Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility

Irina Ivanovna Shilnikova; Irina Aleksandrovna Kluchnikova; Inna Vasilyevna Tereshchenko; Natalia Vladimirovna Dmitrieva

doi:doi:10.11648/j.ajbls.20170504.16

Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility

Irina Ivanovna Shilnikova, Irina Aleksandrovna Kluchnikova, Inna Vasilyevna Tereshchenko, Natalia Vladimirovna Dmitrieva

Published in American Journal of Biomedical and Life Sciences (Volume 5, Issue 4)

Received: 20 February 2017 Accepted: 16 March 2017 Published: 18 October 2017

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

We investigated the possibilities of the MALDI-TOF MS for species identification of anaerobic gram-negative cocci isolated from clinical specimens of cancer patients. A total 70 Veillonella clinical isolates and one Acidaminococcus intestini isolate were analysed by the Bruker Microflex MALDI-TOF instrument with the Biotyper 3, 0 software. All isolates were identified to the species level with a scores greater than 1.9. The most common species were V. parvula (37 strains), then followed by decreasing the frequency V. dispar (16), V. atypica (16) and V. denticariosi (1). Susceptibilities of the isolates were determined by the E-test methodology. All Veillonella isolates were susceptible to imipenem, whereas a high resistance rates were observed for penicillin G, amoxicillin/clavulanate and metronidazole. The proportion of intermediate/resistant isolates of V. parvula, V. dispar and V. atypica to penicillin (MIC ≥ 1 µg/ml) was 86%, 85% and 100%, respectively. The resistance to amoxicillin/clavulanate (MIC 16 - 32 µg/ml) was observed in about 28,6% V. parvula isolates, 23,1% V. dispar isolates and 6,7% V. atypica isolates. According to EUCAST criteria, resistance to metronidazole (MIC ≥ 8 µg/ml) of V. parvula, V. dispar and V. atypica was 88,6%, 53,8% and 40%, respectively.

Published in	American Journal of Biomedical and Life Sciences (Volume 5, Issue 4)
DOI	10.11648/j.ajbls.20170504.16
Page(s)	82-87
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Veillonella Clinical Isolates, Anaerobic Infections, MALDI-TOF MS, Antimicrobial Susceptibility, Resistance Rates, Cancer Patients

References

[1]	Fisher R. G., Denison M. R. Veillonella parvula bacteremia without underlying source. J. Clin. Microbiol. 1996; 34: 3235-3236.
[2]	Liu J. W., Wu J. J., Wang L. R., Teng L. J., Huang T. C. Two fatal cases of Veillonella bacteremia. Eur. J. Clin. Microbiol. Dis. 1998, 17: 62-64.
[3]	Strach M., Siedlar M., Kowalczyk D., Zembala M., Grodzicki T. Sepsis caused by Veillonella parvula infection in a 17-year-old patient with X-linked agammaglobulinemia (Bruton's disease). J. Clin. Microbiol. 2006, 44: 2655-2656.
[4]	Boo T. W., Cryan B., O'Donnell A., Fahy G. Prosthetic valve endocarditis caused Veillonella parvula. J. Infect. 2005, 50: 81-83.
[5]	Marriott D., Stark D., Harkness J. Veillonella parvula discitis and secondary bacteremia: a rare infection complicating endoscopy and colonoscopy? J. Clin. Microbiol. 2007, 45: 672-674.
[6]	Bhatti M. A., Frank M. O. Veillonella parvula meningitis: case report and review of Veillonella infections. Clin. Infect. Dis. 2000, 31: 839-840.
[7]	Shah A., Panjabi C., Nair V., Chaudhry R., Thukral S. S. Veillonella as a cause of chronic anaerobic pneumonitis. Int. J. Infect. Dis. 2008, 12: e115-e117.
[8]	Marchandin H., Teyssier C., de Buochberg M. S., Jean-Pierre H., Carriere C., Jumas-Bilak E. Intra-chromosomal heterogeneity between the four 16S rRNA gene copies in the genus Veillonella: implications for phylogeny and taxonomy. Microbiology 2003, 149: 1493-1501.
[9]	Mashima I., F. Nakazawa. Identification of Veillonella tobetsuensis in tongue biofilm by using a species-specific primer pair. Anaerobe, 2013, 22: 77-81.
[10]	Kostrzewa M., S. Schubert. MALDI-TOF mass spectrometry in microbiology. Caister Academic Press, 2016, p. 200.
[11]	Justesen U. S., Holm A., Knudsen E., Andersen L. B., Jensen T. G., Kemp M., et. al. Species identification of clinical isolates of anaerobic bacteria: a comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry systems. J. Clin. Microbiol. 2011, 49: 4314-4318.
[12]	Fournier R., F. Wallet, B. Grandbastein, L. Dubreuil, R. Courcol, C. Neut, R. Dessein. Chemical extraction versus direct smear for MALDI-TOF mass spectrometry identification of anaerobic bacteria. Anaerobe, 2012, 18: 294-297.
[13]	Barreau M., I. Pagnier, B. La Scola. Improving the identification of anaerobes in the clinical microbiology laboratory through MALDI-TOF mass spectrometry. Anaerobe, 2013, 22: 123-125.
[14]	Fedorko D. P., S. Drake, F. Stock, P. R. Murray. Identification of clinical isolates of anaerobic bacteria using matrix-assisted laser desorption ionization-time of flight mass spectrometry. Eur. J. Clin. Microbiol. Infect. Dis., 2012, 31: 2257-2262.
[15]	Schmitt B. H., S. A. Cunningham, A. L. Dailey, D. R. Gustafson, R. Patel. Identification of anaerobic bacteria by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry with on-plate formic acid preparation. J. Clin. Microbiol., 2013, 51: 782-786.
[16]	Veloo A. C. M., P. E. Elgersma, A. W. Friedrich, E. Nagy, A. J. van Winkelhoff. The influence of incubation time, sample preparation and exposure to oxygen on the quality of the MALDI-TOF MS spectrum of anaerobic bacteria. Clin. Microdiol. Infect., 2014, 20: 1091-1097.
[17]	Brook I. Veillonella infections in children. J. Clin. Microbiol. 1996, 34: 1283-1285.
[18]	Reig M., N. Mir, F. Baquero. Penicillin resistance in Veillonella. Antimicrob. Agents Chemother., 1997, 41: 1210.
[19]	Nyfor S., E. Kononen, A. Bryk, R. Syrjanen, H. Jousimies-Somer. Age-related frequency of penicillin resistance of oral Veillonella. Diagn. Microbiol. Inf. Dis., 2003, 46: 279-283.
[20]	Ready D., R. Bedi, P. Mullany, M. Wilson. Penicillin and amoxicillin resistance in oral Veillonella spp. Int. J. Antimicrob. Agents, 2012, 40: 188-189.

