Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm

Pierrette Ngo Bahebeck; Claire Stephane Metsopkeng; Joelle Signe MBiada; Chrétien Lontsi Djimeli; Antoine Tamsa Arfao; Paul Alain Nana; Olive Vivien Noah Ewoti; Luciane Marlyse Moungang; Genevieve Bricheux; Télesphore Sime-Ngando; Moïse Nola

doi:doi:10.11648/j.ijmb.20190403.11

Assessment of the Effect of Light, HgCl₂ and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm

Pierrette Ngo Bahebeck, Claire Stephane Metsopkeng, Joelle Signe MBiada, Chrétien Lontsi Djimeli, Antoine Tamsa Arfao, Paul Alain Nana, Olive Vivien Noah Ewoti, Luciane Marlyse Moungang, Genevieve Bricheux, Télesphore Sime-Ngando, Moïse Nola

Published in International Journal of Microbiology and Biotechnology (Volume 4, Issue 3)

Received: 22 May 2019 Accepted: 28 June 2019 Published: 24 July 2019

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Abstract

The aim of this study was to determine the cultivability of E. faecalis and enteropathogenic E. coli (EPEC) under different light intensities in the presence of different concentrations of mercury chloride (HgCl₂) and biodegradable organic compound (BOC). Studied light intensities were 0, 500, 1000, 1500 and 2000 lux while studied HgCl₂ concentrations were 0.001, 0.01, 0.1 and 1μg/L. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The duration of incubation under light was 6 h. Results showed that E. faecalis and EPEC bacteria are inactivated by HgCl₂ irrespective of studied concentration. The cells inhibition percentage (CIP) of E. faecalis varied from 94.46% to 99.53% at 0.001μg/L, from 94.77% to 99.55% at 0.01μg/L, from 94.92% to 99.57% at 0.1μg/L and from 96.97% to 99.77% at 1μg/L of HgCl₂. For EPEC cells, the CIP fluctuated between 89.87% and 98.99%, between 90.67% and 99.14%, between 92.05% and 99.14% and 93.50% and 99.25% respectively in solutions containing 0.001, 0.01, 0.1 and 1µg/L of HgCl₂. The highest abundance was observed under 1500 lux for E. faecalis and 500 lux for EPEC. Exposure to light seemed to intensify the toxic action of HgCl₂ in the medium. Cells metabolism reactivations under 2000 lux for E. faecalis and 1000 lux for EPEC were nevertheless observed. The level of this photo-reactivation varies from one organism species to another. The oligotrophic nature of the medium could not allow studied the microorganisms to set up specific protection mechanism against HgCl₂ and light.

Published in International Journal of Microbiology and Biotechnology (Volume 4, Issue 3)

DOI 10.11648/j.ijmb.20190403.11

Page(s) 55-63

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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

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Keywords

Enterococcus faecalis, Escherichia coli, Light Intensity, Mercury Chloride, Biodegradable Organic Compound, Aquatic Microcosm

References

[1] Tshikantwa TS, Ullah MW, Feng He, Yang G. Current trends and potential applications of microbial interactions for human welfare. Front Microbiol, 2018; 9: 1156. doi: 10.3389/fmicb.2018.01156.

[2] Malla B, Shrestha RG, Tandukar S, Bhandari D, Inoue D, Sei K, Tanaka Y, Sherchand J B, Haramoto E. Identiﬁcation of human and animal fecal contamination in drinking water sources in the Kathmandu Valley, Nepal, using host-associated bacteroidales quantitative PCR assays. Water 2018; 10, 1796. doi: 10.3390/w10121796.

[3] Onyango AE, Okoth MW, Kunyanga CN, Ochieng’ Aliwa B. Microbiological quality and contamination level of water sources in isiolo county in Kenya. Journal of Environmental and Public Health, 2018; Article ID 2139867, 10 pages. doi: 10.1155/2018/2139867.

[4] Strauss A, Dobrowsky PH, Ndlovu T, Reyneke B, Khan W. Comparative analysis of solar pasteurization versus solar disinfection for the treatment of harvested rainwater. BMC Microbiol., 2016; 16(1): 289. doi: 10.1186/s12866-016-0909-y.

[5] Gomes J, Matos A, Gmurek M, Quinta-Ferreira RM, Martins RC. Ozone and photocatalytic processes for pathogens removal from water: A Review. Catalysts, 2019; 9(46). doi: 10.3390/catal9010046.

[6] Santos A, Moreirinha C, Lopes D, Esteves A, Henriques I, Almeida A, Domingues MR, Delgadillo I, Correia A, Cunha A. Effects of UV radiation on the lipids and proteins of bacteria studied by mid-infrared spectroscopy. Environmental Science and Technology, 2013; 47(12). doi: 10.1021/es400660g.

[7] Garcia-Pichel F. A model for internal shelf-shading in planktonic organisms and its implications for the usefulness of ultraviolet sunscreens. Limnol Oceanogr, 1994; 39: 1704-1717.

[8] Alonso-Saez L, Gasol JM, Lefort T, Hofer J, Sommaruga R. Effect of natural sunlight on bacterial activity and differential sensitivity of natural bacterioplankton groups in Northwestern Mediterranean coastal waters. Appl Environ Microbiol, 2006; 72(9): 5806–5813. doi: 10.1128/AEM.00597-06.

