Plasmid Profile Analysis and Curing of Multidrug-Resistant Bacteria Isolated from Hospital Environmental Surfaces in Akure Metropolis, Ondo State, Nigeria

Anthony Kayode Onifade; Ogedegbe Gloria Palmer

doi:doi:10.11648/j.ajist.20180201.13

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Plasmid Profile Analysis and Curing of Multidrug-Resistant Bacteria Isolated from Hospital Environmental Surfaces in Akure Metropolis, Ondo State, Nigeria

Anthony Kayode Onifade, Ogedegbe Gloria Palmer

Published in American Journal of Information Science and Technology (Volume 2, Issue 1)

Received: 19 April 2018 Accepted: 2 May 2018 Published: 24 May 2018

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Abstract

Plasmid profile analysis and curing of multidrug-resistant bacteria isolated from hospital environment in Akure metropolis, Ondo State Nigeria were investigated. Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Bacillus cereus were the bacterial isolated in the course of the research. The result revealed that Staphylococcus aureus and Pseudomonas aeruginosa showed multiple antibiotics resistance. Staphylococcus aureus and Pseudomonas aeruginosa are rapidly increasing as multidrug resistant strains worldwide. The results of antibiotics sensitivity test on the microorganisms subjected to plasmid analysis indicated that they were more susceptible to antibiotics after the plasmid curing of the microorganisms. The results from this investigative study have shown that resistance in S. aureus and P. aeruginosa are plasmid based as a result of its loss after curing.

Published in	American Journal of Information Science and Technology (Volume 2, Issue 1)
DOI	10.11648/j.ajist.20180201.13
Page(s)	18-23
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Plasmid, Hospital, Environment, Multiple, Antibiotics