Cite This Article

Plain Text BibTeX RIS

APA Style

Irina Ivanovna Shilnikova, Irina Aleksandrovna Kluchnikova, Inna Vasilyevna Tereshchenko, Natalia Vladimirovna Dmitrieva. (2017). Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility. American Journal of Biomedical and Life Sciences, 5(4), 82-87. https://doi.org/10.11648/j.ajbls.20170504.16

Copy | Download

ACS Style

Irina Ivanovna Shilnikova; Irina Aleksandrovna Kluchnikova; Inna Vasilyevna Tereshchenko; Natalia Vladimirovna Dmitrieva. Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility. Am. J. Biomed. Life Sci. 2017, 5(4), 82-87. doi: 10.11648/j.ajbls.20170504.16

Copy | Download

AMA Style

Irina Ivanovna Shilnikova, Irina Aleksandrovna Kluchnikova, Inna Vasilyevna Tereshchenko, Natalia Vladimirovna Dmitrieva. Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility. Am J Biomed Life Sci. 2017;5(4):82-87. doi: 10.11648/j.ajbls.20170504.16

Copy | Download

@article{10.11648/j.ajbls.20170504.16,
  author = {Irina Ivanovna Shilnikova and Irina Aleksandrovna Kluchnikova and Inna Vasilyevna Tereshchenko and Natalia Vladimirovna Dmitrieva},
  title = {Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {5},
  number = {4},
  pages = {82-87},
  doi = {10.11648/j.ajbls.20170504.16},
  url = {https://doi.org/10.11648/j.ajbls.20170504.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20170504.16},
  abstract = {We investigated the possibilities of the MALDI-TOF MS for species identification of anaerobic gram-negative cocci isolated from clinical specimens of cancer patients. A total 70 Veillonella clinical isolates and one Acidaminococcus intestini isolate were analysed by the Bruker Microflex MALDI-TOF instrument with the Biotyper 3, 0 software. All isolates were identified to the species level with a scores greater than 1.9. The most common species were V. parvula (37 strains), then followed by decreasing the frequency V. dispar (16), V. atypica (16) and V. denticariosi (1). Susceptibilities of the isolates were determined by the E-test methodology. All Veillonella isolates were susceptible to imipenem, whereas a high resistance rates were observed for penicillin G, amoxicillin/clavulanate and metronidazole. The proportion of intermediate/resistant isolates of V. parvula, V. dispar and V. atypica to penicillin (MIC ≥ 1 µg/ml) was 86%, 85% and 100%, respectively. The resistance to amoxicillin/clavulanate (MIC 16 - 32 µg/ml) was observed in about 28,6% V. parvula isolates, 23,1% V. dispar isolates and 6,7% V. atypica isolates. According to EUCAST criteria, resistance to metronidazole (MIC ≥ 8 µg/ml) of V. parvula, V. dispar and V. atypica was 88,6%, 53,8% and 40%, respectively.},
 year = {2017}
}

