/CXCL12) and human tumor pathogenesis. Am J Physiol Cell Physiol 292: C987-C995.
Aydin S, Yilmaz Ö, Gökçe Z (2011). Effectiveness of matured Morus nigra L. (black mulberry) fruit extract on 2,2-diphenyl-1-picrylhydrazyl (DPPH●) and hydroxyl (OH●) radicals as compared to less matured fruit extract. Afr. J. Biotechnol 10 (71): 16037-16044.
Qadir MI, Ali M, Ibrahim Z (2014). Anticancer activity of Morus nigra leaves extract. Bangladesh J Pharmacol 9: 496-497.
Poiroux G, Pitié M, Culerrier R, Lafont E, Ségui B, Van Damme EJ, Peumans WJ, Bernadou J, Levade T, Roug é P, et al (2011). Targeting of T/Tn Antigens with a Plant Lectin to Kill Human Leukemia Cells by Photochemotherapy. PLoS One. 6 (8): e23315.
Takahashi M, Takara Ki Toyozato T, Wada K (2012). A novel bioactive chalcone of Morus australis inhibits tyrosinase activity and melanin biosynthesis in B16 melanoma cells. J Oleo Sci 61 (10): 5855-92.
Liu, B, Li, C. Y., Bian, H. J., Min, M. W., Chen, L. F., Bao, J. K (2009). Antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A on human melanoma A375 cells. Arch. Biochem. Biophys 482: 1–6.