[9] Yang L, Lei Li. Insights into the activity change of spore photoproduct lyase induced by mutations at a peripheral glycine residue. Front. Chem., 2017; 5(4). https://doi.org/10.3389/fchem.2017.00014.

[10] Prabhakaran P, Ashraf MA, Aqma Wan WS. Microbial stress response to heavy metal in the environment. RSC Advances, 2016; 6(111). doi: 10.1039/C6RA10966G.

[11] Stadtman ER, Levine RL. Free radical-mediated oxidation of free amino acids and amino acid residues in proteins. Amino Acids, 2003; 25: 207-218. doi: 10.1007/s00726-003-0011-2.

[12] Stadtman ER. Oxidation of free amino acids and amino acid residues in proteins by radiolysis and by metal-catalyzed reactions. Annual Rev Biochem, 1993; 62: 797-821. doi: 10.1146/annurev.bi.62.070193.004053.

[13] Macomber L, Imlay JA. The iron-sulfur clusters of dehydratases are primary intracellular targets of copper toxicity. Proc Natl Acad Sci USA, 2009; 106:8344-8349. doi: 10.1073/pnas.0812808106.

[14] Nies DH. Microbial heavy-metal resistance. Appl Microbiol Biotechnol, 1999; 51:730-750. http://dx.doi.org.ezproxy.library.ubc.ca/10.1007/s002530051457.

[15] Nies DH, Silver S. Ion efflux systems involved in bacterial metal resistances. J Ind Microbiol, 1995; 14: 186-199.

[16] Holland SL, Ghosh E, Avery SV. Chromate-induced sulfur starvation and mRNA mistranslation in yeast are linked in a common mechanism of Cr toxicity. Toxicol in Vitro, 2010; 24:1764-1767. doi: 10.1016/j.tiv.2010.07.006.

[17] Hong R, Kang TY, Michels CA, Gadura N. Membrane lipid peroxidation in copper alloy-mediated contact killing of Escherichia coli. Appl Environ Microbiol, 2012; 78: 1776-1784. doi: 10.1128/AEM.07068-11.

[18] Dibrov P, Dzioba J, Gosink KK, Hase CC. Chemiosmotic mechanism of antimicrobial activity of Ag+ in Vibrio cholerae. Antimicrob Agents Chemother, 2002; 46: 2668-2670. doi: 10.1128/AAC.46.8.2668-2670.2002.

[19] Misha Y. Vaidya, Andrew J. McBain, Jonathan A. Butler, Craig E. Banks & Kathryn A. Whitehead. Antimicrobial efficacy and synergy of metal ions against Enterococcus faecium, Klebsiella pneumoniae and Acinetobacter baumannii in planktonic and biofilm phenotypes. Scientific Reports, 2017; volume 7, Article number: 5911.

[20] Keyer K, Imlay JA. Superoxide accelerates DNA damage by elevating free-iron levels. Proc Natl Acad Sci USA, 1996; 93: 13635-13640. www.pnas.org/content/93/24/13635.long.

[21] Lemire JA, Harrison JJ, Turner R. Antimicrobial activity of metals: mechanisms, molecular targets and applications. Nat Rev Micro, 2013; 11:371-384. doi: 10.1038/nrmicro3028.

[22] Warnes SL, Highmore CJ, Keevil CW. Horizontal transfer of antibiotic resistance genes on abiotic touch surfaces: Implications for public health. mBio, 2012; 3(6): e00489-12. doi: 10.1128/mBio.00489-12.

[23] Rodier J. L’analyse de l’eau. 9e ed, Dunod, Paris, 2009; 1579 p.

[24] Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergey’s Manual of determinative bacteriology. Lippincott Williams and Wilkins (edit), Philadelphia, 2000; 787p.

[25] APHA (American Public Health Association). Standard methods for the examination of water and waste water, APHA 22th Edition, Washington DC, 2012.

[26] Lipovsky A, Nitzan Y, Gedanken A, Lubart R. Visible light-induced killing of bacteria as a function of wavelength: implication for wound healing. Lasers Surg Med, 2010; 42(6):467-472. doi: 10.1002/lsm.20948.

[27] Kannaujiya VK, Sinha RP. Impacts of varying light regimes on phycobiliproteins of Nostoc sp. HKAR-2 and Nostoc sp. HKAR-11 isolated from diverse habitats. Protoplasma, 2015; 252(6): 1551-1561. doi: 10.1007/s00709-015-0786-5.

[28] Khajepour F, Hosseini SA, Ghorbani Nasrabadi R, Markou G. Effect of light intensity and photoperiod on growth and biochemical composition of a local isolate of Nostoc calcicola. Appl Biochem Biotechnol, 2015; 176(8): 2279-2289. doi: 10.1007/s12010-015-1717-9.

[29] Seiler C, Berendonk T. Heavy metal driven co-selection of antibiotic resistance in soil and water bodies impacted by agriculture and aquaculture. Frontiers in Microbiology, 2012; 3:1-10. doi: 10.3389/fmicb.2012.00399.

