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Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride

Received: 27 May 2020     Accepted: 15 June 2020     Published: 4 March 2021
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Abstract

Sida corymbosa plant is one of medicinal plants used in many parts of Africa, including Nigeria for the treatment of human diseases. The purpose of this work was to investigate the antioxidant effects of Sida corymbosa ethanolic leaf extract on rats administered carbon tetrachloride (CCl4). One hundred and sixty male-rats between 170-180 g were used. They were grouped into 8 major groups (groups A-H) and 4 subgroups (A1-H4) in each group making it 32 subgroups of 5 rats each. The major groups comprise A (positive control- normal rats giving feed and water only and left for 7-28 d), B (negative control-those administered CCl4 only at 0.4 ml/kgbw and left for 7-28 d), C-H (treatment groups-those administered extract before and after CCl4 administration at 5,000, 3,000 and 1,000 mg/kgbw for 7-28 d). Antioxidant assays were determined using UV-VIS spectrophotometer (model 752g, China). Results of serum antioxidant assays done revealed that levels of serum reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), supraoxide dismutase (SOD) and total antioxidant capacity (TAC) increased significantly (P<0.05) in the treatment groups from 7-28 d, when compared with the negative control. Oxidative assay carried out showed that serum malondialdehyde (MDA) levels were significantly lower (P<0.05) in treatment groups than in negative control groups. These may be suggesting that the extract may have improved the antioxidant levels of the rats. Sida corymbosa ethanolic leaf extract may, therefore, have positive effects on antioxidant status of rats administered CCl4.

Published in American Journal of Biomedical and Life Sciences (Volume 9, Issue 2)
DOI 10.11648/j.ajbls.20210902.11
Page(s) 97-110
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Sida Species, Antioxidant Results in Rats, Oxidative Stress

References
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  • APA Style

    Charles Chijioke Dike, Francis Chukwuemeka Ezeonu, Chinenye Enoch Oguazu, Emmanuel Nonso Ezeokafor, Hugh Clifford Chima Maduka, et al. (2021). Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride. American Journal of Biomedical and Life Sciences, 9(2), 97-110. https://doi.org/10.11648/j.ajbls.20210902.11

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    ACS Style

    Charles Chijioke Dike; Francis Chukwuemeka Ezeonu; Chinenye Enoch Oguazu; Emmanuel Nonso Ezeokafor; Hugh Clifford Chima Maduka, et al. Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride. Am. J. Biomed. Life Sci. 2021, 9(2), 97-110. doi: 10.11648/j.ajbls.20210902.11

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    AMA Style

    Charles Chijioke Dike, Francis Chukwuemeka Ezeonu, Chinenye Enoch Oguazu, Emmanuel Nonso Ezeokafor, Hugh Clifford Chima Maduka, et al. Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride. Am J Biomed Life Sci. 2021;9(2):97-110. doi: 10.11648/j.ajbls.20210902.11

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  • @article{10.11648/j.ajbls.20210902.11,
      author = {Charles Chijioke Dike and Francis Chukwuemeka Ezeonu and Chinenye Enoch Oguazu and Emmanuel Nonso Ezeokafor and Hugh Clifford Chima Maduka and Aloysius Ngozika Okpogba and Akpoghene Eyeghre Onoriode and Sunday Jacob Asebioyo and Emilia Chika Nwankwo and Ebelechukwu Nwaoma Ihekwoaba and Aronu Cecilia Nkechi},
      title = {Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {9},
      number = {2},
      pages = {97-110},
      doi = {10.11648/j.ajbls.20210902.11},
      url = {https://doi.org/10.11648/j.ajbls.20210902.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20210902.11},
      abstract = {Sida corymbosa plant is one of medicinal plants used in many parts of Africa, including Nigeria for the treatment of human diseases. The purpose of this work was to investigate the antioxidant effects of Sida corymbosa ethanolic leaf extract on rats administered carbon tetrachloride (CCl4). One hundred and sixty male-rats between 170-180 g were used. They were grouped into 8 major groups (groups A-H) and 4 subgroups (A1-H4) in each group making it 32 subgroups of 5 rats each. The major groups comprise A (positive control- normal rats giving feed and water only and left for 7-28 d), B (negative control-those administered CCl4 only at 0.4 ml/kgbw and left for 7-28 d), C-H (treatment groups-those administered extract before and after CCl4 administration at 5,000, 3,000 and 1,000 mg/kgbw for 7-28 d). Antioxidant assays were determined using UV-VIS spectrophotometer (model 752g, China). Results of serum antioxidant assays done revealed that levels of serum reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), supraoxide dismutase (SOD) and total antioxidant capacity (TAC) increased significantly (PSida corymbosa ethanolic leaf extract may, therefore, have positive effects on antioxidant status of rats administered CCl4.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride
    AU  - Charles Chijioke Dike
    AU  - Francis Chukwuemeka Ezeonu
    AU  - Chinenye Enoch Oguazu
    AU  - Emmanuel Nonso Ezeokafor
    AU  - Hugh Clifford Chima Maduka
    AU  - Aloysius Ngozika Okpogba
    AU  - Akpoghene Eyeghre Onoriode
    AU  - Sunday Jacob Asebioyo
    AU  - Emilia Chika Nwankwo
    AU  - Ebelechukwu Nwaoma Ihekwoaba
    AU  - Aronu Cecilia Nkechi
    Y1  - 2021/03/04
    PY  - 2021
    N1  - https://doi.org/10.11648/j.ajbls.20210902.11
    DO  - 10.11648/j.ajbls.20210902.11
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 97
    EP  - 110
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20210902.11
    AB  - Sida corymbosa plant is one of medicinal plants used in many parts of Africa, including Nigeria for the treatment of human diseases. The purpose of this work was to investigate the antioxidant effects of Sida corymbosa ethanolic leaf extract on rats administered carbon tetrachloride (CCl4). One hundred and sixty male-rats between 170-180 g were used. They were grouped into 8 major groups (groups A-H) and 4 subgroups (A1-H4) in each group making it 32 subgroups of 5 rats each. The major groups comprise A (positive control- normal rats giving feed and water only and left for 7-28 d), B (negative control-those administered CCl4 only at 0.4 ml/kgbw and left for 7-28 d), C-H (treatment groups-those administered extract before and after CCl4 administration at 5,000, 3,000 and 1,000 mg/kgbw for 7-28 d). Antioxidant assays were determined using UV-VIS spectrophotometer (model 752g, China). Results of serum antioxidant assays done revealed that levels of serum reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), supraoxide dismutase (SOD) and total antioxidant capacity (TAC) increased significantly (PSida corymbosa ethanolic leaf extract may, therefore, have positive effects on antioxidant status of rats administered CCl4.
    VL  - 9
    IS  - 2
    ER  - 

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Author Information
  • Department of Human Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria

  • Department of Applied Biochemistry, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Nigeria

  • Department of Applied Biochemistry, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Nigeria

  • Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria

  • Department of Human Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria

  • Department of Human Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria

  • Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria

  • Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria

  • Department of Human Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria

  • Department of Environmental Health Sciences, Faculty of Health Sciences and Technology, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria

  • Department of Environmental Health Sciences, Faculty of Health Sciences and Technology, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria

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