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Identification of Blood Meal of Sand Flies in a Cutaneous Leishmaniasis Endemic Area, Volta Region-Ghana
American Journal of Biomedical and Life Sciences
Volume 8, Issue 4, August 2020, Pages: 69-75
Received: May 28, 2020; Accepted: Jun. 10, 2020; Published: Jun. 20, 2020
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Edna Dzifa Doe, Department of Biomedical Sciences, University of Cape Coast, Cape Coast, Ghana; Cellular and Clinical Research Centre, Radiological and Medical Sciences Research Institute, Accra, Ghana
Godwin Kwakye-Nuako, Department of Biomedical Sciences, University of Cape Coast, Cape Coast, Ghana
Alexander Egyir-Yawson, Department of Biomedical Sciences, University of Cape Coast, Cape Coast, Ghana
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Background: Vector incrimination is of utmost importance in the transmission of Leishmania species identified in the Volta region of Ghana. The feeding preference of sand flies provides valuable information about the vector-host interactions, including reservoir host. Objective: The main aim of this study was to identify the blood meal in collected sand flies and its source from three villages in a cutaneous leishmaniasis endemic area in the Ho Municipality. Method: Sand flies was collected using CDC light straps and sticky paper traps from Lume Atsiame, Dodome Dogblome and Dodome Awuiasu. They were morphologically identified by taxonomic keys. DNA was extracted from the individual sand flies using the potassium acetate extraction method. Polymerase chain reaction (PCR) amplification of cytochrome b gene (cyt b) fragment was carried out and subsequently DNA sequenced. Results: Of a total of three hundred and sixty-three (363) female sand flies that were analysed, eighty-four (84) representing 23.14% were positive for blood meal presence with an amplified DNA of the expected size, 359bp. Out of the number that was sequenced, the cyt b sequencing revealed that four (4) blood fed females fed on bloodmeal from human (Homo sapiens) and house mouse (Mus musculus). Conclusion: The blood meal presence was more predominant in Sergentomyia ghesquierei and least in Sergentomyia schwetzi. This may aid in the development of effective strategies to control cutaneous leishmaniasis in the endemic areas.
Cytochrome B Gene, Feeding Preferences, Identification, Polymerase Chain Reaction, Sequencing
To cite this article
Edna Dzifa Doe, Godwin Kwakye-Nuako, Alexander Egyir-Yawson, Identification of Blood Meal of Sand Flies in a Cutaneous Leishmaniasis Endemic Area, Volta Region-Ghana, American Journal of Biomedical and Life Sciences. Vol. 8, No. 4, 2020, pp. 69-75. doi: 10.11648/j.ajbls.20200804.12
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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