[30] Schmidtlein F, Lübken M, Grote I, Orth H, Wichern M. Photoreactivation and subsequent solar disinfection of Escherichia coli in UV-disinfected municipal wastewater under natural conditions. Water Science and Technology, 2015; 71(2): 220-226. doi: 10.2166/wst.2014.488.

[31] Shafaei S, Bolton JR, El Din MG. Effect of environmental factors on photoreactivation of microorganisms under indoor conditions. International Journal of Environmental and Ecological Engineering, 2016; 10(5): 560-563. doi.org/10.5281/zenodo.1124297.

[32] Abboudi M. Réponse des bactéries marines au rayonnement ultraviolet-visible et aux modifications photochimiques de la matière organique dissoute. Thèse de Doctorat en Océanologie & Microbiologie marine, Université Aix Marseille 2, France, 2007; 167 p.

[33] Deller S, Mascher F, Platzer S, Reinthaler FF, Egon M. Effect of solar radiation on survival of indicator bacteria in bathing waters. Cent Eur J Publ Health, 2006; 14(3): 133–137.

[34] Dahl T, Midden W, Hartman P. Comparison of killing of Gram-negative and Gram-positive bacteria by pure singlet oxygen. Journal of Applied Bacteriology, 1989; 171(4): 2188–2194. doi: 0021-9193/89/042188-07$02.00/0.

[35] Gupta P, Diwan B. Bacterial exopolysaccharide mediated heavy metal removal: A Review on biosynthesis, mechanism and remediation strategies. Biotechnology Reports, 2017; 13: 58-71. doi: 10.1016/j.btre.2016.12.006.

[36] Lotareva OV. Physiological effects of sunlight on Bacillus subtilis strains with varying capacity to repair photoproducts. Mikrobiologiia, 1996; 65(1): 74-78.

[37] Istvan Pocsi. Toxic metal/metalloid tolerance in fungi. A biotechnology-oriented approach. In Cellular effect of heavy metals. Gaspar Banfalvi (edit.), Springer Science+Business B. V., 2001; pp. 31-58.

[38] Dunkel N, Blass J, Rogers PD, Morschhauser J. Mutations in the multi-drug resistance regulator MRR1, followed by loss of heterozygosity, are the main cause of MDR1 overexpression in fluconazole-resistant Candida albicans strains. Mol Microbiol, 2008a; 69: 827-840. doi: 10.1111/j.1365-2958.2008.06309.x.

[39] Dunkel N, Liu TT, Barker KS, Homayouni R, Morschhäuser J, Roger PD. A Gain-of-function mutation in the transcription factor Upc2p causes upregulation of Ergosterol biosynthesis genes and increased Fluconazole resistance in a clinical Candida albicans isolate. Eukaryotic Cell, 2008b; 7(7): 1180-1190. doi: 10.1128/EC.00103-08.

[40] Marie C, White TC. Genetic basis of antifungal drug resistance. Curr Fungal Infect Rep, 2009; 3(3): 163-169. doi: 10.1007/s12281-009-0021-y.

[41] Lima Silva AA, Ribeiro de Carvalho MA, Souza SAL, Teixeira Dias PM. Silva Filho R, Meirelles Saramago C, Melo Bento C, Hofer E. Heavy metal tolerance (Cr, Ag and Hg) in bacteria isolated from sewage. Brazilian Journal of Microbiology, 2012; 43(4):620–1631. doi: 10.1590/S1517-838220120004000047.

[42] Shameer S, Paramageetham C. Heavy metal detoxification by different Bacillus species isolated from solar salterns. Scientifica, Article ID 319760, 2015; 8 pages http://dx.doi.org/10.1155/2015/319760.

[43] Joux F, Jeffrey WH, Lebaron P, Mitchell DL. Marine bacterial isolates display diverse responses to UV-B radiation. Appl Environ Microbiol, 1999; 65(9): 3820-3827. doi: 0099-2240/99/$04.0010.

[44] Gugala N, Vu D, Parkins MD, Turner RJ. Speciﬁcity in the susceptibilities of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus clinical isolates to six metal antimicrobials. Antibiotics, 2019; 8(51). doi: 10.3390/antibiotics8020051.

[45] Kotrba P, Doleckova L, Lorenzo V, Ruml T. Enhanced bioaccumulation of heavy metal ions by bacterial cells due to surface display of short metal binding peptides. Appl Environ Microbiol, 1999; 65(3): 1092–1098. PMID: 10049868 PMCID: PMC91149.

[46] Misra TK. Bacterial resistances to inorganic mercury salts and organomercurials. Plasmid, 1992; 27(1): 4-16.

[47] Wang D, Huang S, Liu P, He Y, Chen W, Hu Q, Wei T, Gan J, Ma J, Chen H. Structural analysis of the Hg(II)-regulatory protein Tn501 MerR from Pseudomonas aeruginosa. Scientific Report, 2016; 6:33391. doi: 10.1038/srep33391.

[48] Hobman JL, Crossman LC. Bacterial antimicrobial metal ion resistance. Journal of Medical Microbiology, 2014; 64:471-497. Doi: 10.1099/jmm.0.023036-0.