References

[1]	Safdar, N., Crnich, C. J. and Maki, D. G. (2001). Nosocomial infections in the intensive care unit associated with invasive medical devices. Current Infectious Disease Reports, 1: 487–495.
[2]	Burke, J. P. (2003). Infection Control—A Problem for Patient Safety. New England Journal of Medicine, 348: 651–656
[3]	Krzowska-Firych, J., Kozlowska, A., Sukhadia, T. and Al-Mosawi, L. K. (2014). Hospital-Acquired infection cause by antibiotic resistance. Postepy Nauk Medycynych, t. XXVII, nr 11: 783-786
[4]	Sehulster, L. M., Chinn, R. Y. W., Arduino, M. J., Carpenter, J., Donlan, R. and Ashford, D. (2003). Guidelines for Environmental Infection Control in Health-Care Facilities. Recommendations from Centers for Disease Control and Prevention (CDC) and the Healthcare Infection Control Practices Advisory Committee (HICPAC). Chicago: American Society for Healthcare Engineering/American Hospital Association. http://www.cdc.gov/hicpac/pdf/guidelines/eic_in_HCF_03.pdf. Accessed 13 January 2012.
[5]	ASM. (2009). Antibiotic Resistance: An Ecological Perspective on an Old Problem. Report from the American Academy of Microbiology. American Society for Microbiology, Washington, DC.
[6]	Barrie, D., Hoffman, P. N., Wilson, J. A. and Kramer, J. M. (1994). Contamination of hospital linen by Bacillus cereus. Epidemiology and Infection, 113(2): 297-306.
[7]	Hardy, K. J., Oppenheim, B. A., Gossain, S., Gao, F. and Hawkey, P. M. (2006). A Study of the Relationship between Environmental Contamination with Methicillin-Resistant Staphylococcus aureus (MRSA) and Patients’ Acquisition of MRSA. Infection Control and Hospital Epidemiology, 27: 127-132.
[8]	Sexton, T., Clark, P., O’Neill, E., Dillane, T. and Humphreys, H. (2006). Environmental Reservoirs of Methicillin-Resistant Staphylococcus aureus in Isolation Rooms: Correlation with Patient Isolates and Implications for Hospital Hygiene. Journal of Hospital Infection, 62: 187-194.
[9]	Hayden, M. K., Blom, D. W., Lyle, E. A., Moore, C. G. and Weistein, R. A. (2008). Risk of Hand or Glove Contamination after Contact with Vancomycin-Resistant Enterococcus or the Colonized Patients’ Environment. Infection Control and Hospital Epidemiology, 29: 149-154.
[10]	Wise, R., Hart, T., Cars, O., Streulens, M., Helmuth, R., Huovinen, P., Sprenger, M. (1998). Antimicrobial resistance is a major threat to public health. Bristish Medical Journal, 317: 609-610.
[11]	Mazel, D. and Davies, J. (1999). Antibiotic resistancein microbes. Cellular and Molecular Life Sciences, 56: 746-752.
[12]	Islam, Q. (2011). Antimicrobial Resistance: A Man Made Crisis. J. Bangladesh Coll. Physicians. Surgeons, 29: 120-125.
[13]	Berger-Bachi, B. (2002). Resistance mechanisms of gram-positive bacteria. International Journal of Medical Microbiology, 292: 27-35.
[14]	Moura, A., Pereira, C., Henriques, I. and Correia, A. (2011). Novel gene cassettes and integrons in antibiotic-resistant bacteria from urban wastewaters. Research in Microbiology (early on-line).
[15]	Bakkali, M. E., Hmid, K., Kari, K. E., Zouhdi, M., Mzibri, M. E. and Lalaoui, A. (2015). Characterization of bacterial strains and their resistance status in hospital environment. Journal of Tropical Disease, 4: 180.
[16]	Olutiola, P. O., Famurewa, O. and Senntag, H. G. (2000). An introduction to General microbiology, Hygiene institute Der UniversitalHeideberg. Federal Republic of Germany. Pp 267.
[17]	Cheesbrough, M. (2010). District Laboratory Practice in Tropical Countries, 2ndedition, Cambridge University press, Cambridge University Press, New York, pp. 70 -71.
[18]	Barnett, H. L. and Hunter, B. B. (1998). Illustrated genera of imperfect fungi. 4th edition. St. Paul, Minn: APS Press. pp. 2, 70, 92-94.
[19]	Bauer, A. W., Kirby, W. M., Sherris, J. C. and Turck, M. (1996). Antibiotic susceptibility testing by a standardized single disk method. American journal of Clinical Pathology, 459(4): 493-6.
[20]	Molina-Aja, A., Garcia-Gasca, A., Abreu-Grobios, A., Bolan-Mejia, C., Roque, A. and Gomez-Grill, B. (2002). Plasmid profiling and antibiotic resistance of Vibrio strains isolated from cultural panied shrimp. FEMS Microbiology Letter, 213: 7- 12.
[21]	Akinjogunla, O. J. and Enabulele, I. O. (2010). Virulence Factors, Plasmid Profiling and Curing analysis of Multi- drug Resistant Staphylococcus aureus and Coagulase negative Staphylococcus spp. isolated from Patients with Acute Otitis Media. Journal of American Science, 6(11):1022-1033
[22]	Awosika, S. A., Olajubu, F. A., and Amusa, N. A. (2012). Microbiological assessment of indoor air of a teaching hospital in Nigeria. Asian Pacific Journal of Tropical Biomedicine, 2(6): 465-458
[23]	Caggiano, G., Napoli, C., Coretti, C., Lovero, G., Scarafile, G., Gigilio, O. D. and Montagna, T. (2014). Mold contamination in the controlled hospital environment: A 3 years surveillance in southern Italy. BMC Infectious Diseases, 14: 595
[24]	Hauser, A. R. and Sriram, P. (2005). Severe Pseudomonas aeruginosa infections. Tackling the conundrum of drug resistance. Postgraduate Medicine, 117: 41–48.
[25]	Maltezou, H. C. and Giamarellou, H. (2006). Community-acquired methicillin-resistant Staphylococcus aureus infections. International Journal of Antimicrobial Agents, 27: 87–96.
[26]	Seza, A, and Fatma, O. (2012). Antimicrobial resistant of Staphylococcus aureus isolated from human and food against linezolid, quinuprsiti-dalforpristin, quinolones and imipenem. African journal of microbiology research, 6(11): 2616–2621.
[27]	Carmeli, Y., Troillet, N., Eliopoulos, G. and Samore. M. H. (1999). Emergence of antibiotic-resistant Pseudomonas aeruginosa: comparison of risks associated with different antipseudomonal agents. Antimicrobial Agents and Chemotherapy, 43: 1379-1382.
[28]	Agbagwa, O. E., Otokunefor, T. V. and Frank-Peterside, N. (2012). Plasmid Profile Analysis of Bacteria Isolated From Selected Honey Samples in Nigeria. Journal of Animal Sciences Advances, 2(3.2): 338-343
[29]	Onifade, A. K. and Oladoja, M. A. (2015). Molecular epidemiology of Shiga Toxin producing Escherichia coli isolated from school children in Ondo State, Nigeria. British Microbiology Research Journal, 6(6): 340–347.

