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Screening and Identification of Flocculant Producing Bacteria and Its Application in Wastewater

Received: 4 March 2021    Accepted: 17 March 2021    Published: 26 March 2021
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Abstract

Background: with the increasingly serious global water pollution, industrial and agricultural wastewater treatment is becoming more and more important. Flocculant precipitation is the first process in wastewater treatment. One of the keys to achieve efficient water purification is to add microbial flocculant with excellent performance. Objective: the research of high-efficiency and non-toxic microbial flocculant for wastewater treatment has increasingly become important in environmental protection. Method: CMC-Na medium and dilute iodine staining was used to separate and screen flocculant producing bacteria. The distribution of flocculant activity and the effect of different types of wastewater treatment were studied. Results: the flocculant producing strain 71-1 separate from ruminant feces was identified as Cellulosimicrobiums sp. The flocculating rate of MBF-P71 was 97.83%, which had obvious degradation effect on COD value of pig farm manure water and ship port sewage. The removal rates of cod were 66.95% and 46.68% respectively. Conclusion: MBF-P71 has the advantages of good flocculation effect, low production cost, high efficiency and low dosage. It has a good development and application prospect for wastewater treatment.

Published in International Journal of Microbiology and Biotechnology (Volume 6, Issue 1)
DOI 10.11648/j.ijmb.20210601.13
Page(s) 21-27
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Microbial Flocculant, Cellulosimicrobiums, Screening and Identification, Sewage Purification, Flocculant-producing Strain, Wastewater

References
[1] Suresh A, Grygolowicz-Pawlak E, Pathak S, et al. (2018) Understanding and optimization of the flocculation process in biological wastewater treatment processes: A review. Chemosphere. 210: 401-416.
[2] Zhao C, Yang Q, Zhang H, et al. (2017) Optimization of Microbial Flocculant-Producing Medium for Bacillus subtilis. Indian J Microbiol. 57: 83-91.
[3] Michel Doumith, Carmen Buchrieser, Philippe Glaser, et al.(2004) Differentiation of the Major Listeria monocytogenes Serovars by Multiplex PCR. J. Clin. Microbiol., 42: 3819–3822.
[4] ZHANG Xiang-mei, MA Tong-suo, ZHANG Hong-bing. (2006) Screening of a new flocculant producing strain and its characteristics. Industrial Water & Wastewater, 37: 64-66.
[5] Fang Zhenhua, Zeng Yumei, Hong Meiling, et al. (2019) Isolation, identification and drug sensitivity test of pathogenic Aeromonas Vickers from Chinese striped neck turtle. Chin. Animal Husbandry & Vet. Medicine, 46: 2760-2768.
[6] Kurane R, Toeda K, Suzuki T. (1986) Culture conditions for production of microbial flocculant by Rhodococcus erythropolis. Agric. Biol. Chem. 50: 2309-2313.
[7] Suh H H, Kwon G S, Lee C H, et al. (1997) Characterization of bioflocculant produced by Bacillus sp. DP-152. Journal of Fermentation And Bioengineering 84: 108-112.
[8] SchumannP, Weiss N, Stackebrandt E. (2001) Reclassification of Cellulomonas cellulans (Stackebrandt and Keddie 1986) as Cellulosimicrobium cellulans gen. nov., comb. Nov. Int J Syst Evol Microbiol, 51: 1007-1010.
[9] Agunbiade MO, Van Heerden E, Pohl CH, et al. (2017) Flocculating performance of a bioflocculant produced by Arthrobacter humicola in sewage waste water treatment. BMC Biotechnol. 12; 17: 51.
[10] Mu J, Cui X, Shao M, Wang Y, Yang Q, Yang G, et al. (2019) Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China. PLoS One. 19; 14: e0217679.
[11] Zhu Dan, Yang Lin, Su Hongyan. (2010) Isolation of Flocculant-producing Bacteria from Erhai Lake Sediment and Optimization of Culture Conditions. Journal of Dali university 09: 43-45.
[12] Ma Yiwen, Wang Mingming (2014) Study on flocculation of high-flocculation bacteria in potato starch wastewater. Food Industry Technology, 35: 170-173.
[13] Yang Jinfeng, Zhao Jihong, Song Feng-Hua (2009) Study on Decolorization of Dye Wastewater by Microbial Flocculant and Its Kinetics. Science & Technology in Chemical Industry, 17: 16-19.
[14] Wang T, Tang X, Zhang S, et al. (2020) Roles of functional microbial flocculant in dyeing wastewater treatment: Bridging and adsorption. J Hazard Mater. 15; 384: 121506.
[15] Xia X, Lan S, Li X, et al. (2018) Characterization and coagulation-flocculation performance of a composite flocculant in high-turbidity drinking water treatment. Chemosphere. 206: 701-08.
[16] Ma Fang, Yang jixian. (2013) Compound Microbial Flocculant. Beijing: Science Press.
Cite This Article
  • APA Style

