Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats

Safaa Ahmed Salem; Amany Mohammed Gad; Abdellatif Anaam Kamal

doi:doi:10.11648/j.ijfsb.20210602.11

| Peer-Reviewed

Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats

Safaa Ahmed Salem, Amany Mohammed Gad, Abdellatif Anaam Kamal

Published in International Journal of Food Science and Biotechnology (Volume 6, Issue 2)

Received: 2 March 2021 Accepted: 24 March 2021 Published: 7 April 2021

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

In this study beetroot and eggplant were used as natural source for production of anti-anemic functional food for anemic patients and to prevent it during disease or chemotherapy treatment. Anemic model was induced in albino female rats by daily injection with phenylhydrazin (5mg/kg b.w) for three successive days, then biochemical, hematological and histological parameters were determined including; iron, ferritin, Ascorbic acid, Hepicidin, Hemoglobin and Complete blood count (men corpuscle volume, men corpuscle hemoglobin, haematocriet, red blood cells and white blood cells). Negative control group of normal rats, and positive control group of anemic rats each of six rats were fed on basal diet. Also 24 anemic rats were divided into 4 groups each of 6 rats which were fed on basal diet containing either eggplant or beetroot in ratios of 100, 300g/kg, in addition to 500mg ascorbic acid and 2mg folic acid/ kg diet for all groups for 30 days. feeding period was 30 days. At the end of experiment the above biochemical and haematological parameters were evaluated and the liver and spleen were histopathological examined. Decreases in hemoglobin, men corpuscle volume, haematocriet, red blood cells, men corpuscle hemoglobin, iron, ascorbic acid and ferritin, also increases in white blood cells and hepcidin were observed in anemic model rats. At the end of feeding period with the previous supplemented tested basal diet, increases in all aforementioned parameters, except white blood cells and hepcidin which showed decreases compared to anemic positive group were found.

Published in	International Journal of Food Science and Biotechnology (Volume 6, Issue 2)
DOI	10.11648/j.ijfsb.20210602.11
Page(s)	21-29
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Anemic, Beetroot, Chemical Properties, Eggplant, Hematological, Histopathologjcal