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Anthony Kayode Onifade, Ogedegbe Gloria Palmer. (2018). Plasmid Profile Analysis and Curing of Multidrug-Resistant Bacteria Isolated from Hospital Environmental Surfaces in Akure Metropolis, Ondo State, Nigeria. American Journal of Information Science and Technology, 2(1), 18-23. https://doi.org/10.11648/j.ajist.20180201.13

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ACS Style

Anthony Kayode Onifade; Ogedegbe Gloria Palmer. Plasmid Profile Analysis and Curing of Multidrug-Resistant Bacteria Isolated from Hospital Environmental Surfaces in Akure Metropolis, Ondo State, Nigeria. Am. J. Inf. Sci. Technol. 2018, 2(1), 18-23. doi: 10.11648/j.ajist.20180201.13

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AMA Style

Anthony Kayode Onifade, Ogedegbe Gloria Palmer. Plasmid Profile Analysis and Curing of Multidrug-Resistant Bacteria Isolated from Hospital Environmental Surfaces in Akure Metropolis, Ondo State, Nigeria. Am J Inf Sci Technol. 2018;2(1):18-23. doi: 10.11648/j.ajist.20180201.13

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@article{10.11648/j.ajist.20180201.13,
  author = {Anthony Kayode Onifade and Ogedegbe Gloria Palmer},
  title = {Plasmid Profile Analysis and Curing of Multidrug-Resistant Bacteria Isolated from Hospital Environmental Surfaces in Akure Metropolis, Ondo State, Nigeria},
  journal = {American Journal of Information Science and Technology},
  volume = {2},
  number = {1},
  pages = {18-23},
  doi = {10.11648/j.ajist.20180201.13},
  url = {https://doi.org/10.11648/j.ajist.20180201.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajist.20180201.13},
  abstract = {Plasmid profile analysis and curing of multidrug-resistant bacteria isolated from hospital environment in Akure metropolis, Ondo State Nigeria were investigated. Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Bacillus cereus were the bacterial isolated in the course of the research. The result revealed that Staphylococcus aureus and Pseudomonas aeruginosa showed multiple antibiotics resistance. Staphylococcus aureus and Pseudomonas aeruginosa are rapidly increasing as multidrug resistant strains worldwide. The results of antibiotics sensitivity test on the microorganisms subjected to plasmid analysis indicated that they were more susceptible to antibiotics after the plasmid curing of the microorganisms. The results from this investigative study have shown that resistance in S. aureus and P. aeruginosa are plasmid based as a result of its loss after curing.},
 year = {2018}
}

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TY  - JOUR
T1  - Plasmid Profile Analysis and Curing of Multidrug-Resistant Bacteria Isolated from Hospital Environmental Surfaces in Akure Metropolis, Ondo State, Nigeria
AU  - Anthony Kayode Onifade
AU  - Ogedegbe Gloria Palmer
Y1  - 2018/05/24
PY  - 2018
N1  - https://doi.org/10.11648/j.ajist.20180201.13
DO  - 10.11648/j.ajist.20180201.13
T2  - American Journal of Information Science and Technology
JF  - American Journal of Information Science and Technology
JO  - American Journal of Information Science and Technology
SP  - 18
EP  - 23
PB  - Science Publishing Group
SN  - 2640-0588
UR  - https://doi.org/10.11648/j.ajist.20180201.13
AB  - Plasmid profile analysis and curing of multidrug-resistant bacteria isolated from hospital environment in Akure metropolis, Ondo State Nigeria were investigated. Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Bacillus cereus were the bacterial isolated in the course of the research. The result revealed that Staphylococcus aureus and Pseudomonas aeruginosa showed multiple antibiotics resistance. Staphylococcus aureus and Pseudomonas aeruginosa are rapidly increasing as multidrug resistant strains worldwide. The results of antibiotics sensitivity test on the microorganisms subjected to plasmid analysis indicated that they were more susceptible to antibiotics after the plasmid curing of the microorganisms. The results from this investigative study have shown that resistance in S. aureus and P. aeruginosa are plasmid based as a result of its loss after curing.
VL  - 2
IS  - 1
ER  -

