Growth and Accumulation of Five Main Bioactive Components in the Roots of <i>Salvia miltiorrhiza</i> at Different Growth Stages and Using Different Culture Systems

Lilan Lu; Shilong Fu; Jian Feng; Jianhe Wei

doi:doi:10.11648/j.jdmp.20180403.11

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Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems

Lilan Lu, Shilong Fu, Jian Feng, Jianhe Wei

Received: 23 April 2018 Accepted: 21 May 2018 Published: 8 June 2018

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Abstract

To Growth and accumulation of five main bioactive components in the roots of Salvia miltiorrhiza at different growth stages and using different culture systems. We analyzed growth parameters and the accumulation of selected bioactive components in Salvia miltiorrhiza that was grown in quartz sand-pot (hydroponic culture), soil-pot, and field culture systems at 3 growth stages (flower, root swelling, and mature). The highest bioactive compound concentrations (danshensu (DSS), 0.618 mg·g^-1; salvianolic acid B (SAB), 52.5 mg·g^-1; cryptotanshinone (CTS), 0.617 mg·g^-1; tanshinoneⅡA (TSⅡA), 1.11 mg·g^-1; and total tanshinone (TTS), 2.5 mg·g^-1, at the mature stage) were present in the roots of plants grown in the hydroponic culture system. These concentrations were significantly higher than those of plants grown in the field system. The highest values for root parameters ( longest root length (LRL), 46.72 cm; largest root diameter (LRD), 14.68 mm; and the number of roots per plant (RN), 9.56), plant biomass (shoot dry weight (SDW), 18.9 g·plant^-1; root dry weight (RDW), 19.6 g·plant^-1, at the mature stage), and yield (DSS, 8.36 mg·plant^-1; SAB, 657 mg·plant^-1; CTS, 7.95 mg·plant^-1; TSⅡA, 15.2 mg·plant^-1; and TTS, 30.7 mg·plant^-1, at the mature stage) were obtained from plants grown in the field system. Plants grown in the field culture system had significantly greater plant biomass and higher yields of bioactive compounds than plants grown in the quartz sand-pot (hydroponic culture) and soil-pot systems. Greenhouse hydroponic culture provides sufficient bioactive compound accumulation in the roots, but does not stimulate plant growth and root production. Therefore, the field system could greatly improve plant growth and root production in S. miltiorrhiza.

DOI	10.11648/j.jdmp.20180403.11
Published in	Journal of Diseases and Medicinal Plants (Volume 4, Issue 3, June 2018)
Page(s)	59-68
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Salvia miltiorrhiza, Culture System, Growth Stages, Bioactive Components, Growth