References

[1]	Chauhan, SP, Sheth, NR, Suhagia, BN (2015). Hematinic effect of fruits of Opuntia elatior Mill. on phenylhydrazine-induced anemia in rats,” AYU (An Int. Q. J. Res. Ayurveda. 36 (2): 208-213.
[2]	Ali, B, Al Za'abi M, Ramkumar A, Yasin J, Nemmar, A (2014). Anemia in adenine-induced chronic renal failure and the influence of treatment with gum acacia thereon. Physiol. Res. 63 (3): 351–358.
[3]	Petek, ET, Sergio, A G, Erand, L, Peter, Francis, R, Lyda, Z R, Beatrice, MW, Doris, KH, Wolf, EH, Michele, F E, Oscar, HF, Marija, G, Taulant, M. (2020). Anemia and iron metabolism in COVID- 19: A systematic review and meta- analysis. 35 (8): 763-773.
[4]	Zheying, T, Mingyu, L, Jingyi, W, Jing, X, Wei, C, Zhitao, Y, Ling, L, Ruwu, C, Jingyuan, X, Huiming, W, Jialin, L.(2019) “Anaemia is Associated with Severe Illness in COVID-19: A Retrospective Cohort Study. Res. Sq.
[5]	Ruedin, P, Dickenmann, M, Martin, P, Wüthrich, R. (2012). Management of renal anemia in patients with chronic kidney disease: the role of the general practitioner. Rev. Med. Suisse. 8 (323): 70–73.
[6]	Pantopoulos, K, Porwal, S K, Tartakoff, A, Devireddy, L. (2012). Mechanisms of mammalian iron homeostasis. Biochem. 51:. 5705–5724.
[7]	Ogbe, R, Adoga, G, Abu, A. (2010). Antianaemic potentials of some plant extracts on phenyl hydrazine-induced anaemia in rabbits. J. Med. Plants Res. 4 (8): 680–684.
[8]	Berger, J. (2007). Phenylhydrazine haematotoxicity. J. Appl. Biomed. 5 (3): 125–130.
[9]	Rubatzky, VE, Yamaguchi, M. (2012). World vegetables: principles, production, and nutritive values Springer Sci. Bus. Media.
[10]	Kowalska, G., Wiercinski, J. (2003). Chemical composition of fruits of three eggplant (Solanum melongena L.) cultivars. Folia Hortic. (Poland). 15 (2) 89–95.
[11]	Siervo, M, Lara, J, Ogbonmwan, I., Mathers, JC. (2013). Inorganic Nitrate and Beetroot Juice Supplementation Reduces Blood Pressure in Adults: A Systematic Review and Meta-Analysis. J. Nutr. 143 (6): 818–826.
[12]	J. M. Čanadanović-Brunet et al.(2011) “Antioxidant and antimicrobial activities of beet root pomace extracts. Czech J. Food Sci. 29 (6): 575–585.
[13]	Sahore K, Rani S. (2019). A review on medicinal importance, pharmacological activity and toxicology ofNutraceuticals. 8 (2): 441–449.
[14]	A. O. A. C, “fficial methods of analysis of AOAC international 20^th (ed) current through revision 2. Atkinson,, M. A. & furth, S. L. 2011.
[15]	Szterk A, Roszko M, Małek K, Czerwonka M, Waszkiewicz-Robak B. (2012). Application of the SPE reversed phase HPLC/MS technique to determine vitamin B12 bio-active forms in beef. Meat Sci. 91: 408–413.
[16]	Shermer G, Jones KC. (1967). Public Housing is the Tenants,” Washington, DC Natl. Assoc. Hous. Redev. Off.
[17]	Musyoka T, Dorothy N, Wycliffe A, Juma K, Nzioka M. (2016). In Vivo Antianaemic Effect and Safety of Aqueous Extracts of Erythrina abyssinica and Zanthoxylum usambarensis in Mice Models. J. Hemato Thromboembolic Dis. 4 (3): 1–10.
[18]	Drabkin, D L, Austin, J. H. (1935). Spectrophotometric studies II. Preparations from washed blood cells; nitric oxide hemoglobin and sulfhemoglobin. J Biol. Chem. 112 (l): 51–65.
[19]	Bancroft J, Gamble M. (2008). Theory and practice of histology techniques. Churchill Livingstone Elsevier. 83-134.