[49] Zulaiko E, Utomo AP, Prima A, Alami NH, Kusuwytasari ND, Shovitri M and Sembiring L. Diversity of heavy metal resistant bacteria fron Kalimas Surabaya: aphylogenetic taxonomy approach. AIP Conference Proceedings 1854, 020042, 2017; https://doi.org/10.1063/1.4985433.

[50] Chang S, Wu Z, Sun W, Shu H. The construction of an engineered bacterial strain and its application in accumulating mercury from wastewater. Applied Sciences, 2018; 8:1572. doi: 10.3390/app8091572.

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Pierrette Ngo Bahebeck, Claire Stephane Metsopkeng, Joelle Signe MBiada, Chrétien Lontsi Djimeli, Antoine Tamsa Arfao, et al. (2019). Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm. International Journal of Microbiology and Biotechnology, 4(3), 55-63. https://doi.org/10.11648/j.ijmb.20190403.11

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Pierrette Ngo Bahebeck; Claire Stephane Metsopkeng; Joelle Signe MBiada; Chrétien Lontsi Djimeli; Antoine Tamsa Arfao, et al. Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm. Int. J. Microbiol. Biotechnol. 2019, 4(3), 55-63. doi: 10.11648/j.ijmb.20190403.11

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Pierrette Ngo Bahebeck, Claire Stephane Metsopkeng, Joelle Signe MBiada, Chrétien Lontsi Djimeli, Antoine Tamsa Arfao, et al. Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm. Int J Microbiol Biotechnol. 2019;4(3):55-63. doi: 10.11648/j.ijmb.20190403.11

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@article{10.11648/j.ijmb.20190403.11, author = {Pierrette Ngo Bahebeck and Claire Stephane Metsopkeng and Joelle Signe MBiada and Chrétien Lontsi Djimeli and Antoine Tamsa Arfao and Paul Alain Nana and Olive Vivien Noah Ewoti and Luciane Marlyse Moungang and Genevieve Bricheux and Télesphore Sime-Ngando and Moïse Nola}, title = {Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm}, journal = {International Journal of Microbiology and Biotechnology}, volume = {4}, number = {3}, pages = {55-63}, doi = {10.11648/j.ijmb.20190403.11}, url = {https://doi.org/10.11648/j.ijmb.20190403.11}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20190403.11}, abstract = {The aim of this study was to determine the cultivability of E. faecalis and enteropathogenic E. coli (EPEC) under different light intensities in the presence of different concentrations of mercury chloride (HgCl2) and biodegradable organic compound (BOC). Studied light intensities were 0, 500, 1000, 1500 and 2000 lux while studied HgCl2 concentrations were 0.001, 0.01, 0.1 and 1μg/L. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The duration of incubation under light was 6 h. Results showed that E. faecalis and EPEC bacteria are inactivated by HgCl2 irrespective of studied concentration. The cells inhibition percentage (CIP) of E. faecalis varied from 94.46% to 99.53% at 0.001μg/L, from 94.77% to 99.55% at 0.01μg/L, from 94.92% to 99.57% at 0.1μg/L and from 96.97% to 99.77% at 1μg/L of HgCl2. For EPEC cells, the CIP fluctuated between 89.87% and 98.99%, between 90.67% and 99.14%, between 92.05% and 99.14% and 93.50% and 99.25% respectively in solutions containing 0.001, 0.01, 0.1 and 1µg/L of HgCl2. The highest abundance was observed under 1500 lux for E. faecalis and 500 lux for EPEC. Exposure to light seemed to intensify the toxic action of HgCl2 in the medium. Cells metabolism reactivations under 2000 lux for E. faecalis and 1000 lux for EPEC were nevertheless observed. The level of this photo-reactivation varies from one organism species to another. The oligotrophic nature of the medium could not allow studied the microorganisms to set up specific protection mechanism against HgCl2 and light.}, year = {2019} }

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TY - JOUR T1 - Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm AU - Pierrette Ngo Bahebeck AU - Claire Stephane Metsopkeng AU - Joelle Signe MBiada AU - Chrétien Lontsi Djimeli AU - Antoine Tamsa Arfao AU - Paul Alain Nana AU - Olive Vivien Noah Ewoti AU - Luciane Marlyse Moungang AU - Genevieve Bricheux AU - Télesphore Sime-Ngando AU - Moïse Nola Y1 - 2019/07/24 PY - 2019 N1 - https://doi.org/10.11648/j.ijmb.20190403.11 DO - 10.11648/j.ijmb.20190403.11 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 55 EP - 63 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20190403.11 AB - The aim of this study was to determine the cultivability of E. faecalis and enteropathogenic E. coli (EPEC) under different light intensities in the presence of different concentrations of mercury chloride (HgCl2) and biodegradable organic compound (BOC). Studied light intensities were 0, 500, 1000, 1500 and 2000 lux while studied HgCl2 concentrations were 0.001, 0.01, 0.1 and 1μg/L. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The duration of incubation under light was 6 h. Results showed that E. faecalis and EPEC bacteria are inactivated by HgCl2 irrespective of studied concentration. The cells inhibition percentage (CIP) of E. faecalis varied from 94.46% to 99.53% at 0.001μg/L, from 94.77% to 99.55% at 0.01μg/L, from 94.92% to 99.57% at 0.1μg/L and from 96.97% to 99.77% at 1μg/L of HgCl2. For EPEC cells, the CIP fluctuated between 89.87% and 98.99%, between 90.67% and 99.14%, between 92.05% and 99.14% and 93.50% and 99.25% respectively in solutions containing 0.001, 0.01, 0.1 and 1µg/L of HgCl2. The highest abundance was observed under 1500 lux for E. faecalis and 500 lux for EPEC. Exposure to light seemed to intensify the toxic action of HgCl2 in the medium. Cells metabolism reactivations under 2000 lux for E. faecalis and 1000 lux for EPEC were nevertheless observed. The level of this photo-reactivation varies from one organism species to another. The oligotrophic nature of the medium could not allow studied the microorganisms to set up specific protection mechanism against HgCl2 and light. VL - 4 IS - 3 ER -

