Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model

Kenji Murata; Naohiko Kanemura; Takanori Kokubun; Tsutomu Fujino; Junichi Matsumoto; Kenji Yasui; Kiyomi Takayanagi

doi:doi:10.11648/j.ajbls.20140206.14

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Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model

Kenji Murata, Naohiko Kanemura, Takanori Kokubun, Tsutomu Fujino, Junichi Matsumoto, Kenji Yasui, Kiyomi Takayanagi

Published in American Journal of Biomedical and Life Sciences (Volume 2, Issue 6)

Received: 11 December 2014 Accepted: 22 December 2014 Published: 8 January 2015

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Abstract

Rotator cuff tears are a common injury in the elderly and often impose limitations on the range of motion and cause pain. The cause of rotator cuff tearing is unknown, and degeneration of the supraspinatus tendon with age is poorly understood.In the present study, we examined collagen degeneration of the supraspinatus tendon in the rat shoulder joint. Using rats of different age (10 weeks: young, 6 months: adult, and 2 years: old), we examined the supraspinatus tendon by immunofluorescence staining (collagen types I and III), assessed the amount of collagen and mRNA expression for collagen type 1A1, 3A1 (COL1A1, COL3A1), matrix metalloproteinase 2,9 (MMP-2, -9).After immunofluorescence staining, the layer structure of tendon to bone attachment was apparent in the young. The amount of collagen decreased in the old group. In addition the mRNA expression of Col1a1 and Col3a1 was significantly reduced in the old group.On the one hand, the old groups showed higher mRNA levels of MMP-2 and MMP-9 than the young group. The ability to synthesize collagen decreases during aging and the amount of total collagen that is present in the supraspinatus tendon is decreased. These changes may contribute to tearing/breakage of the supraspinatus tendon.

Published in	American Journal of Biomedical and Life Sciences (Volume 2, Issue 6)
DOI	10.11648/j.ajbls.20140206.14
Page(s)	156-162
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Supraspinatus Tendon, Aging, Collagen

References

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APA Style

Kenji Murata, Naohiko Kanemura, Takanori Kokubun, Tsutomu Fujino, Junichi Matsumoto, et al. (2015). Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model. American Journal of Biomedical and Life Sciences, 2(6), 156-162. https://doi.org/10.11648/j.ajbls.20140206.14

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ACS Style

Kenji Murata; Naohiko Kanemura; Takanori Kokubun; Tsutomu Fujino; Junichi Matsumoto, et al. Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model. Am. J. Biomed. Life Sci. 2015, 2(6), 156-162. doi: 10.11648/j.ajbls.20140206.14

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AMA Style

Kenji Murata, Naohiko Kanemura, Takanori Kokubun, Tsutomu Fujino, Junichi Matsumoto, et al. Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model. Am J Biomed Life Sci. 2015;2(6):156-162. doi: 10.11648/j.ajbls.20140206.14

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@article{10.11648/j.ajbls.20140206.14,
  author = {Kenji Murata and Naohiko Kanemura and Takanori Kokubun and Tsutomu Fujino and Junichi Matsumoto and Kenji Yasui and Kiyomi Takayanagi},
  title = {Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {2},
  number = {6},
  pages = {156-162},
  doi = {10.11648/j.ajbls.20140206.14},
  url = {https://doi.org/10.11648/j.ajbls.20140206.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20140206.14},
  abstract = {Rotator cuff tears are a common injury in the elderly and often impose limitations on the range of motion and cause pain. The cause of rotator cuff tearing is unknown, and degeneration of the supraspinatus tendon with age is poorly understood.In the present study, we examined collagen degeneration of the supraspinatus tendon in the rat shoulder joint. Using rats of different age (10 weeks: young, 6 months: adult, and 2 years: old), we examined the supraspinatus tendon by immunofluorescence staining (collagen types I and III), assessed the amount of collagen and mRNA expression for collagen type 1A1, 3A1 (COL1A1, COL3A1), matrix metalloproteinase 2,9 (MMP-2, -9).After immunofluorescence staining, the layer structure of tendon to bone attachment was apparent in the young. The amount of collagen decreased in the old group. In addition the mRNA expression of Col1a1 and Col3a1 was significantly reduced in the old group.On the one hand, the old groups showed higher mRNA levels of MMP-2 and MMP-9 than the young group. The ability to synthesize collagen decreases during aging and the amount of total collagen that is present in the supraspinatus tendon is decreased. These changes may contribute to tearing/breakage of the supraspinatus tendon.},
 year = {2015}
}

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TY  - JOUR
T1  - Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model
AU  - Kenji Murata
AU  - Naohiko Kanemura
AU  - Takanori Kokubun
AU  - Tsutomu Fujino
AU  - Junichi Matsumoto
AU  - Kenji Yasui
AU  - Kiyomi Takayanagi
Y1  - 2015/01/08
PY  - 2015
N1  - https://doi.org/10.11648/j.ajbls.20140206.14
DO  - 10.11648/j.ajbls.20140206.14
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 156
EP  - 162
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20140206.14
AB  - Rotator cuff tears are a common injury in the elderly and often impose limitations on the range of motion and cause pain. The cause of rotator cuff tearing is unknown, and degeneration of the supraspinatus tendon with age is poorly understood.In the present study, we examined collagen degeneration of the supraspinatus tendon in the rat shoulder joint. Using rats of different age (10 weeks: young, 6 months: adult, and 2 years: old), we examined the supraspinatus tendon by immunofluorescence staining (collagen types I and III), assessed the amount of collagen and mRNA expression for collagen type 1A1, 3A1 (COL1A1, COL3A1), matrix metalloproteinase 2,9 (MMP-2, -9).After immunofluorescence staining, the layer structure of tendon to bone attachment was apparent in the young. The amount of collagen decreased in the old group. In addition the mRNA expression of Col1a1 and Col3a1 was significantly reduced in the old group.On the one hand, the old groups showed higher mRNA levels of MMP-2 and MMP-9 than the young group. The ability to synthesize collagen decreases during aging and the amount of total collagen that is present in the supraspinatus tendon is decreased. These changes may contribute to tearing/breakage of the supraspinatus tendon.
VL  - 2
IS  - 6
ER  -

