PCR-RFLP Fails to Reveal Variability Within <i>Schistosoma haematobium</i> Detected in Loum (Cameroon) by Isoelectrofocusing Technique

Luogbou Nzu Deguy D’or; Palmer Masumbe Netongo; Nguemaïm Ngoufo Flore; Marc Kenmogne Kouam; Louis-Albert Tchuem-Tchuente; Wilfred Fon Mbacham

doi:doi:10.11648/j.bmb.20210603.11

PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique

Luogbou Nzu Deguy D’or, Palmer Masumbe Netongo, Nguemaïm Ngoufo Flore, Marc Kenmogne Kouam, Louis-Albert Tchuem-Tchuente, Wilfred Fon Mbacham

Published in Biochemistry and Molecular Biology (Volume 6, Issue 3)

Received: 4 June 2021 Accepted: 25 June 2021 Published: 9 July 2021

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Schistosoma haematobium, which causes urinary schistosomiasis in humans is responsible for the largest number of infections in the word. Genetic variability among parasite populations is an important factor in their potential for producing harmful effects on the human populations they infect. In many areas, S. haematobium is sympatric with related schistosome parasites (most of other mammals) (i.e., S. bovis, S. mattheei, S. curassoni, S. intercalatum, S. guineensis and S. margrebowiei). PCR-RFLP analysis of ITS-2 rDNA loci is an usefull tools to detect hybrids amongs Schistosoma haematobium group. Many studies have been carry out in the town of Loum (Cameroon) in order to characterize Schistosoma haematobium species from this locality. However, no study based on PCR-RFLP analysis succeeds to detect any genetic variability as reported before using electrofocusing (IEF) technique. PCR-RFLP analysis realised on 10 isolates of Schistosoma haematobium from Loum reveals a DNA fragment of 501 bp after amplification of ITS2 ribosomal gene. For all the samples, the enzymatic digestion of the mentioned DNA fragment gene with Taq I reveals two DNA bands of 158 bp and 199 bp which is characteristic of Schistosoma haematobium. In summary, molecular characterization of S. haematobium in Loum using PCR-RFLP approach reveals once more the absence of hybrids and no genetic variability. Further studies on a larger geographic scale involving many schools in Loum should be encouraged to screen more parasite isolates with different primers and molecular toolS. Information from such studies would provide better insight into the local lineages of S. haematobium. This knowledge might play a major role in establishing control strategies for urogenital schistosomiasis in Loum.

Published in	Biochemistry and Molecular Biology (Volume 6, Issue 3)
DOI	10.11648/j.bmb.20210603.11
Page(s)	35-39
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Schistosoma haematobium, Genetic Variability, PCR-RFLP, Loum, Cameroon