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Author Information

Anthony Kayode Onifade

Department of Microbiology, The Federal University of Technology, Akure, Nigeria
Ogedegbe Gloria Palmer

Department of Microbiology, The Federal University of Technology, Akure, Nigeria

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APA Style

Anthony Kayode Onifade, Ogedegbe Gloria Palmer. (2018). Plasmid Profile Analysis and Curing of Multidrug-Resistant Bacteria Isolated from Hospital Environmental Surfaces in Akure Metropolis, Ondo State, Nigeria. American Journal of Information Science and Technology, 2(1), 18-23. https://doi.org/10.11648/j.ajist.20180201.13

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ACS Style

Anthony Kayode Onifade; Ogedegbe Gloria Palmer. Plasmid Profile Analysis and Curing of Multidrug-Resistant Bacteria Isolated from Hospital Environmental Surfaces in Akure Metropolis, Ondo State, Nigeria. Am. J. Inf. Sci. Technol. 2018, 2(1), 18-23. doi: 10.11648/j.ajist.20180201.13

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AMA Style

Anthony Kayode Onifade, Ogedegbe Gloria Palmer. Plasmid Profile Analysis and Curing of Multidrug-Resistant Bacteria Isolated from Hospital Environmental Surfaces in Akure Metropolis, Ondo State, Nigeria. Am J Inf Sci Technol. 2018;2(1):18-23. doi: 10.11648/j.ajist.20180201.13

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@article{10.11648/j.ajist.20180201.13,
  author = {Anthony Kayode Onifade and Ogedegbe Gloria Palmer},
  title = {Plasmid Profile Analysis and Curing of Multidrug-Resistant Bacteria Isolated from Hospital Environmental Surfaces in Akure Metropolis, Ondo State, Nigeria},
  journal = {American Journal of Information Science and Technology},
  volume = {2},
  number = {1},
  pages = {18-23},
  doi = {10.11648/j.ajist.20180201.13},
  url = {https://doi.org/10.11648/j.ajist.20180201.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajist.20180201.13},
  abstract = {Plasmid profile analysis and curing of multidrug-resistant bacteria isolated from hospital environment in Akure metropolis, Ondo State Nigeria were investigated. Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Bacillus cereus were the bacterial isolated in the course of the research. The result revealed that Staphylococcus aureus and Pseudomonas aeruginosa showed multiple antibiotics resistance. Staphylococcus aureus and Pseudomonas aeruginosa are rapidly increasing as multidrug resistant strains worldwide. The results of antibiotics sensitivity test on the microorganisms subjected to plasmid analysis indicated that they were more susceptible to antibiotics after the plasmid curing of the microorganisms. The results from this investigative study have shown that resistance in S. aureus and P. aeruginosa are plasmid based as a result of its loss after curing.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Plasmid Profile Analysis and Curing of Multidrug-Resistant Bacteria Isolated from Hospital Environmental Surfaces in Akure Metropolis, Ondo State, Nigeria
AU  - Anthony Kayode Onifade
AU  - Ogedegbe Gloria Palmer
Y1  - 2018/05/24
PY  - 2018
N1  - https://doi.org/10.11648/j.ajist.20180201.13
DO  - 10.11648/j.ajist.20180201.13
T2  - American Journal of Information Science and Technology
JF  - American Journal of Information Science and Technology
JO  - American Journal of Information Science and Technology
SP  - 18
EP  - 23
PB  - Science Publishing Group
SN  - 2640-0588
UR  - https://doi.org/10.11648/j.ajist.20180201.13
AB  - Plasmid profile analysis and curing of multidrug-resistant bacteria isolated from hospital environment in Akure metropolis, Ondo State Nigeria were investigated. Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Bacillus cereus were the bacterial isolated in the course of the research. The result revealed that Staphylococcus aureus and Pseudomonas aeruginosa showed multiple antibiotics resistance. Staphylococcus aureus and Pseudomonas aeruginosa are rapidly increasing as multidrug resistant strains worldwide. The results of antibiotics sensitivity test on the microorganisms subjected to plasmid analysis indicated that they were more susceptible to antibiotics after the plasmid curing of the microorganisms. The results from this investigative study have shown that resistance in S. aureus and P. aeruginosa are plasmid based as a result of its loss after curing.
VL  - 2
IS  - 1
ER  -

Copy | Download