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Author Information

Kenji Murata

Department of Physical therapy, School of health and Social Services, Saitama Prefectural University, Saitama, Japan
Naohiko Kanemura

Department of Physical therapy, School of health and Social Services, Saitama Prefectural University, Saitama, Japan
Takanori Kokubun

Department of Physical therapy, School of health and Social Services, Saitama Prefectural University, Saitama, Japan
Tsutomu Fujino

Department of Rehabilitation, Urawa Orthopedics Clinic, Saitama, Japan
Junichi Matsumoto

Department of Rehabilitation, Kasukabe Chuo general Hospital, Saitama, Japan
Kenji Yasui

Department of Orthopedics, Tokyo Women’s Medical University, Tokyo, Japan
Kiyomi Takayanagi

Department of Physical therapy, School of health and Social Services, Saitama Prefectural University, Saitama, Japan

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APA Style

Kenji Murata, Naohiko Kanemura, Takanori Kokubun, Tsutomu Fujino, Junichi Matsumoto, et al. (2015). Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model. American Journal of Biomedical and Life Sciences, 2(6), 156-162. https://doi.org/10.11648/j.ajbls.20140206.14

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ACS Style

Kenji Murata; Naohiko Kanemura; Takanori Kokubun; Tsutomu Fujino; Junichi Matsumoto, et al. Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model. Am. J. Biomed. Life Sci. 2015, 2(6), 156-162. doi: 10.11648/j.ajbls.20140206.14

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AMA Style

Kenji Murata, Naohiko Kanemura, Takanori Kokubun, Tsutomu Fujino, Junichi Matsumoto, et al. Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model. Am J Biomed Life Sci. 2015;2(6):156-162. doi: 10.11648/j.ajbls.20140206.14

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@article{10.11648/j.ajbls.20140206.14,
  author = {Kenji Murata and Naohiko Kanemura and Takanori Kokubun and Tsutomu Fujino and Junichi Matsumoto and Kenji Yasui and Kiyomi Takayanagi},
  title = {Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {2},
  number = {6},
  pages = {156-162},
  doi = {10.11648/j.ajbls.20140206.14},
  url = {https://doi.org/10.11648/j.ajbls.20140206.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20140206.14},
  abstract = {Rotator cuff tears are a common injury in the elderly and often impose limitations on the range of motion and cause pain. The cause of rotator cuff tearing is unknown, and degeneration of the supraspinatus tendon with age is poorly understood.In the present study, we examined collagen degeneration of the supraspinatus tendon in the rat shoulder joint. Using rats of different age (10 weeks: young, 6 months: adult, and 2 years: old), we examined the supraspinatus tendon by immunofluorescence staining (collagen types I and III), assessed the amount of collagen and mRNA expression for collagen type 1A1, 3A1 (COL1A1, COL3A1), matrix metalloproteinase 2,9 (MMP-2, -9).After immunofluorescence staining, the layer structure of tendon to bone attachment was apparent in the young. The amount of collagen decreased in the old group. In addition the mRNA expression of Col1a1 and Col3a1 was significantly reduced in the old group.On the one hand, the old groups showed higher mRNA levels of MMP-2 and MMP-9 than the young group. The ability to synthesize collagen decreases during aging and the amount of total collagen that is present in the supraspinatus tendon is decreased. These changes may contribute to tearing/breakage of the supraspinatus tendon.},
 year = {2015}
}

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TY  - JOUR
T1  - Age-Related Changes in Collagen Degeneration of the Rotator Cuff in an Animal Model
AU  - Kenji Murata
AU  - Naohiko Kanemura
AU  - Takanori Kokubun
AU  - Tsutomu Fujino
AU  - Junichi Matsumoto
AU  - Kenji Yasui
AU  - Kiyomi Takayanagi
Y1  - 2015/01/08
PY  - 2015
N1  - https://doi.org/10.11648/j.ajbls.20140206.14
DO  - 10.11648/j.ajbls.20140206.14
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 156
EP  - 162
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20140206.14
AB  - Rotator cuff tears are a common injury in the elderly and often impose limitations on the range of motion and cause pain. The cause of rotator cuff tearing is unknown, and degeneration of the supraspinatus tendon with age is poorly understood.In the present study, we examined collagen degeneration of the supraspinatus tendon in the rat shoulder joint. Using rats of different age (10 weeks: young, 6 months: adult, and 2 years: old), we examined the supraspinatus tendon by immunofluorescence staining (collagen types I and III), assessed the amount of collagen and mRNA expression for collagen type 1A1, 3A1 (COL1A1, COL3A1), matrix metalloproteinase 2,9 (MMP-2, -9).After immunofluorescence staining, the layer structure of tendon to bone attachment was apparent in the young. The amount of collagen decreased in the old group. In addition the mRNA expression of Col1a1 and Col3a1 was significantly reduced in the old group.On the one hand, the old groups showed higher mRNA levels of MMP-2 and MMP-9 than the young group. The ability to synthesize collagen decreases during aging and the amount of total collagen that is present in the supraspinatus tendon is decreased. These changes may contribute to tearing/breakage of the supraspinatus tendon.
VL  - 2
IS  - 6
ER  -

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