International Journal of Biomedical Engineering and Clinical Science

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Phytochemical Analysis and Assessment of Antibacterial and Antioxidant Activities of Phytolacca dodecandra L. Herit Leaf Extracts (Phytolaccaceae)

Received: 23 August 2019    Accepted: 26 September 2019    Published: 11 October 2019
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Abstract

From times immemorial, medicinal plants have been used to relieve and cure human diseases. Currently, the control of bacterial infections is becoming complex due to the concern of antibiotic resistance, which has been a significant global health problem. The aim of this work was to determine the phytochemical composition as well as to assess the bioactivities of Phytolacca dodecandra leaf extracts. The leaves of P. dodecandra were collected in January 2017 at Yakoma city, Nord-Ubangi, DRC. Three bacterial strains namely Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 8739 and Pseudomonas aeroginosa ATCC 9027 were used for the assessment of the antibacterial activity. The qualitative and quantitative phytochemical screening were used for compound identification. The antioxidant activity was assessed using ABTS and DPPH scavenging tests while the antibacterial activity was performed using the diffusion method. The findings show that leaves of this plant are rich in flavonoids, anthocyanins, terpenoids and irrioids. The inhibitory concentration 50 (IC50) values obtained in the ATBS test are lower than those of the DPPH test. The antibacterial activity of this plant was low against the three strains used. These in vitro findings show that medicinal plants have very important biological properties that have many applications in various fields such as medicine, pharmacy and agriculture.

DOI 10.11648/j.ijbecs.20190503.11
Published in International Journal of Biomedical Engineering and Clinical Science (Volume 5, Issue 3, September 2019)
Page(s) 31-39
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Phytolacca dodecandra, Antibiotic Resistance, Bioactivity, Yakoma, DRC

