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Physicochemical and Bacteriological Study of Sewage Before and After Treatment with Moringa Oleifera Seeds at Brazzaville (Congo)

Received: 3 March 2021    Accepted: 25 March 2021    Published: 13 April 2021
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Abstract

Within the framework of the mobilization of water resources and the supply of drinking water to the populations as well as the valuation of local materials, four (4) samples of piped water from the districts of Brazzaville were analyzed for their physical quality. chemical and microbiological before and after treatment with Moringa oleifera seed powder. The physicochemical parameters were determined using standard devices. As for the microbiological parameters, they were analyzed by conventional microbiological methods which consisted of isolation on selective media, identification using cultural, morphological and biochemical criteria and then counting. The results obtained concerning the physicochemical parameters showed that the raw water exhibited pH values ranging from 6.4 to 7.02; temperatures ranging from 25 to 25.8 with high values of turbidity and dry matter. However, low levels of magnesium and calcium ions have been noted. Regarding the microbiological parameters, this water exhibited bacteria of the genus Staphylococcus as well as fecal and total coliforms, a large number of which were unidentified. The genera Salmonella and Shigella have been identified as well as the species E. coli, an indicator of contamination. After clarification of the Moringa beans with powder, the pH values varied from 5.40 to 4.02; there is a decrease in turbidity as well as the dry matter. Microbiologically, the clarified water contained unidentified coliforms with persistence of Salmonella and Staphylococci in low proportions. As for E coli and Shigella, they have been totally eliminated. After clarification and decantation, the results show that all the microorganisms are found concentrated in the sludge. Before treatment, the cloudy pipe water exhibited high concentrations of organic and mineral chemicals. These analyzed waters contained several types of germs in very high numbers. The muddy water is decanted, clarified and purified by the powder of Moringa oleifera seeds in less than 2 hours. However, there is a decrease in the number of germs in the clarified water with the formation of an activated sludge. Moringa oléifera is therefore a local plant material that can be used in the treatment of wastewater to prevent possible contamination.

Published in International Journal of Microbiology and Biotechnology (Volume 6, Issue 2)
DOI 10.11648/j.ijmb.20210602.11
Page(s) 28-33
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Muddy Water, Pipeline, Quality, Treatment, Moringa Oléifera