    Sha Sha Zhang, Ming Li, Li Fang Wang, Xiu Dong Wang, Jian Feng Xu. (2021). Screening and Identification of Flocculant Producing Bacteria and Its Application in Wastewater. International Journal of Microbiology and Biotechnology, 6(1), 21-27. https://doi.org/10.11648/j.ijmb.20210601.13

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    ACS Style

    Sha Sha Zhang; Ming Li; Li Fang Wang; Xiu Dong Wang; Jian Feng Xu. Screening and Identification of Flocculant Producing Bacteria and Its Application in Wastewater. Int. J. Microbiol. Biotechnol. 2021, 6(1), 21-27. doi: 10.11648/j.ijmb.20210601.13

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    AMA Style

    Sha Sha Zhang, Ming Li, Li Fang Wang, Xiu Dong Wang, Jian Feng Xu. Screening and Identification of Flocculant Producing Bacteria and Its Application in Wastewater. Int J Microbiol Biotechnol. 2021;6(1):21-27. doi: 10.11648/j.ijmb.20210601.13

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  • @article{10.11648/j.ijmb.20210601.13,
      author = {Sha Sha Zhang and Ming Li and Li Fang Wang and Xiu Dong Wang and Jian Feng Xu},
      title = {Screening and Identification of Flocculant Producing Bacteria and Its Application in Wastewater},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {6},
      number = {1},
      pages = {21-27},
      doi = {10.11648/j.ijmb.20210601.13},
      url = {https://doi.org/10.11648/j.ijmb.20210601.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20210601.13},
      abstract = {Background: with the increasingly serious global water pollution, industrial and agricultural wastewater treatment is becoming more and more important. Flocculant precipitation is the first process in wastewater treatment. One of the keys to achieve efficient water purification is to add microbial flocculant with excellent performance. Objective: the research of high-efficiency and non-toxic microbial flocculant for wastewater treatment has increasingly become important in environmental protection. Method: CMC-Na medium and dilute iodine staining was used to separate and screen flocculant producing bacteria. The distribution of flocculant activity and the effect of different types of wastewater treatment were studied. Results: the flocculant producing strain 71-1 separate from ruminant feces was identified as Cellulosimicrobiums sp. The flocculating rate of MBF-P71 was 97.83%, which had obvious degradation effect on COD value of pig farm manure water and ship port sewage. The removal rates of cod were 66.95% and 46.68% respectively. Conclusion: MBF-P71 has the advantages of good flocculation effect, low production cost, high efficiency and low dosage. It has a good development and application prospect for wastewater treatment.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Screening and Identification of Flocculant Producing Bacteria and Its Application in Wastewater
    AU  - Sha Sha Zhang
    AU  - Ming Li
    AU  - Li Fang Wang
    AU  - Xiu Dong Wang
    AU  - Jian Feng Xu
    Y1  - 2021/03/26
    PY  - 2021
    N1  - https://doi.org/10.11648/j.ijmb.20210601.13
    DO  - 10.11648/j.ijmb.20210601.13
    T2  - International Journal of Microbiology and Biotechnology
    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
    SP  - 21
    EP  - 27
    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20210601.13
    AB  - Background: with the increasingly serious global water pollution, industrial and agricultural wastewater treatment is becoming more and more important. Flocculant precipitation is the first process in wastewater treatment. One of the keys to achieve efficient water purification is to add microbial flocculant with excellent performance. Objective: the research of high-efficiency and non-toxic microbial flocculant for wastewater treatment has increasingly become important in environmental protection. Method: CMC-Na medium and dilute iodine staining was used to separate and screen flocculant producing bacteria. The distribution of flocculant activity and the effect of different types of wastewater treatment were studied. Results: the flocculant producing strain 71-1 separate from ruminant feces was identified as Cellulosimicrobiums sp. The flocculating rate of MBF-P71 was 97.83%, which had obvious degradation effect on COD value of pig farm manure water and ship port sewage. The removal rates of cod were 66.95% and 46.68% respectively. Conclusion: MBF-P71 has the advantages of good flocculation effect, low production cost, high efficiency and low dosage. It has a good development and application prospect for wastewater treatment.
    VL  - 6
    IS  - 1
    ER  - 

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Author Information
  • Panjin Center for Inspection and Testing/Panjin CDC, Panjin, China

  • Heishan County Market Supervision Service Center, Jinzhou, China

  • Heishan County Market Supervision Service Center, Jinzhou, China

  • Panjin Center for Inspection and Testing/Panjin CDC, Panjin, China

  • Panjin Center for Inspection and Testing/Panjin CDC, Panjin, China

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