Bioprocess Engineering

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Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration

Received: 21 February 2018    Accepted: 11 March 2018    Published: 08 April 2018
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Abstract

Estimation of the energy exchange parameters during partial brain ischemia offers an opportunity for finding new ways of correcting the above pathology. The aim of the study was to investigate the changes in mitochondrial respiration in the homogenates of rats with brain cerebral ischemia of various duration. The experiment was carried out on 19 white female rats in whom the right common carotid artery was ligated for 1 hour or 3 days with subsequent decapitation and brain removal. The rate of mitochondrial respiration in mitochondrial suspension of brain homogenates was assessed by means of the polarographic method using succinate or a mixture of L-malate/L-glutamate and adenosine diphosphate as respiratory substrates. Ligation of the right common carotid artery of various duration resulted in reduced mitochondrial respiration rate which was more pronounced after 1 hour of partial brain ischemia as compared to 3 hours. Modeling of partial cerebral ischemia in rats by ligation of the right common carotid artery considerably inhibits respiratory activity of the brain mitochondria leading to the dissociation of oxidation and phosphorylation in the mitochondria and inducing impairment of neuronal bioenergetics in cerebral tissue due to the disturbance of the supply of oxygen by blood. Understanding of pathophysiological processes in mitochondria during partial brain ischemia will help to develop new methods of pathogenetic correction of changes in cell structures of the brain tissue. This approach offers new possibilities for the pathogenetic therapy.

DOI 10.11648/j.be.20180201.12
Published in Bioprocess Engineering (Volume 2, Issue 1, March 2018)
Page(s) 6-9
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Rats, Brain, Partial Ischemia, Mitochondrial Respiration

References
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[2] Bova AA, Silinovich MV (2015) Ischemic stroke: the strategy of patient management (compliance of recommendations and their real clinical application) Military medicine. 4 (37): 30–35.
[3] Directive of the European Parliament and Council (2012) 2010/63 / EU of 22.09.2010 on the protection of animals used for scientific purposes. St. Petersburg: 50.
[4] Gusev EI, Martynov MYu, Kamchatnov PR (2013) Ischemic stroke. Current state of the problem. Doktor. ru. Moscow, 5 (83): 2–7.
[5] Hossman KA (1998) Experimental models for the investigation of brain ischemia Cardiovascular Resarch. 39: 106–120.
[6] Lai JCK, Clark JB (1974) Preparation of synaptic and nonsynaptic mitochondria from mammalian brain Methods in Enzymology. 60: 51–64.
[7] Maksimovich NE (2003) The concept of the nitroxidergic system of the brain. The role of neuronal sources Journal of the Grodno State Medical University. 4: 7–11.
[8] Maksimovich NE (2004) Peculiarities of formation of the level of azoate oxide in rat blood plasma in ischemic and reperfusion injuries of the brain Regional circulation and microcirculation. 3 (11): 55–60.
[9] Maksimovich NE (2010) Modern ideas about the mechanisms of development of ischemic brain damage and ways of correction of this pathology Actual theoretical and applied aspects of pathophysiology: materials. Republican scientific-practical conference with international participation May 14, 2010–Grodno: 149–157.
[10] Rukan TA, Maksimovich NE, Zimatkin SM (2012) Morphofunctional changes in the neurons of the frontal cortex of rat brain under conditions of its ischemia-reperfusion Journal of the Grodno State Medical University. 4: 35–38.
[11] Solodkov AP, Chirkin AA (2010) Modern problems of biochemistry: textbook Vitebsk: "VSU named after P. M. Masherov ": 384.
[12] Starodubtseva OS, Begicheva S. V. (2012) Analysis of the incidence of stroke using information technology. Fundamental research.; 8 (part 2): 424–27.
[13] Suslina ZA, Piradov MA, Domashenko MA (2014) Stroke: the review of the problem (15 years later)]. Journal of Neurology and Psychiatry named after S. S. Korsakova 11: 5–13.
[14] Usova N. N., Lemeshkov L. A., Malkov A. B., Ivantsov OA, Galynovskaya N. V., Latysheva V. Ya. (2015) Diseases of the nervous system in geriatrics: A manual for physicians–Gomel–99.
[15] WHO report (2013) Information Bulletin 310: www.who.int.
Author Information
  • Department of Pathophysiology of the Name of D. A. Maslakov, Grodno State Medical University, Grodno, Belarus; Department of Obstetrics and Gynecology, Grodno State Medical University, Grodno, Belarus

  • Department of Pathophysiology of the Name of D. A. Maslakov, Grodno State Medical University, Grodno, Belarus; Department of Obstetrics and Gynecology, Grodno State Medical University, Grodno, Belarus