References
[1] Barhoumi-Andréani Y. (2004). Eau: Ressources et Menaces, Water: Resources and Threats 47p. https://www.etudier.com/dissertations/Eau-Ressources-Et-Menaces/46418124.html.
[2] Pene P. et Levi Y. (2011). Les eaux de consommation humaine et la santé publique en France métropolitaine. Bulletin de l'Académie Nationale de Médecine. 195, 403-429.
[3] Gauthier F. (2002). Biofilms et qualité biologique de l’eau potable au cours de sa distribution; Mémoire de DESS de l’Université de Picardie Amiens, 78p.
[4] AFNOR, (1997). Water quality. Compilation of French Environmental Standards. Volumes 1, 2, 3 and 4. 1372 p.
[5] Manuel pratique d’analyse de l’eau, 2013, Fondation Nationale de la Santé Bresilia 4e Edition, P 21-24/153p. http://www.funasa.gov.br/site/wp.
[6] Makhoukh M., M. Sba1, A. Berrahou, M. Van. Clooster. (2011). Contribution to the physico-chemical study of the surface waters of the moulouyawadi (Eastern Morocco) Larhyss Journal, ISSN 1112-3680, n° 09: 150-169.
[7] Abouelouafa Mounia, El Halouani Hassan, Kharboua Mohammed & Berrichi Abdel bass. (2002). Physical, chemical and bacteriological characterization of raw wastewater in the city of Oujda: main canal and Oued Bounaïm. Inst. Acts Agron. Vet. (Morocco). Vol. 22, (3) 143-150.
[8] Guamri Y. El, D. Belghyti, M. Cisse, K. El Kharrim, I. Sylla, S. Raweh, H. Barkia, T. Hassouni and A. Jamber. (2007). Physico-chemical and parasitological study of wastewater intended for irrigation of the peri-urban perimeter of Fouarat (Kenitra, Morocco). African Agronomy. 19 (3): 251-261.
[9] Belghyti Driss, Youssef El Guamri, Ghizlane Ztit, My. Lahcen Ouahidi, My Brahim Joti, Abdelatif Harchrass et al (2009). Physico-chemical characterisation of slaughterhouse waste water with a view to implementing appropriate treatment: case of Kenitra in Morocco. Africa science. 05 (2) 153-216.
[10] Nola M., T. Njiné, N. Kemka, S. H. Zébazé Togouet, P. Servais, M. Messouli et al. (2006). Transfer of faecal bacteria to a water table through a soil column in the equatorial region: Influence of the water load applied to the surface. Revue des sciences de l'eau. vol. 19, no 2. 101-112.
[11] Baloki Ngoulou Tarcisse, Moyen Rachel, Nguimbi Etienne, Ahombo Gabriel, Matini Laurent. (2019). Study of the Physico-Chemical and Microbiological Parameters of Household Wastewater in Brazzaville. International Journal of Environmental Science. ISSN: 2367-8941; Vol 4: 80 – 90. http://www.iaras.org/iaras/journals/ijes.
[12] Pkia Eddabra. (2011). Evaluation of bacteriological contamination of wastewater from wastewater treatment plants of the large AGADIR: Isolation, molecular characterization and antibiotic resistance of vibrio species. Thesis. 1-146.
[13] Parihar S. S., Kumar Ajit, Kumar Ajay, Gupta R. N., Pathak Manoj, Shrivastav Archana and Pandey A. C. (2012). Physico-Chemical and Microbiological Analysis of Underground Water in and Around Gwalior City, MP, India Research Journal of Recent Sciences. ISSN 2277-2502. Vol. 1 (6): 62-65.
