Background: Defective ERK1 activity may regulate the DNA hypomethylation in T cells of lupus mice. Aims: To investigate the function of ERK1 in regulating DNA hypomethylation and explore the potential molecular mechanisms involved in lupus mice. Methods: CD4+T cells were isolated from MRL/lpr and BALB/c mice, activated in vitro in the presence or absence of 5-azacytidine (5-Aza) (a DNA methyltransferase inhibitor)/U0126 (a selective inhibitor of the ERK signaling pathway) respectively. The positive rate of CD4+T cell was measured by flow cytometry, and expression levels of CD70, ERK1, DNA transferase 1 (DNMT1) were estimated by RT-PCR and Western blot. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. Results: The expression level of p-ERK1 in MRL/lpr mice was significantly lower than healthy control (P<0.05); Meanwhile, down regulation of DNMT1 and DNA hypomethylation were found in MRL/lpr mice T cells. In contrary, the expression levels of CD70 increased when compared with healthy control (P<0.05). The same changes were seen in healthy CD4+T cells treated with U0126. Conclusions: DNA hypomethylation regulating mechanisms in lupus T cells were associated with ERK1 signal pathway. U0126 could inhibit ERK1 signal transduction pathway in normal T cells, and induce DNA hypomethylation by regulating the expression of methylation sensitive gene CD70.
| Published in | International Journal of Immunology (Volume 9, Issue 3) |
| DOI | 10.11648/j.iji.20210903.11 |
| Page(s) | 41-46 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Systemic Lupus Erythematosus, DNA Methylation, ERK1 Signal Pathway, Epigenetics
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APA Style
Miaoxuan Luo, Shanshan Wei, Xiangbin Mi, Tangde Zhang, Wu Zhang. (2021). The Regulatory Effect of ERK1 Pathway in the DNA Hypomethylation of MRL/lpr Mice. International Journal of Immunology, 9(3), 41-46. https://doi.org/10.11648/j.iji.20210903.11
ACS Style
Miaoxuan Luo; Shanshan Wei; Xiangbin Mi; Tangde Zhang; Wu Zhang. The Regulatory Effect of ERK1 Pathway in the DNA Hypomethylation of MRL/lpr Mice. Int. J. Immunol. 2021, 9(3), 41-46. doi: 10.11648/j.iji.20210903.11
AMA Style
Miaoxuan Luo, Shanshan Wei, Xiangbin Mi, Tangde Zhang, Wu Zhang. The Regulatory Effect of ERK1 Pathway in the DNA Hypomethylation of MRL/lpr Mice. Int J Immunol. 2021;9(3):41-46. doi: 10.11648/j.iji.20210903.11
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Download@article{10.11648/j.iji.20210903.11,
author = {Miaoxuan Luo and Shanshan Wei and Xiangbin Mi and Tangde Zhang and Wu Zhang},
title = {The Regulatory Effect of ERK1 Pathway in the DNA Hypomethylation of MRL/lpr Mice},
journal = {International Journal of Immunology},
volume = {9},
number = {3},
pages = {41-46},
doi = {10.11648/j.iji.20210903.11},
url = {https://doi.org/10.11648/j.iji.20210903.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20210903.11},
abstract = {Background: Defective ERK1 activity may regulate the DNA hypomethylation in T cells of lupus mice. Aims: To investigate the function of ERK1 in regulating DNA hypomethylation and explore the potential molecular mechanisms involved in lupus mice. Methods: CD4+T cells were isolated from MRL/lpr and BALB/c mice, activated in vitro in the presence or absence of 5-azacytidine (5-Aza) (a DNA methyltransferase inhibitor)/U0126 (a selective inhibitor of the ERK signaling pathway) respectively. The positive rate of CD4+T cell was measured by flow cytometry, and expression levels of CD70, ERK1, DNA transferase 1 (DNMT1) were estimated by RT-PCR and Western blot. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. Results: The expression level of p-ERK1 in MRL/lpr mice was significantly lower than healthy control (PP+T cells treated with U0126. Conclusions: DNA hypomethylation regulating mechanisms in lupus T cells were associated with ERK1 signal pathway. U0126 could inhibit ERK1 signal transduction pathway in normal T cells, and induce DNA hypomethylation by regulating the expression of methylation sensitive gene CD70.},
year = {2021}
}
TY - JOUR T1 - The Regulatory Effect of ERK1 Pathway in the DNA Hypomethylation of MRL/lpr Mice AU - Miaoxuan Luo AU - Shanshan Wei AU - Xiangbin Mi AU - Tangde Zhang AU - Wu Zhang Y1 - 2021/08/18 PY - 2021 N1 - https://doi.org/10.11648/j.iji.20210903.11 DO - 10.11648/j.iji.20210903.11 T2 - International Journal of Immunology JF - International Journal of Immunology JO - International Journal of Immunology SP - 41 EP - 46 PB - Science Publishing Group SN - 2329-1753 UR - https://doi.org/10.11648/j.iji.20210903.11 AB - Background: Defective ERK1 activity may regulate the DNA hypomethylation in T cells of lupus mice. Aims: To investigate the function of ERK1 in regulating DNA hypomethylation and explore the potential molecular mechanisms involved in lupus mice. Methods: CD4+T cells were isolated from MRL/lpr and BALB/c mice, activated in vitro in the presence or absence of 5-azacytidine (5-Aza) (a DNA methyltransferase inhibitor)/U0126 (a selective inhibitor of the ERK signaling pathway) respectively. The positive rate of CD4+T cell was measured by flow cytometry, and expression levels of CD70, ERK1, DNA transferase 1 (DNMT1) were estimated by RT-PCR and Western blot. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. Results: The expression level of p-ERK1 in MRL/lpr mice was significantly lower than healthy control (PP+T cells treated with U0126. Conclusions: DNA hypomethylation regulating mechanisms in lupus T cells were associated with ERK1 signal pathway. U0126 could inhibit ERK1 signal transduction pathway in normal T cells, and induce DNA hypomethylation by regulating the expression of methylation sensitive gene CD70. VL - 9 IS - 3 ER -
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