Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon

Cedric Gueguim; Vanessa Kenny Megam; Serge Damase Oyong Assiene; Pulcherie Ngo Lend; Marthe Viviane Fonkam; Edouard OrlineToukap; Aurelle Yondo; Lucien Honore Etame Sone

doi:doi:10.11648/j.ijmb.20220704.12

Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon

Cedric Gueguim, Vanessa Kenny Megam, Serge Damase Oyong Assiene, Pulcherie Ngo Lend, Marthe Viviane Fonkam, Edouard OrlineToukap, Aurelle Yondo, Lucien Honore Etame Sone

Published in International Journal of Microbiology and Biotechnology (Volume 7, Issue 4)

Received: 31 August 2022 Accepted: 16 September 2022 Published: 21 October 2022

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Abstract

Urinary tract infections, usually caused by bacteria that originate from the intestinal flora or the perineal flora, constitute a major public health problem for Africa in general, especially in Cameroon. Known failures with empiric therapy are becoming increasingly problematic. The same is proven true for the consistent bacterial resistance to antibiotics. The purpose of this study was to determine the frequency of Gram-negative bacterial strains isolated from urine samples at Laquintinie Hospital of Douala and to establish their antibiotic resistance profile. Over a period of three months, four hundred and thirty-six (436) urine samples were taken and analyzed at the bacteriology laboratory of the Laquintinie hospital in Douala. The Bacteria were isolated according to standard methods and identification using the API20E gallery. The antibiotic sensitivity profile of the isolated bacteria was done by the disk diffusion method according to the recommendations of the Antibiogram Committee of the French Society of Microbiology 2018. One hundred and fifty-one (151) bacterial strains were isolated, mostly from women (58.67%) with a male or female (M/F) ratio of 0.7. We noted a predominance of Escherichia coli strains (58.28%), followed by Klebsiella Pneumoniae (22.51%), Proteus mirabilis (4.64%), Pseudomonas aeruginosa (3.97%), Enterobacter cloacae (2.65%), Acinetobacter (1.98%), Enterobacter sakazakii (1.32%) and the rest 4.65%. All the bacterial strains isolated showed strong resistance to beta-lactam (greater than 50%) but were predominantly susceptible to Imipenem (96.39%) and Amikacin (90.47%). Escherichia coli was particularly resistant to beta-lactams such as amoxicillin (87.5%), amoxicillin + clavulanic acid (85.24%), Cefuroxime (65.57%), Cefixime (54.83%) and Ceftriaxone (52.70%). The bacterial strains of Klebsiella pneumoniae obtained a total resistance to amoxicillin (100%), clavulanic acid + amoxicillin (69.23%), Cefixime (68.42%), Ceftriaxone (52.70%), and Cefuroxime (52.00%). Escherichia coli (58.28%) and Klebsiella pneumoniae (22.51%) are the two main bacterial species that are the most isolated. Resistance of Escherichia coli and Klebsiella pneumoniae to antibiotics is a growing phenomenon. Monitoring for bacterial resistance to antibiotics is imperative in our context. Develop more effective prevention strategies, screening, prophylactic isolation.

Published in	International Journal of Microbiology and Biotechnology (Volume 7, Issue 4)
DOI	10.11648/j.ijmb.20220704.12
Page(s)	163-168
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Urinary Tract Infection, Gram-Negative Bacilli, Antibiotic, Sensitivity Profile

References

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Cedric Gueguim, Vanessa Kenny Megam, Serge Damase Oyong Assiene, Pulcherie Ngo Lend, Marthe Viviane Fonkam, et al. (2022). Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon. International Journal of Microbiology and Biotechnology, 7(4), 163-168. https://doi.org/10.11648/j.ijmb.20220704.12

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ACS Style

Cedric Gueguim; Vanessa Kenny Megam; Serge Damase Oyong Assiene; Pulcherie Ngo Lend; Marthe Viviane Fonkam, et al. Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon. Int. J. Microbiol. Biotechnol. 2022, 7(4), 163-168. doi: 10.11648/j.ijmb.20220704.12

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AMA Style

Cedric Gueguim, Vanessa Kenny Megam, Serge Damase Oyong Assiene, Pulcherie Ngo Lend, Marthe Viviane Fonkam, et al. Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon. Int J Microbiol Biotechnol. 2022;7(4):163-168. doi: 10.11648/j.ijmb.20220704.12