References

[1]	Tan XH, Xue WJ, Ding XM, 2005. Application of Danshen injection on early stage of renal transplantation. Chinese Journal of Integrative Medicine, 25, 404-407.
[2]	Wu ZM, Wen T, Tan YF, LiuY, Ren F, Wu H, 2007. Effects of salvianolic acid A on oxidative stress and liver in jury induced by carbon tetrachloride in rats. Basic and Clinical Pharmacology Toxicology, 100, 115-120.
[3]	Lam FF, Yeung JH, Cheung JH, Or PM. 2006. Pharmacological evidence for calcium channel inhibition by Danshen (Salvia miltiorrhiza) on rat isolated femoral artery. Journal Cardiovascular Pharmacology, 47, 139-145.
[4]	Lin YL, WuC, Luo MH, Huang YJ, Wang CN, Shao MS, Huang YT, 2006. In Vitro protective effects of salvianolic acid B on primary hepatocytes and hepatic stellate cells. Journal of Ethnopharmacology, 105, 215-222.
[5]	Sze FK, Yeung FF, Wong E, Lau J, 2005. Does Danshen improve disability after ischaemic stroke? Acta Neurologica Scandinavica, 111, 118-125.
[6]	Zhang HS and Wang SQ, 2006. Salvianolic acid B from Salvia miltiorrhiza inhibits tumor necrosis factor-α (TNF-α)-induced MMP-2 up regulation in human aortic smooth muscle cells via suppression of NAD (P) H oxidase-derived reactive oxygen species. Journal of Molecular and Cellular Cardiology, 41, 138-148.
[7]	Heuberger HB, Bauer F, Friedl G, Heubl J, Hummelsberger R, Nögel R, Seidenberger P, Torres L, 2010. Cultivation and Breeding of Chinese Medicinal Plants in Germany. Planta Medica, 76, 1956-1962.
[8]	Xing ZJ and Xing Y, 2009. The analysis about the product and sale of Danshen. Available online: http:// www. bzzyc.cn/Article/Show.asp?ID=8163.
[9]	Dorais M, Papadopoulos AP, Luo X, Leonhart S, Gosselin A, Pedneault K, Angers P, Gaudreau L, 2001. Soilless greenhouse production of medicinal plants in North Eastern Canada. Acta Horticulturae: World Congress on Soilless Culture: Agriculture in the Coming Millennium, 554, 297-303.
[10]	Mairapetyan S and Manukyan A, 2002. Catmint culture. Practical Hydroponics and Greenhouses, 62, 63-65.
[11]	Manukyan A, 2001. The development of biotechnologies of celandine and catmint in outside hydroponics conditions, PhD diss. summary, 21. National Academy of Sciences of the Republic of Armenia (in Armenian).
[12]	Craker L, 2003. Production and demand: A view to the future of MAP, In: Proceedings of the International Conference on Medicinal and Aromatic Plants (Part II), Budapest Hungary 15-21.
[13]	Dufault RJ, Rushing J, Hassell T, Shepard BM, McCutcheon G, Ward B, 2003. Influence of fertilizer on growth and marker compound of field-grown Echinacea species and feverfew. Scientia Horticulturae, 98, 61-70.
[14]	Li MH, Wei JM, Peng Y, Wu QL, Xiao PG, 2008. Investigation of Danshen and related medicinal plants in China. Journal of Ethnopharmacology, 120, 419-426.
[15]	Hassanpouragham MB, Tabatabaie SJ, Nazemiyeh H, Vojodi L, Aazami MA, 2008. Essential oil composition of hydroponically grown Chrysanthemum balsamita L. Journal of Essential Oil-Bearing Plants, 11, 649-654.
[16]	Hassanpouragham MB, 2009. Flower heads volatile oil composition of soilless culture grown Chrysanthemum balsamita L. Natural Product Research, 23, 672-677.
[17]	Sellami, H. K., Napolitano, A., Masullo, M., Smiti, S., Piacente, S., & Pizza, C. 2013. Influence of growing conditions on metabolite profile of ammi visnaga, umbels with special reference to bioactive furanochromones and pyranocoumarins. Phytochemistry, 95(6), 197-206.
[18]	Treftz, C. 2015. Comparison of nutritional and sensory qualities between hydroponic and soil-grown strawberries and raspberries. Dissertations & Theses-Gradworks.
[19]	Treftz, C., & Omaye, S. T. 2015. Hydroponic raspberries: determining differences in yield, bioactive compounds, and taste preferences. Journal of the Academy of Nutrition & Dietetics, 115(9), A42-A42.
[20]	Maggini R., Tozzini L, Pacifici S, Raffaelli A, Pardossi A, 2012. Growth and accumulation of caffeic acid derivatives in Echinacea angustifolia DC. Var. angustifolia grown in hydroponics culture. Industrial Crops and Products, 35, 269-273.
