Digital Profile of Klebsiella Pneumoniae Strains Producing Extended Spectrum β-Lactamase Isolated in Hospitals in the Littoral Region of Cameroon

Jean Teghonong; Cecile Okalla Ebongue; Callixte Yadufashije; Dieudonne Adiogo

doi:doi:10.11648/j.ejcbs.20190503.12

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Digital Profile of Klebsiella Pneumoniae Strains Producing Extended Spectrum β-Lactamase Isolated in Hospitals in the Littoral Region of Cameroon

Jean Teghonong, Cecile Okalla Ebongue, Callixte Yadufashije, Dieudonne Adiogo

Published in European Journal of Clinical and Biomedical Sciences (Volume 5, Issue 3)

Received: 24 May 2019 Accepted: 1 July 2019 Published: 24 July 2019

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Abstract

Miniaturized galleries are essential for a good identification of bacteria. The aim of this study was to identify multi-resistant Klebsiellap neumoniae strains in the referral health structures of Cameroon littoral region and establish a digital profile. A cross-sectional and descriptivestudy was conducted during 2016 and early 2017, intermediate susceptibility isolates or resistant to cephalosporins third generation (C3G) were collected in the laboratories of referral hospitals of the Littoral (General Hospital, Laquintinie, Military, Gyneco-obstetrics and pediatrics of Douala and Saint John of Malta of Njombé) and in Diagmed laboratory. The confirmation of the strains was made by the gallery API 20E™ (BioMérieux). In this study, atotal of 412 strains of Klebsiellasp (species) were isolated. We obtained 125 strains of Klebsiellasp intermediate or resistant to (C3G) and 122 were confirmed to be Klebsiella pneumoniae. The sex ratio (F / M) was 1.26. Klebsiella pneumoniae pneumonia was the species mostly identified at 82.4% and the two digital profiles mainly found were 5215773 and 1215773 with respective frequencies of 52.8% and 18.4%. The biochemical profile: LDC (+), CIT (+), URE (+), IND (-), VP (+), GLU (+), MAN (+), INO (+), SOR (+), RHA (+), SAC (+), MEL (+), ARA (+) was found in all services. There is a high frequency of Klebsiella pneumoniae pneumonia with several different digital profiles, so it is still essential to make a good identification to facilitate the epidemiological data.

Published in	European Journal of Clinical and Biomedical Sciences (Volume 5, Issue 3)
DOI	10.11648/j.ejcbs.20190503.12
Page(s)	46-50
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Gallery, Identification, Klebsiella Pneumoniae, Digital Profile

References

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[3]	Guessennd N, Kacou-N’doubaA, GbononV, YapiD, EkazaE, Dosso M et al. Prévalence et profil de résistance des entérobactéries productrices de beta-lactamasesa spectre élargi (BLSE) à Abidjan Côte d’ivoire de 2005 à 2006. J. sci. pharm. biol. 2008; 9 (1): 63-70.
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[5]	Freney J, Leclerq R, Riegel P. Précis de Bactériologie clinique. 2è édition. Paris: Editions ESKA; 2007. p. 1012.
[6]	Chafa-BetbeuiA, Gonsu-Kamga H, Toukam M, Mbakop CD, Enjema-Lyonga E, Bilong S et al. Phenotypic Detection of Extended Spectrum Beta-Lactamase and Carbapenemases Produced by Klebsiellaspp Isolated from Three Referrals Hospitals in Yaounde, Cameroon. BrMicrobiologyRes J. 2015; 9 (1): 1-9.
[7]	Zineb-Belbel. Evaluation de la résistance aux antibiotiques des souches deKlebsiellapneumoniaeisolées dans les hôpitaux de la ville d’Annaba. Thèse de Doctorat en microbiologie. Université Badji Mokhtar Annaba; 2014, 66-67.
[8]	Sekhri-Arafa. Fréquence et marqueurs épidémiologiques de Klebsiellapneumoniaedans les services à haut risque infectieux au niveau du CHU Benbadis de Constantine. Thèse de Doctorat en Biochimie et Microbiologie. Université Mentouri de Constantine; 2011, 92-96.
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[11]	Feizabadi MM, Mahamadi-Yeganeh S, Mirsalehian A, Mirafshar SM, Mahboobi M, Nili F, et al. Genetic characterization of ESBL producing strains of Klebsiella pneumoniae fromTehran hospitals. J Infect Dev Ctries. 2010; 4 (10): 609-615.
[12]	Dedeic-Ljubovic A, Hukic M, Pfeifer Y, Witte W, Padilla E, López-Ramis I, Albertí S. Emergence of CTX-M-15 extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolates in Bosnia and Herzegovina. Clin Microbiol Infect. 2010; 16 (2): 152-6.
[13]	Elhani D, Bakir L, Mahjoub A. Changement de l’épidémiologie de Klebsiella pneumoniae productrice de β-lactamases à spectre élargi. J Pat Bio. 2011; 69 (5): 523-9.
[14]	Djuikoue IC, Woerther PL, Toukam M, Burdet C, Ruppé E, Gonsu KH et al. Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon. J Infect Dev Ctries. 2016 Oct 31; 10 (10): 1135-1139.
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APA Style