[14] Mukhopadhyay Sabyasachi, Ratul Mukherjee. (2013). Physico - Chemical and microbiological quality assessment of groundwater in adjoining area of Tamla Nala, Durgapur, District: Burdwan (W. B.), India. Environmental sciences issn 0976 - 4402 Volume 4, No 3: 360-366.
[15] Ouali El Lalami A., Merzouki M., El Hillali O., Maniar S., IbnsoudaKoraichi S. (2010). Pollution of surface water in the City of FES in Morocco: typology, origin and consequences. Larhyss journal, ISSN 1112-3680, n° 09: 55-72.
[16] Amghar H. (2002). Assessment of the impact of metal pollution on the waters of Lake Fouarat (Gharb region). Postgraduate thesis, Fac. Sci., Kenitra, 1-113. Available at: www.ebanque-pdf.com/fr_water-metallic-pollution.html.
[17] Srivastava Kumar Ratna and Deepti Pandey. (2013). Physico -chemical and microbiological quality evaluation of groundwater for human domestic consumption in adjoining area of OmtiNallah, Jabalpur (M. P.), India Environmental Sciences ISSN 0976 - 4402. Vol 3, No 4: 1300-1307.
[18] Attab Sarah. (2011). Improvement of the microbiological quality of the water treated with activated sludge from the Haoud Berkaoui wastewater treatment plant by using a local sand filter. Magister in Algeria. 1-152p.
[19] Niang Sophie. (1998). Purification of urban wastewater by reconstituted hydrosers. Experimental research on a technology appropriate for West Africa. Reports which. Were presented for the Cities Feeding People workshop: "Lessons learned from urban agriculture projects in African cities" Nairobi, Kenya, 1-67.
[20] Fathallah Z., Elkharrim K., Fathallah R., Hbaiz E M., Hamid C., Ayyach A., Elkhadmaoui A., Belghyti D. (2014). Physico-chemical study of waste water from the paper industry unit (cdm) at Sidi Yahia El Gharb (Morocco).
[21] Ayress. R. M. 1997, Analyse des eaux résiduaires en vue de leur recyclage en agriculture. Manuel de techniques de laboratoire en parasitologie et bactériologie Genève: OMS, P 30.
[22] Yao Akpo, 2006, Evaluation de la pollution des eaux usées domestiques collectées et traitées à la station d’épuration de Camberène (Dakar), Mémoire de D. E. A, Université Cheick Anta Diop, P 31.
[23] Aboulkacem A., A. Chahlaoui, A. Soulaymani, F. Rhazi-Filaliet D. Benali. (2007). Bacteriological comparative study of the waters of the Boufekrane and Ouislanewadis crossing the city of Meknes (Morocco). Discount, Vol. 1, No. 1. 10-22.
[24] Folkard. G, J. Sutherland et R. Shaw. R, (1999). Water Clarification using Moringa oleifera. Technical Information W1e. P. 1-7.
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    Moyen Rachel, Serge Mdjouetede, Nguimbi Etienne, Baloki Ngoulou Tarcisse, Matini Laurent. (2021). Physicochemical and Bacteriological Study of Sewage Before and After Treatment with Moringa Oleifera Seeds at Brazzaville (Congo). International Journal of Microbiology and Biotechnology, 6(2), 28-33. https://doi.org/10.11648/j.ijmb.20210602.11