  • Department of Pathophysiology of the Name of D. A. Maslakov, Grodno State Medical University, Grodno, Belarus; Department of Obstetrics and Gynecology, Grodno State Medical University, Grodno, Belarus

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    Nataliya Yaugenievna Maksimovich, Tatsiana Sergeevna Milosh, Iosif Karlovich Dremza. (2018). Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration. Bioprocess Engineering, 2(1), 6-9. https://doi.org/10.11648/j.be.20180201.12

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    ACS Style

    Nataliya Yaugenievna Maksimovich; Tatsiana Sergeevna Milosh; Iosif Karlovich Dremza. Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration. Bioprocess Eng. 2018, 2(1), 6-9. doi: 10.11648/j.be.20180201.12

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    AMA Style

    Nataliya Yaugenievna Maksimovich, Tatsiana Sergeevna Milosh, Iosif Karlovich Dremza. Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration. Bioprocess Eng. 2018;2(1):6-9. doi: 10.11648/j.be.20180201.12

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  • @article{10.11648/j.be.20180201.12,
      author = {Nataliya Yaugenievna Maksimovich and Tatsiana Sergeevna Milosh and Iosif Karlovich Dremza},
      title = {Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration},
      journal = {Bioprocess Engineering},
      volume = {2},
      number = {1},
      pages = {6-9},
      doi = {10.11648/j.be.20180201.12},
      url = {https://doi.org/10.11648/j.be.20180201.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.be.20180201.12},
      abstract = {Estimation of the energy exchange parameters during partial brain ischemia offers an opportunity for finding new ways of correcting the above pathology. The aim of the study was to investigate the changes in mitochondrial respiration in the homogenates of rats with brain cerebral ischemia of various duration. The experiment was carried out on 19 white female rats in whom the right common carotid artery was ligated for 1 hour or 3 days with subsequent decapitation and brain removal. The rate of mitochondrial respiration in mitochondrial suspension of brain homogenates was assessed by means of the polarographic method using succinate or a mixture of L-malate/L-glutamate and adenosine diphosphate as respiratory substrates. Ligation of the right common carotid artery of various duration resulted in reduced mitochondrial respiration rate which was more pronounced after 1 hour of partial brain ischemia as compared to 3 hours. Modeling of partial cerebral ischemia in rats by ligation of the right common carotid artery considerably inhibits respiratory activity of the brain mitochondria leading to the dissociation of oxidation and phosphorylation in the mitochondria and inducing impairment of neuronal bioenergetics in cerebral tissue due to the disturbance of the supply of oxygen by blood. Understanding of pathophysiological processes in mitochondria during partial brain ischemia will help to develop new methods of pathogenetic correction of changes in cell structures of the brain tissue. This approach offers new possibilities for the pathogenetic therapy.},
     year = {2018}
    }
    

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    T1  - Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration
    AU  - Nataliya Yaugenievna Maksimovich
    AU  - Tatsiana Sergeevna Milosh
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    Y1  - 2018/04/08
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    DO  - 10.11648/j.be.20180201.12
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    JF  - Bioprocess Engineering
    JO  - Bioprocess Engineering
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    PB  - Science Publishing Group
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    AB  - Estimation of the energy exchange parameters during partial brain ischemia offers an opportunity for finding new ways of correcting the above pathology. The aim of the study was to investigate the changes in mitochondrial respiration in the homogenates of rats with brain cerebral ischemia of various duration. The experiment was carried out on 19 white female rats in whom the right common carotid artery was ligated for 1 hour or 3 days with subsequent decapitation and brain removal. The rate of mitochondrial respiration in mitochondrial suspension of brain homogenates was assessed by means of the polarographic method using succinate or a mixture of L-malate/L-glutamate and adenosine diphosphate as respiratory substrates. Ligation of the right common carotid artery of various duration resulted in reduced mitochondrial respiration rate which was more pronounced after 1 hour of partial brain ischemia as compared to 3 hours. Modeling of partial cerebral ischemia in rats by ligation of the right common carotid artery considerably inhibits respiratory activity of the brain mitochondria leading to the dissociation of oxidation and phosphorylation in the mitochondria and inducing impairment of neuronal bioenergetics in cerebral tissue due to the disturbance of the supply of oxygen by blood. Understanding of pathophysiological processes in mitochondria during partial brain ischemia will help to develop new methods of pathogenetic correction of changes in cell structures of the brain tissue. This approach offers new possibilities for the pathogenetic therapy.
    VL  - 2
    IS  - 1
    ER  - 

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