Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages

Rongfa Chen; Xing Huang; Lihang Qiu; Yegeng Fan; Ronghua Zhang; Jinlan Xie; Jianming Wu; Yangrui Li

doi:doi:10.11648/j.ajpb.20180302.12

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Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages

Rongfa Chen, Xing Huang, Lihang Qiu, Yegeng Fan, Ronghua Zhang, Jinlan Xie, Jianming Wu, Yangrui Li

Published in American Journal of Plant Biology (Volume 3, Issue 2)

Received: 22 August 2018 Accepted: 19 September 2018 Published: 10 October 2018

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Abstract

Sugarcane is the most important sugar crop in China, which mainly focuses on the upper stem of the harvested land. The proper regulation of the proportion of internode elongation is the key to determine the yield and sugar. Therefore, it is of great significance to study the mechanism of the dynamic change of elongation between cane joints in order to improve the yield of sugar cane and sucrose. To investigate the biochemical mechanism of stem elongation in sugarcane, stem samples were collected at the pre-elongation stage (9-10 leaves) (Ls1), early elongation stage (12-13 leaves) (Ls2) and rapid elongation stage (15-16 leaves) (Ls3). The change trends in the activities of NAD kinase (NADK), calcium-dependent protein kinase (CDPKs), α-mannosidase, α-galactosidase, β-glucosidase, cellulase, xyloglucan endo-transglycosylase/hydrolase (XTH) and catalase (CAT) were completely consistent, showing rapid elongation stage > early elongation stage > pre-elongation stage, while the activities of β-glucosidase, peroxidase (POD) and α-glucosidase were in opposite, and the activities of calmodulin and β-mannosidase showed the same single-peak trend, and the peak was at early elongation stage. The enzyme activities of β-galactosidase and pectinase did not show significant difference at different stages. The results indicate that the elongation of internodes was closely related to the complex physiological metabolism of sugarcane, and the key enzymes play roles at different time but β-galactosidase and pectinase have little effect on internodes elongation in sugarcane.

Published in	American Journal of Plant Biology (Volume 3, Issue 2)
DOI	10.11648/j.ajpb.20180302.12
Page(s)	21-28
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Sugarcane, Stem Elongation, Enzyme Activity, Metabolism

References

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APA Style

Rongfa Chen, Xing Huang, Lihang Qiu, Yegeng Fan, Ronghua Zhang, et al. (2018). Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages. American Journal of Plant Biology, 3(2), 21-28. https://doi.org/10.11648/j.ajpb.20180302.12

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ACS Style

Rongfa Chen; Xing Huang; Lihang Qiu; Yegeng Fan; Ronghua Zhang, et al. Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages. Am. J. Plant Biol. 2018, 3(2), 21-28. doi: 10.11648/j.ajpb.20180302.12

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AMA Style

Rongfa Chen, Xing Huang, Lihang Qiu, Yegeng Fan, Ronghua Zhang, et al. Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages. Am J Plant Biol. 2018;3(2):21-28. doi: 10.11648/j.ajpb.20180302.12

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@article{10.11648/j.ajpb.20180302.12,
  author = {Rongfa Chen and Xing Huang and Lihang Qiu and Yegeng Fan and Ronghua Zhang and Jinlan Xie and Jianming Wu and Yangrui Li},
  title = {Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages},
  journal = {American Journal of Plant Biology},
  volume = {3},
  number = {2},
  pages = {21-28},
  doi = {10.11648/j.ajpb.20180302.12},
  url = {https://doi.org/10.11648/j.ajpb.20180302.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.20180302.12},
  abstract = {Sugarcane is the most important sugar crop in China, which mainly focuses on the upper stem of the harvested land. The proper regulation of the proportion of internode elongation is the key to determine the yield and sugar. Therefore, it is of great significance to study the mechanism of the dynamic change of elongation between cane joints in order to improve the yield of sugar cane and sucrose. To investigate the biochemical mechanism of stem elongation in sugarcane, stem samples were collected at the pre-elongation stage (9-10 leaves) (Ls1), early elongation stage (12-13 leaves) (Ls2) and rapid elongation stage (15-16 leaves) (Ls3). The change trends in the activities of NAD kinase (NADK), calcium-dependent protein kinase (CDPKs), α-mannosidase, α-galactosidase, β-glucosidase, cellulase, xyloglucan endo-transglycosylase/hydrolase (XTH) and catalase (CAT) were completely consistent, showing rapid elongation stage > early elongation stage > pre-elongation stage, while the activities of β-glucosidase, peroxidase (POD) and α-glucosidase were in opposite, and the activities of calmodulin and β-mannosidase showed the same single-peak trend, and the peak was at early elongation stage. The enzyme activities of β-galactosidase and pectinase did not show significant difference at different stages. The results indicate that the elongation of internodes was closely related to the complex physiological metabolism of sugarcane, and the key enzymes play roles at different time but β-galactosidase and pectinase have little effect on internodes elongation in sugarcane.},
 year = {2018}
}

