Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance

Huanhuan Jiang; iaqin Li; Honghuo He; Minjia Zhang; Liang Yunyan; Fan Jingyu; Wang Jingyi; Mo Cuiting; Sainan Li; Na Lv Yi

doi:doi:10.11648/j.ijmb.20220704.13

Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance

Huanhuan Jiang, iaqin Li, Honghuo He, Minjia Zhang, Liang Yunyan, Fan Jingyu, Wang Jingyi, Mo Cuiting, Sainan Li, Na Lv Yi

Published in International Journal of Microbiology and Biotechnology (Volume 7, Issue 4)

Received: 26 October 2022 Accepted: 10 November 2022 Published: 23 November 2022

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Abstract

Phosphate-solubilizing microorganisms (PSMs) are able to dissolve insoluble phosphorus in the soil. They play an important role in promoting soil nutrient cycling, plant growth, and dissolving insoluble phosphorus in the soil, and are therefore important microbial resources in biological fertilizers. In this study, the PSMs in rice rhizosphere soil were isolated and purified. Twenty-one phosphate-solubilizing strains were screened, including 6 bacteria and 15 fungi. Morphological analysis and molecular identification of the phosphate-solubilizing bacteria were conducted to determine the species. According to 16S rRNA molecular identification, the phosphate-solubilizing bacteria belonged to Pseudomonas aeruginosa, Bacillus albus, Bacillus subtilis, and Rhizobium sp. The phosphate-solubilizing ability of the PSMs was determined using a plate containing tricalcium phosphate (TCP). The results showed that the phosphate-solubilizing index of most of the PSMs increased first and then decreased. The phosphate-solubilizing ability of the PSMs toward the inorganic phosphate TCP, iron phosphate, aluminum phosphate, and organophosphorus of lecithin was measured using the liquid method. The results showed that the phosphate-solubilizing ability of the PSMs was 0.39mg/L–494.13mg/L, and the phosphate-solubilizing ability of TCP was the best. Among them, 13 strains had phosphate-solubilizing ability above 100 mg/L. In this study, Twenty-one strains of highly efficient phosphate-solubilizing bacteria were isolated, providing strain resources and a theoretical reference for the development of phosphate-solubilizing biological fertilizer.

Published in	International Journal of Microbiology and Biotechnology (Volume 7, Issue 4)
DOI	10.11648/j.ijmb.20220704.13
Page(s)	169-176
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Phosphate-Solubilizing Microorganisms, Rice Rhizosphere Soil, Phosphate-Solubilizing Ability

References

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APA Style

Huanhuan Jiang, iaqin Li, Honghuo He, Minjia Zhang, Liang Yunyan, et al. (2022). Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance. International Journal of Microbiology and Biotechnology, 7(4), 169-176. https://doi.org/10.11648/j.ijmb.20220704.13

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ACS Style

Huanhuan Jiang; iaqin Li; Honghuo He; Minjia Zhang; Liang Yunyan, et al. Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance. Int. J. Microbiol. Biotechnol. 2022, 7(4), 169-176. doi: 10.11648/j.ijmb.20220704.13

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AMA Style

Huanhuan Jiang, iaqin Li, Honghuo He, Minjia Zhang, Liang Yunyan, et al. Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance. Int J Microbiol Biotechnol. 2022;7(4):169-176. doi: 10.11648/j.ijmb.20220704.13

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@article{10.11648/j.ijmb.20220704.13,
  author = {Huanhuan Jiang and iaqin Li and Honghuo He and Minjia Zhang and Liang Yunyan and Fan Jingyu and Wang Jingyi and Mo Cuiting and Sainan Li and Na Lv Yi},
  title = {Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {7},
  number = {4},
  pages = {169-176},
  doi = {10.11648/j.ijmb.20220704.13},
  url = {https://doi.org/10.11648/j.ijmb.20220704.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220704.13},
  abstract = {Phosphate-solubilizing microorganisms (PSMs) are able to dissolve insoluble phosphorus in the soil. They play an important role in promoting soil nutrient cycling, plant growth, and dissolving insoluble phosphorus in the soil, and are therefore important microbial resources in biological fertilizers. In this study, the PSMs in rice rhizosphere soil were isolated and purified. Twenty-one phosphate-solubilizing strains were screened, including 6 bacteria and 15 fungi. Morphological analysis and molecular identification of the phosphate-solubilizing bacteria were conducted to determine the species. According to 16S rRNA molecular identification, the phosphate-solubilizing bacteria belonged to Pseudomonas aeruginosa, Bacillus albus, Bacillus subtilis, and Rhizobium sp. The phosphate-solubilizing ability of the PSMs was determined using a plate containing tricalcium phosphate (TCP). The results showed that the phosphate-solubilizing index of most of the PSMs increased first and then decreased. The phosphate-solubilizing ability of the PSMs toward the inorganic phosphate TCP, iron phosphate, aluminum phosphate, and organophosphorus of lecithin was measured using the liquid method. The results showed that the phosphate-solubilizing ability of the PSMs was 0.39mg/L–494.13mg/L, and the phosphate-solubilizing ability of TCP was the best. Among them, 13 strains had phosphate-solubilizing ability above 100 mg/L. In this study, Twenty-one strains of highly efficient phosphate-solubilizing bacteria were isolated, providing strain resources and a theoretical reference for the development of phosphate-solubilizing biological fertilizer.},
 year = {2022}
}

