Objective: To investigate the neuroprotective effects of grape seed proanthocyanidin extract（GSPE）on a model of neonatal hypoxic ischemic encephalopathy (HIE), we investigated the changes in neuronal cells and astrocytes after pre-treatment with GSPE. Methods: Seven-day-old pups were randomly divided into sham, HI, and GSPE+HI groups. The HIE model was established using a modified Rice-Vannucci method. GSPE was injected intraperitoneally 20 min before surgery. The change in markers of neuronal cells and astrocytes (NeuN/GFAP) were detected by immunofluorescence and Western blot. Results: Compared with the sham group, the expression of NeuN in the HI group was significantly reduced, and the expression of NeuN was significantly increased after GSPE pre-treatment. The expression of GFAP was opposite to NeuN. Conclusion: Our study showed that GSPE pre-treatment significantly protected neurons and inhibited astrocyte over-proliferation. Therefore, we believe that GSPE is a potential drug for the treatment of HIE and can prevent brain damage caused by hypoxia and ischemia.
| Published in | American Journal of Pediatrics (Volume 5, Issue 2) |
| DOI | 10.11648/j.ajp.20190502.13 |
| Page(s) | 51-55 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Grape Seed Proanthocyanidin Extract, NeuN, GFAP, Neonatal Hypoxic-Ischemic Brain Injury
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APA Style
Li Luo, Xing Tu, Xuexia Zhang, Jing Liu, Yilin Liu, et al. (2019). Grape Seed Proanthocyanidin Extract Adjusts NeuN/GFAP in a Murine Model of Neonatal Hypoxic-ischemic Brain Injury. American Journal of Pediatrics, 5(2), 51-55. https://doi.org/10.11648/j.ajp.20190502.13
ACS Style
Li Luo; Xing Tu; Xuexia Zhang; Jing Liu; Yilin Liu, et al. Grape Seed Proanthocyanidin Extract Adjusts NeuN/GFAP in a Murine Model of Neonatal Hypoxic-ischemic Brain Injury. Am. J. Pediatr. 2019, 5(2), 51-55. doi: 10.11648/j.ajp.20190502.13
AMA Style
Li Luo, Xing Tu, Xuexia Zhang, Jing Liu, Yilin Liu, et al. Grape Seed Proanthocyanidin Extract Adjusts NeuN/GFAP in a Murine Model of Neonatal Hypoxic-ischemic Brain Injury. Am J Pediatr. 2019;5(2):51-55. doi: 10.11648/j.ajp.20190502.13
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Download@article{10.11648/j.ajp.20190502.13,
author = {Li Luo and Xing Tu and Xuexia Zhang and Jing Liu and Yilin Liu and Wenyan Zhao and Ke Niu and Junhua Yang and Guoying Li and Mengxia Wang},
title = {Grape Seed Proanthocyanidin Extract Adjusts NeuN/GFAP in a Murine Model of Neonatal Hypoxic-ischemic Brain Injury},
journal = {American Journal of Pediatrics},
volume = {5},
number = {2},
pages = {51-55},
doi = {10.11648/j.ajp.20190502.13},
url = {https://doi.org/10.11648/j.ajp.20190502.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajp.20190502.13},
abstract = {Objective: To investigate the neuroprotective effects of grape seed proanthocyanidin extract（GSPE）on a model of neonatal hypoxic ischemic encephalopathy (HIE), we investigated the changes in neuronal cells and astrocytes after pre-treatment with GSPE. Methods: Seven-day-old pups were randomly divided into sham, HI, and GSPE+HI groups. The HIE model was established using a modified Rice-Vannucci method. GSPE was injected intraperitoneally 20 min before surgery. The change in markers of neuronal cells and astrocytes (NeuN/GFAP) were detected by immunofluorescence and Western blot. Results: Compared with the sham group, the expression of NeuN in the HI group was significantly reduced, and the expression of NeuN was significantly increased after GSPE pre-treatment. The expression of GFAP was opposite to NeuN. Conclusion: Our study showed that GSPE pre-treatment significantly protected neurons and inhibited astrocyte over-proliferation. Therefore, we believe that GSPE is a potential drug for the treatment of HIE and can prevent brain damage caused by hypoxia and ischemia.},
year = {2019}
}
TY - JOUR T1 - Grape Seed Proanthocyanidin Extract Adjusts NeuN/GFAP in a Murine Model of Neonatal Hypoxic-ischemic Brain Injury AU - Li Luo AU - Xing Tu AU - Xuexia Zhang AU - Jing Liu AU - Yilin Liu AU - Wenyan Zhao AU - Ke Niu AU - Junhua Yang AU - Guoying Li AU - Mengxia Wang Y1 - 2019/05/10 PY - 2019 N1 - https://doi.org/10.11648/j.ajp.20190502.13 DO - 10.11648/j.ajp.20190502.13 T2 - American Journal of Pediatrics JF - American Journal of Pediatrics JO - American Journal of Pediatrics SP - 51 EP - 55 PB - Science Publishing Group SN - 2472-0909 UR - https://doi.org/10.11648/j.ajp.20190502.13 AB - Objective: To investigate the neuroprotective effects of grape seed proanthocyanidin extract（GSPE）on a model of neonatal hypoxic ischemic encephalopathy (HIE), we investigated the changes in neuronal cells and astrocytes after pre-treatment with GSPE. Methods: Seven-day-old pups were randomly divided into sham, HI, and GSPE+HI groups. The HIE model was established using a modified Rice-Vannucci method. GSPE was injected intraperitoneally 20 min before surgery. The change in markers of neuronal cells and astrocytes (NeuN/GFAP) were detected by immunofluorescence and Western blot. Results: Compared with the sham group, the expression of NeuN in the HI group was significantly reduced, and the expression of NeuN was significantly increased after GSPE pre-treatment. The expression of GFAP was opposite to NeuN. Conclusion: Our study showed that GSPE pre-treatment significantly protected neurons and inhibited astrocyte over-proliferation. Therefore, we believe that GSPE is a potential drug for the treatment of HIE and can prevent brain damage caused by hypoxia and ischemia. VL - 5 IS - 2 ER -
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