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Pierrette Ngo Bahebeck

Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon

Claire Stephane Metsopkeng

Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon

Joelle Signe MBiada

Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon

Chrétien Lontsi Djimeli

Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon

Antoine Tamsa Arfao

Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon

Paul Alain Nana

Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon

Olive Vivien Noah Ewoti

Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon

Luciane Marlyse Moungang

Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon

Genevieve Bricheux

Laboratory 'Microorganisms: Genome and Environment', Clermont Auvergne University, Aubière Cedex, France

Télesphore Sime-Ngando

Laboratory 'Microorganisms: Genome and Environment', Clermont Auvergne University, Aubière Cedex, France

Moïse Nola

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Pierrette Ngo Bahebeck, Claire Stephane Metsopkeng, Joelle Signe MBiada, Chrétien Lontsi Djimeli, Antoine Tamsa Arfao, et al. (2019). Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm. International Journal of Microbiology and Biotechnology, 4(3), 55-63. https://doi.org/10.11648/j.ijmb.20190403.11

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Pierrette Ngo Bahebeck; Claire Stephane Metsopkeng; Joelle Signe MBiada; Chrétien Lontsi Djimeli; Antoine Tamsa Arfao, et al. Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm. Int. J. Microbiol. Biotechnol. 2019, 4(3), 55-63. doi: 10.11648/j.ijmb.20190403.11

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Pierrette Ngo Bahebeck, Claire Stephane Metsopkeng, Joelle Signe MBiada, Chrétien Lontsi Djimeli, Antoine Tamsa Arfao, et al. Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm. Int J Microbiol Biotechnol. 2019;4(3):55-63. doi: 10.11648/j.ijmb.20190403.11

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@article{10.11648/j.ijmb.20190403.11, author = {Pierrette Ngo Bahebeck and Claire Stephane Metsopkeng and Joelle Signe MBiada and Chrétien Lontsi Djimeli and Antoine Tamsa Arfao and Paul Alain Nana and Olive Vivien Noah Ewoti and Luciane Marlyse Moungang and Genevieve Bricheux and Télesphore Sime-Ngando and Moïse Nola}, title = {Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm}, journal = {International Journal of Microbiology and Biotechnology}, volume = {4}, number = {3}, pages = {55-63}, doi = {10.11648/j.ijmb.20190403.11}, url = {https://doi.org/10.11648/j.ijmb.20190403.11}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20190403.11}, abstract = {The aim of this study was to determine the cultivability of E. faecalis and enteropathogenic E. coli (EPEC) under different light intensities in the presence of different concentrations of mercury chloride (HgCl2) and biodegradable organic compound (BOC). Studied light intensities were 0, 500, 1000, 1500 and 2000 lux while studied HgCl2 concentrations were 0.001, 0.01, 0.1 and 1μg/L. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The duration of incubation under light was 6 h. Results showed that E. faecalis and EPEC bacteria are inactivated by HgCl2 irrespective of studied concentration. The cells inhibition percentage (CIP) of E. faecalis varied from 94.46% to 99.53% at 0.001μg/L, from 94.77% to 99.55% at 0.01μg/L, from 94.92% to 99.57% at 0.1μg/L and from 96.97% to 99.77% at 1μg/L of HgCl2. For EPEC cells, the CIP fluctuated between 89.87% and 98.99%, between 90.67% and 99.14%, between 92.05% and 99.14% and 93.50% and 99.25% respectively in solutions containing 0.001, 0.01, 0.1 and 1µg/L of HgCl2. The highest abundance was observed under 1500 lux for E. faecalis and 500 lux for EPEC. Exposure to light seemed to intensify the toxic action of HgCl2 in the medium. Cells metabolism reactivations under 2000 lux for E. faecalis and 1000 lux for EPEC were nevertheless observed. The level of this photo-reactivation varies from one organism species to another. The oligotrophic nature of the medium could not allow studied the microorganisms to set up specific protection mechanism against HgCl2 and light.}, year = {2019} }