Jean Teghonong, Cecile Okalla Ebongue, Callixte Yadufashije, Dieudonne Adiogo. (2019). Digital Profile of Klebsiella Pneumoniae Strains Producing Extended Spectrum β-Lactamase Isolated in Hospitals in the Littoral Region of Cameroon. European Journal of Clinical and Biomedical Sciences, 5(3), 46-50. https://doi.org/10.11648/j.ejcbs.20190503.12

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Jean Teghonong; Cecile Okalla Ebongue; Callixte Yadufashije; Dieudonne Adiogo. Digital Profile of Klebsiella Pneumoniae Strains Producing Extended Spectrum β-Lactamase Isolated in Hospitals in the Littoral Region of Cameroon. Eur. J. Clin. Biomed. Sci. 2019, 5(3), 46-50. doi: 10.11648/j.ejcbs.20190503.12

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AMA Style

Jean Teghonong, Cecile Okalla Ebongue, Callixte Yadufashije, Dieudonne Adiogo. Digital Profile of Klebsiella Pneumoniae Strains Producing Extended Spectrum β-Lactamase Isolated in Hospitals in the Littoral Region of Cameroon. Eur J Clin Biomed Sci. 2019;5(3):46-50. doi: 10.11648/j.ejcbs.20190503.12

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@article{10.11648/j.ejcbs.20190503.12,
  author = {Jean Teghonong and Cecile Okalla Ebongue and Callixte Yadufashije and Dieudonne Adiogo},
  title = {Digital Profile of Klebsiella Pneumoniae Strains Producing Extended Spectrum β-Lactamase Isolated in Hospitals in the Littoral Region of Cameroon},
  journal = {European Journal of Clinical and Biomedical Sciences},
  volume = {5},
  number = {3},
  pages = {46-50},
  doi = {10.11648/j.ejcbs.20190503.12},
  url = {https://doi.org/10.11648/j.ejcbs.20190503.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20190503.12},
  abstract = {Miniaturized galleries are essential for a good identification of bacteria. The aim of this study was to identify multi-resistant Klebsiellap neumoniae strains in the referral health structures of Cameroon littoral region and establish a digital profile. A cross-sectional and descriptivestudy was conducted during 2016 and early 2017, intermediate susceptibility isolates or resistant to cephalosporins third generation (C3G) were collected in the laboratories of referral hospitals of the Littoral (General Hospital, Laquintinie, Military, Gyneco-obstetrics and pediatrics of Douala and Saint John of Malta of Njombé) and in Diagmed laboratory. The confirmation of the strains was made by the gallery API 20E™ (BioMérieux). In this study, atotal of 412 strains of Klebsiellasp (species) were isolated. We obtained 125 strains of Klebsiellasp intermediate or resistant to (C3G) and 122 were confirmed to be Klebsiella pneumoniae. The sex ratio (F / M) was 1.26. Klebsiella pneumoniae pneumonia was the species mostly identified at 82.4% and the two digital profiles mainly found were 5215773 and 1215773 with respective frequencies of 52.8% and 18.4%. The biochemical profile: LDC (+), CIT (+), URE (+), IND (-), VP (+), GLU (+), MAN (+), INO (+), SOR (+), RHA (+), SAC (+), MEL (+), ARA (+) was found in all services. There is a high frequency of Klebsiella pneumoniae pneumonia with several different digital profiles, so it is still essential to make a good identification to facilitate the epidemiological data.},
 year = {2019}
}