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    ACS Style

    Moyen Rachel; Serge Mdjouetede; Nguimbi Etienne; Baloki Ngoulou Tarcisse; Matini Laurent. Physicochemical and Bacteriological Study of Sewage Before and After Treatment with Moringa Oleifera Seeds at Brazzaville (Congo). Int. J. Microbiol. Biotechnol. 2021, 6(2), 28-33. doi: 10.11648/j.ijmb.20210602.11

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    AMA Style

    Moyen Rachel, Serge Mdjouetede, Nguimbi Etienne, Baloki Ngoulou Tarcisse, Matini Laurent. Physicochemical and Bacteriological Study of Sewage Before and After Treatment with Moringa Oleifera Seeds at Brazzaville (Congo). Int J Microbiol Biotechnol. 2021;6(2):28-33. doi: 10.11648/j.ijmb.20210602.11

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  • @article{10.11648/j.ijmb.20210602.11,
      author = {Moyen Rachel and Serge Mdjouetede and Nguimbi Etienne and Baloki Ngoulou Tarcisse and Matini Laurent},
      title = {Physicochemical and Bacteriological Study of Sewage Before and After Treatment with Moringa Oleifera Seeds at Brazzaville (Congo)},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {6},
      number = {2},
      pages = {28-33},
      doi = {10.11648/j.ijmb.20210602.11},
      url = {https://doi.org/10.11648/j.ijmb.20210602.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20210602.11},
      abstract = {Within the framework of the mobilization of water resources and the supply of drinking water to the populations as well as the valuation of local materials, four (4) samples of piped water from the districts of Brazzaville were analyzed for their physical quality. chemical and microbiological before and after treatment with Moringa oleifera seed powder. The physicochemical parameters were determined using standard devices. As for the microbiological parameters, they were analyzed by conventional microbiological methods which consisted of isolation on selective media, identification using cultural, morphological and biochemical criteria and then counting. The results obtained concerning the physicochemical parameters showed that the raw water exhibited pH values ranging from 6.4 to 7.02; temperatures ranging from 25 to 25.8 with high values of turbidity and dry matter. However, low levels of magnesium and calcium ions have been noted. Regarding the microbiological parameters, this water exhibited bacteria of the genus Staphylococcus as well as fecal and total coliforms, a large number of which were unidentified. The genera Salmonella and Shigella have been identified as well as the species E. coli, an indicator of contamination. After clarification of the Moringa beans with powder, the pH values varied from 5.40 to 4.02; there is a decrease in turbidity as well as the dry matter. Microbiologically, the clarified water contained unidentified coliforms with persistence of Salmonella and Staphylococci in low proportions. As for E coli and Shigella, they have been totally eliminated. After clarification and decantation, the results show that all the microorganisms are found concentrated in the sludge. Before treatment, the cloudy pipe water exhibited high concentrations of organic and mineral chemicals. These analyzed waters contained several types of germs in very high numbers. The muddy water is decanted, clarified and purified by the powder of Moringa oleifera seeds in less than 2 hours. However, there is a decrease in the number of germs in the clarified water with the formation of an activated sludge. Moringa oléifera is therefore a local plant material that can be used in the treatment of wastewater to prevent possible contamination.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Physicochemical and Bacteriological Study of Sewage Before and After Treatment with Moringa Oleifera Seeds at Brazzaville (Congo)
    AU  - Moyen Rachel
    AU  - Serge Mdjouetede
    AU  - Nguimbi Etienne
    AU  - Baloki Ngoulou Tarcisse
    AU  - Matini Laurent
    Y1  - 2021/04/13
    PY  - 2021
    N1  - https://doi.org/10.11648/j.ijmb.20210602.11
    DO  - 10.11648/j.ijmb.20210602.11
    T2  - International Journal of Microbiology and Biotechnology
    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
    SP  - 28
    EP  - 33
    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20210602.11
    AB  - Within the framework of the mobilization of water resources and the supply of drinking water to the populations as well as the valuation of local materials, four (4) samples of piped water from the districts of Brazzaville were analyzed for their physical quality. chemical and microbiological before and after treatment with Moringa oleifera seed powder. The physicochemical parameters were determined using standard devices. As for the microbiological parameters, they were analyzed by conventional microbiological methods which consisted of isolation on selective media, identification using cultural, morphological and biochemical criteria and then counting. The results obtained concerning the physicochemical parameters showed that the raw water exhibited pH values ranging from 6.4 to 7.02; temperatures ranging from 25 to 25.8 with high values of turbidity and dry matter. However, low levels of magnesium and calcium ions have been noted. Regarding the microbiological parameters, this water exhibited bacteria of the genus Staphylococcus as well as fecal and total coliforms, a large number of which were unidentified. The genera Salmonella and Shigella have been identified as well as the species E. coli, an indicator of contamination. After clarification of the Moringa beans with powder, the pH values varied from 5.40 to 4.02; there is a decrease in turbidity as well as the dry matter. Microbiologically, the clarified water contained unidentified coliforms with persistence of Salmonella and Staphylococci in low proportions. As for E coli and Shigella, they have been totally eliminated. After clarification and decantation, the results show that all the microorganisms are found concentrated in the sludge. Before treatment, the cloudy pipe water exhibited high concentrations of organic and mineral chemicals. These analyzed waters contained several types of germs in very high numbers. The muddy water is decanted, clarified and purified by the powder of Moringa oleifera seeds in less than 2 hours. However, there is a decrease in the number of germs in the clarified water with the formation of an activated sludge. Moringa oléifera is therefore a local plant material that can be used in the treatment of wastewater to prevent possible contamination.
    VL  - 6
    IS  - 2
    ER  - 

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Author Information
  • Laboratory of Cellular and Molecular Biology, Sciences and Techniques Faculty, Marien Ngouabi University, Brazzaville, Congo

  • Laboratory of Cellular and Molecular Biology, Sciences and Techniques Faculty, Marien Ngouabi University, Brazzaville, Congo

  • Laboratory of Cellular and Molecular Biology, Sciences and Techniques Faculty, Marien Ngouabi University, Brazzaville, Congo

  • Laboratory of Cellular and Molecular Biology, Sciences and Techniques Faculty, Marien Ngouabi University, Brazzaville, Congo

  • Laboratory of Applied Mineral Chemistry, Sciences and Techniques Faculty, Marien Ngouabi University, Brazzaville, Congo

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