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TY - JOUR T1 - Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm AU - Pierrette Ngo Bahebeck AU - Claire Stephane Metsopkeng AU - Joelle Signe MBiada AU - Chrétien Lontsi Djimeli AU - Antoine Tamsa Arfao AU - Paul Alain Nana AU - Olive Vivien Noah Ewoti AU - Luciane Marlyse Moungang AU - Genevieve Bricheux AU - Télesphore Sime-Ngando AU - Moïse Nola Y1 - 2019/07/24 PY - 2019 N1 - https://doi.org/10.11648/j.ijmb.20190403.11 DO - 10.11648/j.ijmb.20190403.11 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 55 EP - 63 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20190403.11 AB - The aim of this study was to determine the cultivability of E. faecalis and enteropathogenic E. coli (EPEC) under different light intensities in the presence of different concentrations of mercury chloride (HgCl2) and biodegradable organic compound (BOC). Studied light intensities were 0, 500, 1000, 1500 and 2000 lux while studied HgCl2 concentrations were 0.001, 0.01, 0.1 and 1μg/L. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The duration of incubation under light was 6 h. Results showed that E. faecalis and EPEC bacteria are inactivated by HgCl2 irrespective of studied concentration. The cells inhibition percentage (CIP) of E. faecalis varied from 94.46% to 99.53% at 0.001μg/L, from 94.77% to 99.55% at 0.01μg/L, from 94.92% to 99.57% at 0.1μg/L and from 96.97% to 99.77% at 1μg/L of HgCl2. For EPEC cells, the CIP fluctuated between 89.87% and 98.99%, between 90.67% and 99.14%, between 92.05% and 99.14% and 93.50% and 99.25% respectively in solutions containing 0.001, 0.01, 0.1 and 1µg/L of HgCl2. The highest abundance was observed under 1500 lux for E. faecalis and 500 lux for EPEC. Exposure to light seemed to intensify the toxic action of HgCl2 in the medium. Cells metabolism reactivations under 2000 lux for E. faecalis and 1000 lux for EPEC were nevertheless observed. The level of this photo-reactivation varies from one organism species to another. The oligotrophic nature of the medium could not allow studied the microorganisms to set up specific protection mechanism against HgCl2 and light. VL - 4 IS - 3 ER -

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Published in	International Journal of Microbiology and Biotechnology (Volume 4, Issue 3)
DOI	10.11648/j.ijmb.20190403.11
Page(s)	55-63
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