[20]	Kale, R, Sawate, A, Kshirsagar, R, Patil, B., Mane, R. (2018). Studies on evaluation of physical and chemical composition of beetroot (Beta vulgaris L.),” International journal of chemical studies. 6 (2): 2977–2979.
[21]	San José R, Plazas M, Sánchez-Mata MC, Cámara M, Prohens J. (2016). Diversity in composition of scarlet (S. aethiopicum) and gboma (S. macrocarpon) eggplants and of interspecific hybrids between S. aethiopicum and common eggplant (S. melongena), J. Food Compos. Anal. 45: 130–140.
[22]	Petek, M, Toth, N, Pecina, M., Karažija, T, Lazarević, B, Palčić, I, Veres, S., Ćustić, M H. (2019). Beetroot mineral composition affected by mineral and organic fertilization. PLoS One. 6: 1–15.
[23]	Arivalagan M., Gangopadhyay K., Kuma, G., Bhardwa, R., Prasad T., Sarkar S. K., Roy (2012). Variability in mineral composition of Indian eggplant (Solanum melongena L.) genotypes,” Journal of Food Composition and Analysis. 26 (1–2): 173–176.
[24]	Wolk, R, Shamsuzzaman, A S, Somers, VK. (2003). Obesity, sleep apnea, and hypertension. Hypertension. 42: 1067–1074.
[25]	Abbaspour N, Hurrell R, Kelishadi R (2014). Review on iron and its importance for human health. J. Res. Med. Sci. 19 (2): 164–174.
[26]	Khanum F, Anand T, Ilaiyaraja N, et al. (2017). Health Food for Soldiers. Def. Life Sci. Journal. 2 (2): 111–119.
[27]	Elaby SM, Ali JB. (2018). The anti-anemic effect of dried beet green in phenylhydrazine treated rats. Arch. Pharm. Sci. Ain Shams Univ. 2 (2): 54-69.
[28]	Claro LM, Leonart MSS, Comar SR, do Nascimento AJ. (2006). Effect of vitamins C and E on oxidative processes in human erythrocytes. Cell Biochem. Funct. 24 (6): 531–535.
[29]	Pingali P, Srinivas P, Reddy M. (2015). Study of anti-anaemic effect of Schrebera Swietenioides roxb. In rat models,” Asian J. Pharm. Clin. Res. 8 (5) 305–308.
[30]	Gheith, I, El-Mahmoudy, A. (2018). Laboratory evidence for the hematopoietic potential of Beta vulgaris leaf and stalk extract in a phenylhydrazine model of anemia,” Brazilian J. Med. Biol. Res. Rev. Bras. Pesqui. medicas Biol. 51 (11): 1-8.
[31]	Beshel, FN, Beshel, J A, Ante, E E. (2018). The Ethanolic Extract of Beetroot (Beta Vulgaris) Ameliorates Some Red Cell Parameters In Phenylhydrazine-Induced Anaemic Rats. IOSR J. Nurs. Heal. Sci. 7 (4): 27–30.
[32]	Jinhee, C, So, J B, Areum, K, Nam, H L, Sang-Hee, B, Gi-Ok, K, Youngheun, J. Beetroot (Beta vulgaris) rescues mice from γ-ray irradiation by accelerating hematopoiesis and curtailing immunosuppression. Pharm Biol. 55 (1): 306–316.
[33]	Roy CN, Mak HH, Akpan I, Losyev G, Zurakowski D, Andrews NC. (2007). Hepcidin antimicrobial peptide transgenic mice exhibit features of the anemia of inflammation. Blood. 109 (9) 4038-4044.
[34]	Weinstein DA, Roy CN, Fleming MD, Loda MF, Wolfsdorf JI, Andrews NC. (2002). Inappropriate expression of hepcidin is associated with iron refractory anemia: Implications for the anemia of chronic disease. Blood. 100 (10): 3776–3781.
[35]	Vokurka M, Krijt J, Šulc K, Nečas E. (2006). Hepcidin mRNA levels in mouse liver respond to inhibition of erythropoiesis. Physiol. Res. 55 (6): 667–674.
[36]	Ganz T. (2007). Molecular control of iron transport. J. Am. Soc. Nephrol. 18 (2): 394-400.
[37]	Nwanna EE, Ibukun EO, Oboh G. (2016). Effect of some Tropical Eggplant Fruits (Solanum Spp) Supplemented Diet On Diabetic Neuropathy In Experimental male Wistar Rats In-vivo,. Functional Foods in Health and Disease. 6 (10): 661-676.