References

[1]	Molyneux DH, Savioli L, Engels D. (2017). Neglected tropical diseases: progress towards addressing the chronic pandemic. Lancet. 389 (10066): 3 12–325. doi: 10.1016/S0140-6736(16)30171-4.
[2]	King CH, Dickman K, Tisch DJ (2005). Reassessment of the cost of chronic helmintic infection: A meta-analysis of disability-related outcomes in endemic schistosomiasiS. Lancet 365: 1561–1569.
[3]	Van der Werf M J, Bosompem KM, de Vlas SJ (2003) Schistosomiasis control in Ghana: Case management and means for diagnosis and treatment within the health system. Trans R Soc Trop Med Hyg 97: 146–152.
[4]	Lucio Lara S. , Julio Santos, Maria joao G., Carina Bernardo, Carlos Lopes, Gabriel Rinaldi, Paul J. Brindley and José M. Correia da Costa (2021). Urogenital schistosomiasis-History, pathogenesis, and bladder cancer. Journal of Clinical Medecine, 10 (2): 205 Doi: 10.3390/Jcm10020205.
[5]	Sire C, Durand P, Pointier J. P, Theron A, (1999). Genet-ic diversity and recruitment pattern of Schistosoma mansoni in a Biomphalaria glabrata snail popula-tion: a field study using randomly-amplified poly-morphic DNA markerS. Journal of Parasitology, 85: 436–441.
[6]	Mohammed A. Afifi, Asif A. Jiman-Fatani, Nabeel H. Al-Hussainy, Mohammed W. Al-Rabia, Anas A. Bogari (2016). Genetic diversity among natural populations of Schistosoma haematobium might contribute to inconsistent virulence and diverse clinical outcomeS. Journal of Microscopy and Ultrastructure, 4: 222–227.
[7]	Gryseels B, Polman K, Clerinx J, Kestens L. (2006). Human schistosomiasiS. Lancet, 368: 1106–1118.
[8]	Curtis J, Sorensen R E, Kristen P L, Minchella D J, (2001). Microsatellites loci in human blood fluke Schistosoma mansoni and their utility for other Schistosome specieS. Molecular Ecology, 1: 143-145.
[9]	Webster BL, Diaw OT, Seye MM, Webster JP, Rollinson D. (2013) Introgressive Hybridization of Schistosoma haematobium Group Species in Senegal: Species Barrier Break Down between Ruminant and Human SchistosomeS. NeglTrop Dis, 7 (4): e2110. doi: 10.1371/journal.pntd.0002110.
[10]	Tom Pennance, Fiona Allan, Aidan Emery, Muriel Rabone, Jo Cable, Amadou Djirmay Garba, Amina Amadou Hamidou4, Joanne P. Webster, David Rollinson and Bonnie L. Webster (2020). Interactions between Schistosoma haematobium group species and their Bulinus spp. intermediate hosts along the Niger River Valley. Parasites Vectors, 13: 268 https://doi.org/10.1186/s13071-020-04136-9
[11]	Wright C A and Ross G C (1983). Enzymes in Schistosoma haematobium. Bull WHO 61: 307–316.
[12]	Webster BL, Tchuem Tchuenté LA, Southgate VR.(2006) A single-strand conformation polymorphism (SSCP) approach for investigating genetic interactions of Schistosoma haematobium and Schistosoma guineensis in Loum, Cameroon. Parasitology research, DOI 10.1007/s00436-006-0310-0.
[13]	Tchuem Tchuenté LA, Morand S, Imbert-Establet D, Delay B, Jourdane J. (1996) Competitive exclusion in human schistosomes: the puzzling restricted distribution of Schistosoma intercalatum. Parasitology, 113: 129–136.
[14]	Tchuem Tchuenté L A, Southgate V R, Njiokou F, Njiné T, Kouemeni L E, Jourdane J, (1997). The evolution of schistosomiasis at Loum, Cameroon: replacement of Schistosoma intercalatum by S. haematobium through introgressive hybridisation. Transactions of the Royal Society of Tropical Med-icine and Hygiene, 91: 664–665.
[15]	Luogbou Nzu D D, Netongo Masumbe Palmer, Bayemi Pougue Henri, Mbacham Fon Wilfred and Tchuem Tchuente Louis-Albert (2015). Detection of hybrid Schistosoma haematobium group species in Cameroon by PCR-RFLP of the second internal transcribed spacer (ITS-2). Word journal of pharmaceutical Research 4 (6): 1961-1980.
[16]	Tchuem Tchuenté LA, Behnke JM, Gilbert FS, Southgate VR, Vercruysse J. (2003). Polyparasitism with Schistosoma haematobium and soil-transmitted helminth infections among school children in Loum, Cameroon Trop Med Int Health. 8 (11): 975-86.
[17]	Southgate Vaughan R, Van Wijk Henry B, Wright Carol A. (1976). Schistosomiasis at Loum, Cameroun: Schistosoma haematobium, S. intercalatum and their natural hybrid Z Parasitenkd. Apr 30; 49 (2): 145-59.
[18]	Christensen NØ, Gøtsche G, Frandsen F (1984). Parasitological Techniques for Use in Routine Laboratory Maintenance of Schistosomes and for Use in Studies on the Epidemiology of Human and Bovine SchistosomiasiS. , Danish Bilharziasis Laboratory, Charlottenlund, WHO Collaborating Centre for Applied Malacology and Schistosomiasis Control. 1984; 2-10.
[19]	Barber E, Mkoji GM, Loker ES (2000). PCR-RFLP analysis of the ITS 2 region to identify Schistosoma haematobium and S. bovis from Kenya. Am J Trop Med Hyg, 62 (4): 434-440.
[20]	Luogbou Nzu D. D., Netongo Palmer M., Mbacham Wilfred F. and Tchuem Tchuente Louis-Albert (2013). Preliminary studies on the variability of Schistosoma haematobium Glucose-6-Phosphate Dehydrogenase by Isoelectric Focusing. Cameroon Journal of Biological and Biochemical Sciences, 21 (1): 21-26.
[21]	Webster B. L., Southgate V. R., Tchuem Tchuenté L. A. (2003). Isoenzyme analysis of Schistosoma haematobium, S. intercalatum and their hybrids and occurrences of natural hybridisation in Cameroon. Journal of Helminthology, 77: 269–274.
[22]	Gower CM, Gouvras AN, Lamberton PH, et al (2013). Population genetic structure of Schistosoma mansoni and Schistosoma haematobium from across six sub-Saharan African countries: implications for epidemiology, evolution and control. Acta Trop. 128 (2): 261–274. doi: 10.1016/j.actatropica.2012.09.014.