[21]	Pardossi A, Malorgio F, Incrocci L, Tognoni F, 2005. Hydroponic technologies for greenhouse crops. In: Ramdane, D. (Ed.), Crops: Quality, Growth and Biotechnology. WFL Publisher, Helsinki, pp. 360-378.
[22]	Niu J. F, Peng YF, Li CJ, Zhang FS, 2010. Changes in root length at the reproductive stage of maize plants grown In the field and quartz sand. Journal Plant Nutrition Soil Science, 173, 306-314.
[23]	Pedneault K, Léonhart S, Gosselin A, Papadopoulos AP, Dorais M, Angers P, 2000. Variations in concentration of active compounds in four hydroponically- and field-grown medicinal plant species. In: Acta Horticulturae. 580: IV International ISHS Symposium on Artificial Lighting, Plenum Press, New York, pp. 97-175.
[24]	Matkowski A, Zielinska S, Oszmianski J, Lamer-Zarawska E, 2008. Antioxidant activity of extracts from leaves and roots of Salvia miltiorrhiza Bunge, S. przewalskii Maxim, and S. verticillata L. Bioresource Technology, 99, 7892-7896.
[25]	Dong JE, Liu YB, Liang ZS, Wang WL, 2010. Investigation on ultrasound-assisted extraction of salvianolic acid B from Salvia miltiorrhiza root. Ultrasonics Sonochemistry, 17, 61-65.
[26]	Lu YR and Foo LY, 2002. Polyphenolics of Salvia—a review. Phytochemistry, 59, 117-140.
[27]	Hoagland DR and Arnon D I, 1950. The water-culture method for growing plants without soil. 2nd edit. Circular. Agricultural Experiment Station, University of California, Berkeley. pp. 32.
[28]	Lu LL, Hou S, Zheng TT, Zhang, XL and Wei JH, 2015. Simultaneous determination of five hydrophilic and lipophilic components from roots of salvia miltiorrhiza by hplc. Chinese Herbal Medicines, 7(1), 75-79.
[29]	Kirakosyan A, Kaufman P, Warber S, Zick S, Aaronson K, Bolling S, Chang SC, 2004. Applied environmental stresses to enhance the levels of polyphenolics in leaves of hawthorn plants. Physiologia Plantarum, 121, 182-186.
[30]	Zhu ZZ, Liang ZS, Han RL, Wang X, 2009. Impact of fertilization on drought response in the medicinal herb Bupleurum chinense DC.: Growth and saikosaponin production. Industrial Crops and Products, 29, 629-633.
[31]	Marcus IM, Bolster CH, Cook KL, Opot SR, Walker SL, 2012. Impact of growth conditions on transport behavior of E. coli. Journal of Environmental Monitoring, 14, 984-991.
[32]	Chrishaki N and Horiguchi T, 1997. Responses of secondary metabolism in plants to nutrient deficiency. Soil Science Plant Nutrition, 43, 987-91.
[33]	Rosa TM, Julkunen TR, Lehto1T, Aphalol PJ, 2001. Secondary metabolites and nutrient concentrations in silver birch seedlings under five levels of daily UV-B exposure and two relative nutrient addition rates. New Phytologist, 150, 121-131.
[34]	Gleadow RM and Woodrow IE, 2002. Defense chemistry of cyanogenic Eucalyptus cladocalyx seedlings is affected by water supply. Tree Physiology, 22, 939-986.
[35]	Suh E, Park K, Park K, 1999. Effect of different concentrations of nutrient solutions on the growth, yield, and quality of basil. Acta Horticulturae, 502, 56-61.
[36]	Udagawa Y, 1995. Some responses of dill (Anethum graveolens) and thyme (Thymus vulgaris), grown in hydroponic, to the concentration of nutrient solution. Acta Horticulturae, 396, 203-210.
[37]	He CE, Wei JH, Jin Y, Chen SL, 2010. Bioactive component of the roots of Salvia miltiorrhizae: Changes related to harvest time and germplasms line. Industrial Crops Products, 32, 313-317.
[38]	Schlick G, 2000. Nutritional characteristics and biomasses production of Chenopodium quinoa grown in controlled environments. San Jose State University, Master's Theses. Paper, 2112, pp. 1-35.
[39]	Manukyan AE, Heuberger HT, Schnitzler WH, 2004. Yield and quality of some herbs of the Lamiaceae family under soilless greenhouse production. Journal of Applied Botany and Food Quality, 78, 193-199.
[40]	Mairapetyan SK, 1999. Aromatic plant culture in open-air hydroponics. Acta Horticulturae. II WOCMAP Congress Medicinal and Aromatic Plants, Part 3: Agricultural Production, Post Harvest Techniques, Biotechnology, 503, 33-42.
[41]	The Pharmacopoeia Commission of PRC, 2010. Pharmacopoeia of the People’s Republic of China Version 2010, vol. 1. Chinese medicine science technology Press, Beijing, pp. 72.