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TY  - JOUR
T1  - Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance
AU  - Huanhuan Jiang
AU  - iaqin Li
AU  - Honghuo He
AU  - Minjia Zhang
AU  - Liang Yunyan
AU  - Fan Jingyu
AU  - Wang Jingyi
AU  - Mo Cuiting
AU  - Sainan Li
AU  - Na Lv Yi
Y1  - 2022/11/23
PY  - 2022
N1  - https://doi.org/10.11648/j.ijmb.20220704.13
DO  - 10.11648/j.ijmb.20220704.13
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 169
EP  - 176
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20220704.13
AB  - Phosphate-solubilizing microorganisms (PSMs) are able to dissolve insoluble phosphorus in the soil. They play an important role in promoting soil nutrient cycling, plant growth, and dissolving insoluble phosphorus in the soil, and are therefore important microbial resources in biological fertilizers. In this study, the PSMs in rice rhizosphere soil were isolated and purified. Twenty-one phosphate-solubilizing strains were screened, including 6 bacteria and 15 fungi. Morphological analysis and molecular identification of the phosphate-solubilizing bacteria were conducted to determine the species. According to 16S rRNA molecular identification, the phosphate-solubilizing bacteria belonged to Pseudomonas aeruginosa, Bacillus albus, Bacillus subtilis, and Rhizobium sp. The phosphate-solubilizing ability of the PSMs was determined using a plate containing tricalcium phosphate (TCP). The results showed that the phosphate-solubilizing index of most of the PSMs increased first and then decreased. The phosphate-solubilizing ability of the PSMs toward the inorganic phosphate TCP, iron phosphate, aluminum phosphate, and organophosphorus of lecithin was measured using the liquid method. The results showed that the phosphate-solubilizing ability of the PSMs was 0.39mg/L–494.13mg/L, and the phosphate-solubilizing ability of TCP was the best. Among them, 13 strains had phosphate-solubilizing ability above 100 mg/L. In this study, Twenty-one strains of highly efficient phosphate-solubilizing bacteria were isolated, providing strain resources and a theoretical reference for the development of phosphate-solubilizing biological fertilizer.
VL  - 7
IS  - 4
ER  -

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Author Information

Huanhuan Jiang

School of Life Sciences, Zhaoqing University, Zhaoqing, China
iaqin Li

School of Life Sciences, Zhaoqing University, Zhaoqing, China
Honghuo He

School of Life Sciences, Zhaoqing University, Zhaoqing, China
Minjia Zhang

School of Life Sciences, Zhaoqing University, Zhaoqing, China
Liang Yunyan

School of Life Sciences, Zhaoqing University, Zhaoqing, China
Fan Jingyu

School of Life Sciences, Zhaoqing University, Zhaoqing, China
Wang Jingyi

School of Life Sciences, Zhaoqing University, Zhaoqing, China
Mo Cuiting

School of Life Sciences, Zhaoqing University, Zhaoqing, China
Sainan Li

School of Life Sciences, Zhaoqing University, Zhaoqing, China
Na Lv Yi

School of Life Sciences, Zhaoqing University, Zhaoqing, China

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APA Style

Huanhuan Jiang, iaqin Li, Honghuo He, Minjia Zhang, Liang Yunyan, et al. (2022). Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance. International Journal of Microbiology and Biotechnology, 7(4), 169-176. https://doi.org/10.11648/j.ijmb.20220704.13

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ACS Style

Huanhuan Jiang; iaqin Li; Honghuo He; Minjia Zhang; Liang Yunyan, et al. Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance. Int. J. Microbiol. Biotechnol. 2022, 7(4), 169-176. doi: 10.11648/j.ijmb.20220704.13