References
[1] Mourad B. (2011). Etude de l’activité antioxydante et antimicrobienne d’extraits d’Artemisia campestris L. Mémoire pour l’obtention du diplôme de Magister, Université Ferhat Abbes-Setif.
[2] WHO (World Health Organization) (2002). Rapport sur la médecine traditionnelle: besoins et potentiels, N°4, 6pp.
[3] Biyiti L. F., Meko'o DJ. L, Tamzc v., Amvam Zollo P. H. (2004). Recherche de l'Activité Antibactérienne de Quatre Plantes Médicinales Camerounaises. Mémoire de licence Biochimie. Université de Yaoundé 1.
[4] Karou D., Mamoudou H. Dicko, Jacques Simporé, Yameogo S., Souleymane Sanon, Alfred S. Traoré. (2005). Activités antioxydantes et antibactériennes des polyphénols extraits de plantes médicinales de la pharmacopée traditionnelle du Burkina Faso. 4 p.
[5] Benbrinis S. (2012). Evaluation des activités antioxydante et antibactérienne des extraits de Santolina chamaecyparissus. Mémoire pour l’obtention du Diplôme de Magister en biochimie. Universite Ferhat Abbas-Setif/Algerie.
[6] Boutlelis DA (2014). Etude phytochimique et activité antimicrobienne, antioxydante, antihépatotoxique du Marrube blanc ou Marrubium vulgare L. Thèse en vue de l’obtention du diplôme de doctorat en science. Universite Badji Mokhtar – Annaba/Algerie.
[7] Dupuis B (2010). Plantes utiles en Afrique. Mémoire de licence Médecin Kjell B.
[8] Esser KB, Semagn K and Wolde-Yohannes L. (2003). Medicinal use and social status of the soap berry endod (Phytolacca dodecandra) in Ethiopia. Journal of Ethnopharmacology, 85 (2-3): 269–277.
[9] Inkoto CL, Bongo GN, Mutwale PK, Masengo CA, Gbolo B Z, Tshiama C, Ngombe NK, Iteku JB, Mbemba TF, Mpiana PT and Ngbolua KN (2018). Microscopic features and chromatographic fingerprints of selected Congolese medicinal plants: Aframomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels. Emergent Life Sciences Research, 4 (1): 1-10.
[10] Sofowora A (1993), Medicinal plant and traditional medicine in Africa, Spectrum Books Ltd, Ibadan, Nigeria, p. 270-289.
[11] Harbone JB. Methods of extraction and isolation ‘Phytochemical Methods. 3rd Edition Chapman and Hall, London. 1998; 60-66.
[12] Mbadiko CM, Bongo GN, Mindele LU, Ngbolua KN, Mpiana PT, Ngombe NK, Mutwale PK and Mbemba TF (2019). Effect of drying on the composition of secondary metabolites in extracts from floral parts of Curcuma longa L. Asian Journal of Research in Botany, 2 (2): 1-6.
[13] Tiwari P, Kumar B, Kaur M, Kaur G and Kaur H (2011). Phytochemical screening and extraction: A review. International Journal of Pharmaceutical Sciences, 1: 98-106.
[14] Bahati LM, Mutwale PM, Ngombe NK, Moni B, Makengo GK, Mungitshi PM, Pambu A, Bongo G, Tujibikila AM, Kabeya JK, Mbadiko CM, Frederich M. and Mbemba FT (2017). Microscopic Features, Chromatographic Fingerprints and Antioxidant Property of Some Unconventional Green Leafy Vegetables Consumed in Bandundu, DR Congo. Pharmacognosy Communication, 7 (4): 158-163.
[15] Kapepula PM, Mungitshi PM, Franck T, Ngoyi DM, Kalenda PDT, Ngombe, NK Ngombe, Serteyn D, Tits M, Frédérich M, Tamfum JJ. (2017). Antioxidant potentiality of three herbal teas consumed in Bandundu rural areas of Congo Antioxidant potentiality of three herbal teas consumed in. Natural Product Research. 31 (16): 1940–1943.
[16] Adedapo AA, Jimoh FO, Koduru S, Afolayan AJ, Masika PJ. (2008). Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of Calpurnia aurea. BMC Complementary and Alternative Medicine. 8: 1–8.
[17] Bongo G, Inkoto C, Masengo C, Tshiama C, Lengbiye E, Djolu R, Kapepula M, Ngombe K, Mbemba T, Tshilanda D, Mpiana P, Ngbolua KN (2017). Antisickling, Antioxidant and Antibacterial Activities of Afromomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels. American Journal of Laboratory Medicine, 2 (4): 52-59.
[18] Sochor J, Ryvolova M, Krystofova O, Salas P, Hubalek J, Adam V, Trnkova L, Havel L, Beklova M, Zehnalek J, Provaznik I and Kizek R (2010). Automated Spectrometric Protocols for Determination of Antioxidant Activity: Advantages and Disadvantages. Journal of Molecules, (15): 8618-8640.
[19] Ananil K., Hudson J.B. de Souzal C., Akpaganal K., Towe G. H. N., AmasonJ. T. and Gbeassor. (2000) Investigation of medicinal plants of Togo for antiviral and antimicrobial activities. Phannaceutical Biology, 38 (1): 40-45.
[20] Ngbolua, K, Mubindukila, REN., Mpiana, PT, Tshibangu, DST, & Ashande, M. C. (2014). Antibacterial and Antioxidant Activities of Anthocleista liebrechtsiana Wild & T. Durand (Gentianaceae) Originated from Democratic Republic of the Congo. Journal of advancement in medical and life sciences, 2014c, 1 (1): 1–6.
[21] Mekonnen N., Makonnen E., Aklilu N., Amen G. (2012). Evaluation of berries of Phytolacca dodecandra for growth inhibition of Histoplasma capsulatum var. farciminosum and treatment of cases of epizootic lymphangitis in Ethiopia. Asian Pacific Journal of Tropical Biomedicine, 2 (7): 505-510.
[22] Kuete V, Ngameni B, Simo CCF, Tankeu RK, Ngadjui BT, Meyer JJM, Lall N, Kuiate JR. (2008). Antimicrobial activity of the crude extracts and compounds from Ficus chlamydocarpa and Ficuscordata (Moraceae). Journal of Ethnopharmacology, 120: 17–24.
[23] Kuete V. (2010) Potential of Cameroonian plants and derived products against microbial infections: a review. Planta Med, 76 (14): 1479–1491.
[24] Grassmann J (2005). Terpenoids as Plant Antioxidants. Vitamins and Hormones, 72 (5), 505–535.
[25] Matebie WA, Zhang W and Xie G (2019). Chemical composition and antimicrobial activity of essential oil from Phytolacca dodecandra collected in Ethiopia. Molecules, 24 (342): 1-8.
[26] Tura GT, Eshete WB and Tuchi GT (2017). Antibacterial efficacy of local plants and their contribution to public health in rural Ethiopia. Antimicrobial Resistance and Infection Control, 6: 76 doi: 10.1186/s13756-017-0236-6.
[27] Vithya ED, Maleeka BSF and Ravikumar K (2018). Evaluation of phytochemical and antimicrobial property of Phytolacca octandra. International Journal of Research in Ayurveda Pharmacy, 9 (6): 111-115.
[28] Bukatuka, F, Ngombe, K, Mutwale, K, Moni B, Makengo K, Pambu L, Bongo NG, Mbombo MP, Musuyu MD, Maloueki U, Koto-te-Nyiwa and Mbemba, F (2016). Bioactivity and Nutritional Values of Some Dioscorea Species Traditionally Used as Medicinal Foods in Bandundu, DR Congo. European Journal of Medicinal Plants, 14 (1), 1–11.
[29] Inkoto A. (2017). Etudes microscopique, photochimique et bio-activités de Aframomum aloboviolaceum, Annona senegalensis et Mondia whitei de la pharmacopée Congolaise. Mémoire de licence biologie. UNIKIN/RDC.
[30] Imene R. (2013). Etude in vitro de l’activité anti Leishmanienne de certaines plantes médicinales locales: cas de la famille deslamiacées. Thèse en vue d’obtention du diplôme : Magister en Biologie Appliquée. Université Constantine 1’Algerie.
[31] El Gharras, H. (2009). Polyphenols: Food sources, properties and applications - A review. International Journal of Food Science and Technology, 44 (12): 2512–2518.
[32] Perron, N. R., & Brumaghim, J. L. (2009). A review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell Biochemistry and Biophysics, 53 (2), 75–100.
[33] Vauzour, D., Vafeiadou, K., Rodriguez-Mateos, A., Rendeiro, C., & Spencer, J. P. E. (2008). The neuroprotective potential of flavonoids: A multiplicity of effects. Genes and Nutrition, 3 (3-4), 115–126.
[34] Taye B, Giday M, Animut A and Seid J. (2011). Antibacterial activities of selected medicinal plants in traditional treatment of human wounds in Ethiopia. Asian Pacific Journal of Tropical Biomedicine, 1 (5): 370-375.
[35] Tadeg H., Mohammed E., Asres K., Gebre-Mariam T. (2005). Antimicrobial activities of some selected traditional Ethiopian medicinal plants used in the treatment of skin disorders. Journal of Ethnopharmacology, 100 (1-2): 168–175.
Author Information
  • Higher Pedagogical Institute of Yakoma, Nord-Ubangi City, Democratic Republic of the Congo; Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo

  • Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo

  • Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo

  • Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo

  • Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo

  • Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo

  • Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo

  • Higher Pedagogical Institute of Yakoma, Nord-Ubangi City, Democratic Republic of the Congo

  • Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo; Department of Environmental Sciences, University of Gbadolite, Nord-Ubangi City, Democratic Republic of the Congo

Cite This Article
  • APA Style

    Jeff Bekomo Iteku, Ornella Mbayi, Gedeon Ngiala Bongo, Paulin Kapepula Mutwale, Jose Mulwahali Wambale, et al. (2019). Phytochemical Analysis and Assessment of Antibacterial and Antioxidant Activities of Phytolacca dodecandra L. Herit Leaf Extracts (Phytolaccaceae). International Journal of Biomedical Engineering and Clinical Science, 5(3), 31-39. https://doi.org/10.11648/j.ijbecs.20190503.11

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    ACS Style

    Jeff Bekomo Iteku; Ornella Mbayi; Gedeon Ngiala Bongo; Paulin Kapepula Mutwale; Jose Mulwahali Wambale, et al. Phytochemical Analysis and Assessment of Antibacterial and Antioxidant Activities of Phytolacca dodecandra L. Herit Leaf Extracts (Phytolaccaceae). Int. J. Biomed. Eng. Clin. Sci. 2019, 5(3), 31-39. doi: 10.11648/j.ijbecs.20190503.11

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    AMA Style

    Jeff Bekomo Iteku, Ornella Mbayi, Gedeon Ngiala Bongo, Paulin Kapepula Mutwale, Jose Mulwahali Wambale, et al. Phytochemical Analysis and Assessment of Antibacterial and Antioxidant Activities of Phytolacca dodecandra L. Herit Leaf Extracts (Phytolaccaceae). Int J Biomed Eng Clin Sci. 2019;5(3):31-39. doi: 10.11648/j.ijbecs.20190503.11