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@article{10.11648/j.ijmb.20220704.12,
  author = {Cedric Gueguim and Vanessa Kenny Megam and Serge Damase Oyong Assiene and Pulcherie Ngo Lend and Marthe Viviane Fonkam and Edouard OrlineToukap and Aurelle Yondo and Lucien Honore Etame Sone},
  title = {Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {7},
  number = {4},
  pages = {163-168},
  doi = {10.11648/j.ijmb.20220704.12},
  url = {https://doi.org/10.11648/j.ijmb.20220704.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220704.12},
  abstract = {Urinary tract infections, usually caused by bacteria that originate from the intestinal flora or the perineal flora, constitute a major public health problem for Africa in general, especially in Cameroon. Known failures with empiric therapy are becoming increasingly problematic. The same is proven true for the consistent bacterial resistance to antibiotics. The purpose of this study was to determine the frequency of Gram-negative bacterial strains isolated from urine samples at Laquintinie Hospital of Douala and to establish their antibiotic resistance profile. Over a period of three months, four hundred and thirty-six (436) urine samples were taken and analyzed at the bacteriology laboratory of the Laquintinie hospital in Douala. The Bacteria were isolated according to standard methods and identification using the API20E gallery. The antibiotic sensitivity profile of the isolated bacteria was done by the disk diffusion method according to the recommendations of the Antibiogram Committee of the French Society of Microbiology 2018. One hundred and fifty-one (151) bacterial strains were isolated, mostly from women (58.67%) with a male or female (M/F) ratio of 0.7. We noted a predominance of Escherichia coli strains (58.28%), followed by Klebsiella Pneumoniae (22.51%), Proteus mirabilis (4.64%), Pseudomonas aeruginosa (3.97%), Enterobacter cloacae (2.65%), Acinetobacter (1.98%), Enterobacter sakazakii (1.32%) and the rest 4.65%. All the bacterial strains isolated showed strong resistance to beta-lactam (greater than 50%) but were predominantly susceptible to Imipenem (96.39%) and Amikacin (90.47%). Escherichia coli was particularly resistant to beta-lactams such as amoxicillin (87.5%), amoxicillin + clavulanic acid (85.24%), Cefuroxime (65.57%), Cefixime (54.83%) and Ceftriaxone (52.70%). The bacterial strains of Klebsiella pneumoniae obtained a total resistance to amoxicillin (100%), clavulanic acid + amoxicillin (69.23%), Cefixime (68.42%), Ceftriaxone (52.70%), and Cefuroxime (52.00%). Escherichia coli (58.28%) and Klebsiella pneumoniae (22.51%) are the two main bacterial species that are the most isolated. Resistance of Escherichia coli and Klebsiella pneumoniae to antibiotics is a growing phenomenon. Monitoring for bacterial resistance to antibiotics is imperative in our context. Develop more effective prevention strategies, screening, prophylactic isolation.},
 year = {2022}
}

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TY  - JOUR
T1  - Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon
AU  - Cedric Gueguim
AU  - Vanessa Kenny Megam
AU  - Serge Damase Oyong Assiene
AU  - Pulcherie Ngo Lend
AU  - Marthe Viviane Fonkam
AU  - Edouard OrlineToukap
AU  - Aurelle Yondo
AU  - Lucien Honore Etame Sone
Y1  - 2022/10/21
PY  - 2022
N1  - https://doi.org/10.11648/j.ijmb.20220704.12
DO  - 10.11648/j.ijmb.20220704.12
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 163
EP  - 168
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20220704.12
AB  - Urinary tract infections, usually caused by bacteria that originate from the intestinal flora or the perineal flora, constitute a major public health problem for Africa in general, especially in Cameroon. Known failures with empiric therapy are becoming increasingly problematic. The same is proven true for the consistent bacterial resistance to antibiotics. The purpose of this study was to determine the frequency of Gram-negative bacterial strains isolated from urine samples at Laquintinie Hospital of Douala and to establish their antibiotic resistance profile. Over a period of three months, four hundred and thirty-six (436) urine samples were taken and analyzed at the bacteriology laboratory of the Laquintinie hospital in Douala. The Bacteria were isolated according to standard methods and identification using the API20E gallery. The antibiotic sensitivity profile of the isolated bacteria was done by the disk diffusion method according to the recommendations of the Antibiogram Committee of the French Society of Microbiology 2018. One hundred and fifty-one (151) bacterial strains were isolated, mostly from women (58.67%) with a male or female (M/F) ratio of 0.7. We noted a predominance of Escherichia coli strains (58.28%), followed by Klebsiella Pneumoniae (22.51%), Proteus mirabilis (4.64%), Pseudomonas aeruginosa (3.97%), Enterobacter cloacae (2.65%), Acinetobacter (1.98%), Enterobacter sakazakii (1.32%) and the rest 4.65%. All the bacterial strains isolated showed strong resistance to beta-lactam (greater than 50%) but were predominantly susceptible to Imipenem (96.39%) and Amikacin (90.47%). Escherichia coli was particularly resistant to beta-lactams such as amoxicillin (87.5%), amoxicillin + clavulanic acid (85.24%), Cefuroxime (65.57%), Cefixime (54.83%) and Ceftriaxone (52.70%). The bacterial strains of Klebsiella pneumoniae obtained a total resistance to amoxicillin (100%), clavulanic acid + amoxicillin (69.23%), Cefixime (68.42%), Ceftriaxone (52.70%), and Cefuroxime (52.00%). Escherichia coli (58.28%) and Klebsiella pneumoniae (22.51%) are the two main bacterial species that are the most isolated. Resistance of Escherichia coli and Klebsiella pneumoniae to antibiotics is a growing phenomenon. Monitoring for bacterial resistance to antibiotics is imperative in our context. Develop more effective prevention strategies, screening, prophylactic isolation.
VL  - 7
IS  - 4
ER  -

