To determine the Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of chitosan from cockroach and cricket against bacterial isolates, Staphylococccus aureus, Staphylococcus epidermidis, Klebsiella pneumoniae, Streptococcus pyogenes, Bacillus subtilis and Pseudomonas aeruginosa. The antimicrobial efficacy of chitosan was determined by the standard method of Clinical and Laboratory Standards Institute (CLSI). Different concentrations were prepared and the MICs were calculated by tube dilution method in triplicates. The MBCs were determined by the lowest concentration that kills 99.9% of the initial bacterial population. The data generated were analyzed by ANOVA and Turkeys post hoc test using spss soft ware. The MIC of cockroach chitosan against S. aureus, B. subtilis, K. pneumoniae, S. pyogenes, P. aeruginosa and S. epidermidis, were found to be 240, 220, 0, 260, 240 and 0mg/ml while the MIC of cricket chitosan against the organisms were found to be 240,240,240,0, 220 and 280mg/ml. The MBC of chitosan from cockroach against the organisms were found to be 260, 260, 0, 280, 260 and 0 mg/ml while that of cricket were found to be 240, 240, 280, 0, 240 and 300 mg/ml. This study revealed that chitosan from P. Americana and A. domestica can be used as disinfectants in wounds, since they act against S. aureus, B. subtilis, K. pneumoniae, S. pyogenes, P. aeruginosa and S. epidermidis which are organisms commonly found in wound.
| Published in | International Journal of Microbiology and Biotechnology (Volume 7, Issue 4) |
| DOI | 10.11648/j.ijmb.20220704.14 |
| Page(s) | 177-182 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Chitosan, Antimicrobial Activity, Bacterial Isolates, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC)
| [1] | Azam, A., Muhammed, R., Khorasgarin, S., Vaezifar, Fateli, R., Habibollah, Y. and Maryam, K. (2016). Evaluation of antibacterial efficacy of chitosan and chitosan nano particles on carcinogenic streptococci: an invitro study. Iranian Journal of Microbiology, 8 (2): 93-100. |
| [2] | Brook, I. (2013). Penicillin failure in the treatment of Streptococcal pharyngo tonsillitis. Current Infectious Diseases Reports, 15 (3): 232-235. |
| [3] | Chandran, R., Williams, L., Hung, A., Nowlin, K., Lajeunesse, D. (2016). SEM and Characterization of anatomic variation in chitin organization in insects and arthropod cuticles. Micron, 82, 74-85. |
| [4] | Clinical and Laboratory Standards institute (2020). M100:- Performance for antimicrobial susceptibility testing. Wayne, PA, 30th edition CLSI guideline AVI016. |
| [5] | Hall, F., Liceaga, A., Johnson, P. E. (2018). Effect of enzymatic hydrolysis on bioactive properties and allergenicity of cricket (Gryllodes sigillatus) protein. Journal of Food Chemistry, 262, 39-47. |
| [6] | Hugo, S. B. and Rusel, A. D. (2003). Pharmaceutical microbiology, 6th edition, Blackwell scientific publishers, Oxford, London pp: 91-129. |
| [7] | Ibitoye, E. B., Lokman, I. H., Hezmee, M. N. M., Goh, Y. M., Zuki, A. B. and Jimoh, A. A. (2018). Extraction and physiochemical characterization of chitin and chitosan isolated from house cricket. Journal of Biomedical Materials, 13. |
| [8] | Ibitoye, B. E., Ghazali, U. M., Adekunle, J. B., Uwazie, J. N. and Ajiboye, T. O. (2017). Antidyslipidemic, anti-inflammatory and antioxidant activities of aqeous leaf extract of Dioscoreophyllum cumminsii (Stapf) diels in high fat diet fed rats. Evidence Based Complementary and Alternative Medicine, 8. |
| [9] | Jianhui, L. and Shaoling, Z. (2020). Antibacterial activities of chitosan and its derivatives and their interaction mechanism with bacteria: current state and perspectives. European Polymer Journal, volume 38. |
| [10] | Jose, R., Armendariz, V. and Herrera, I. (2004). Size controlled gold nanoparticles formation by Avenasativa biomass: use of plants in nanobiotech. Journal of Nanoparticle Research, 6, 377-382. |
| [11] | Kamal, M., Adly, E., Alharbi, S. A., Khaleed, A. S., Rady, M. H., Ibrahim, N. A. (2020). Exploring simplified methods for insect chitin extraction and application as a potential alternative bioethanol source. Insects, 11, 778. |
| [12] | Kumar, D. and Verma, A. P. (2014). Isolation and degree of deacetylation of chitin from cultured biomass of diatoms. Bionotes, 14 (4), pp 116-117. |
| [13] | Lee, K. S., Yun, E. Y., Goo, T. W. (2020). Antimicrobial activity of an extract of Hermatia illucens larvae immunized with Lactobacillus casei against Salmonella species. Insects, 11, 707. |