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TY  - JOUR
T1  - Digital Profile of Klebsiella Pneumoniae Strains Producing Extended Spectrum β-Lactamase Isolated in Hospitals in the Littoral Region of Cameroon
AU  - Jean Teghonong
AU  - Cecile Okalla Ebongue
AU  - Callixte Yadufashije
AU  - Dieudonne Adiogo
Y1  - 2019/07/24
PY  - 2019
N1  - https://doi.org/10.11648/j.ejcbs.20190503.12
DO  - 10.11648/j.ejcbs.20190503.12
T2  - European Journal of Clinical and Biomedical Sciences
JF  - European Journal of Clinical and Biomedical Sciences
JO  - European Journal of Clinical and Biomedical Sciences
SP  - 46
EP  - 50
PB  - Science Publishing Group
SN  - 2575-5005
UR  - https://doi.org/10.11648/j.ejcbs.20190503.12
AB  - Miniaturized galleries are essential for a good identification of bacteria. The aim of this study was to identify multi-resistant Klebsiellap neumoniae strains in the referral health structures of Cameroon littoral region and establish a digital profile. A cross-sectional and descriptivestudy was conducted during 2016 and early 2017, intermediate susceptibility isolates or resistant to cephalosporins third generation (C3G) were collected in the laboratories of referral hospitals of the Littoral (General Hospital, Laquintinie, Military, Gyneco-obstetrics and pediatrics of Douala and Saint John of Malta of Njombé) and in Diagmed laboratory. The confirmation of the strains was made by the gallery API 20E™ (BioMérieux). In this study, atotal of 412 strains of Klebsiellasp (species) were isolated. We obtained 125 strains of Klebsiellasp intermediate or resistant to (C3G) and 122 were confirmed to be Klebsiella pneumoniae. The sex ratio (F / M) was 1.26. Klebsiella pneumoniae pneumonia was the species mostly identified at 82.4% and the two digital profiles mainly found were 5215773 and 1215773 with respective frequencies of 52.8% and 18.4%. The biochemical profile: LDC (+), CIT (+), URE (+), IND (-), VP (+), GLU (+), MAN (+), INO (+), SOR (+), RHA (+), SAC (+), MEL (+), ARA (+) was found in all services. There is a high frequency of Klebsiella pneumoniae pneumonia with several different digital profiles, so it is still essential to make a good identification to facilitate the epidemiological data.
VL  - 5
IS  - 3
ER  -

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Author Information

Jean Teghonong

Department of Biological Sciences, Distant Production House University, Uvira, Democratic Republic of Congo; Diagmed Laboratory, Douala, Cameroon
Cecile Okalla Ebongue

Department of Biological Sciences, Douala University, Douala, Cameroon
Callixte Yadufashije

INES-Ruhengeri - Institute of Applied Sciences, Musanze, Rwanda
Dieudonne Adiogo

Department of Biological Sciences, Douala University, Douala, Cameroon

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Plain Text BibTeX RIS

APA Style

Jean Teghonong, Cecile Okalla Ebongue, Callixte Yadufashije, Dieudonne Adiogo. (2019). Digital Profile of Klebsiella Pneumoniae Strains Producing Extended Spectrum β-Lactamase Isolated in Hospitals in the Littoral Region of Cameroon. European Journal of Clinical and Biomedical Sciences, 5(3), 46-50. https://doi.org/10.11648/j.ejcbs.20190503.12

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ACS Style

Jean Teghonong; Cecile Okalla Ebongue; Callixte Yadufashije; Dieudonne Adiogo. Digital Profile of Klebsiella Pneumoniae Strains Producing Extended Spectrum β-Lactamase Isolated in Hospitals in the Littoral Region of Cameroon. Eur. J. Clin. Biomed. Sci. 2019, 5(3), 46-50. doi: 10.11648/j.ejcbs.20190503.12

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AMA Style

Jean Teghonong, Cecile Okalla Ebongue, Callixte Yadufashije, Dieudonne Adiogo. Digital Profile of Klebsiella Pneumoniae Strains Producing Extended Spectrum β-Lactamase Isolated in Hospitals in the Littoral Region of Cameroon. Eur J Clin Biomed Sci. 2019;5(3):46-50. doi: 10.11648/j.ejcbs.20190503.12