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Author Information

Cedric Gueguim

Department of Biology, Higher Institute of Medical Technology, Yaounde, Cameroon
Vanessa Kenny Megam

School of Health Sciences, Catholic University of Central Africa, Yaounde, Cameroon
Serge Damase Oyong Assiene

Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon
Pulcherie Ngo Lend

Central Laboratory, Laquintinie Hospital, Douala, Cameroon
Marthe Viviane Fonkam

School of Health Sciences, Catholic University of Central Africa, Yaounde, Cameroon
Edouard OrlineToukap

School of Health Sciences, Catholic University of Central Africa, Yaounde, Cameroon
Aurelle Yondo

College of Veterinary Medicine, The University of Georgia, Athens, Georgia
Lucien Honore Etame Sone

Department of Biology, Higher Institute of Medical Technology, Yaounde, Cameroon

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APA Style

Cedric Gueguim, Vanessa Kenny Megam, Serge Damase Oyong Assiene, Pulcherie Ngo Lend, Marthe Viviane Fonkam, et al. (2022). Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon. International Journal of Microbiology and Biotechnology, 7(4), 163-168. https://doi.org/10.11648/j.ijmb.20220704.12

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ACS Style

Cedric Gueguim; Vanessa Kenny Megam; Serge Damase Oyong Assiene; Pulcherie Ngo Lend; Marthe Viviane Fonkam, et al. Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon. Int. J. Microbiol. Biotechnol. 2022, 7(4), 163-168. doi: 10.11648/j.ijmb.20220704.12

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AMA Style

Cedric Gueguim, Vanessa Kenny Megam, Serge Damase Oyong Assiene, Pulcherie Ngo Lend, Marthe Viviane Fonkam, et al. Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon. Int J Microbiol Biotechnol. 2022;7(4):163-168. doi: 10.11648/j.ijmb.20220704.12