| [14] | Li, K., Xing, R.., Liu, S. and Li, P. (2016). Advances in preparation, analysis and biological activities of single chitooligosaccharides. Carbohydrate Polymers, 139, 178-190. |
| [15] | Li, X., He, Z., Ding, W. F., Zhang, X., Ma, C. J. and Feng, Y. (2020). Assessment of dermal safety of oil extracted from P. americana acute dermal toxicity, irritation and sensitization. Cutaneous Ocul Toxicology, 39, 193-199. |
| [16] | Liceaga, A. M. (2019). Approaches for utilizing inscet protein for human consumption: effect of enzymatic hydrolysis on protein quality and functionality. Annual Entomology Society Am, 112, 529-532. |
| [17] | Lipsky, B. A. (2017). Evidence- based antibiotic therapy of diabetic foot infections. FEMS immunology Medical microbiology. 26 (3-4) pp 267-276. |
| [18] | Mahmoud, K., Eslam, A., Sulaiman, A. A., Amany, S. K., Magda, H. R. and Nevin, A. I. (2020). Exploring simplified methods for insect chitin extraction and applcationas a potential alternative bioethanol resource. Journal of Insects, 11, 0788. |
| [19] | Mohan, K., Ganesan, A. R., Muralisankar, R., Sathishkumar, P., Uthayakumar, V., Chandirasekar, R., Revathi, N. (2020). Recent insights in to the extraction, characterization and bioactives of chitin and chitosan from insects. Trends in Food Science and Technology, 105, 17-42. |
| [20] | Mosaheb, M., Khan, N. A., Siddiqui, R. (2018). Cockroaches, locusts and envenomating arthropods: a promising source of antimicrobials. Iran Journal of Basic Medical Sciences, 21, 873-877. |
| [21] | Muhammed, M., Mylonakis, E., Podsiadlowski, L. and Vilcinskas, A. (2016). Diversity, evolution and medical applicatons of insect antimicrobial peptides. Philosophical Transactions Royal Society B, 371: 20150290. |
| [22] | Narguess, H. M., Emtithal, A., Taher, S., Gamal, R. S. and Ahmed H. M. E. (2015). Isolation and Characterization of Chitosan from different local Insects in Egypt. International Journal of Biological Macromolecules, 82, 871-877. |
| [23] | Nkechinyere, M. O. K and Chioma, O. O. (2020). Antibacterial activities and phytochemical analysis of chromolaena odorata leaves on MRSA. American Jornal of Biomedical and Life Sciences. Vol8. No2 pp33-40. |
| [24] | Nordstorm, K., Riesbeck, K. and Schaar, V. (2014). Group A Streptococci are protected from amoxicillin-mediated killing by vesicles containing beta-lactamase derived from Haemophilus influenzae. Journal of Antimicrobial Chemotherapy, 69 (1): 117-120. |
| [25] | Nguyen, T., Chen, X., Chai, J., Li, R., Han, X., Chen, X., Liu, S., Chen, M., Xu, X. (2020). Antipyretic, anti-inflammatory and analgesic activities of P. americana extract and underlying mechanisms. Biomedical Pharmacotherapy, 123, 109753. |
| [26] | Prashik, P., Jayant, P., Sandeep, M., Rahul, M. and Smita, D. (2020). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus. Biomaterial Investigations in Dentistry, 7 (1): 105-109. |
| [27] | Rawda M. B. and Hadeer I. M., (2015). Chitin extraction, composition of different six Insects species and their comparable characteristics with that of the Shrimp. Journal of American Science, 11 (6), 127- 134. |
| [28] | Rinaudo, M. (2016). Chitin and chitosan: properties and applications. Progammed Polymer Science, 31, 603-632. |
| [29] | Samanthi, U., Wickramarachchi, S., Wijeratne, K. and Paranagama, P. (2017). Two new antioxidant active polyketides from Penicillum citrinum, an endolichenic fungus isolated from Parmotrema species in Sri lanka. Journal of the National Science Foundation of Sri lanka, 43 (2): 119-126. |
| [30] | Sonia, H. and Koushic, M. D. (2020). Isolation and extraction of chitosan from shrimp shells. International Journal of Advanced Research, 31. |
| [31] | Umar, Z. G., Abalaka, M. E., Daniyan, S. Y., Babayi, H and Adeniyi, K. A. (2022). Physicochemical and biometabolite Characterizations of chitosan from American cockroach (Periplanata americana) and cricket (Acheta domesticus). BIOMED Natural and Applied Science, 2 (1): 25-36. |
| [32] | Wenming, X., Qing, L., Wei, W., Peixin, X. (2002). Preparation and antimicrobial activity of water soluble chitosan derivative. Journal of Carbohydrate Polymers, 50 (1): 35-40. |
| [33] | World Health Organization, WHO (2012). Global Malaria Programme. World malaria report 2012. Geneva: The Organization; 2012. p. 30–3. |
| [34] | World Health Organisation, WHO (2017). Chronnic diseases and their common risk factors. Geneva The Organization. p. 30–3. |
APA Style