Cite This Article

Plain Text BibTeX RIS

APA Style

Luogbou Nzu Deguy D’or, Palmer Masumbe Netongo, Nguemaïm Ngoufo Flore, Marc Kenmogne Kouam, Louis-Albert Tchuem-Tchuente, et al. (2021). PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique. Biochemistry and Molecular Biology, 6(3), 35-39. https://doi.org/10.11648/j.bmb.20210603.11

Copy | Download

ACS Style

Luogbou Nzu Deguy D’or; Palmer Masumbe Netongo; Nguemaïm Ngoufo Flore; Marc Kenmogne Kouam; Louis-Albert Tchuem-Tchuente, et al. PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique. Biochem. Mol. Biol. 2021, 6(3), 35-39. doi: 10.11648/j.bmb.20210603.11

Copy | Download

AMA Style

Luogbou Nzu Deguy D’or, Palmer Masumbe Netongo, Nguemaïm Ngoufo Flore, Marc Kenmogne Kouam, Louis-Albert Tchuem-Tchuente, et al. PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique. Biochem Mol Biol. 2021;6(3):35-39. doi: 10.11648/j.bmb.20210603.11

Copy | Download

@article{10.11648/j.bmb.20210603.11,
  author = {Luogbou Nzu Deguy D’or and Palmer Masumbe Netongo and Nguemaïm Ngoufo Flore and Marc Kenmogne Kouam and Louis-Albert Tchuem-Tchuente and Wilfred Fon Mbacham},
  title = {PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique},
  journal = {Biochemistry and Molecular Biology},
  volume = {6},
  number = {3},
  pages = {35-39},
  doi = {10.11648/j.bmb.20210603.11},
  url = {https://doi.org/10.11648/j.bmb.20210603.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20210603.11},
  abstract = {Schistosoma haematobium, which causes urinary schistosomiasis in humans is responsible for the largest number of infections in the word. Genetic variability among parasite populations is an important factor in their potential for producing harmful effects on the human populations they infect. In many areas, S. haematobium is sympatric with related schistosome parasites (most of other mammals) (i.e., S. bovis, S. mattheei, S. curassoni, S. intercalatum, S. guineensis and S. margrebowiei). PCR-RFLP analysis of ITS-2 rDNA loci is an usefull tools to detect hybrids amongs Schistosoma haematobium group. Many studies have been carry out in the town of Loum (Cameroon) in order to characterize Schistosoma haematobium species from this locality. However, no study based on PCR-RFLP analysis succeeds to detect any genetic variability as reported before using electrofocusing (IEF) technique. PCR-RFLP analysis realised on 10 isolates of Schistosoma haematobium from Loum reveals a DNA fragment of 501 bp after amplification of ITS2 ribosomal gene. For all the samples, the enzymatic digestion of the mentioned DNA fragment gene with Taq I reveals two DNA bands of 158 bp and 199 bp which is characteristic of Schistosoma haematobium. In summary, molecular characterization of S. haematobium in Loum using PCR-RFLP approach reveals once more the absence of hybrids and no genetic variability. Further studies on a larger geographic scale involving many schools in Loum should be encouraged to screen more parasite isolates with different primers and molecular toolS. Information from such studies would provide better insight into the local lineages of S. haematobium. This knowledge might play a major role in establishing control strategies for urogenital schistosomiasis in Loum.},
 year = {2021}
}

Copy | Download

TY - JOUR
T1 - PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique
AU - Luogbou Nzu Deguy D’or
AU - Palmer Masumbe Netongo
AU - Nguemaïm Ngoufo Flore
AU - Marc Kenmogne Kouam
AU - Louis-Albert Tchuem-Tchuente
AU - Wilfred Fon Mbacham
Y1 - 2021/07/09
PY - 2021
N1 - https://doi.org/10.11648/j.bmb.20210603.11
DO - 10.11648/j.bmb.20210603.11
T2 - Biochemistry and Molecular Biology
JF - Biochemistry and Molecular Biology
JO - Biochemistry and Molecular Biology
SP - 35
EP - 39
PB - Science Publishing Group
SN - 2575-5048
UR - https://doi.org/10.11648/j.bmb.20210603.11
AB - Schistosoma haematobium, which causes urinary schistosomiasis in humans is responsible for the largest number of infections in the word. Genetic variability among parasite populations is an important factor in their potential for producing harmful effects on the human populations they infect. In many areas, S. haematobium is sympatric with related schistosome parasites (most of other mammals) (i.e., S. bovis, S. mattheei, S. curassoni, S. intercalatum, S. guineensis and S. margrebowiei). PCR-RFLP analysis of ITS-2 rDNA loci is an usefull tools to detect hybrids amongs Schistosoma haematobium group. Many studies have been carry out in the town of Loum (Cameroon) in order to characterize Schistosoma haematobium species from this locality. However, no study based on PCR-RFLP analysis succeeds to detect any genetic variability as reported before using electrofocusing (IEF) technique. PCR-RFLP analysis realised on 10 isolates of Schistosoma haematobium from Loum reveals a DNA fragment of 501 bp after amplification of ITS2 ribosomal gene. For all the samples, the enzymatic digestion of the mentioned DNA fragment gene with Taq I reveals two DNA bands of 158 bp and 199 bp which is characteristic of Schistosoma haematobium. In summary, molecular characterization of S. haematobium in Loum using PCR-RFLP approach reveals once more the absence of hybrids and no genetic variability. Further studies on a larger geographic scale involving many schools in Loum should be encouraged to screen more parasite isolates with different primers and molecular toolS. Information from such studies would provide better insight into the local lineages of S. haematobium. This knowledge might play a major role in establishing control strategies for urogenital schistosomiasis in Loum.
VL - 6
IS - 3
ER -