Cite This Article

Plain Text BibTeX RIS

APA Style

Safaa Ahmed Salem, Amany Mohammed Gad, Abdellatif Anaam Kamal. (2021). Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats. International Journal of Food Science and Biotechnology, 6(2), 21-29. https://doi.org/10.11648/j.ijfsb.20210602.11

Copy | Download

ACS Style

Safaa Ahmed Salem; Amany Mohammed Gad; Abdellatif Anaam Kamal. Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats. Int. J. Food Sci. Biotechnol. 2021, 6(2), 21-29. doi: 10.11648/j.ijfsb.20210602.11

Copy | Download

AMA Style

Safaa Ahmed Salem, Amany Mohammed Gad, Abdellatif Anaam Kamal. Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats. Int J Food Sci Biotechnol. 2021;6(2):21-29. doi: 10.11648/j.ijfsb.20210602.11

Copy | Download

@article{10.11648/j.ijfsb.20210602.11,
  author = {Safaa Ahmed Salem and Amany Mohammed Gad and Abdellatif Anaam Kamal},
  title = {Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats},
  journal = {International Journal of Food Science and Biotechnology},
  volume = {6},
  number = {2},
  pages = {21-29},
  doi = {10.11648/j.ijfsb.20210602.11},
  url = {https://doi.org/10.11648/j.ijfsb.20210602.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijfsb.20210602.11},
  abstract = {In this study beetroot and eggplant were used as natural source for production of anti-anemic functional food for anemic patients and to prevent it during disease or chemotherapy treatment. Anemic model was induced in albino female rats by daily injection with phenylhydrazin (5mg/kg b.w) for three successive days, then biochemical, hematological and histological parameters were determined including; iron, ferritin, Ascorbic acid, Hepicidin, Hemoglobin and Complete blood count (men corpuscle volume, men corpuscle hemoglobin, haematocriet, red blood cells and white blood cells). Negative control group of normal rats, and positive control group of anemic rats each of six rats were fed on basal diet. Also 24 anemic rats were divided into 4 groups each of 6 rats which were fed on basal diet containing either eggplant or beetroot in ratios of 100, 300g/kg, in addition to 500mg ascorbic acid and 2mg folic acid/ kg diet for all groups for 30 days. feeding period was 30 days. At the end of experiment the above biochemical and haematological parameters were evaluated and the liver and spleen were histopathological examined. Decreases in hemoglobin, men corpuscle volume, haematocriet, red blood cells, men corpuscle hemoglobin, iron, ascorbic acid and ferritin, also increases in white blood cells and hepcidin were observed in anemic model rats. At the end of feeding period with the previous supplemented tested basal diet, increases in all aforementioned parameters, except white blood cells and hepcidin which showed decreases compared to anemic positive group were found.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats
AU  - Safaa Ahmed Salem
AU  - Amany Mohammed Gad
AU  - Abdellatif Anaam Kamal
Y1  - 2021/04/07
PY  - 2021
N1  - https://doi.org/10.11648/j.ijfsb.20210602.11
DO  - 10.11648/j.ijfsb.20210602.11
T2  - International Journal of Food Science and Biotechnology
JF  - International Journal of Food Science and Biotechnology
JO  - International Journal of Food Science and Biotechnology
SP  - 21
EP  - 29
PB  - Science Publishing Group
SN  - 2578-9643
UR  - https://doi.org/10.11648/j.ijfsb.20210602.11
AB  - In this study beetroot and eggplant were used as natural source for production of anti-anemic functional food for anemic patients and to prevent it during disease or chemotherapy treatment. Anemic model was induced in albino female rats by daily injection with phenylhydrazin (5mg/kg b.w) for three successive days, then biochemical, hematological and histological parameters were determined including; iron, ferritin, Ascorbic acid, Hepicidin, Hemoglobin and Complete blood count (men corpuscle volume, men corpuscle hemoglobin, haematocriet, red blood cells and white blood cells). Negative control group of normal rats, and positive control group of anemic rats each of six rats were fed on basal diet. Also 24 anemic rats were divided into 4 groups each of 6 rats which were fed on basal diet containing either eggplant or beetroot in ratios of 100, 300g/kg, in addition to 500mg ascorbic acid and 2mg folic acid/ kg diet for all groups for 30 days. feeding period was 30 days. At the end of experiment the above biochemical and haematological parameters were evaluated and the liver and spleen were histopathological examined. Decreases in hemoglobin, men corpuscle volume, haematocriet, red blood cells, men corpuscle hemoglobin, iron, ascorbic acid and ferritin, also increases in white blood cells and hepcidin were observed in anemic model rats. At the end of feeding period with the previous supplemented tested basal diet, increases in all aforementioned parameters, except white blood cells and hepcidin which showed decreases compared to anemic positive group were found.
VL  - 6
IS  - 2
ER  -

Copy | Download

Author Information

Safaa Ahmed Salem

Department of Medicinal Food, National Organization for Drug Control and Research (NODCAR), Giza, Egypt
Amany Mohammed Gad

Department Pharmacology, National Organization for Drug Control and Research (NODCAR), Giza, Egypt
Abdellatif Anaam Kamal

Department of Medicinal Food, National Organization for Drug Control and Research (NODCAR), Giza, Egypt

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Safaa Ahmed Salem, Amany Mohammed Gad, Abdellatif Anaam Kamal. (2021). Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats. International Journal of Food Science and Biotechnology, 6(2), 21-29. https://doi.org/10.11648/j.ijfsb.20210602.11

Copy | Download

ACS Style

Safaa Ahmed Salem; Amany Mohammed Gad; Abdellatif Anaam Kamal. Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats. Int. J. Food Sci. Biotechnol. 2021, 6(2), 21-29. doi: 10.11648/j.ijfsb.20210602.11

