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Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria
American Journal of Biomedical and Life Sciences
Volume 8, Issue 4, August 2020, Pages: 64-68
Received: Mar. 18, 2020; Accepted: Apr. 14, 2020; Published: Jun. 9, 2020
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Authors
Garba Ibrahim, Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria
Umar Asiya Imam, Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria
Ganau Ahmed Mohammed, Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria
Raji Mudasiru Iyanda Omowale, Department of Pharmaceutics and Pharm, Microbiology, Usmanu Danfodiyo University, Sokoto, Nigeria
Fana Sani Abdullahi, Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria
Shinkafi Sa’adatu Aliyu, Department of Biological Sciences, Federal University, Gusau, Nigeria
Kazeem Ademola, Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria
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Abstract
Malaria is a serious public health problem, this study was aimed at comparing Microscopy and Rapid Diagnostics test in malaria parasite detection in patients in some hospitals in Sokoto, Nigeria. Blood Samples were collected by venipuncture and dispensed into EDTA bottle. Thick blood films were made by adding few drops of anticoagulated blood on a clean glass slides and emulsifying to coin size using a spreader and allowed to air-dry and stained with 10% Geimsa stain for 10 minutes, allowed to air-dry and examined using ×100 objective to confirm the presence of malaria parasites. Of the 100 patients screened, 37% and 24% were positive for malaria using Carestart and SD-Bioline, while 53% were malaria positive by microscopy. Prevalence of malaria parasite by sex was 53.6% and 52.3% in females and males. Malaria prevalence by age was highest among patients aged 11-20, while the lowest prevalence was observed in patients aged 51-60 years. Based on occupation, the prevalence of malaria was highest among unemployed patients 58.5%, followed by 22.6% prevalence of malaria among business men and women. No significant difference was observed in malaria infection by sex, age and occupations P<0.05. The sensitivity and specificity of Carestart and SD-Bioline screening kits were 54.7% and 82.6%, 34.6 and 87.2%. The predictive positive value of Carestart was 78.4% while its negative predictive value was 61.3% in contrast to SD-Bioline with 75% and 54.7% as the positive predictive value and negative predictive value. Rapid diagnostic test are suitable alternatives to microscopy particularly in resources limited rural areas.
Keywords
Comparison, Microscopic, Rapid Test, Malaria, Sokoto
To cite this article
Garba Ibrahim, Umar Asiya Imam, Ganau Ahmed Mohammed, Raji Mudasiru Iyanda Omowale, Fana Sani Abdullahi, Shinkafi Sa’adatu Aliyu, Kazeem Ademola, Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria, American Journal of Biomedical and Life Sciences. Vol. 8, No. 4, 2020, pp. 64-68. doi: 10.11648/j.ajbls.20200804.11
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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