[1]	Tshikantwa TS, Ullah MW, Feng He, Yang G. Current trends and potential applications of microbial interactions for human welfare. Front Microbiol, 2018; 9: 1156. doi: 10.3389/fmicb.2018.01156.
[2]	Malla B, Shrestha RG, Tandukar S, Bhandari D, Inoue D, Sei K, Tanaka Y, Sherchand J B, Haramoto E. Identiﬁcation of human and animal fecal contamination in drinking water sources in the Kathmandu Valley, Nepal, using host-associated bacteroidales quantitative PCR assays. Water 2018; 10, 1796. doi: 10.3390/w10121796.
[3]	Onyango AE, Okoth MW, Kunyanga CN, Ochieng’ Aliwa B. Microbiological quality and contamination level of water sources in isiolo county in Kenya. Journal of Environmental and Public Health, 2018; Article ID 2139867, 10 pages. doi: 10.1155/2018/2139867.
[4]	Strauss A, Dobrowsky PH, Ndlovu T, Reyneke B, Khan W. Comparative analysis of solar pasteurization versus solar disinfection for the treatment of harvested rainwater. BMC Microbiol., 2016; 16(1): 289. doi: 10.1186/s12866-016-0909-y.
[5]	Gomes J, Matos A, Gmurek M, Quinta-Ferreira RM, Martins RC. Ozone and photocatalytic processes for pathogens removal from water: A Review. Catalysts, 2019; 9(46). doi: 10.3390/catal9010046.
[6]	Santos A, Moreirinha C, Lopes D, Esteves A, Henriques I, Almeida A, Domingues MR, Delgadillo I, Correia A, Cunha A. Effects of UV radiation on the lipids and proteins of bacteria studied by mid-infrared spectroscopy. Environmental Science and Technology, 2013; 47(12). doi: 10.1021/es400660g.
[7]	Garcia-Pichel F. A model for internal shelf-shading in planktonic organisms and its implications for the usefulness of ultraviolet sunscreens. Limnol Oceanogr, 1994; 39: 1704-1717.
[8]	Alonso-Saez L, Gasol JM, Lefort T, Hofer J, Sommaruga R. Effect of natural sunlight on bacterial activity and differential sensitivity of natural bacterioplankton groups in Northwestern Mediterranean coastal waters. Appl Environ Microbiol, 2006; 72(9): 5806–5813. doi: 10.1128/AEM.00597-06.
[9]	Yang L, Lei Li. Insights into the activity change of spore photoproduct lyase induced by mutations at a peripheral glycine residue. Front. Chem., 2017; 5(4). https://doi.org/10.3389/fchem.2017.00014.
[10]	Prabhakaran P, Ashraf MA, Aqma Wan WS. Microbial stress response to heavy metal in the environment. RSC Advances, 2016; 6(111). doi: 10.1039/C6RA10966G.
[11]	Stadtman ER, Levine RL. Free radical-mediated oxidation of free amino acids and amino acid residues in proteins. Amino Acids, 2003; 25: 207-218. doi: 10.1007/s00726-003-0011-2.
[12]	Stadtman ER. Oxidation of free amino acids and amino acid residues in proteins by radiolysis and by metal-catalyzed reactions. Annual Rev Biochem, 1993; 62: 797-821. doi: 10.1146/annurev.bi.62.070193.004053.
[13]	Macomber L, Imlay JA. The iron-sulfur clusters of dehydratases are primary intracellular targets of copper toxicity. Proc Natl Acad Sci USA, 2009; 106:8344-8349. doi: 10.1073/pnas.0812808106.
[14]	Nies DH. Microbial heavy-metal resistance. Appl Microbiol Biotechnol, 1999; 51:730-750. http://dx.doi.org.ezproxy.library.ubc.ca/10.1007/s002530051457.
[15]	Nies DH, Silver S. Ion efflux systems involved in bacterial metal resistances. J Ind Microbiol, 1995; 14: 186-199.
[16]	Holland SL, Ghosh E, Avery SV. Chromate-induced sulfur starvation and mRNA mistranslation in yeast are linked in a common mechanism of Cr toxicity. Toxicol in Vitro, 2010; 24:1764-1767. doi: 10.1016/j.tiv.2010.07.006.
[17]	Hong R, Kang TY, Michels CA, Gadura N. Membrane lipid peroxidation in copper alloy-mediated contact killing of Escherichia coli. Appl Environ Microbiol, 2012; 78: 1776-1784. doi: 10.1128/AEM.07068-11.
[18]	Dibrov P, Dzioba J, Gosink KK, Hase CC. Chemiosmotic mechanism of antimicrobial activity of Ag+ in Vibrio cholerae. Antimicrob Agents Chemother, 2002; 46: 2668-2670. doi: 10.1128/AAC.46.8.2668-2670.2002.
[19]	Misha Y. Vaidya, Andrew J. McBain, Jonathan A. Butler, Craig E. Banks & Kathryn A. Whitehead. Antimicrobial efficacy and synergy of metal ions against Enterococcus faecium, Klebsiella pneumoniae and Acinetobacter baumannii in planktonic and biofilm phenotypes. Scientific Reports, 2017; volume 7, Article number: 5911.
[20]	Keyer K, Imlay JA. Superoxide accelerates DNA damage by elevating free-iron levels. Proc Natl Acad Sci USA, 1996; 93: 13635-13640. www.pnas.org/content/93/24/13635.long.
[21]	Lemire JA, Harrison JJ, Turner R. Antimicrobial activity of metals: mechanisms, molecular targets and applications. Nat Rev Micro, 2013; 11:371-384. doi: 10.1038/nrmicro3028.
[22]	Warnes SL, Highmore CJ, Keevil CW. Horizontal transfer of antibiotic resistance genes on abiotic touch surfaces: Implications for public health. mBio, 2012; 3(6): e00489-12. doi: 10.1128/mBio.00489-12.
[23]	Rodier J. L’analyse de l’eau. 9e ed, Dunod, Paris, 2009; 1579 p.
[24]	Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergey’s Manual of determinative bacteriology. Lippincott Williams and Wilkins (edit), Philadelphia, 2000; 787p.
[25]	APHA (American Public Health Association). Standard methods for the examination of water and waste water, APHA 22th Edition, Washington DC, 2012.
[26]	Lipovsky A, Nitzan Y, Gedanken A, Lubart R. Visible light-induced killing of bacteria as a function of wavelength: implication for wound healing. Lasers Surg Med, 2010; 42(6):467-472. doi: 10.1002/lsm.20948.
[27]	Kannaujiya VK, Sinha RP. Impacts of varying light regimes on phycobiliproteins of Nostoc sp. HKAR-2 and Nostoc sp. HKAR-11 isolated from diverse habitats. Protoplasma, 2015; 252(6): 1551-1561. doi: 10.1007/s00709-015-0786-5.
[28]	Khajepour F, Hosseini SA, Ghorbani Nasrabadi R, Markou G. Effect of light intensity and photoperiod on growth and biochemical composition of a local isolate of Nostoc calcicola. Appl Biochem Biotechnol, 2015; 176(8): 2279-2289. doi: 10.1007/s12010-015-1717-9.
[29]	Seiler C, Berendonk T. Heavy metal driven co-selection of antibiotic resistance in soil and water bodies impacted by agriculture and aquaculture. Frontiers in Microbiology, 2012; 3:1-10. doi: 10.3389/fmicb.2012.00399.
[30]	Schmidtlein F, Lübken M, Grote I, Orth H, Wichern M. Photoreactivation and subsequent solar disinfection of Escherichia coli in UV-disinfected municipal wastewater under natural conditions. Water Science and Technology, 2015; 71(2): 220-226. doi: 10.2166/wst.2014.488.
[31]	Shafaei S, Bolton JR, El Din MG. Effect of environmental factors on photoreactivation of microorganisms under indoor conditions. International Journal of Environmental and Ecological Engineering, 2016; 10(5): 560-563. doi.org/10.5281/zenodo.1124297.
[32]	Abboudi M. Réponse des bactéries marines au rayonnement ultraviolet-visible et aux modifications photochimiques de la matière organique dissoute. Thèse de Doctorat en Océanologie & Microbiologie marine, Université Aix Marseille 2, France, 2007; 167 p.
[33]	Deller S, Mascher F, Platzer S, Reinthaler FF, Egon M. Effect of solar radiation on survival of indicator bacteria in bathing waters. Cent Eur J Publ Health, 2006; 14(3): 133–137.
[34]	Dahl T, Midden W, Hartman P. Comparison of killing of Gram-negative and Gram-positive bacteria by pure singlet oxygen. Journal of Applied Bacteriology, 1989; 171(4): 2188–2194. doi: 0021-9193/89/042188-07$02.00/0.
[35]	Gupta P, Diwan B. Bacterial exopolysaccharide mediated heavy metal removal: A Review on biosynthesis, mechanism and remediation strategies. Biotechnology Reports, 2017; 13: 58-71. doi: 10.1016/j.btre.2016.12.006.
[36]	Lotareva OV. Physiological effects of sunlight on Bacillus subtilis strains with varying capacity to repair photoproducts. Mikrobiologiia, 1996; 65(1): 74-78.
[37]	Istvan Pocsi. Toxic metal/metalloid tolerance in fungi. A biotechnology-oriented approach. In Cellular effect of heavy metals. Gaspar Banfalvi (edit.), Springer Science+Business B. V., 2001; pp. 31-58.
[38]	Dunkel N, Blass J, Rogers PD, Morschhauser J. Mutations in the multi-drug resistance regulator MRR1, followed by loss of heterozygosity, are the main cause of MDR1 overexpression in fluconazole-resistant Candida albicans strains. Mol Microbiol, 2008a; 69: 827-840. doi: 10.1111/j.1365-2958.2008.06309.x.
[39]	Dunkel N, Liu TT, Barker KS, Homayouni R, Morschhäuser J, Roger PD. A Gain-of-function mutation in the transcription factor Upc2p causes upregulation of Ergosterol biosynthesis genes and increased Fluconazole resistance in a clinical Candida albicans isolate. Eukaryotic Cell, 2008b; 7(7): 1180-1190. doi: 10.1128/EC.00103-08.
[40]	Marie C, White TC. Genetic basis of antifungal drug resistance. Curr Fungal Infect Rep, 2009; 3(3): 163-169. doi: 10.1007/s12281-009-0021-y.
[41]	Lima Silva AA, Ribeiro de Carvalho MA, Souza SAL, Teixeira Dias PM. Silva Filho R, Meirelles Saramago C, Melo Bento C, Hofer E. Heavy metal tolerance (Cr, Ag and Hg) in bacteria isolated from sewage. Brazilian Journal of Microbiology, 2012; 43(4):620–1631. doi: 10.1590/S1517-838220120004000047.
[42]	Shameer S, Paramageetham C. Heavy metal detoxification by different Bacillus species isolated from solar salterns. Scientifica, Article ID 319760, 2015; 8 pages http://dx.doi.org/10.1155/2015/319760.
[43]	Joux F, Jeffrey WH, Lebaron P, Mitchell DL. Marine bacterial isolates display diverse responses to UV-B radiation. Appl Environ Microbiol, 1999; 65(9): 3820-3827. doi: 0099-2240/99/$04.0010.
[44]	Gugala N, Vu D, Parkins MD, Turner RJ. Speciﬁcity in the susceptibilities of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus clinical isolates to six metal antimicrobials. Antibiotics, 2019; 8(51). doi: 10.3390/antibiotics8020051.
[45]	Kotrba P, Doleckova L, Lorenzo V, Ruml T. Enhanced bioaccumulation of heavy metal ions by bacterial cells due to surface display of short metal binding peptides. Appl Environ Microbiol, 1999; 65(3): 1092–1098. PMID: 10049868 PMCID: PMC91149.
[46]	Misra TK. Bacterial resistances to inorganic mercury salts and organomercurials. Plasmid, 1992; 27(1): 4-16.
[47]	Wang D, Huang S, Liu P, He Y, Chen W, Hu Q, Wei T, Gan J, Ma J, Chen H. Structural analysis of the Hg(II)-regulatory protein Tn501 MerR from Pseudomonas aeruginosa. Scientific Report, 2016; 6:33391. doi: 10.1038/srep33391.
[48]	Hobman JL, Crossman LC. Bacterial antimicrobial metal ion resistance. Journal of Medical Microbiology, 2014; 64:471-497. Doi: 10.1099/jmm.0.023036-0.
[49]	Zulaiko E, Utomo AP, Prima A, Alami NH, Kusuwytasari ND, Shovitri M and Sembiring L. Diversity of heavy metal resistant bacteria fron Kalimas Surabaya: aphylogenetic taxonomy approach. AIP Conference Proceedings 1854, 020042, 2017; https://doi.org/10.1063/1.4985433.
[50]	Chang S, Wu Z, Sun W, Shu H. The construction of an engineered bacterial strain and its application in accumulating mercury from wastewater. Applied Sciences, 2018; 8:1572. doi: 10.3390/app8091572.