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TY  - JOUR
T1  - Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages
AU  - Rongfa Chen
AU  - Xing Huang
AU  - Lihang Qiu
AU  - Yegeng Fan
AU  - Ronghua Zhang
AU  - Jinlan Xie
AU  - Jianming Wu
AU  - Yangrui Li
Y1  - 2018/10/10
PY  - 2018
N1  - https://doi.org/10.11648/j.ajpb.20180302.12
DO  - 10.11648/j.ajpb.20180302.12
T2  - American Journal of Plant Biology
JF  - American Journal of Plant Biology
JO  - American Journal of Plant Biology
SP  - 21
EP  - 28
PB  - Science Publishing Group
SN  - 2578-8337
UR  - https://doi.org/10.11648/j.ajpb.20180302.12
AB  - Sugarcane is the most important sugar crop in China, which mainly focuses on the upper stem of the harvested land. The proper regulation of the proportion of internode elongation is the key to determine the yield and sugar. Therefore, it is of great significance to study the mechanism of the dynamic change of elongation between cane joints in order to improve the yield of sugar cane and sucrose. To investigate the biochemical mechanism of stem elongation in sugarcane, stem samples were collected at the pre-elongation stage (9-10 leaves) (Ls1), early elongation stage (12-13 leaves) (Ls2) and rapid elongation stage (15-16 leaves) (Ls3). The change trends in the activities of NAD kinase (NADK), calcium-dependent protein kinase (CDPKs), α-mannosidase, α-galactosidase, β-glucosidase, cellulase, xyloglucan endo-transglycosylase/hydrolase (XTH) and catalase (CAT) were completely consistent, showing rapid elongation stage > early elongation stage > pre-elongation stage, while the activities of β-glucosidase, peroxidase (POD) and α-glucosidase were in opposite, and the activities of calmodulin and β-mannosidase showed the same single-peak trend, and the peak was at early elongation stage. The enzyme activities of β-galactosidase and pectinase did not show significant difference at different stages. The results indicate that the elongation of internodes was closely related to the complex physiological metabolism of sugarcane, and the key enzymes play roles at different time but β-galactosidase and pectinase have little effect on internodes elongation in sugarcane.
VL  - 3
IS  - 2
ER  -

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Author Information

Rongfa Chen

Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China
Xing Huang

Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China
Lihang Qiu

Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China
Yegeng Fan

Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China
Ronghua Zhang

Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China
Jinlan Xie

Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China
Jianming Wu

Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China
Yangrui Li

Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China; Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Nanning, China

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Plain Text BibTeX RIS

APA Style

Rongfa Chen, Xing Huang, Lihang Qiu, Yegeng Fan, Ronghua Zhang, et al. (2018). Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages. American Journal of Plant Biology, 3(2), 21-28. https://doi.org/10.11648/j.ajpb.20180302.12

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ACS Style

Rongfa Chen; Xing Huang; Lihang Qiu; Yegeng Fan; Ronghua Zhang, et al. Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages. Am. J. Plant Biol. 2018, 3(2), 21-28. doi: 10.11648/j.ajpb.20180302.12

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AMA Style

Rongfa Chen, Xing Huang, Lihang Qiu, Yegeng Fan, Ronghua Zhang, et al. Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages. Am J Plant Biol. 2018;3(2):21-28. doi: 10.11648/j.ajpb.20180302.12

