American Journal of Biomedical and Life Sciences

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Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E

Received: 11 April 2017    Accepted: 23 May 2017    Published: 18 October 2017
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Abstract

The metabolism of ethanol is directly related to oxidative stress, and its ingestion leads to the formation of reactive oxygen species (ROS) such as hydroxyl radicals, superoxide and hydrogen peroxide. Vitamin E has been widely used as an antioxidant; when administered in large doses it is deposited in the liver and then excreted in the bile, urine and feces. The objective of the present study was to evaluate the rate of excretion of fecal vitamin E in relation to its concentrations in serum and liver, and its role as a protective antioxidant against DNA damage induced by acute ethanol consumption. Wistar rats were divided into four groups receiving food and water ad libitum for 4 days plus the following treatments: Control (CG, n = 10 ) no treatment; Ethanol (ET, n = 10 ), receiving an acute ethanol dose intraperitoneally in the amount of 5 g/kg; vitamin E (VE, n = 10) receiving a high oral dose of vitamin E within the first three days in the amount of 100 tocopherol mg/kg body weight; ethanol plus vitamin E (VE + ET, n = 10 ) receiving both the ethanol and vitamin E doses. Higher concentrations of vitamin E were observed in the blood and liver of the animals in the groups that received vitamin E supplementation, independent of the presence or absence of ethanol. Concomitantly, these groups were also those with the highest concentration of the vitamin in the stool. The rate of DNA damage was higher in the groups that received ethanol with or without supplemental vitamin E. However, the rate of damage was lower in the group that received vitamin E supplementation than in the group that did not. The present results show that vitamin E has a protective effect against DNA damage induced by ethanol by reducing the extent of DNA damage.

DOI 10.11648/j.ajbls.20170505.11
Published in American Journal of Biomedical and Life Sciences (Volume 5, Issue 5, October 2017)
Page(s) 88-91
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Vitamin E, Ethanol, Antioxidants, DNA Damage, Supplementation

References
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Author Information
  • Division of Nutrition, Department of Internal Medicine, Medical School of Ribeir?o Preto, University of S?o Paulo, Ribeir?o Preto, Brazil

  • Division of Nutrition, Department of Internal Medicine, Medical School of Ribeir?o Preto, University of S?o Paulo, Ribeir?o Preto, Brazil

  • Nucleus of Research in Sciences and Technology, University of Franca, Franca, Brazil

  • Department of Nutrition, Federal University of Triangulo Mineiro, Uberaba, Brazil

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    Alceu Afonso Jordao, Thais Helena Monteiro, Raquel Alves dos Santos, Guilherme Vannucchi Portari. (2017). Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E. American Journal of Biomedical and Life Sciences, 5(5), 88-91. https://doi.org/10.11648/j.ajbls.20170505.11

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    ACS Style

    Alceu Afonso Jordao; Thais Helena Monteiro; Raquel Alves dos Santos; Guilherme Vannucchi Portari. Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E. Am. J. Biomed. Life Sci. 2017, 5(5), 88-91. doi: 10.11648/j.ajbls.20170505.11

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    AMA Style

    Alceu Afonso Jordao, Thais Helena Monteiro, Raquel Alves dos Santos, Guilherme Vannucchi Portari. Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E. Am J Biomed Life Sci. 2017;5(5):88-91. doi: 10.11648/j.ajbls.20170505.11

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  • @article{10.11648/j.ajbls.20170505.11,
      author = {Alceu Afonso Jordao and Thais Helena Monteiro and Raquel Alves dos Santos and Guilherme Vannucchi Portari},
      title = {Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {5},
      number = {5},
      pages = {88-91},
      doi = {10.11648/j.ajbls.20170505.11},
      url = {https://doi.org/10.11648/j.ajbls.20170505.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajbls.20170505.11},
      abstract = {The metabolism of ethanol is directly related to oxidative stress, and its ingestion leads to the formation of reactive oxygen species (ROS) such as hydroxyl radicals, superoxide and hydrogen peroxide. Vitamin E has been widely used as an antioxidant; when administered in large doses it is deposited in the liver and then excreted in the bile, urine and feces. The objective of the present study was to evaluate the rate of excretion of fecal vitamin E in relation to its concentrations in serum and liver, and its role as a protective antioxidant against DNA damage induced by acute ethanol consumption. Wistar rats were divided into four groups receiving food and water ad libitum for 4 days plus the following treatments: Control (CG, n = 10 ) no treatment; Ethanol (ET, n = 10 ), receiving an acute ethanol dose intraperitoneally in the amount of 5 g/kg; vitamin E (VE, n = 10) receiving a high oral dose of vitamin E within the first three days in the amount of 100 tocopherol mg/kg body weight; ethanol plus vitamin E (VE + ET, n = 10 ) receiving both the ethanol and vitamin E doses. Higher concentrations of vitamin E were observed in the blood and liver of the animals in the groups that received vitamin E supplementation, independent of the presence or absence of ethanol. Concomitantly, these groups were also those with the highest concentration of the vitamin in the stool. The rate of DNA damage was higher in the groups that received ethanol with or without supplemental vitamin E. However, the rate of damage was lower in the group that received vitamin E supplementation than in the group that did not. The present results show that vitamin E has a protective effect against DNA damage induced by ethanol by reducing the extent of DNA damage.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E
    AU  - Alceu Afonso Jordao
    AU  - Thais Helena Monteiro
    AU  - Raquel Alves dos Santos
    AU  - Guilherme Vannucchi Portari
    Y1  - 2017/10/18
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ajbls.20170505.11
    DO  - 10.11648/j.ajbls.20170505.11
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 88
    EP  - 91
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20170505.11
    AB  - The metabolism of ethanol is directly related to oxidative stress, and its ingestion leads to the formation of reactive oxygen species (ROS) such as hydroxyl radicals, superoxide and hydrogen peroxide. Vitamin E has been widely used as an antioxidant; when administered in large doses it is deposited in the liver and then excreted in the bile, urine and feces. The objective of the present study was to evaluate the rate of excretion of fecal vitamin E in relation to its concentrations in serum and liver, and its role as a protective antioxidant against DNA damage induced by acute ethanol consumption. Wistar rats were divided into four groups receiving food and water ad libitum for 4 days plus the following treatments: Control (CG, n = 10 ) no treatment; Ethanol (ET, n = 10 ), receiving an acute ethanol dose intraperitoneally in the amount of 5 g/kg; vitamin E (VE, n = 10) receiving a high oral dose of vitamin E within the first three days in the amount of 100 tocopherol mg/kg body weight; ethanol plus vitamin E (VE + ET, n = 10 ) receiving both the ethanol and vitamin E doses. Higher concentrations of vitamin E were observed in the blood and liver of the animals in the groups that received vitamin E supplementation, independent of the presence or absence of ethanol. Concomitantly, these groups were also those with the highest concentration of the vitamin in the stool. The rate of DNA damage was higher in the groups that received ethanol with or without supplemental vitamin E. However, the rate of damage was lower in the group that received vitamin E supplementation than in the group that did not. The present results show that vitamin E has a protective effect against DNA damage induced by ethanol by reducing the extent of DNA damage.
    VL  - 5
    IS  - 5
    ER  - 

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