Copy | Download

Author Information

Luogbou Nzu Deguy D’or

Centre for Schistosomiasis & Parasitology, Yaounde, Cameroon
Palmer Masumbe Netongo

The Laboratory for Public Health Research Biotechnologies, University of Yaounde I, Yaounde, Cameroon
Nguemaïm Ngoufo Flore
Marc Kenmogne Kouam

Department of Animal Sciences, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang, Cameroon
Louis-Albert Tchuem-Tchuente

Centre for Schistosomiasis & Parasitology, Yaounde, Cameroon
Wilfred Fon Mbacham

The Laboratory for Public Health Research Biotechnologies, University of Yaounde I, Yaounde, Cameroon

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Luogbou Nzu Deguy D’or, Palmer Masumbe Netongo, Nguemaïm Ngoufo Flore, Marc Kenmogne Kouam, Louis-Albert Tchuem-Tchuente, et al. (2021). PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique. Biochemistry and Molecular Biology, 6(3), 35-39. https://doi.org/10.11648/j.bmb.20210603.11

Copy | Download

ACS Style

Luogbou Nzu Deguy D’or; Palmer Masumbe Netongo; Nguemaïm Ngoufo Flore; Marc Kenmogne Kouam; Louis-Albert Tchuem-Tchuente, et al. PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique. Biochem. Mol. Biol. 2021, 6(3), 35-39. doi: 10.11648/j.bmb.20210603.11

Copy | Download

AMA Style

Luogbou Nzu Deguy D’or, Palmer Masumbe Netongo, Nguemaïm Ngoufo Flore, Marc Kenmogne Kouam, Louis-Albert Tchuem-Tchuente, et al. PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique. Biochem Mol Biol. 2021;6(3):35-39. doi: 10.11648/j.bmb.20210603.11

Copy | Download

@article{10.11648/j.bmb.20210603.11,
  author = {Luogbou Nzu Deguy D’or and Palmer Masumbe Netongo and Nguemaïm Ngoufo Flore and Marc Kenmogne Kouam and Louis-Albert Tchuem-Tchuente and Wilfred Fon Mbacham},
  title = {PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique},
  journal = {Biochemistry and Molecular Biology},
  volume = {6},
  number = {3},
  pages = {35-39},
  doi = {10.11648/j.bmb.20210603.11},
  url = {https://doi.org/10.11648/j.bmb.20210603.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20210603.11},
  abstract = {Schistosoma haematobium, which causes urinary schistosomiasis in humans is responsible for the largest number of infections in the word. Genetic variability among parasite populations is an important factor in their potential for producing harmful effects on the human populations they infect. In many areas, S. haematobium is sympatric with related schistosome parasites (most of other mammals) (i.e., S. bovis, S. mattheei, S. curassoni, S. intercalatum, S. guineensis and S. margrebowiei). PCR-RFLP analysis of ITS-2 rDNA loci is an usefull tools to detect hybrids amongs Schistosoma haematobium group. Many studies have been carry out in the town of Loum (Cameroon) in order to characterize Schistosoma haematobium species from this locality. However, no study based on PCR-RFLP analysis succeeds to detect any genetic variability as reported before using electrofocusing (IEF) technique. PCR-RFLP analysis realised on 10 isolates of Schistosoma haematobium from Loum reveals a DNA fragment of 501 bp after amplification of ITS2 ribosomal gene. For all the samples, the enzymatic digestion of the mentioned DNA fragment gene with Taq I reveals two DNA bands of 158 bp and 199 bp which is characteristic of Schistosoma haematobium. In summary, molecular characterization of S. haematobium in Loum using PCR-RFLP approach reveals once more the absence of hybrids and no genetic variability. Further studies on a larger geographic scale involving many schools in Loum should be encouraged to screen more parasite isolates with different primers and molecular toolS. Information from such studies would provide better insight into the local lineages of S. haematobium. This knowledge might play a major role in establishing control strategies for urogenital schistosomiasis in Loum.},
 year = {2021}
}

Copy | Download