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AMA Style

Huanhuan Jiang, iaqin Li, Honghuo He, Minjia Zhang, Liang Yunyan, et al. Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance. Int J Microbiol Biotechnol. 2022;7(4):169-176. doi: 10.11648/j.ijmb.20220704.13

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@article{10.11648/j.ijmb.20220704.13,
  author = {Huanhuan Jiang and iaqin Li and Honghuo He and Minjia Zhang and Liang Yunyan and Fan Jingyu and Wang Jingyi and Mo Cuiting and Sainan Li and Na Lv Yi},
  title = {Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {7},
  number = {4},
  pages = {169-176},
  doi = {10.11648/j.ijmb.20220704.13},
  url = {https://doi.org/10.11648/j.ijmb.20220704.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220704.13},
  abstract = {Phosphate-solubilizing microorganisms (PSMs) are able to dissolve insoluble phosphorus in the soil. They play an important role in promoting soil nutrient cycling, plant growth, and dissolving insoluble phosphorus in the soil, and are therefore important microbial resources in biological fertilizers. In this study, the PSMs in rice rhizosphere soil were isolated and purified. Twenty-one phosphate-solubilizing strains were screened, including 6 bacteria and 15 fungi. Morphological analysis and molecular identification of the phosphate-solubilizing bacteria were conducted to determine the species. According to 16S rRNA molecular identification, the phosphate-solubilizing bacteria belonged to Pseudomonas aeruginosa, Bacillus albus, Bacillus subtilis, and Rhizobium sp. The phosphate-solubilizing ability of the PSMs was determined using a plate containing tricalcium phosphate (TCP). The results showed that the phosphate-solubilizing index of most of the PSMs increased first and then decreased. The phosphate-solubilizing ability of the PSMs toward the inorganic phosphate TCP, iron phosphate, aluminum phosphate, and organophosphorus of lecithin was measured using the liquid method. The results showed that the phosphate-solubilizing ability of the PSMs was 0.39mg/L–494.13mg/L, and the phosphate-solubilizing ability of TCP was the best. Among them, 13 strains had phosphate-solubilizing ability above 100 mg/L. In this study, Twenty-one strains of highly efficient phosphate-solubilizing bacteria were isolated, providing strain resources and a theoretical reference for the development of phosphate-solubilizing biological fertilizer.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Isolation of Phosphate-Solubilizing Microorganisms Naturally Colonizing the Rice Rhizosphere and Evaluation of Their Phosphate-Solubilizing Performance
AU  - Huanhuan Jiang
AU  - iaqin Li
AU  - Honghuo He
AU  - Minjia Zhang
AU  - Liang Yunyan
AU  - Fan Jingyu
AU  - Wang Jingyi
AU  - Mo Cuiting
AU  - Sainan Li
AU  - Na Lv Yi
Y1  - 2022/11/23
PY  - 2022
N1  - https://doi.org/10.11648/j.ijmb.20220704.13
DO  - 10.11648/j.ijmb.20220704.13
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 169
EP  - 176
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20220704.13
AB  - Phosphate-solubilizing microorganisms (PSMs) are able to dissolve insoluble phosphorus in the soil. They play an important role in promoting soil nutrient cycling, plant growth, and dissolving insoluble phosphorus in the soil, and are therefore important microbial resources in biological fertilizers. In this study, the PSMs in rice rhizosphere soil were isolated and purified. Twenty-one phosphate-solubilizing strains were screened, including 6 bacteria and 15 fungi. Morphological analysis and molecular identification of the phosphate-solubilizing bacteria were conducted to determine the species. According to 16S rRNA molecular identification, the phosphate-solubilizing bacteria belonged to Pseudomonas aeruginosa, Bacillus albus, Bacillus subtilis, and Rhizobium sp. The phosphate-solubilizing ability of the PSMs was determined using a plate containing tricalcium phosphate (TCP). The results showed that the phosphate-solubilizing index of most of the PSMs increased first and then decreased. The phosphate-solubilizing ability of the PSMs toward the inorganic phosphate TCP, iron phosphate, aluminum phosphate, and organophosphorus of lecithin was measured using the liquid method. The results showed that the phosphate-solubilizing ability of the PSMs was 0.39mg/L–494.13mg/L, and the phosphate-solubilizing ability of TCP was the best. Among them, 13 strains had phosphate-solubilizing ability above 100 mg/L. In this study, Twenty-one strains of highly efficient phosphate-solubilizing bacteria were isolated, providing strain resources and a theoretical reference for the development of phosphate-solubilizing biological fertilizer.
VL  - 7
IS  - 4
ER  -

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