Copy | Download

@article{10.11648/j.ijmb.20220704.12,
  author = {Cedric Gueguim and Vanessa Kenny Megam and Serge Damase Oyong Assiene and Pulcherie Ngo Lend and Marthe Viviane Fonkam and Edouard OrlineToukap and Aurelle Yondo and Lucien Honore Etame Sone},
  title = {Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {7},
  number = {4},
  pages = {163-168},
  doi = {10.11648/j.ijmb.20220704.12},
  url = {https://doi.org/10.11648/j.ijmb.20220704.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220704.12},
  abstract = {Urinary tract infections, usually caused by bacteria that originate from the intestinal flora or the perineal flora, constitute a major public health problem for Africa in general, especially in Cameroon. Known failures with empiric therapy are becoming increasingly problematic. The same is proven true for the consistent bacterial resistance to antibiotics. The purpose of this study was to determine the frequency of Gram-negative bacterial strains isolated from urine samples at Laquintinie Hospital of Douala and to establish their antibiotic resistance profile. Over a period of three months, four hundred and thirty-six (436) urine samples were taken and analyzed at the bacteriology laboratory of the Laquintinie hospital in Douala. The Bacteria were isolated according to standard methods and identification using the API20E gallery. The antibiotic sensitivity profile of the isolated bacteria was done by the disk diffusion method according to the recommendations of the Antibiogram Committee of the French Society of Microbiology 2018. One hundred and fifty-one (151) bacterial strains were isolated, mostly from women (58.67%) with a male or female (M/F) ratio of 0.7. We noted a predominance of Escherichia coli strains (58.28%), followed by Klebsiella Pneumoniae (22.51%), Proteus mirabilis (4.64%), Pseudomonas aeruginosa (3.97%), Enterobacter cloacae (2.65%), Acinetobacter (1.98%), Enterobacter sakazakii (1.32%) and the rest 4.65%. All the bacterial strains isolated showed strong resistance to beta-lactam (greater than 50%) but were predominantly susceptible to Imipenem (96.39%) and Amikacin (90.47%). Escherichia coli was particularly resistant to beta-lactams such as amoxicillin (87.5%), amoxicillin + clavulanic acid (85.24%), Cefuroxime (65.57%), Cefixime (54.83%) and Ceftriaxone (52.70%). The bacterial strains of Klebsiella pneumoniae obtained a total resistance to amoxicillin (100%), clavulanic acid + amoxicillin (69.23%), Cefixime (68.42%), Ceftriaxone (52.70%), and Cefuroxime (52.00%). Escherichia coli (58.28%) and Klebsiella pneumoniae (22.51%) are the two main bacterial species that are the most isolated. Resistance of Escherichia coli and Klebsiella pneumoniae to antibiotics is a growing phenomenon. Monitoring for bacterial resistance to antibiotics is imperative in our context. Develop more effective prevention strategies, screening, prophylactic isolation.},
 year = {2022}
}

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TY  - JOUR
T1  - Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon
AU  - Cedric Gueguim
AU  - Vanessa Kenny Megam
AU  - Serge Damase Oyong Assiene
AU  - Pulcherie Ngo Lend
AU  - Marthe Viviane Fonkam
AU  - Edouard OrlineToukap
AU  - Aurelle Yondo
AU  - Lucien Honore Etame Sone
Y1  - 2022/10/21
PY  - 2022
N1  - https://doi.org/10.11648/j.ijmb.20220704.12
DO  - 10.11648/j.ijmb.20220704.12
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 163
EP  - 168
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20220704.12
AB  - Urinary tract infections, usually caused by bacteria that originate from the intestinal flora or the perineal flora, constitute a major public health problem for Africa in general, especially in Cameroon. Known failures with empiric therapy are becoming increasingly problematic. The same is proven true for the consistent bacterial resistance to antibiotics. The purpose of this study was to determine the frequency of Gram-negative bacterial strains isolated from urine samples at Laquintinie Hospital of Douala and to establish their antibiotic resistance profile. Over a period of three months, four hundred and thirty-six (436) urine samples were taken and analyzed at the bacteriology laboratory of the Laquintinie hospital in Douala. The Bacteria were isolated according to standard methods and identification using the API20E gallery. The antibiotic sensitivity profile of the isolated bacteria was done by the disk diffusion method according to the recommendations of the Antibiogram Committee of the French Society of Microbiology 2018. One hundred and fifty-one (151) bacterial strains were isolated, mostly from women (58.67%) with a male or female (M/F) ratio of 0.7. We noted a predominance of Escherichia coli strains (58.28%), followed by Klebsiella Pneumoniae (22.51%), Proteus mirabilis (4.64%), Pseudomonas aeruginosa (3.97%), Enterobacter cloacae (2.65%), Acinetobacter (1.98%), Enterobacter sakazakii (1.32%) and the rest 4.65%. All the bacterial strains isolated showed strong resistance to beta-lactam (greater than 50%) but were predominantly susceptible to Imipenem (96.39%) and Amikacin (90.47%). Escherichia coli was particularly resistant to beta-lactams such as amoxicillin (87.5%), amoxicillin + clavulanic acid (85.24%), Cefuroxime (65.57%), Cefixime (54.83%) and Ceftriaxone (52.70%). The bacterial strains of Klebsiella pneumoniae obtained a total resistance to amoxicillin (100%), clavulanic acid + amoxicillin (69.23%), Cefixime (68.42%), Ceftriaxone (52.70%), and Cefuroxime (52.00%). Escherichia coli (58.28%) and Klebsiella pneumoniae (22.51%) are the two main bacterial species that are the most isolated. Resistance of Escherichia coli and Klebsiella pneumoniae to antibiotics is a growing phenomenon. Monitoring for bacterial resistance to antibiotics is imperative in our context. Develop more effective prevention strategies, screening, prophylactic isolation.
VL  - 7
IS  - 4
ER  -

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