Copy | Download

@article{10.11648/j.ejcbs.20190503.12,
  author = {Jean Teghonong and Cecile Okalla Ebongue and Callixte Yadufashije and Dieudonne Adiogo},
  title = {Digital Profile of Klebsiella Pneumoniae Strains Producing Extended Spectrum β-Lactamase Isolated in Hospitals in the Littoral Region of Cameroon},
  journal = {European Journal of Clinical and Biomedical Sciences},
  volume = {5},
  number = {3},
  pages = {46-50},
  doi = {10.11648/j.ejcbs.20190503.12},
  url = {https://doi.org/10.11648/j.ejcbs.20190503.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20190503.12},
  abstract = {Miniaturized galleries are essential for a good identification of bacteria. The aim of this study was to identify multi-resistant Klebsiellap neumoniae strains in the referral health structures of Cameroon littoral region and establish a digital profile. A cross-sectional and descriptivestudy was conducted during 2016 and early 2017, intermediate susceptibility isolates or resistant to cephalosporins third generation (C3G) were collected in the laboratories of referral hospitals of the Littoral (General Hospital, Laquintinie, Military, Gyneco-obstetrics and pediatrics of Douala and Saint John of Malta of Njombé) and in Diagmed laboratory. The confirmation of the strains was made by the gallery API 20E™ (BioMérieux). In this study, atotal of 412 strains of Klebsiellasp (species) were isolated. We obtained 125 strains of Klebsiellasp intermediate or resistant to (C3G) and 122 were confirmed to be Klebsiella pneumoniae. The sex ratio (F / M) was 1.26. Klebsiella pneumoniae pneumonia was the species mostly identified at 82.4% and the two digital profiles mainly found were 5215773 and 1215773 with respective frequencies of 52.8% and 18.4%. The biochemical profile: LDC (+), CIT (+), URE (+), IND (-), VP (+), GLU (+), MAN (+), INO (+), SOR (+), RHA (+), SAC (+), MEL (+), ARA (+) was found in all services. There is a high frequency of Klebsiella pneumoniae pneumonia with several different digital profiles, so it is still essential to make a good identification to facilitate the epidemiological data.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Digital Profile of Klebsiella Pneumoniae Strains Producing Extended Spectrum β-Lactamase Isolated in Hospitals in the Littoral Region of Cameroon
AU  - Jean Teghonong
AU  - Cecile Okalla Ebongue
AU  - Callixte Yadufashije
AU  - Dieudonne Adiogo
Y1  - 2019/07/24
PY  - 2019
N1  - https://doi.org/10.11648/j.ejcbs.20190503.12
DO  - 10.11648/j.ejcbs.20190503.12
T2  - European Journal of Clinical and Biomedical Sciences
JF  - European Journal of Clinical and Biomedical Sciences
JO  - European Journal of Clinical and Biomedical Sciences
SP  - 46
EP  - 50
PB  - Science Publishing Group
SN  - 2575-5005
UR  - https://doi.org/10.11648/j.ejcbs.20190503.12
AB  - Miniaturized galleries are essential for a good identification of bacteria. The aim of this study was to identify multi-resistant Klebsiellap neumoniae strains in the referral health structures of Cameroon littoral region and establish a digital profile. A cross-sectional and descriptivestudy was conducted during 2016 and early 2017, intermediate susceptibility isolates or resistant to cephalosporins third generation (C3G) were collected in the laboratories of referral hospitals of the Littoral (General Hospital, Laquintinie, Military, Gyneco-obstetrics and pediatrics of Douala and Saint John of Malta of Njombé) and in Diagmed laboratory. The confirmation of the strains was made by the gallery API 20E™ (BioMérieux). In this study, atotal of 412 strains of Klebsiellasp (species) were isolated. We obtained 125 strains of Klebsiellasp intermediate or resistant to (C3G) and 122 were confirmed to be Klebsiella pneumoniae. The sex ratio (F / M) was 1.26. Klebsiella pneumoniae pneumonia was the species mostly identified at 82.4% and the two digital profiles mainly found were 5215773 and 1215773 with respective frequencies of 52.8% and 18.4%. The biochemical profile: LDC (+), CIT (+), URE (+), IND (-), VP (+), GLU (+), MAN (+), INO (+), SOR (+), RHA (+), SAC (+), MEL (+), ARA (+) was found in all services. There is a high frequency of Klebsiella pneumoniae pneumonia with several different digital profiles, so it is still essential to make a good identification to facilitate the epidemiological data.
VL  - 5
IS  - 3
ER  -

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