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Lilan Lu, Shilong Fu, Jian Feng, Jianhe Wei. (2018). Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems. Journal of Diseases and Medicinal Plants, 4(3), 59-68. https://doi.org/10.11648/j.jdmp.20180403.11

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ACS Style

Lilan Lu; Shilong Fu; Jian Feng; Jianhe Wei. Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems. J. Dis. Med. Plants 2018, 4(3), 59-68. doi: 10.11648/j.jdmp.20180403.11

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AMA Style

Lilan Lu, Shilong Fu, Jian Feng, Jianhe Wei. Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems. J Dis Med Plants. 2018;4(3):59-68. doi: 10.11648/j.jdmp.20180403.11

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@article{10.11648/j.jdmp.20180403.11,
  author = {Lilan Lu and Shilong Fu and Jian Feng and Jianhe Wei},
  title = {Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {4},
  number = {3},
  pages = {59-68},
  doi = {10.11648/j.jdmp.20180403.11},
  url = {https://doi.org/10.11648/j.jdmp.20180403.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20180403.11},
  abstract = {To Growth and accumulation of five main bioactive components in the roots of Salvia miltiorrhiza at different growth stages and using different culture systems. We analyzed growth parameters and the accumulation of selected bioactive components in Salvia miltiorrhiza that was grown in quartz sand-pot (hydroponic culture), soil-pot, and field culture systems at 3 growth stages (flower, root swelling, and mature). The highest bioactive compound concentrations (danshensu (DSS), 0.618 mg·g-1; salvianolic acid B (SAB), 52.5 mg·g-1; cryptotanshinone (CTS), 0.617 mg·g-1; tanshinoneⅡA (TSⅡA), 1.11 mg·g-1; and total tanshinone (TTS), 2.5 mg·g-1, at the mature stage) were present in the roots of plants grown in the hydroponic culture system. These concentrations were significantly higher than those of plants grown in the field system. The highest values for root parameters ( longest root length (LRL), 46.72 cm; largest root diameter (LRD), 14.68 mm; and the number of roots per plant (RN), 9.56), plant biomass (shoot dry weight (SDW), 18.9 g·plant-1; root dry weight (RDW), 19.6 g·plant-1, at the mature stage), and yield (DSS, 8.36 mg·plant-1; SAB, 657 mg·plant-1; CTS, 7.95 mg·plant-1; TSⅡA, 15.2 mg·plant-1; and TTS, 30.7 mg·plant-1, at the mature stage) were obtained from plants grown in the field system. Plants grown in the field culture system had significantly greater plant biomass and higher yields of bioactive compounds than plants grown in the quartz sand-pot (hydroponic culture) and soil-pot systems. Greenhouse hydroponic culture provides sufficient bioactive compound accumulation in the roots, but does not stimulate plant growth and root production. Therefore, the field system could greatly improve plant growth and root production in S. miltiorrhiza.},
 year = {2018}
}

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TY  - JOUR
T1  - Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems
AU  - Lilan Lu
AU  - Shilong Fu
AU  - Jian Feng
AU  - Jianhe Wei
Y1  - 2018/06/08
PY  - 2018
N1  - https://doi.org/10.11648/j.jdmp.20180403.11
DO  - 10.11648/j.jdmp.20180403.11
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 59
EP  - 68
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20180403.11
AB  - To Growth and accumulation of five main bioactive components in the roots of Salvia miltiorrhiza at different growth stages and using different culture systems. We analyzed growth parameters and the accumulation of selected bioactive components in Salvia miltiorrhiza that was grown in quartz sand-pot (hydroponic culture), soil-pot, and field culture systems at 3 growth stages (flower, root swelling, and mature). The highest bioactive compound concentrations (danshensu (DSS), 0.618 mg·g-1; salvianolic acid B (SAB), 52.5 mg·g-1; cryptotanshinone (CTS), 0.617 mg·g-1; tanshinoneⅡA (TSⅡA), 1.11 mg·g-1; and total tanshinone (TTS), 2.5 mg·g-1, at the mature stage) were present in the roots of plants grown in the hydroponic culture system. These concentrations were significantly higher than those of plants grown in the field system. The highest values for root parameters ( longest root length (LRL), 46.72 cm; largest root diameter (LRD), 14.68 mm; and the number of roots per plant (RN), 9.56), plant biomass (shoot dry weight (SDW), 18.9 g·plant-1; root dry weight (RDW), 19.6 g·plant-1, at the mature stage), and yield (DSS, 8.36 mg·plant-1; SAB, 657 mg·plant-1; CTS, 7.95 mg·plant-1; TSⅡA, 15.2 mg·plant-1; and TTS, 30.7 mg·plant-1, at the mature stage) were obtained from plants grown in the field system. Plants grown in the field culture system had significantly greater plant biomass and higher yields of bioactive compounds than plants grown in the quartz sand-pot (hydroponic culture) and soil-pot systems. Greenhouse hydroponic culture provides sufficient bioactive compound accumulation in the roots, but does not stimulate plant growth and root production. Therefore, the field system could greatly improve plant growth and root production in S. miltiorrhiza.
VL  - 4
IS  - 3
ER  -

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Author Information

Lilan Lu

Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, China
Shilong Fu

Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, China
Jian Feng

Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, China
Jianhe Wei

Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, China; Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education & National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy

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APA Style

Lilan Lu, Shilong Fu, Jian Feng, Jianhe Wei. (2018). Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems. Journal of Diseases and Medicinal Plants, 4(3), 59-68. https://doi.org/10.11648/j.jdmp.20180403.11

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ACS Style

Lilan Lu; Shilong Fu; Jian Feng; Jianhe Wei. Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems. J. Dis. Med. Plants 2018, 4(3), 59-68. doi: 10.11648/j.jdmp.20180403.11

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AMA Style

Lilan Lu, Shilong Fu, Jian Feng, Jianhe Wei. Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems. J Dis Med Plants. 2018;4(3):59-68. doi: 10.11648/j.jdmp.20180403.11

Copy | Download

@article{10.11648/j.jdmp.20180403.11,
  author = {Lilan Lu and Shilong Fu and Jian Feng and Jianhe Wei},
  title = {Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {4},
  number = {3},
  pages = {59-68},
  doi = {10.11648/j.jdmp.20180403.11},
  url = {https://doi.org/10.11648/j.jdmp.20180403.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20180403.11},
  abstract = {To Growth and accumulation of five main bioactive components in the roots of Salvia miltiorrhiza at different growth stages and using different culture systems. We analyzed growth parameters and the accumulation of selected bioactive components in Salvia miltiorrhiza that was grown in quartz sand-pot (hydroponic culture), soil-pot, and field culture systems at 3 growth stages (flower, root swelling, and mature). The highest bioactive compound concentrations (danshensu (DSS), 0.618 mg·g-1; salvianolic acid B (SAB), 52.5 mg·g-1; cryptotanshinone (CTS), 0.617 mg·g-1; tanshinoneⅡA (TSⅡA), 1.11 mg·g-1; and total tanshinone (TTS), 2.5 mg·g-1, at the mature stage) were present in the roots of plants grown in the hydroponic culture system. These concentrations were significantly higher than those of plants grown in the field system. The highest values for root parameters ( longest root length (LRL), 46.72 cm; largest root diameter (LRD), 14.68 mm; and the number of roots per plant (RN), 9.56), plant biomass (shoot dry weight (SDW), 18.9 g·plant-1; root dry weight (RDW), 19.6 g·plant-1, at the mature stage), and yield (DSS, 8.36 mg·plant-1; SAB, 657 mg·plant-1; CTS, 7.95 mg·plant-1; TSⅡA, 15.2 mg·plant-1; and TTS, 30.7 mg·plant-1, at the mature stage) were obtained from plants grown in the field system. Plants grown in the field culture system had significantly greater plant biomass and higher yields of bioactive compounds than plants grown in the quartz sand-pot (hydroponic culture) and soil-pot systems. Greenhouse hydroponic culture provides sufficient bioactive compound accumulation in the roots, but does not stimulate plant growth and root production. Therefore, the field system could greatly improve plant growth and root production in S. miltiorrhiza.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems
AU  - Lilan Lu
AU  - Shilong Fu
AU  - Jian Feng
AU  - Jianhe Wei
Y1  - 2018/06/08
PY  - 2018
N1  - https://doi.org/10.11648/j.jdmp.20180403.11
DO  - 10.11648/j.jdmp.20180403.11
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 59
EP  - 68
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20180403.11
AB  - To Growth and accumulation of five main bioactive components in the roots of Salvia miltiorrhiza at different growth stages and using different culture systems. We analyzed growth parameters and the accumulation of selected bioactive components in Salvia miltiorrhiza that was grown in quartz sand-pot (hydroponic culture), soil-pot, and field culture systems at 3 growth stages (flower, root swelling, and mature). The highest bioactive compound concentrations (danshensu (DSS), 0.618 mg·g-1; salvianolic acid B (SAB), 52.5 mg·g-1; cryptotanshinone (CTS), 0.617 mg·g-1; tanshinoneⅡA (TSⅡA), 1.11 mg·g-1; and total tanshinone (TTS), 2.5 mg·g-1, at the mature stage) were present in the roots of plants grown in the hydroponic culture system. These concentrations were significantly higher than those of plants grown in the field system. The highest values for root parameters ( longest root length (LRL), 46.72 cm; largest root diameter (LRD), 14.68 mm; and the number of roots per plant (RN), 9.56), plant biomass (shoot dry weight (SDW), 18.9 g·plant-1; root dry weight (RDW), 19.6 g·plant-1, at the mature stage), and yield (DSS, 8.36 mg·plant-1; SAB, 657 mg·plant-1; CTS, 7.95 mg·plant-1; TSⅡA, 15.2 mg·plant-1; and TTS, 30.7 mg·plant-1, at the mature stage) were obtained from plants grown in the field system. Plants grown in the field culture system had significantly greater plant biomass and higher yields of bioactive compounds than plants grown in the quartz sand-pot (hydroponic culture) and soil-pot systems. Greenhouse hydroponic culture provides sufficient bioactive compound accumulation in the roots, but does not stimulate plant growth and root production. Therefore, the field system could greatly improve plant growth and root production in S. miltiorrhiza.
VL  - 4
IS  - 3
ER  -

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