Copy | Download

TY - JOUR
T1 - Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility
AU - Irina Ivanovna Shilnikova
AU - Irina Aleksandrovna Kluchnikova
AU - Inna Vasilyevna Tereshchenko
AU - Natalia Vladimirovna Dmitrieva
Y1 - 2017/10/18
PY - 2017
N1 - https://doi.org/10.11648/j.ajbls.20170504.16
DO - 10.11648/j.ajbls.20170504.16
T2 - American Journal of Biomedical and Life Sciences
JF - American Journal of Biomedical and Life Sciences
JO - American Journal of Biomedical and Life Sciences
SP - 82
EP - 87
PB - Science Publishing Group
SN - 2330-880X
UR - https://doi.org/10.11648/j.ajbls.20170504.16
AB - We investigated the possibilities of the MALDI-TOF MS for species identification of anaerobic gram-negative cocci isolated from clinical specimens of cancer patients. A total 70 Veillonella clinical isolates and one Acidaminococcus intestini isolate were analysed by the Bruker Microflex MALDI-TOF instrument with the Biotyper 3, 0 software. All isolates were identified to the species level with a scores greater than 1.9. The most common species were V. parvula (37 strains), then followed by decreasing the frequency V. dispar (16), V. atypica (16) and V. denticariosi (1). Susceptibilities of the isolates were determined by the E-test methodology. All Veillonella isolates were susceptible to imipenem, whereas a high resistance rates were observed for penicillin G, amoxicillin/clavulanate and metronidazole. The proportion of intermediate/resistant isolates of V. parvula, V. dispar and V. atypica to penicillin (MIC ≥ 1 µg/ml) was 86%, 85% and 100%, respectively. The resistance to amoxicillin/clavulanate (MIC 16 - 32 µg/ml) was observed in about 28,6% V. parvula isolates, 23,1% V. dispar isolates and 6,7% V. atypica isolates. According to EUCAST criteria, resistance to metronidazole (MIC ≥ 8 µg/ml) of V. parvula, V. dispar and V. atypica was 88,6%, 53,8% and 40%, respectively.
VL - 5
IS - 4
ER -

Copy | Download

Author Information

Irina Ivanovna Shilnikova

Department of Health, N. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow, Russian Federation
Irina Aleksandrovna Kluchnikova

Department of Health, N. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow, Russian Federation
Inna Vasilyevna Tereshchenko

Department of Health, N. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow, Russian Federation
Natalia Vladimirovna Dmitrieva

Department of Health, N. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow, Russian Federation

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Irina Ivanovna Shilnikova, Irina Aleksandrovna Kluchnikova, Inna Vasilyevna Tereshchenko, Natalia Vladimirovna Dmitrieva. (2017). Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility. American Journal of Biomedical and Life Sciences, 5(4), 82-87. https://doi.org/10.11648/j.ajbls.20170504.16

Copy | Download

ACS Style

Irina Ivanovna Shilnikova; Irina Aleksandrovna Kluchnikova; Inna Vasilyevna Tereshchenko; Natalia Vladimirovna Dmitrieva. Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility. Am. J. Biomed. Life Sci. 2017, 5(4), 82-87. doi: 10.11648/j.ajbls.20170504.16

Copy | Download

AMA Style

Irina Ivanovna Shilnikova, Irina Aleksandrovna Kluchnikova, Inna Vasilyevna Tereshchenko, Natalia Vladimirovna Dmitrieva. Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility. Am J Biomed Life Sci. 2017;5(4):82-87. doi: 10.11648/j.ajbls.20170504.16

Copy | Download

@article{10.11648/j.ajbls.20170504.16,
  author = {Irina Ivanovna Shilnikova and Irina Aleksandrovna Kluchnikova and Inna Vasilyevna Tereshchenko and Natalia Vladimirovna Dmitrieva},
  title = {Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {5},
  number = {4},
  pages = {82-87},
  doi = {10.11648/j.ajbls.20170504.16},
  url = {https://doi.org/10.11648/j.ajbls.20170504.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20170504.16},
  abstract = {We investigated the possibilities of the MALDI-TOF MS for species identification of anaerobic gram-negative cocci isolated from clinical specimens of cancer patients. A total 70 Veillonella clinical isolates and one Acidaminococcus intestini isolate were analysed by the Bruker Microflex MALDI-TOF instrument with the Biotyper 3, 0 software. All isolates were identified to the species level with a scores greater than 1.9. The most common species were V. parvula (37 strains), then followed by decreasing the frequency V. dispar (16), V. atypica (16) and V. denticariosi (1). Susceptibilities of the isolates were determined by the E-test methodology. All Veillonella isolates were susceptible to imipenem, whereas a high resistance rates were observed for penicillin G, amoxicillin/clavulanate and metronidazole. The proportion of intermediate/resistant isolates of V. parvula, V. dispar and V. atypica to penicillin (MIC ≥ 1 µg/ml) was 86%, 85% and 100%, respectively. The resistance to amoxicillin/clavulanate (MIC 16 - 32 µg/ml) was observed in about 28,6% V. parvula isolates, 23,1% V. dispar isolates and 6,7% V. atypica isolates. According to EUCAST criteria, resistance to metronidazole (MIC ≥ 8 µg/ml) of V. parvula, V. dispar and V. atypica was 88,6%, 53,8% and 40%, respectively.},
 year = {2017}
}

Copy | Download