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  • @article{10.11648/j.ijbecs.20190503.11,
      author = {Jeff Bekomo Iteku and Ornella Mbayi and Gedeon Ngiala Bongo and Paulin Kapepula Mutwale and Jose Mulwahali Wambale and Emmanuel Lengbiye and Clement Liyongo Inkoto and Samy Ngunde Ngunde and Koto-te-Nyiwa Ngbolua},
      title = {Phytochemical Analysis and Assessment of Antibacterial and Antioxidant Activities of Phytolacca dodecandra L. Herit Leaf Extracts (Phytolaccaceae)},
      journal = {International Journal of Biomedical Engineering and Clinical Science},
      volume = {5},
      number = {3},
      pages = {31-39},
      doi = {10.11648/j.ijbecs.20190503.11},
      url = {https://doi.org/10.11648/j.ijbecs.20190503.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijbecs.20190503.11},
      abstract = {From times immemorial, medicinal plants have been used to relieve and cure human diseases. Currently, the control of bacterial infections is becoming complex due to the concern of antibiotic resistance, which has been a significant global health problem. The aim of this work was to determine the phytochemical composition as well as to assess the bioactivities of Phytolacca dodecandra leaf extracts. The leaves of P. dodecandra were collected in January 2017 at Yakoma city, Nord-Ubangi, DRC. Three bacterial strains namely Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 8739 and Pseudomonas aeroginosa ATCC 9027 were used for the assessment of the antibacterial activity. The qualitative and quantitative phytochemical screening were used for compound identification. The antioxidant activity was assessed using ABTS and DPPH scavenging tests while the antibacterial activity was performed using the diffusion method. The findings show that leaves of this plant are rich in flavonoids, anthocyanins, terpenoids and irrioids. The inhibitory concentration 50 (IC50) values obtained in the ATBS test are lower than those of the DPPH test. The antibacterial activity of this plant was low against the three strains used. These in vitro findings show that medicinal plants have very important biological properties that have many applications in various fields such as medicine, pharmacy and agriculture.},
     year = {2019}
    }
    

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    T1  - Phytochemical Analysis and Assessment of Antibacterial and Antioxidant Activities of Phytolacca dodecandra L. Herit Leaf Extracts (Phytolaccaceae)
    AU  - Jeff Bekomo Iteku
    AU  - Ornella Mbayi
    AU  - Gedeon Ngiala Bongo
    AU  - Paulin Kapepula Mutwale
    AU  - Jose Mulwahali Wambale
    AU  - Emmanuel Lengbiye
    AU  - Clement Liyongo Inkoto
    AU  - Samy Ngunde Ngunde
    AU  - Koto-te-Nyiwa Ngbolua
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    DO  - 10.11648/j.ijbecs.20190503.11
    T2  - International Journal of Biomedical Engineering and Clinical Science
    JF  - International Journal of Biomedical Engineering and Clinical Science
    JO  - International Journal of Biomedical Engineering and Clinical Science
    SP  - 31
    EP  - 39
    PB  - Science Publishing Group
    SN  - 2472-1301
    UR  - https://doi.org/10.11648/j.ijbecs.20190503.11
    AB  - From times immemorial, medicinal plants have been used to relieve and cure human diseases. Currently, the control of bacterial infections is becoming complex due to the concern of antibiotic resistance, which has been a significant global health problem. The aim of this work was to determine the phytochemical composition as well as to assess the bioactivities of Phytolacca dodecandra leaf extracts. The leaves of P. dodecandra were collected in January 2017 at Yakoma city, Nord-Ubangi, DRC. Three bacterial strains namely Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 8739 and Pseudomonas aeroginosa ATCC 9027 were used for the assessment of the antibacterial activity. The qualitative and quantitative phytochemical screening were used for compound identification. The antioxidant activity was assessed using ABTS and DPPH scavenging tests while the antibacterial activity was performed using the diffusion method. The findings show that leaves of this plant are rich in flavonoids, anthocyanins, terpenoids and irrioids. The inhibitory concentration 50 (IC50) values obtained in the ATBS test are lower than those of the DPPH test. The antibacterial activity of this plant was low against the three strains used. These in vitro findings show that medicinal plants have very important biological properties that have many applications in various fields such as medicine, pharmacy and agriculture.
    VL  - 5
    IS  - 3
    ER  - 

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