Copy | Download

@article{10.11648/j.ajpb.20180302.12,
  author = {Rongfa Chen and Xing Huang and Lihang Qiu and Yegeng Fan and Ronghua Zhang and Jinlan Xie and Jianming Wu and Yangrui Li},
  title = {Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages},
  journal = {American Journal of Plant Biology},
  volume = {3},
  number = {2},
  pages = {21-28},
  doi = {10.11648/j.ajpb.20180302.12},
  url = {https://doi.org/10.11648/j.ajpb.20180302.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.20180302.12},
  abstract = {Sugarcane is the most important sugar crop in China, which mainly focuses on the upper stem of the harvested land. The proper regulation of the proportion of internode elongation is the key to determine the yield and sugar. Therefore, it is of great significance to study the mechanism of the dynamic change of elongation between cane joints in order to improve the yield of sugar cane and sucrose. To investigate the biochemical mechanism of stem elongation in sugarcane, stem samples were collected at the pre-elongation stage (9-10 leaves) (Ls1), early elongation stage (12-13 leaves) (Ls2) and rapid elongation stage (15-16 leaves) (Ls3). The change trends in the activities of NAD kinase (NADK), calcium-dependent protein kinase (CDPKs), α-mannosidase, α-galactosidase, β-glucosidase, cellulase, xyloglucan endo-transglycosylase/hydrolase (XTH) and catalase (CAT) were completely consistent, showing rapid elongation stage > early elongation stage > pre-elongation stage, while the activities of β-glucosidase, peroxidase (POD) and α-glucosidase were in opposite, and the activities of calmodulin and β-mannosidase showed the same single-peak trend, and the peak was at early elongation stage. The enzyme activities of β-galactosidase and pectinase did not show significant difference at different stages. The results indicate that the elongation of internodes was closely related to the complex physiological metabolism of sugarcane, and the key enzymes play roles at different time but β-galactosidase and pectinase have little effect on internodes elongation in sugarcane.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages
AU  - Rongfa Chen
AU  - Xing Huang
AU  - Lihang Qiu
AU  - Yegeng Fan
AU  - Ronghua Zhang
AU  - Jinlan Xie
AU  - Jianming Wu
AU  - Yangrui Li
Y1  - 2018/10/10
PY  - 2018
N1  - https://doi.org/10.11648/j.ajpb.20180302.12
DO  - 10.11648/j.ajpb.20180302.12
T2  - American Journal of Plant Biology
JF  - American Journal of Plant Biology
JO  - American Journal of Plant Biology
SP  - 21
EP  - 28
PB  - Science Publishing Group
SN  - 2578-8337
UR  - https://doi.org/10.11648/j.ajpb.20180302.12
AB  - Sugarcane is the most important sugar crop in China, which mainly focuses on the upper stem of the harvested land. The proper regulation of the proportion of internode elongation is the key to determine the yield and sugar. Therefore, it is of great significance to study the mechanism of the dynamic change of elongation between cane joints in order to improve the yield of sugar cane and sucrose. To investigate the biochemical mechanism of stem elongation in sugarcane, stem samples were collected at the pre-elongation stage (9-10 leaves) (Ls1), early elongation stage (12-13 leaves) (Ls2) and rapid elongation stage (15-16 leaves) (Ls3). The change trends in the activities of NAD kinase (NADK), calcium-dependent protein kinase (CDPKs), α-mannosidase, α-galactosidase, β-glucosidase, cellulase, xyloglucan endo-transglycosylase/hydrolase (XTH) and catalase (CAT) were completely consistent, showing rapid elongation stage > early elongation stage > pre-elongation stage, while the activities of β-glucosidase, peroxidase (POD) and α-glucosidase were in opposite, and the activities of calmodulin and β-mannosidase showed the same single-peak trend, and the peak was at early elongation stage. The enzyme activities of β-galactosidase and pectinase did not show significant difference at different stages. The results indicate that the elongation of internodes was closely related to the complex physiological metabolism of sugarcane, and the key enzymes play roles at different time but β-galactosidase and pectinase have little effect on internodes elongation in sugarcane.
VL  - 3
IS  - 2
ER  -

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