Copy | Download

AMA Style

Safaa Ahmed Salem, Amany Mohammed Gad, Abdellatif Anaam Kamal. Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats. Int J Food Sci Biotechnol. 2021;6(2):21-29. doi: 10.11648/j.ijfsb.20210602.11

Copy | Download

@article{10.11648/j.ijfsb.20210602.11,
  author = {Safaa Ahmed Salem and Amany Mohammed Gad and Abdellatif Anaam Kamal},
  title = {Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats},
  journal = {International Journal of Food Science and Biotechnology},
  volume = {6},
  number = {2},
  pages = {21-29},
  doi = {10.11648/j.ijfsb.20210602.11},
  url = {https://doi.org/10.11648/j.ijfsb.20210602.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijfsb.20210602.11},
  abstract = {In this study beetroot and eggplant were used as natural source for production of anti-anemic functional food for anemic patients and to prevent it during disease or chemotherapy treatment. Anemic model was induced in albino female rats by daily injection with phenylhydrazin (5mg/kg b.w) for three successive days, then biochemical, hematological and histological parameters were determined including; iron, ferritin, Ascorbic acid, Hepicidin, Hemoglobin and Complete blood count (men corpuscle volume, men corpuscle hemoglobin, haematocriet, red blood cells and white blood cells). Negative control group of normal rats, and positive control group of anemic rats each of six rats were fed on basal diet. Also 24 anemic rats were divided into 4 groups each of 6 rats which were fed on basal diet containing either eggplant or beetroot in ratios of 100, 300g/kg, in addition to 500mg ascorbic acid and 2mg folic acid/ kg diet for all groups for 30 days. feeding period was 30 days. At the end of experiment the above biochemical and haematological parameters were evaluated and the liver and spleen were histopathological examined. Decreases in hemoglobin, men corpuscle volume, haematocriet, red blood cells, men corpuscle hemoglobin, iron, ascorbic acid and ferritin, also increases in white blood cells and hepcidin were observed in anemic model rats. At the end of feeding period with the previous supplemented tested basal diet, increases in all aforementioned parameters, except white blood cells and hepcidin which showed decreases compared to anemic positive group were found.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats
AU  - Safaa Ahmed Salem
AU  - Amany Mohammed Gad
AU  - Abdellatif Anaam Kamal
Y1  - 2021/04/07
PY  - 2021
N1  - https://doi.org/10.11648/j.ijfsb.20210602.11
DO  - 10.11648/j.ijfsb.20210602.11
T2  - International Journal of Food Science and Biotechnology
JF  - International Journal of Food Science and Biotechnology
JO  - International Journal of Food Science and Biotechnology
SP  - 21
EP  - 29
PB  - Science Publishing Group
SN  - 2578-9643
UR  - https://doi.org/10.11648/j.ijfsb.20210602.11
AB  - In this study beetroot and eggplant were used as natural source for production of anti-anemic functional food for anemic patients and to prevent it during disease or chemotherapy treatment. Anemic model was induced in albino female rats by daily injection with phenylhydrazin (5mg/kg b.w) for three successive days, then biochemical, hematological and histological parameters were determined including; iron, ferritin, Ascorbic acid, Hepicidin, Hemoglobin and Complete blood count (men corpuscle volume, men corpuscle hemoglobin, haematocriet, red blood cells and white blood cells). Negative control group of normal rats, and positive control group of anemic rats each of six rats were fed on basal diet. Also 24 anemic rats were divided into 4 groups each of 6 rats which were fed on basal diet containing either eggplant or beetroot in ratios of 100, 300g/kg, in addition to 500mg ascorbic acid and 2mg folic acid/ kg diet for all groups for 30 days. feeding period was 30 days. At the end of experiment the above biochemical and haematological parameters were evaluated and the liver and spleen were histopathological examined. Decreases in hemoglobin, men corpuscle volume, haematocriet, red blood cells, men corpuscle hemoglobin, iron, ascorbic acid and ferritin, also increases in white blood cells and hepcidin were observed in anemic model rats. At the end of feeding period with the previous supplemented tested basal diet, increases in all aforementioned parameters, except white blood cells and hepcidin which showed decreases compared to anemic positive group were found.
VL  - 6
IS  - 2
ER  -

Copy | Download