Umar Zainab Garba, Abalaka Moses Enemaduku, Daniyan Safiya Yahaya, Babayi Hausatu. (2022). Determination of the MIC and MBC of Chitosan from Cockroach and Cricket Against Some Bacterial Isolates. International Journal of Microbiology and Biotechnology, 7(4), 177-182. https://doi.org/10.11648/j.ijmb.20220704.14
ACS Style
Umar Zainab Garba; Abalaka Moses Enemaduku; Daniyan Safiya Yahaya; Babayi Hausatu. Determination of the MIC and MBC of Chitosan from Cockroach and Cricket Against Some Bacterial Isolates. Int. J. Microbiol. Biotechnol. 2022, 7(4), 177-182. doi: 10.11648/j.ijmb.20220704.14
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ijmb.20220704.14,
author = {Umar Zainab Garba and Abalaka Moses Enemaduku and Daniyan Safiya Yahaya and Babayi Hausatu},
title = {Determination of the MIC and MBC of Chitosan from Cockroach and Cricket Against Some Bacterial Isolates},
journal = {International Journal of Microbiology and Biotechnology},
volume = {7},
number = {4},
pages = {177-182},
doi = {10.11648/j.ijmb.20220704.14},
url = {https://doi.org/10.11648/j.ijmb.20220704.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220704.14},
abstract = {To determine the Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of chitosan from cockroach and cricket against bacterial isolates, Staphylococccus aureus, Staphylococcus epidermidis, Klebsiella pneumoniae, Streptococcus pyogenes, Bacillus subtilis and Pseudomonas aeruginosa. The antimicrobial efficacy of chitosan was determined by the standard method of Clinical and Laboratory Standards Institute (CLSI). Different concentrations were prepared and the MICs were calculated by tube dilution method in triplicates. The MBCs were determined by the lowest concentration that kills 99.9% of the initial bacterial population. The data generated were analyzed by ANOVA and Turkeys post hoc test using spss soft ware. The MIC of cockroach chitosan against S. aureus, B. subtilis, K. pneumoniae, S. pyogenes, P. aeruginosa and S. epidermidis, were found to be 240, 220, 0, 260, 240 and 0mg/ml while the MIC of cricket chitosan against the organisms were found to be 240,240,240,0, 220 and 280mg/ml. The MBC of chitosan from cockroach against the organisms were found to be 260, 260, 0, 280, 260 and 0 mg/ml while that of cricket were found to be 240, 240, 280, 0, 240 and 300 mg/ml. This study revealed that chitosan from P. Americana and A. domestica can be used as disinfectants in wounds, since they act against S. aureus, B. subtilis, K. pneumoniae, S. pyogenes, P. aeruginosa and S. epidermidis which are organisms commonly found in wound.},
year = {2022}
}
TY - JOUR T1 - Determination of the MIC and MBC of Chitosan from Cockroach and Cricket Against Some Bacterial Isolates AU - Umar Zainab Garba AU - Abalaka Moses Enemaduku AU - Daniyan Safiya Yahaya AU - Babayi Hausatu Y1 - 2022/12/08 PY - 2022 N1 - https://doi.org/10.11648/j.ijmb.20220704.14 DO - 10.11648/j.ijmb.20220704.14 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 177 EP - 182 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20220704.14 AB - To determine the Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of chitosan from cockroach and cricket against bacterial isolates, Staphylococccus aureus, Staphylococcus epidermidis, Klebsiella pneumoniae, Streptococcus pyogenes, Bacillus subtilis and Pseudomonas aeruginosa. The antimicrobial efficacy of chitosan was determined by the standard method of Clinical and Laboratory Standards Institute (CLSI). Different concentrations were prepared and the MICs were calculated by tube dilution method in triplicates. The MBCs were determined by the lowest concentration that kills 99.9% of the initial bacterial population. The data generated were analyzed by ANOVA and Turkeys post hoc test using spss soft ware. The MIC of cockroach chitosan against S. aureus, B. subtilis, K. pneumoniae, S. pyogenes, P. aeruginosa and S. epidermidis, were found to be 240, 220, 0, 260, 240 and 0mg/ml while the MIC of cricket chitosan against the organisms were found to be 240,240,240,0, 220 and 280mg/ml. The MBC of chitosan from cockroach against the organisms were found to be 260, 260, 0, 280, 260 and 0 mg/ml while that of cricket were found to be 240, 240, 280, 0, 240 and 300 mg/ml. This study revealed that chitosan from P. Americana and A. domestica can be used as disinfectants in wounds, since they act against S. aureus, B. subtilis, K. pneumoniae, S. pyogenes, P. aeruginosa and S. epidermidis which are organisms commonly found in wound. VL - 7 IS - 4 ER -
Information
Email: