American Journal of Pediatrics

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Expression of Progranulin in a Mouse Model of Newborn Hypoxic-ischemic Brain Damage

Received: 26 June 2019    Accepted: 30 July 2019    Published: 26 August 2019
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Abstract

Neonatal hypoxic ischemic encephalopathy (HIE) is one of the main reasons of death and disability in neonatal, for lack of blood and oxygen during the time of birth. Progranulin (PGRN) as a neurotrophic factor is extensively expressed in the brain can regulate neurite growth and promote neuronal survival. The mutations of PGRN gene may contribute to frontotemporal dementia (FTD). However, the role of PGRN in neonatal HIE remains unclear. We designed this study to investigate the changes of PGRN expression in the brain of newborn mice at different time points after hypoxic -ischemic brain damage (HIBD). Postnatal 7day (P7) mouse pups were induced HIBD model by the method of Rice with some improvement. TTC was used to detect the ischemic lesion volume. The localization of PGRN brain cells was detected by immunofluorescence. We also used Western blotting to measure the expression level of PGRN at different days (1, 3, 7 days) following HIBD. The results showed that we established the HIIBD model successfully. PGRN was primarily expressed in neurons and microglia, but rarely in astrocytes. In addition, PGRN expression in the brain of HIBD mice markedly increased at 1 day and 3 days and was restored at 7 days after HIBD. The results indicated that increased PGRN levels may be involved in the pathological mechanism and neural repair process of HIBD.

DOI 10.11648/j.ajp.20190503.23
Published in American Journal of Pediatrics (Volume 5, Issue 3, September 2019)
Page(s) 152-158
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Progranulin, Hypoxia-Ischemic Brain Damage, Neonatal Mice

References
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Author Information
  • School of Clinical Medicine, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China; School of Clinical Medicine, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, People's Republic of China

  • Guangzhou Women and Children's Medical Center, Guangzhou, People's Republic of China

  • School of Clinical Medicine, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China; School of Clinical Medicine, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, People's Republic of China

  • School of Clinical Medicine, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China; School of Clinical Medicine, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, People's Republic of China

  • School of Clinical Medicine, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China; School of Clinical Medicine, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, People's Republic of China

  • School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China

  • School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China

  • School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China

  • School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China

  • Intensive Care Unit, Guangdong No. 2 Provincial People's Hospital, Guangzhou, People's Republic of China

  • School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China

Cite This Article
  • APA Style

    Xuxin Ren, Qiaoli Ren, Yilin Liu, Wenyan Zhao, Wei Liu, et al. (2019). Expression of Progranulin in a Mouse Model of Newborn Hypoxic-ischemic Brain Damage. American Journal of Pediatrics, 5(3), 152-158. https://doi.org/10.11648/j.ajp.20190503.23

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    ACS Style

    Xuxin Ren; Qiaoli Ren; Yilin Liu; Wenyan Zhao; Wei Liu, et al. Expression of Progranulin in a Mouse Model of Newborn Hypoxic-ischemic Brain Damage. Am. J. Pediatr. 2019, 5(3), 152-158. doi: 10.11648/j.ajp.20190503.23

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    AMA Style

    Xuxin Ren, Qiaoli Ren, Yilin Liu, Wenyan Zhao, Wei Liu, et al. Expression of Progranulin in a Mouse Model of Newborn Hypoxic-ischemic Brain Damage. Am J Pediatr. 2019;5(3):152-158. doi: 10.11648/j.ajp.20190503.23

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  • @article{10.11648/j.ajp.20190503.23,
      author = {Xuxin Ren and Qiaoli Ren and Yilin Liu and Wenyan Zhao and Wei Liu and Xing Tu and Junhua Yang and Jing Liu and Guoying Li and Mengxia Wang and Li Luo},
      title = {Expression of Progranulin in a Mouse Model of Newborn Hypoxic-ischemic Brain Damage},
      journal = {American Journal of Pediatrics},
      volume = {5},
      number = {3},
      pages = {152-158},
      doi = {10.11648/j.ajp.20190503.23},
      url = {https://doi.org/10.11648/j.ajp.20190503.23},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajp.20190503.23},
      abstract = {Neonatal hypoxic ischemic encephalopathy (HIE) is one of the main reasons of death and disability in neonatal, for lack of blood and oxygen during the time of birth. Progranulin (PGRN) as a neurotrophic factor is extensively expressed in the brain can regulate neurite growth and promote neuronal survival. The mutations of PGRN gene may contribute to frontotemporal dementia (FTD). However, the role of PGRN in neonatal HIE remains unclear. We designed this study to investigate the changes of PGRN expression in the brain of newborn mice at different time points after hypoxic -ischemic brain damage (HIBD). Postnatal 7day (P7) mouse pups were induced HIBD model by the method of Rice with some improvement. TTC was used to detect the ischemic lesion volume. The localization of PGRN brain cells was detected by immunofluorescence. We also used Western blotting to measure the expression level of PGRN at different days (1, 3, 7 days) following HIBD. The results showed that we established the HIIBD model successfully. PGRN was primarily expressed in neurons and microglia, but rarely in astrocytes. In addition, PGRN expression in the brain of HIBD mice markedly increased at 1 day and 3 days and was restored at 7 days after HIBD. The results indicated that increased PGRN levels may be involved in the pathological mechanism and neural repair process of HIBD.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Expression of Progranulin in a Mouse Model of Newborn Hypoxic-ischemic Brain Damage
    AU  - Xuxin Ren
    AU  - Qiaoli Ren
    AU  - Yilin Liu
    AU  - Wenyan Zhao
    AU  - Wei Liu
    AU  - Xing Tu
    AU  - Junhua Yang
    AU  - Jing Liu
    AU  - Guoying Li
    AU  - Mengxia Wang
    AU  - Li Luo
    Y1  - 2019/08/26
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ajp.20190503.23
    DO  - 10.11648/j.ajp.20190503.23
    T2  - American Journal of Pediatrics
    JF  - American Journal of Pediatrics
    JO  - American Journal of Pediatrics
    SP  - 152
    EP  - 158
    PB  - Science Publishing Group
    SN  - 2472-0909
    UR  - https://doi.org/10.11648/j.ajp.20190503.23
    AB  - Neonatal hypoxic ischemic encephalopathy (HIE) is one of the main reasons of death and disability in neonatal, for lack of blood and oxygen during the time of birth. Progranulin (PGRN) as a neurotrophic factor is extensively expressed in the brain can regulate neurite growth and promote neuronal survival. The mutations of PGRN gene may contribute to frontotemporal dementia (FTD). However, the role of PGRN in neonatal HIE remains unclear. We designed this study to investigate the changes of PGRN expression in the brain of newborn mice at different time points after hypoxic -ischemic brain damage (HIBD). Postnatal 7day (P7) mouse pups were induced HIBD model by the method of Rice with some improvement. TTC was used to detect the ischemic lesion volume. The localization of PGRN brain cells was detected by immunofluorescence. We also used Western blotting to measure the expression level of PGRN at different days (1, 3, 7 days) following HIBD. The results showed that we established the HIIBD model successfully. PGRN was primarily expressed in neurons and microglia, but rarely in astrocytes. In addition, PGRN expression in the brain of HIBD mice markedly increased at 1 day and 3 days and was restored at 7 days after HIBD. The results indicated that increased PGRN levels may be involved in the pathological mechanism and neural repair process of HIBD.
    VL  - 5
    IS  - 3
    ER  - 

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